FastQCFastQC Report
Sat 18 Jun 2016
SRR3548494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268301
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT27681.0316771089187144Illumina PCR Primer Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14420.5374560661346771No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT12110.4513587351519376RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC12100.4509860194333976No Hit
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT11810.4401772635957376RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT11080.4129690161423178No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT10710.39917853455633784RNA PCR Primer, Index 33 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT5940.2213931368127588RNA PCR Primer, Index 33 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC5270.19642118367057892Illumina PCR Primer Index 5 (96% over 25bp)
AGGGCCGGGATGTTTGGCCTTGAATTTTTTCTGGTGTTAGGAGCCCTTTTG3570.1330595115187793No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT3540.13194136436315929No Hit
AACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG3040.11330557843615939Illumina PCR Primer Index 5 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.11032385268783941No Hit
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT2950.10995113696929942RNA PCR Primer, Index 33 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC2720.10137867544287944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT302.1592605E-645.0000046
GTCGCAG302.1592605E-645.00000419
CAAAACG302.1592605E-645.0000041
ATTACGG302.1592605E-645.0000042
AGGGTTC302.1592605E-645.0000046
ACCTAGG302.1592605E-645.0000042
AACTACG302.1592605E-645.0000041
TGGGACG302.1592605E-645.0000046
GACCTGC302.1592605E-645.0000049
GTAACGG302.1592605E-645.0000042
TCACGCG207.023612E-445.01
GTCATTA253.8827875E-545.043
TCGTTGG207.023612E-445.02
ATCTATT207.023612E-445.029
ATAGGTG207.023612E-445.044
CAAGTCA207.023612E-445.029
AGATATC207.023612E-445.032
TTTACGG253.8827875E-545.02
ACGTTGG207.023612E-445.02
ACTTGCC207.023612E-445.032
Produced by FastQC (version 0.11.5)