SRR3548493_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269819
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2820	1.0451450787379688	Illumina PCR Primer Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1446	0.5359148169698946	No Hit
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1222	0.4528962007864531	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	1212	0.44919001256397806	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1163	0.4310296902738503	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	1090	0.40397451624978226	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	1052	0.389891001004377	RNA PCR Primer, Index 33 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	661	0.2449790415056019	RNA PCR Primer, Index 33 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	586	0.2171826298370389	Illumina PCR Primer Index 5 (96% over 25bp)
GAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	400	0.14824752889900267	No Hit
AGGGCCGGGATGTTTGGCCTTGAATTTTTTCTGGTGTTAGGAGCCCTTTTG	350	0.1297165877866273	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	348	0.12897535014213232	RNA PCR Primer, Index 33 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	334	0.12378668663066722	No Hit
GAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	282	0.10451450787379687	RNA PCR Primer, Index 33 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	275	0.10192017611806432	No Hit
AACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	274	0.10154955729581683	Illumina PCR Primer Index 5 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	271	0.1004377008290743	RNA PCR Primer, Index 33 (96% over 26bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	60	0.0	45.000004	2
ATTAGTC	25	3.882845E-5	45.000004	42
CTCGTAC	25	3.882845E-5	45.000004	29
CGTATAG	30	2.159306E-6	45.000004	1
GCGGTTA	30	2.159306E-6	45.000004	30
TCACACG	30	2.159306E-6	45.000004	1
CAGCACG	25	3.882845E-5	45.000004	1
TGAACGG	25	3.882845E-5	45.000004	2
CGTTCCC	25	3.882845E-5	45.000004	44
ACTACGG	50	2.1827873E-11	45.000004	2
CAAGCCA	25	3.882845E-5	45.000004	17
AAGGGTA	25	3.882845E-5	45.000004	5
CAGTCGT	25	3.882845E-5	45.000004	37
ATAGTAC	25	3.882845E-5	45.000004	14
ATACGCG	30	2.159306E-6	45.000004	1
CGTCCGG	25	3.882845E-5	45.000004	2
GATTATC	30	2.159306E-6	45.000004	9
CTCTAGG	30	2.159306E-6	45.000004	2
GTGCGAT	25	3.882845E-5	45.000004	14
CACCCTA	25	3.882845E-5	45.000004	27

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content