##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269819
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.753764560686978	31.0	31.0	34.0	30.0	34.0
2	31.935560505375825	33.0	31.0	34.0	30.0	34.0
3	32.08947850225522	34.0	31.0	34.0	30.0	34.0
4	35.70490958753831	37.0	35.0	37.0	33.0	37.0
5	35.522187095793846	37.0	35.0	37.0	33.0	37.0
6	35.52196843068872	37.0	35.0	37.0	33.0	37.0
7	35.55216274613723	37.0	35.0	37.0	35.0	37.0
8	35.805528891590285	37.0	35.0	37.0	35.0	37.0
9	37.335788065332686	39.0	37.0	39.0	34.0	39.0
10	36.94388089793528	39.0	37.0	39.0	33.0	39.0
11	36.99569340928549	39.0	37.0	39.0	33.0	39.0
12	36.94908068001141	39.0	37.0	39.0	33.0	39.0
13	36.86572109451151	39.0	37.0	39.0	33.0	39.0
14	37.98178779107476	40.0	37.0	41.0	33.0	41.0
15	37.9893150593546	40.0	37.0	41.0	33.0	41.0
16	37.915120877328874	40.0	37.0	41.0	33.0	41.0
17	37.90480655550573	40.0	37.0	41.0	33.0	41.0
18	37.80857167212094	39.0	37.0	41.0	33.0	41.0
19	37.756410779077825	40.0	36.0	41.0	33.0	41.0
20	37.69630752467395	39.0	36.0	41.0	33.0	41.0
21	37.66898179890964	39.0	36.0	41.0	33.0	41.0
22	37.6508029456784	39.0	36.0	41.0	33.0	41.0
23	37.56489720886965	39.0	36.0	41.0	32.0	41.0
24	37.508470493182465	39.0	36.0	41.0	32.0	41.0
25	37.470630311430995	39.0	36.0	41.0	32.0	41.0
26	37.43952797986798	39.0	36.0	41.0	32.0	41.0
27	37.36414781761106	39.0	36.0	41.0	32.0	41.0
28	37.10815398470827	39.0	36.0	41.0	31.0	41.0
29	36.92491262661265	39.0	35.0	41.0	31.0	41.0
30	36.84986972748398	39.0	35.0	40.0	31.0	41.0
31	36.63459949076974	39.0	35.0	40.0	30.0	41.0
32	36.712488742453274	39.0	35.0	40.0	30.0	41.0
33	36.45332611862026	39.0	35.0	40.0	30.0	41.0
34	36.4509912200401	39.0	35.0	40.0	30.0	41.0
35	36.36580448374651	39.0	35.0	40.0	30.0	41.0
36	36.28651429291488	39.0	35.0	40.0	30.0	41.0
37	36.110522238982426	38.0	35.0	40.0	30.0	41.0
38	36.064999129045766	38.0	35.0	40.0	30.0	41.0
39	36.05359148169699	38.0	35.0	40.0	30.0	41.0
40	36.03666902627317	38.0	35.0	40.0	29.0	41.0
41	36.083919219921505	38.0	35.0	40.0	30.0	41.0
42	35.986138856047944	38.0	35.0	40.0	29.0	41.0
43	35.9057256901849	38.0	35.0	40.0	29.0	41.0
44	35.84115277278472	38.0	35.0	40.0	29.0	41.0
45	35.56068697904892	38.0	34.0	40.0	28.0	41.0
46	35.427293852545596	38.0	34.0	40.0	27.0	41.0
47	35.30853646333283	38.0	34.0	40.0	27.0	41.0
48	35.33238207835623	38.0	34.0	40.0	27.0	41.0
49	35.15463329120633	38.0	34.0	40.0	26.0	41.0
50	34.94067133893462	38.0	34.0	40.0	26.0	41.0
51	33.6779952486667	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	0.0
12	0.0
13	4.0
14	1.0
15	12.0
16	18.0
17	50.0
18	122.0
19	250.0
20	429.0
21	624.0
22	879.0
23	1178.0
24	1492.0
25	1808.0
26	2122.0
27	2599.0
28	3152.0
29	4182.0
30	5203.0
31	7083.0
32	9080.0
33	12159.0
34	17492.0
35	22357.0
36	25876.0
37	36940.0
38	55088.0
39	59599.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.757618996438353	34.26185702267075	19.05759045878904	25.922933522101854
2	21.324295175654793	34.26741630500447	19.746941468169403	24.66134705117134
3	20.13127318684007	31.9139867837328	21.748653727128186	26.20608630229895
4	21.33319002738873	32.262368476645456	19.905936942913584	26.498504553052232
5	17.44799291376812	35.19248088533424	18.76480158921351	28.59472461168413
6	20.152398459708174	37.10746833988711	19.84515545606499	22.894977744339723
7	80.45245145819976	6.654831572276229	7.178515968111957	5.714201001412058
8	82.41747245375603	4.893650928956078	5.393615720167964	7.29526089711992
9	74.37615586745189	9.337741226525932	10.989218698460821	5.296884207561365
10	28.275992424551273	41.25432234201446	13.217749676635076	17.25193555679919
11	22.074057053061498	28.38643683358103	31.4974112275266	18.042094885830874
12	24.627991357169066	22.48803827751196	33.92644698853676	18.957523376782213
13	27.345739180710034	24.72991153328713	27.533642923589518	20.390706362413322
14	19.26847256864787	29.149911607410893	31.679755688072376	19.90186013586886
15	18.033570652919177	28.944218161063528	31.654553608159542	21.36765757785775
16	19.54495421004451	30.85253447681594	27.582935226948436	22.019576086191115
17	18.911566642823523	27.143010684940645	29.29185861633169	24.65356405590414
18	21.575204118316353	25.809894781316363	27.709686864157085	24.9052142362102
19	21.539254092558345	31.087136191298608	28.82636137558882	18.547248340554223
20	21.968801307543202	28.714434491270076	27.489168664919816	21.827595536266905
21	21.359874582590553	30.224335573106416	26.729029460490178	21.686760383812853
22	19.941516349849344	28.962378483353657	26.71568718288927	24.380417983907734
23	21.173453315000053	27.9150096916822	25.4778203165826	25.433716676735145
24	16.435832910210177	30.193574210859875	27.19007927536608	26.18051360356387
25	19.095393578658285	29.510894340279965	26.327278657173885	25.06643342388787
26	22.935375195964703	28.017300486622513	26.14938162249508	22.897942694917703
27	19.303681356761384	28.57878800232749	24.76993836608986	27.34759227482127
28	21.797946030487104	28.86750006485829	27.749343078137567	21.585210826517034
29	25.06643342388787	27.530307354189286	24.75177804379973	22.651481178123113
30	25.091264884978447	29.06503989711621	23.669200464014764	22.17449475389057
31	23.853768637494024	29.64542897275581	24.585370192610604	21.915432197139566
32	27.10928437211612	26.74496606984682	23.64325714645744	22.502492411579613
33	22.567721324295174	28.454630696874574	27.41245056871458	21.56519741011567
34	23.294504834722538	26.64378713137325	28.378653838313834	21.683054195590376
35	25.035301442819076	27.562551191724822	24.965625104236544	22.436522261219558
36	24.953394683102374	26.92545743628136	30.044214825494127	18.076933055122137
37	23.835978934026144	28.208539798902226	25.614949280814177	22.340531986257453
38	24.108013149555813	28.036202046557136	28.149982024987118	19.70580277889993
39	23.254478001919807	24.70322697808531	27.64371671379703	24.39857830619786
40	23.86340472687246	27.587753271637656	25.545643561053893	23.003198440435995
41	20.693131321367286	23.317853820524128	31.028207798561258	24.960807059547328
42	24.827384283538223	26.542608192899685	25.561209551588288	23.068797971973805
43	22.57216875016215	23.969772328857495	27.618514633884196	25.839544287096167
44	24.357068998106136	22.6607466486793	26.6441577501955	26.33802660301906
45	25.180213402317854	23.813741804691293	28.58990656699491	22.416138225995947
46	21.58039278182782	24.815153862404056	30.236195375418333	23.368257980349792
47	21.689725334390833	25.828796341250985	27.211945785878683	25.2695325384795
48	21.888006404293247	22.50731045626883	32.57257643086662	23.032106708571302
49	22.37722324965996	21.561861840715444	28.70479840189164	27.356116507732963
50	20.64383901800837	21.753471771817402	29.205875049570267	28.396814160603963
51	21.622272708741786	20.361427475455766	32.00923582105041	26.007063994752038
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1729.0
1	1367.5
2	1006.0
3	694.5
4	383.0
5	360.5
6	338.0
7	364.5
8	391.0
9	453.0
10	515.0
11	546.5
12	578.0
13	609.0
14	640.0
15	642.5
16	645.0
17	638.5
18	632.0
19	679.0
20	726.0
21	800.5
22	875.0
23	949.0
24	1023.0
25	1187.5
26	1887.0
27	2422.0
28	2723.5
29	3025.0
30	3572.5
31	4120.0
32	4346.5
33	4573.0
34	4989.5
35	5406.0
36	6365.5
37	7325.0
38	8092.0
39	8859.0
40	9719.5
41	10580.0
42	12781.0
43	14982.0
44	18660.5
45	22339.0
46	24627.0
47	26915.0
48	29729.0
49	32543.0
50	29830.0
51	27117.0
52	24117.0
53	21117.0
54	18684.0
55	16251.0
56	14522.5
57	12794.0
58	11711.0
59	10628.0
60	9362.5
61	8097.0
62	7574.5
63	7052.0
64	6061.0
65	5070.0
66	4007.0
67	2944.0
68	2195.5
69	1447.0
70	1330.5
71	1214.0
72	998.5
73	783.0
74	720.0
75	515.5
76	374.0
77	255.5
78	137.0
79	134.5
80	132.0
81	80.0
82	28.0
83	21.0
84	14.0
85	13.0
86	12.0
87	10.5
88	9.0
89	9.0
90	9.0
91	7.5
92	6.0
93	5.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	269819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.22442452162375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91999480204453	26.325425563062645
2	10.072121632158018	6.894251331448118
3	3.6309884778653734	3.7280547329876694
4	1.7900459152733257	2.450531652700514
5	1.017932946374426	1.7419084645632812
6	0.6562418781945768	1.3475700376919342
7	0.508966473187213	1.219335925194297
8	0.4180022524473707	1.1444709231003005
9	0.2967166247942476	0.9139460156623515
>10	3.9699384908602613	31.968838369425427
>50	0.6475786190764966	14.397058769026644
>100	0.061725721216321576	3.698405227207869
>500	0.0021658147795200556	0.46216167134264086
>1k	0.007580351728320194	3.708041316586304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2820	1.0451450787379688	Illumina PCR Primer Index 5 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1446	0.5359148169698946	No Hit
AAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1222	0.4528962007864531	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	1212	0.44919001256397806	No Hit
AATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	1163	0.4310296902738503	RNA PCR Primer, Index 33 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGT	1090	0.40397451624978226	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	1052	0.389891001004377	RNA PCR Primer, Index 33 (95% over 23bp)
AAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	661	0.2449790415056019	RNA PCR Primer, Index 33 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	586	0.2171826298370389	Illumina PCR Primer Index 5 (96% over 25bp)
GAAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	400	0.14824752889900267	No Hit
AGGGCCGGGATGTTTGGCCTTGAATTTTTTCTGGTGTTAGGAGCCCTTTTG	350	0.1297165877866273	No Hit
AATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	348	0.12897535014213232	RNA PCR Primer, Index 33 (95% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	334	0.12378668663066722	No Hit
GAAAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	282	0.10451450787379687	RNA PCR Primer, Index 33 (95% over 23bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCTC	275	0.10192017611806432	No Hit
AACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	274	0.10154955729581683	Illumina PCR Primer Index 5 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	271	0.1004377008290743	RNA PCR Primer, Index 33 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.412376444950133E-4	0.0	0.0	1.1585544383457058	0.0
2	7.412376444950133E-4	0.0	0.0	1.6455475707789295	0.0
3	7.412376444950133E-4	0.0	0.0	2.146253599635311	0.0
4	7.412376444950133E-4	0.0	0.0	4.74614463770157	0.0
5	7.412376444950133E-4	0.0	0.0	5.224613537223101	0.0
6	7.412376444950133E-4	0.0	0.0	7.599909569007371	0.0
7	7.412376444950133E-4	0.0	0.0	9.313280384257595	0.0
8	7.412376444950133E-4	0.0	0.0	10.669004036038974	0.0
9	7.412376444950133E-4	0.0	0.0	12.609934808149166	0.0
10	7.412376444950133E-4	0.0	0.0	15.429602807808198	0.0
11	7.412376444950133E-4	0.0	0.0	17.848261241795427	0.0
12	7.412376444950133E-4	0.0	0.0	19.049436844699596	0.0
13	7.412376444950133E-4	0.0	0.0	19.589057849891965	0.0
14	7.412376444950133E-4	0.0	0.0	20.150915984419186	0.0
15	7.412376444950133E-4	0.0	0.0	20.60158847227215	0.0
16	7.412376444950133E-4	0.0	0.0	21.532212334935643	0.0
17	7.412376444950133E-4	0.0	0.0	22.68298377801415	0.0
18	7.412376444950133E-4	0.0	0.0	24.561279969164513	0.0
19	7.412376444950133E-4	0.0	0.0	25.134997906003655	0.0
20	7.412376444950133E-4	0.0	0.0	25.89661958572228	0.0
21	7.412376444950133E-4	0.0	0.0	26.532601484699	0.0
22	7.412376444950133E-4	0.0	0.0	27.214910736456662	0.0
23	7.412376444950133E-4	0.0	0.0	27.841997783699444	0.0
24	7.412376444950133E-4	0.0	0.0	28.29304089037466	0.0
25	7.412376444950133E-4	0.0	0.0	28.68256127255679	0.0
26	7.412376444950133E-4	0.0	0.0	29.081717744117352	0.0
27	7.412376444950133E-4	0.0	0.0	29.619856274020734	0.0
28	7.412376444950133E-4	0.0	0.0	30.06793443011797	0.0
29	7.412376444950133E-4	0.0	0.0	30.487104318079897	0.0
30	7.412376444950133E-4	0.0	0.0	31.17608470863801	0.0
31	7.412376444950133E-4	0.0	0.0	31.585618507221508	0.0
32	7.412376444950133E-4	0.0	0.0	32.02665490569604	0.0
33	7.412376444950133E-4	0.0	0.0	32.443601080724484	0.0
34	7.412376444950133E-4	0.0	0.0	32.88278438508778	0.0
35	7.412376444950133E-4	0.0	0.0	33.321597070628826	0.0
36	7.412376444950133E-4	0.0	0.0	33.71556487867793	0.0
37	7.412376444950133E-4	0.0	0.0	34.09433731501488	0.0
38	7.412376444950133E-4	0.0	0.0	34.511283490043326	0.0
39	7.412376444950133E-4	0.0	0.0	34.93786575445021	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	60	0.0	45.000004	2
ATTAGTC	25	3.882845E-5	45.000004	42
CTCGTAC	25	3.882845E-5	45.000004	29
CGTATAG	30	2.159306E-6	45.000004	1
GCGGTTA	30	2.159306E-6	45.000004	30
TCACACG	30	2.159306E-6	45.000004	1
CAGCACG	25	3.882845E-5	45.000004	1
TGAACGG	25	3.882845E-5	45.000004	2
CGTTCCC	25	3.882845E-5	45.000004	44
ACTACGG	50	2.1827873E-11	45.000004	2
CAAGCCA	25	3.882845E-5	45.000004	17
AAGGGTA	25	3.882845E-5	45.000004	5
CAGTCGT	25	3.882845E-5	45.000004	37
ATAGTAC	25	3.882845E-5	45.000004	14
ATACGCG	30	2.159306E-6	45.000004	1
CGTCCGG	25	3.882845E-5	45.000004	2
GATTATC	30	2.159306E-6	45.000004	9
CTCTAGG	30	2.159306E-6	45.000004	2
GTGCGAT	25	3.882845E-5	45.000004	14
CACCCTA	25	3.882845E-5	45.000004	27
>>END_MODULE