SRR3548496_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	632170
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	3263	0.5161586282170936	TruSeq Adapter, Index 21 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1098	0.1736874574877011	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	854	0.1350902447126564	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	845	0.13366657702833099	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	842	0.13319202113355585	No Hit
AATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	685	0.1083569293069902	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2107739E-7	45.000004	1
AACCGCG	35	1.2107739E-7	45.000004	1
CTACACG	35	1.2107739E-7	45.000004	1
ATTTACG	25	3.8888065E-5	45.0	1
CTTCGAA	20	7.030875E-4	45.0	33
AACCGTC	20	7.030875E-4	45.0	17
AACGTAG	25	3.8888065E-5	45.0	1
TAATACG	20	7.030875E-4	45.0	1
TCGTTGT	20	7.030875E-4	45.0	11
GGTCGAT	20	7.030875E-4	45.0	9
CGTGCAT	20	7.030875E-4	45.0	18
ATTAACG	20	7.030875E-4	45.0	1
CGTAGAT	20	7.030875E-4	45.0	44
AATTACG	40	6.8066583E-9	45.0	1
CGGTCTA	20	7.030875E-4	45.0	31
ACGTAGA	20	7.030875E-4	45.0	43
ACGTACG	20	7.030875E-4	45.0	1
CGCGTAG	20	7.030875E-4	45.0	1
ACGAACC	20	7.030875E-4	45.0	17
ACGTTCC	30	2.1639462E-6	44.999996	18