FastQCFastQC Report
Sat 18 Jun 2016
SRR3548492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460479
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGCT20040.4351989993028998Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7410.16091939046080278No Hit
AATCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCT6490.14094019488402296No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCC5610.12182965998449441No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGT5570.1209609993072431No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA5540.12030950379930463No Hit
CCTGTCTCTTATACACATCTGACGCAAATGCGGTCGTATGCCGTCTTCTGC5020.10901691499503778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGG900.045.0000042
CGTACAG453.8380676E-1045.0000041
CGTAAGG453.8380676E-1045.0000042
CTTGTCG253.8871505E-545.037
CAGCGCG406.7993824E-945.01
CTCACCG207.028879E-445.01
ATCTACG207.028879E-445.01
CTCCGAT207.028879E-445.011
CGGTTTT207.028879E-445.022
CATTGCG253.8871505E-545.01
CGTTGAG207.028879E-445.01
TTGTCGT253.8871505E-545.038
TGAACGA253.8871505E-545.034
TGAACCG207.028879E-445.01
TCCTCCG207.028879E-445.021
CGACATG253.8871505E-545.01
AAGCGCG406.7993824E-945.01
CGCTGCG253.8871505E-545.01
CTGGTCG207.028879E-445.013
TAGCACG406.7993824E-945.01