##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548494_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268301
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.770235668148832	31.0	31.0	34.0	30.0	34.0
2	31.96967957629677	33.0	31.0	34.0	30.0	34.0
3	32.10595562446655	34.0	31.0	34.0	30.0	34.0
4	35.76377650474654	37.0	35.0	37.0	35.0	37.0
5	35.55586822263055	37.0	35.0	37.0	33.0	37.0
6	35.53649818673803	37.0	35.0	37.0	33.0	37.0
7	35.52388175966545	37.0	35.0	37.0	33.0	37.0
8	35.794704455070985	37.0	35.0	37.0	35.0	37.0
9	37.3474157755655	39.0	37.0	39.0	34.0	39.0
10	36.9590012709606	39.0	37.0	39.0	33.0	39.0
11	37.00072306849397	39.0	37.0	39.0	33.0	39.0
12	36.934722569054905	39.0	37.0	39.0	33.0	39.0
13	36.8731499323521	39.0	37.0	39.0	33.0	39.0
14	37.99228478462622	40.0	37.0	41.0	33.0	41.0
15	37.996843097863966	40.0	37.0	41.0	33.0	41.0
16	37.905684287423455	40.0	37.0	41.0	33.0	41.0
17	37.91703720821018	40.0	37.0	41.0	33.0	41.0
18	37.83939679688112	40.0	37.0	41.0	33.0	41.0
19	37.78608726765834	40.0	36.0	41.0	33.0	41.0
20	37.7415999194934	39.0	36.0	41.0	33.0	41.0
21	37.68166350479499	39.0	36.0	41.0	33.0	41.0
22	37.681864771283	39.0	36.0	41.0	33.0	41.0
23	37.5885069381031	39.0	36.0	41.0	32.0	41.0
24	37.50738908912005	39.0	36.0	41.0	32.0	41.0
25	37.47104557940522	39.0	36.0	41.0	32.0	41.0
26	37.44724768077644	39.0	36.0	41.0	32.0	41.0
27	37.38816478507348	39.0	36.0	41.0	32.0	41.0
28	37.111646993488655	39.0	36.0	41.0	31.0	41.0
29	36.94828942120976	39.0	35.0	41.0	31.0	41.0
30	36.85510676441758	39.0	35.0	41.0	31.0	41.0
31	36.62245761290491	39.0	35.0	40.0	30.0	41.0
32	36.7062180163324	39.0	35.0	40.0	30.0	41.0
33	36.46447087413017	39.0	35.0	40.0	30.0	41.0
34	36.48380736560803	39.0	35.0	40.0	30.0	41.0
35	36.398064114557904	39.0	35.0	40.0	30.0	41.0
36	36.2787950846251	39.0	35.0	40.0	30.0	41.0
37	36.152392275839446	38.0	35.0	40.0	30.0	41.0
38	36.128426655137325	38.0	35.0	40.0	30.0	41.0
39	36.09108426729681	38.0	35.0	40.0	30.0	41.0
40	36.058013201590754	38.0	35.0	40.0	29.0	41.0
41	36.08673094770426	38.0	35.0	40.0	30.0	41.0
42	36.004319775177876	38.0	35.0	40.0	29.0	41.0
43	35.91655640493327	38.0	35.0	40.0	29.0	41.0
44	35.77812978706751	38.0	35.0	40.0	29.0	41.0
45	35.51223066630389	38.0	34.0	40.0	27.0	41.0
46	35.38432581317252	38.0	34.0	40.0	27.0	41.0
47	35.31808677567359	38.0	34.0	40.0	27.0	41.0
48	35.325131102754	38.0	34.0	40.0	27.0	41.0
49	35.13163573747396	38.0	34.0	40.0	26.0	41.0
50	34.92730925341314	38.0	34.0	40.0	26.0	41.0
51	33.62992310874726	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	2.0
14	5.0
15	7.0
16	16.0
17	47.0
18	126.0
19	237.0
20	433.0
21	635.0
22	924.0
23	1212.0
24	1522.0
25	1751.0
26	2108.0
27	2583.0
28	3204.0
29	4029.0
30	5247.0
31	6709.0
32	9099.0
33	11927.0
34	17545.0
35	22043.0
36	25536.0
37	36636.0
38	54789.0
39	59914.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.832945087793185	34.22462085493532	18.92985862892796	26.01257542834354
2	21.237341642409085	34.26040156391515	19.737160875285593	24.765095918390166
3	20.12590336972281	32.22686460356093	21.619747969631124	26.027484057085136
4	21.064401549006526	32.09417780776069	20.10987659382559	26.731544049407198
5	17.475521895184887	34.99949683377997	18.781890488667578	28.743090782367563
6	20.19373763049709	37.02073417542238	19.967871905061852	22.817656289018675
7	80.53007629490759	6.604149816810224	7.147196618723001	5.718577269559189
8	82.30606669375068	4.995881491310133	5.378287818532171	7.319763996407021
9	74.37989422327908	9.41517176603889	10.971259890943381	5.233674119738652
10	28.26042392685827	41.457169373203975	13.17214620892207	17.110260491015687
11	22.005881454038562	28.55076947160093	31.43484370166343	18.008505372697083
12	24.48145925658123	22.584708964931178	34.0986429420688	18.835188836418798
13	27.69091430892915	24.715897443542886	27.509401754000173	20.083786493527793
14	19.416252641622656	29.34577209924674	31.47509699926575	19.762878259864852
15	18.045031513114	29.015918688338843	31.581693694768187	21.357356103778965
16	19.474396293714896	30.888069742565254	27.60518969366495	22.032344270054903
17	18.9078684015341	27.185511794588912	29.11133391228508	24.795285891591906
18	21.6503106585514	25.863116425209	27.69203245608477	24.794540460154828
19	21.505696959757884	31.08150920048751	28.95479331049828	18.45800052925632
20	21.99395455104528	28.643575685517387	27.57276342615197	21.78970633728536
21	21.455753053473526	30.205627261918515	26.736762069466756	21.601857615141203
22	19.936191068985952	29.059526427408024	26.692036183241957	24.31224632036407
23	21.198579207680925	27.992068609509467	25.378958706825543	25.430393475984065
24	16.455771689259453	30.283152131374834	27.088605707768515	26.172470471597197
25	19.040182481615798	29.60667310222474	26.28763962862606	25.065504787533406
26	22.933943593203157	28.05393941878711	26.031583929989075	22.980533058020654
27	19.264930059895416	28.46690843492943	24.812058098926208	27.45610340624895
28	21.759516364083623	28.94435727037916	27.898516964155927	21.397609401381285
29	24.917536647273025	27.39162358694153	25.002515831100148	22.688323934685297
30	25.2332268608764	29.192213223208263	23.473635953649076	22.10092396226626
31	23.78373543147435	29.59772792497978	24.521712554183548	22.096824089362322
32	26.824350263323655	26.977163707925055	23.65514850857805	22.54333752017324
33	22.56942762047104	28.462808562025486	27.444176503255672	21.523587314247806
34	23.304050301713374	26.48406081229664	28.381929251102306	21.829959634887683
35	24.961144386342205	27.631279793962747	24.900764439938726	22.50681137975632
36	24.936545148918565	26.959273353435137	30.08002206477054	18.024159432875763
37	23.909713344340872	28.312976843172404	25.4661741849639	22.31113562752282
38	24.19595901617959	27.912307445741906	28.24402443524251	19.647709102835993
39	23.279451064289734	24.911573195776384	27.460575994871434	24.348399745062448
40	23.814670836113173	27.655879031386394	25.54593534873146	22.983514783768978
41	20.79716437881335	23.336849284944893	30.902978371306855	24.963007964934906
42	24.911200480057847	26.619356618126655	25.424802740205966	23.044640161609536
43	22.53811950011368	23.93207628745327	27.535119138579432	25.994685073853617
44	24.31224632036407	22.899653747097474	26.413244825774036	26.374855106764418
45	25.1270028810925	23.72000104360401	28.724454996440564	22.428541078862917
46	21.536259648678165	24.857902132306624	30.205627261918515	23.400210957096693
47	21.782997454351644	25.74459282671328	27.152712811357393	25.319696907577683
48	21.80796940749382	22.658506677202098	32.448257740373684	23.085266174930396
49	22.34207103216164	21.648447079958704	28.731909310811364	27.277572577068295
50	20.54707213167301	21.78560646438142	29.425905978732843	28.24141542521273
51	21.696154691931824	20.53253621864995	31.685681380240847	26.085627709177377
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1688.0
1	1355.5
2	1023.0
3	705.5
4	388.0
5	382.5
6	377.0
7	378.0
8	379.0
9	452.0
10	525.0
11	568.5
12	612.0
13	660.5
14	709.0
15	674.0
16	639.0
17	656.5
18	674.0
19	669.5
20	665.0
21	756.0
22	847.0
23	909.0
24	971.0
25	1182.0
26	1899.5
27	2406.0
28	2661.0
29	2916.0
30	3602.0
31	4288.0
32	4406.0
33	4524.0
34	4996.5
35	5469.0
36	6243.0
37	7017.0
38	7912.0
39	8807.0
40	9494.5
41	10182.0
42	12650.0
43	15118.0
44	18651.0
45	22184.0
46	24561.5
47	26939.0
48	29750.5
49	32562.0
50	29768.0
51	26974.0
52	23970.0
53	20966.0
54	18326.5
55	15687.0
56	14228.5
57	12770.0
58	11729.5
59	10689.0
60	9399.5
61	8110.0
62	7531.5
63	6953.0
64	6060.5
65	5168.0
66	4025.0
67	2882.0
68	2189.5
69	1497.0
70	1366.5
71	1236.0
72	980.0
73	724.0
74	701.0
75	509.5
76	341.0
77	241.5
78	142.0
79	125.0
80	108.0
81	69.5
82	31.0
83	22.5
84	14.0
85	11.5
86	9.0
87	8.5
88	8.0
89	5.5
90	3.0
91	5.0
92	7.0
93	4.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	268301.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.60404545640903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00311278179292	26.646192149861538
2	10.056762491517938	6.960093328015922
3	3.659942052712644	3.799464034796739
4	1.791195889835529	2.4793049597280667
5	1.011384810917355	1.7499002985452907
6	0.6645627564813718	1.3797935900350726
7	0.5029996876447336	1.2184076839072533
8	0.4071389334683283	1.1270923328649538
9	0.29296769815710394	0.912408078985915
>10	3.9205971371024204	31.98832654369533
>50	0.6182480100815355	13.914968635972286
>100	0.06139396615792252	3.6824312991751804
>500	0.0021541742511551757	0.4178143204833377
>1k	0.007539609879043115	3.7238027439331196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2768	1.0316771089187144	Illumina PCR Primer Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1442	0.5374560661346771	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1211	0.4513587351519376	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	1210	0.4509860194333976	No Hit
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1181	0.4401772635957376	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	1108	0.4129690161423178	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	1071	0.39917853455633784	RNA PCR Primer, Index 33 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	594	0.2213931368127588	RNA PCR Primer, Index 33 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	527	0.19642118367057892	Illumina PCR Primer Index 5 (96% over 25bp)
AGGGCCGGGATGTTTGGCCTTGAATTTTTTCTGGTGTTAGGAGCCCTTTTG	357	0.1330595115187793	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	354	0.13194136436315929	No Hit
AACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	304	0.11330557843615939	Illumina PCR Primer Index 5 (95% over 24bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	296	0.11032385268783941	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	295	0.10995113696929942	RNA PCR Primer, Index 33 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	272	0.10137867544287944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1584004532223138	0.0
2	0.0	0.0	0.0	1.6194497970562913	0.0
3	0.0	0.0	0.0	2.1282067528633886	0.0
4	0.0	0.0	0.0	4.760325157192854	0.0
5	0.0	0.0	0.0	5.236283129768432	0.0
6	0.0	0.0	0.0	7.573210685014219	0.0
7	0.0	0.0	0.0	9.23924994688801	0.0
8	0.0	0.0	0.0	10.610843791115203	0.0
9	0.0	0.0	0.0	12.483740276778693	0.0
10	0.0	0.0	0.0	15.245936466878618	0.0
11	0.0	0.0	0.0	17.657034450113866	0.0
12	0.0	0.0	0.0	18.871342261117178	0.0
13	0.0	0.0	0.0	19.426315966023235	0.0
14	0.0	0.0	0.0	19.97793522946243	0.0
15	0.0	0.0	0.0	20.45389320203801	0.0
16	0.0	0.0	0.0	21.404691000033544	0.0
17	0.0	0.0	0.0	22.57352749337498	0.0
18	0.0	0.0	0.0	24.472514079336268	0.0
19	0.0	0.0	0.0	25.074077249059826	0.0
20	0.0	0.0	0.0	25.81130894033194	0.0
21	0.0	0.0	0.0	26.453498123376356	0.0
22	0.0	0.0	0.0	27.166503292943375	0.0
23	0.0	0.0	0.0	27.79266570009057	0.0
24	0.0	0.0	0.0	28.261914789732426	0.0
25	0.0	0.0	0.0	28.665193197192707	0.0
26	0.0	0.0	0.0	29.05579927022262	0.0
27	0.0	0.0	0.0	29.60667310222474	0.0
28	0.0	0.0	0.0	30.05244110159858	0.0
29	0.0	0.0	0.0	30.486654913697674	0.0
30	0.0	0.0	0.0	31.19034219030119	0.0
31	0.0	0.0	0.0	31.59883861782103	0.0
32	0.0	0.0	0.0	32.0263435469864	0.0
33	0.0	0.0	0.0	32.44676687749953	0.0
34	0.0	0.0	0.0	32.86644477657556	0.0
35	0.0	0.0	0.0	33.29432242145948	0.0
36	0.0	0.0	0.0	33.68008319014838	0.0
37	0.0	0.0	0.0	34.08075258757888	0.0
38	0.0	0.0	0.0	34.506393938151554	0.0
39	0.0	0.0	0.0	34.91899023857533	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGT	30	2.1592605E-6	45.000004	6
GTCGCAG	30	2.1592605E-6	45.000004	19
CAAAACG	30	2.1592605E-6	45.000004	1
ATTACGG	30	2.1592605E-6	45.000004	2
AGGGTTC	30	2.1592605E-6	45.000004	6
ACCTAGG	30	2.1592605E-6	45.000004	2
AACTACG	30	2.1592605E-6	45.000004	1
TGGGACG	30	2.1592605E-6	45.000004	6
GACCTGC	30	2.1592605E-6	45.000004	9
GTAACGG	30	2.1592605E-6	45.000004	2
TCACGCG	20	7.023612E-4	45.0	1
GTCATTA	25	3.8827875E-5	45.0	43
TCGTTGG	20	7.023612E-4	45.0	2
ATCTATT	20	7.023612E-4	45.0	29
ATAGGTG	20	7.023612E-4	45.0	44
CAAGTCA	20	7.023612E-4	45.0	29
AGATATC	20	7.023612E-4	45.0	32
TTTACGG	25	3.8827875E-5	45.0	2
ACGTTGG	20	7.023612E-4	45.0	2
ACTTGCC	20	7.023612E-4	45.0	32
>>END_MODULE