##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	632170
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.954205356154198	33.0	31.0	34.0	30.0	34.0
2	32.162049765094835	34.0	31.0	34.0	30.0	34.0
3	32.288585348877675	34.0	31.0	34.0	30.0	34.0
4	35.90266542227565	37.0	35.0	37.0	35.0	37.0
5	35.705123621810586	37.0	35.0	37.0	33.0	37.0
6	35.7039783602512	37.0	35.0	37.0	33.0	37.0
7	35.717568059224575	37.0	35.0	37.0	35.0	37.0
8	35.88282740402107	37.0	35.0	37.0	35.0	37.0
9	37.48301089896705	39.0	37.0	39.0	35.0	39.0
10	37.113760539095495	39.0	37.0	39.0	33.0	39.0
11	37.0916984355474	39.0	37.0	39.0	33.0	39.0
12	36.9828432225509	39.0	37.0	39.0	33.0	39.0
13	36.95493933593812	39.0	37.0	39.0	33.0	39.0
14	38.16128414825126	40.0	37.0	41.0	33.0	41.0
15	38.132372621288575	40.0	37.0	41.0	33.0	41.0
16	38.0662764762643	40.0	37.0	41.0	33.0	41.0
17	38.08962620814022	40.0	37.0	41.0	33.0	41.0
18	38.07186674470475	40.0	37.0	41.0	33.0	41.0
19	38.05638040400525	40.0	37.0	41.0	33.0	41.0
20	37.971238749070665	40.0	37.0	41.0	33.0	41.0
21	37.932274546403654	40.0	37.0	41.0	33.0	41.0
22	37.91338722179161	40.0	37.0	41.0	33.0	41.0
23	37.80879826628913	40.0	37.0	41.0	33.0	41.0
24	37.68916747077527	40.0	36.0	41.0	33.0	41.0
25	37.670580698229905	40.0	36.0	41.0	33.0	41.0
26	37.6053039530506	40.0	36.0	41.0	33.0	41.0
27	37.54291092585855	40.0	36.0	41.0	32.0	41.0
28	37.47181928911527	39.0	36.0	41.0	32.0	41.0
29	37.30278406124935	39.0	36.0	41.0	32.0	41.0
30	37.324827182561656	39.0	36.0	41.0	32.0	41.0
31	37.16246262872329	39.0	36.0	41.0	31.0	41.0
32	37.14818007814354	39.0	35.0	41.0	31.0	41.0
33	37.084662353480866	39.0	35.0	41.0	31.0	41.0
34	36.9454766913963	39.0	35.0	41.0	31.0	41.0
35	36.88267238242878	39.0	35.0	41.0	31.0	41.0
36	36.71956752139456	39.0	35.0	41.0	30.0	41.0
37	36.62991442175364	39.0	35.0	40.0	30.0	41.0
38	36.62035686603287	39.0	35.0	40.0	30.0	41.0
39	36.50852302387016	39.0	35.0	40.0	30.0	41.0
40	36.350190613284404	39.0	35.0	40.0	30.0	41.0
41	36.253604252020814	38.0	35.0	40.0	30.0	41.0
42	36.20155970704083	38.0	35.0	40.0	30.0	41.0
43	36.151373839315376	38.0	35.0	40.0	30.0	41.0
44	36.07724346299255	38.0	35.0	40.0	29.0	41.0
45	35.87609187402123	38.0	35.0	40.0	29.0	41.0
46	35.727701409431006	38.0	34.0	40.0	28.0	41.0
47	35.59840232848759	38.0	34.0	40.0	28.0	41.0
48	35.48402486672889	38.0	34.0	40.0	28.0	41.0
49	35.326810826201815	37.0	34.0	40.0	28.0	41.0
50	35.18880839014822	37.0	34.0	40.0	27.0	41.0
51	33.8939509941946	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	7.0
12	5.0
13	13.0
14	17.0
15	31.0
16	55.0
17	107.0
18	197.0
19	330.0
20	676.0
21	986.0
22	1438.0
23	1969.0
24	2560.0
25	3178.0
26	4183.0
27	5499.0
28	6977.0
29	9186.0
30	11619.0
31	14805.0
32	19483.0
33	26236.0
34	39998.0
35	49643.0
36	59695.0
37	85060.0
38	127925.0
39	160231.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.08372747836816	31.74067102203521	20.671496591106823	26.504104908489808
2	25.4988373380578	29.458373538763304	19.869497128936835	25.173291994242053
3	22.72047075944762	30.135564800607433	19.732192290048562	27.41177214989639
4	22.65292563709129	31.17468402486673	18.208235126627333	27.96415521141465
5	18.80459370106142	34.343768290175106	16.867931094484078	29.983706914279384
6	21.180220510305773	38.46496986570068	17.73984845848427	22.61496116550928
7	84.67548286062294	5.0896119714633725	5.335431924956895	4.8994732429568
8	85.91106822531914	4.57329515794802	4.501479032538716	5.014157584194125
9	77.78176756252275	8.321812170776848	8.512583640476453	5.3838366262239585
10	35.49330085261876	32.33560592878498	13.62703070376639	18.54406251482987
11	26.658335574291726	28.140690004271	24.951516206083806	20.249458215353467
12	27.85041998196688	21.910087476469936	29.98924339971843	20.250249141844755
13	27.124982204153948	23.175411677238717	28.125662400936456	21.57394371767088
14	22.577313064523782	25.74987740639385	28.793520730183335	22.87928879889903
15	21.15206352721578	29.03506968062388	28.203964123574355	21.60890266858598
16	22.669851464004935	25.390954964645584	27.814195548665705	24.124998022683773
17	21.428571428571427	25.103848648306627	28.852365661135455	24.61521426198649
18	23.109448407864974	25.805084075486022	27.14412262524321	23.94134489140579
19	22.6946865558315	29.165730736985306	26.752930382650238	21.38665232453296
20	24.380783649967572	27.317177341537878	25.76632867741272	22.53571033108183
21	24.441052248604013	27.32983216539855	26.230760713099325	21.998354872898112
22	22.05514339497287	25.287659964882863	25.29003274435674	27.367163895787527
23	22.862362971985384	27.244886660233796	24.432193871901546	25.46055649587927
24	22.19102456617682	28.183241849502505	25.239413448914057	24.386320135406617
25	24.095101001312937	26.26635240520746	23.961750794881123	25.67679579859848
26	24.41210433902273	26.85559264121993	24.99153708654318	23.740765933214167
27	22.322160178433016	24.72167296771438	25.551196671781323	27.40497018207128
28	23.231092902225665	27.38298242561336	26.149611655092777	23.236313017068195
29	23.38136893557113	29.44160589714792	23.771770251672812	23.405254915608143
30	25.389847667557774	25.584890140310357	24.33142983691096	24.693832355220906
31	25.243209896072262	27.171172311245396	22.75036778081845	24.835250011863895
32	24.692725058133096	26.660233797870823	24.20314156002341	24.443899583972666
33	23.754686239460902	26.39527342328804	24.952781688469873	24.897258648781182
34	23.034468576490504	27.180030687947866	25.032981634686873	24.752519100874764
35	25.159371687995318	26.70199471661104	24.351361184491513	23.78727241090213
36	25.711438378917062	27.76515810620561	24.641789392093898	21.88161412278343
37	22.875966907635604	30.393248651470333	22.703861303130488	24.026923137763575
38	24.252495373080027	28.2113988325925	25.439834221807423	22.09627157252005
39	24.457661704921144	25.018903143141873	25.288609076672415	25.234826075264564
40	23.973140136355727	25.868516380087637	26.31618077415885	23.842162709397787
41	22.75258237499407	25.302687568217408	28.30409541737191	23.640634639416614
42	22.83452235949191	27.53547305313444	25.298891121059206	24.33111346631444
43	21.849977063131753	25.90315896040622	26.44905642469589	25.797807551766137
44	23.796605343499376	23.463625290665487	25.383520255627438	27.3562491102077
45	24.695414208203488	25.07806444469051	27.23349731875919	22.993024028346806
46	23.09188983975829	25.38083110555705	28.343641741936505	23.183637312748154
47	23.353370137779393	26.477846148978912	25.915813784266888	24.252969928974803
48	22.58965151778794	24.815002293686824	27.96051694955471	24.63482923897053
49	22.37262128857744	25.95836562949839	27.014885236566116	24.654127845358055
50	22.936077320973787	22.681082620181282	27.669930556654066	26.71290950219087
51	23.740765933214167	22.684246326146447	29.06860496385466	24.506382776784726
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1046.0
1	899.0
2	752.0
3	545.5
4	339.0
5	325.0
6	311.0
7	324.0
8	337.0
9	347.5
10	358.0
11	478.5
12	599.0
13	612.5
14	626.0
15	754.5
16	883.0
17	835.0
18	787.0
19	919.5
20	1052.0
21	1306.0
22	1560.0
23	1785.5
24	2011.0
25	2385.5
26	3781.5
27	4803.0
28	5027.5
29	5252.0
30	7353.0
31	9454.0
32	10689.5
33	11925.0
34	12492.0
35	13059.0
36	15710.0
37	18361.0
38	19905.5
39	21450.0
40	22792.0
41	24134.0
42	26337.5
43	28541.0
44	33644.5
45	38748.0
46	46629.0
47	54510.0
48	57988.5
49	61467.0
50	61056.0
51	60645.0
52	54472.5
53	48300.0
54	43317.5
55	38335.0
56	36647.5
57	34960.0
58	32694.5
59	30429.0
60	29333.5
61	28238.0
62	25825.0
63	23412.0
64	20590.0
65	17768.0
66	15776.5
67	13785.0
68	12371.0
69	10957.0
70	8997.0
71	7037.0
72	6263.5
73	5490.0
74	4283.0
75	2518.0
76	1960.0
77	1583.5
78	1207.0
79	991.0
80	775.0
81	566.0
82	357.0
83	261.5
84	166.0
85	113.5
86	61.0
87	44.5
88	28.0
89	25.5
90	23.0
91	25.0
92	27.0
93	15.5
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	632170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.70148572144482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.3850619751162	20.60576732192469
2	10.023431841217558	5.55327908058727
3	3.585591023072095	2.9797859558571704
4	1.8000853690257328	1.9946015658979226
5	1.0966688785598968	1.5189678640289939
6	0.7447692132503012	1.2378728235974945
7	0.5718922513205364	1.1089585523912542
8	0.4603865991758908	1.0202714242732382
9	0.40847329682864336	1.018378547972114
>10	5.826465183297957	39.483762523835466
>50	1.0036880010384805	17.69185894522782
>100	0.08832454409138621	4.310677714533887
>500	0.004014752004153919	0.7829507510402107
>1k	0.001147072001186834	0.6928669288324592
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	3263	0.5161586282170936	TruSeq Adapter, Index 21 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1098	0.1736874574877011	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	854	0.1350902447126564	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	845	0.13366657702833099	No Hit
TACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	842	0.13319202113355585	No Hit
AATCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCT	685	0.1083569293069902	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5993640951010013	0.0
2	0.0	0.0	0.0	0.8076941329072876	0.0
3	0.0	0.0	0.0	1.0076403499058797	0.0
4	0.0	0.0	0.0	2.0910514576775237	0.0
5	0.0	0.0	0.0	2.284828448044039	0.0
6	0.0	0.0	0.0	3.4777037822104813	0.0
7	0.0	0.0	0.0	4.3382318047360675	0.0
8	0.0	0.0	0.0	5.193065156524352	0.0
9	0.0	0.0	0.0	6.262397772751001	0.0
10	0.0	0.0	0.0	7.921445180884889	0.0
11	0.0	0.0	0.0	9.32138507047155	0.0
12	0.0	0.0	0.0	9.979119540629894	0.0
13	0.0	0.0	0.0	10.267965895249695	0.0
14	0.0	0.0	0.0	10.592403941977633	0.0
15	0.0	0.0	0.0	10.876504737649682	0.0
16	0.0	0.0	0.0	11.445180884888558	0.0
17	0.0	0.0	0.0	12.102598984450385	0.0
18	0.0	0.0	0.0	12.963917933467263	0.0
19	0.0	0.0	0.0	13.321258522232943	0.0
20	0.0	0.0	0.0	13.765759210338992	0.0
21	0.0	0.0	0.0	14.21010171314678	0.0
22	0.0	0.0	0.0	14.70506351139725	0.0
23	0.0	0.0	0.0	15.267095876109275	0.0
24	0.0	0.0	0.0	15.65417530094753	0.0
25	0.0	0.0	0.0	16.019108784029612	0.0
26	0.0	0.0	0.0	16.336112121739404	0.0
27	0.0	0.0	0.0	16.70942942562918	0.0
28	0.0	0.0	0.0	17.066611829096605	0.0
29	0.0	0.0	0.0	17.425692456143125	0.0
30	0.0	0.0	0.0	17.926507110429156	0.0
31	0.0	0.0	0.0	18.26897828115855	0.0
32	0.0	0.0	0.0	18.621573310976476	0.0
33	3.163705965167597E-4	0.0	0.0	19.011500071183384	0.0
34	3.163705965167597E-4	0.0	0.0	19.37121343942294	0.0
35	3.163705965167597E-4	0.0	0.0	19.754496417102995	0.0
36	3.163705965167597E-4	0.0	0.0	20.10550959393834	0.0
37	3.163705965167597E-4	0.0	0.0	20.452568138317226	0.0
38	3.163705965167597E-4	0.0	0.0	20.83664204248857	0.0
39	3.163705965167597E-4	0.0	0.0	21.194931743043803	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2107739E-7	45.000004	1
AACCGCG	35	1.2107739E-7	45.000004	1
CTACACG	35	1.2107739E-7	45.000004	1
ATTTACG	25	3.8888065E-5	45.0	1
CTTCGAA	20	7.030875E-4	45.0	33
AACCGTC	20	7.030875E-4	45.0	17
AACGTAG	25	3.8888065E-5	45.0	1
TAATACG	20	7.030875E-4	45.0	1
TCGTTGT	20	7.030875E-4	45.0	11
GGTCGAT	20	7.030875E-4	45.0	9
CGTGCAT	20	7.030875E-4	45.0	18
ATTAACG	20	7.030875E-4	45.0	1
CGTAGAT	20	7.030875E-4	45.0	44
AATTACG	40	6.8066583E-9	45.0	1
CGGTCTA	20	7.030875E-4	45.0	31
ACGTAGA	20	7.030875E-4	45.0	43
ACGTACG	20	7.030875E-4	45.0	1
CGCGTAG	20	7.030875E-4	45.0	1
ACGAACC	20	7.030875E-4	45.0	17
ACGTTCC	30	2.1639462E-6	44.999996	18
>>END_MODULE