##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	771711
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96366515444253	33.0	31.0	34.0	30.0	34.0
2	32.166671202043254	34.0	31.0	34.0	30.0	34.0
3	32.29126706759396	34.0	31.0	34.0	30.0	34.0
4	35.87907908530525	37.0	35.0	37.0	35.0	37.0
5	35.68313397113686	37.0	35.0	37.0	33.0	37.0
6	35.6759279056538	37.0	35.0	37.0	33.0	37.0
7	35.69353423755784	37.0	35.0	37.0	35.0	37.0
8	35.903136018535434	37.0	35.0	37.0	35.0	37.0
9	37.544663740700855	39.0	37.0	39.0	35.0	39.0
10	37.109461961796576	39.0	37.0	39.0	33.0	39.0
11	37.09072956067751	39.0	37.0	39.0	33.0	39.0
12	37.01718518979255	39.0	37.0	39.0	33.0	39.0
13	37.01872721783155	39.0	37.0	39.0	33.0	39.0
14	38.22280879759392	40.0	38.0	41.0	33.0	41.0
15	38.233304954834125	40.0	37.0	41.0	33.0	41.0
16	38.10861319846419	40.0	37.0	41.0	33.0	41.0
17	38.129617175341544	40.0	37.0	41.0	33.0	41.0
18	38.066029899794096	40.0	37.0	41.0	33.0	41.0
19	38.057538379004576	40.0	37.0	41.0	33.0	41.0
20	38.060561531454134	40.0	37.0	41.0	33.0	41.0
21	38.047671991198776	40.0	37.0	41.0	33.0	41.0
22	38.034623064852	40.0	37.0	41.0	33.0	41.0
23	37.91435135691988	40.0	37.0	41.0	33.0	41.0
24	37.79070532880832	40.0	37.0	41.0	33.0	41.0
25	37.80889218891528	40.0	37.0	41.0	33.0	41.0
26	37.74776438329893	40.0	37.0	41.0	33.0	41.0
27	37.68337628982871	40.0	37.0	41.0	33.0	41.0
28	37.65767107116524	40.0	37.0	41.0	33.0	41.0
29	37.534250516061064	40.0	36.0	41.0	32.0	41.0
30	37.50536146303474	40.0	36.0	41.0	32.0	41.0
31	37.43708979138564	40.0	36.0	41.0	32.0	41.0
32	37.39326250370929	40.0	36.0	41.0	32.0	41.0
33	37.363456008790855	40.0	36.0	41.0	32.0	41.0
34	37.26335247262252	39.0	36.0	41.0	31.0	41.0
35	37.21576989313357	39.0	36.0	41.0	31.0	41.0
36	37.1503270006518	39.0	36.0	41.0	31.0	41.0
37	37.06095805294987	39.0	36.0	41.0	31.0	41.0
38	37.069142463953476	39.0	35.0	41.0	31.0	41.0
39	36.98923431180844	39.0	35.0	41.0	31.0	41.0
40	36.870726217457054	39.0	35.0	41.0	31.0	41.0
41	36.817440725867584	39.0	35.0	41.0	31.0	41.0
42	36.7585715377907	39.0	35.0	41.0	31.0	41.0
43	36.71794752180544	39.0	35.0	40.0	31.0	41.0
44	36.57216496849209	39.0	35.0	40.0	30.0	41.0
45	36.40009666831236	39.0	35.0	40.0	30.0	41.0
46	36.2306368575801	38.0	35.0	40.0	30.0	41.0
47	36.14845453803302	38.0	35.0	40.0	29.0	41.0
48	36.12150273871955	38.0	35.0	40.0	29.0	41.0
49	35.967158690235074	38.0	35.0	40.0	29.0	41.0
50	35.82818049762152	38.0	34.0	40.0	29.0	41.0
51	34.61258813208572	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	3.0
11	5.0
12	3.0
13	7.0
14	12.0
15	31.0
16	54.0
17	88.0
18	187.0
19	417.0
20	704.0
21	1129.0
22	1652.0
23	2186.0
24	2779.0
25	3474.0
26	4407.0
27	5717.0
28	7607.0
29	9804.0
30	12850.0
31	16942.0
32	22178.0
33	29713.0
34	44358.0
35	55629.0
36	67817.0
37	97897.0
38	159545.0
39	224439.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.447810125811348	33.58627776460359	19.35037857436268	26.61553353522238
2	23.47433171226016	31.378715607267488	19.790828431887068	25.35612424858529
3	21.493279219811562	29.37900327972518	22.671829221042593	26.455888279420662
4	22.301872073872214	30.569215677889776	18.58338160269842	28.545530645539586
5	18.55202271316594	34.91579101503024	17.319048192911595	29.213138078892225
6	20.99944150076907	35.167957953171594	21.51245738365787	22.320143162401468
7	84.75841344752115	5.472644552170436	5.339822809315923	4.429119190992483
8	86.2265796392691	3.352550371836089	3.894203918306206	6.5266660705886
9	79.56450018206297	7.44255297643807	7.897904785599791	5.095042055899164
10	30.981805364961758	38.44457316275134	13.818255797831053	16.755365674455852
11	23.452302740274533	26.823253782828026	30.314586678173566	19.409856798723872
12	25.254143066510647	23.470185082239336	32.50063819227664	18.77503365897337
13	25.697702896550652	25.577709790323063	28.066724460322583	20.657862852803703
14	21.335707279020255	26.968904162309464	30.399722175788607	21.295666382881677
15	19.794197568778987	29.290628227406373	28.097694603290613	22.81747960052403
16	22.09946469598075	27.66294636204486	27.297654173647906	22.939934768326484
17	20.83383546431242	27.17973438243073	28.918079436472983	23.068350716783872
18	21.718363480629407	26.05042561269698	28.570928754417135	23.660282152256478
19	21.527100170918906	29.039757111146532	28.275870111997886	21.15727260593668
20	22.513091040557928	29.067617281598938	27.616685520875045	20.802606156968086
21	23.50024814989031	27.46313062791641	27.03602773577155	22.000593486421728
22	22.361091133857105	26.287690599200996	26.998578483395985	24.35263978354591
23	22.0995942781689	27.553708577433778	25.97410170387619	24.372595440521128
24	19.602675094692184	30.20612638669139	27.65983638952924	22.531362129087185
25	22.125121969234595	27.328753898804088	26.128563672151884	24.417560459809437
26	23.461373493445084	27.49280494900293	25.13946282999724	23.906358727554743
27	20.107008970974885	27.062203337778	27.77309122197299	25.05769646927412
28	23.694103103363823	25.635633028426447	27.603727302059966	23.066536566149765
29	21.048812314454505	27.559798940276863	26.05081435926143	25.340574386007198
30	24.22305759539517	26.53856171546084	26.383192671868095	22.8551880172759
31	25.12741168649922	27.752228489680725	23.996677512695815	23.12368231112424
32	22.331287230582433	25.86266102206655	25.87847004902094	25.927581698330076
33	24.478204923863984	26.035134914495195	26.48102722392191	23.00563293771891
34	22.184211447031338	26.090077762271108	26.119363336793178	25.606347453904377
35	22.450373261492967	25.672304787673106	29.116858513096222	22.7604634377377
36	22.77057084841346	28.23829127743417	26.868866713057088	22.12227116109528
37	21.47772935723347	28.892551745407285	25.59844294042718	24.031275956932063
38	22.703706439327675	26.783990379818352	28.611747143684617	21.900556037169356
39	20.76982186336595	26.165365013586694	27.63715950660286	25.427653616444495
40	22.559092717351444	26.757685195623747	28.60358346583112	22.079638621193684
41	21.33609602558471	25.602071241695402	30.119565485006696	22.9422672477132
42	22.819941662098895	26.689136218092006	26.96112923102042	23.529792888788677
43	21.285429390017764	24.87576307711047	28.957083675106354	24.881723857765405
44	22.408906961284732	24.5328886072636	27.55928061152426	25.49892381992741
45	24.825614770296134	24.90336408318658	27.81585334406274	22.455167802454547
46	20.870377641370926	24.54869763421799	30.63050805288508	23.950416671526
47	21.471898158766688	27.384862986273355	27.305947433689553	23.837291421270397
48	21.11217800446022	25.206586403459326	30.207163044196594	23.47407254788386
49	22.035710259410582	25.025689668800887	28.624187033747088	24.314413038041444
50	20.653975387159182	24.52938988818353	28.569503350347475	26.247131374309813
51	21.121637504195224	22.53239878659239	30.943060290704683	25.402903418507705
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1133.0
1	922.5
2	712.0
3	481.5
4	251.0
5	267.5
6	284.0
7	302.5
8	321.0
9	386.5
10	452.0
11	523.5
12	595.0
13	622.5
14	650.0
15	691.5
16	733.0
17	879.5
18	1026.0
19	1139.0
20	1252.0
21	1576.5
22	1901.0
23	2089.5
24	2278.0
25	3046.0
26	5722.0
27	7630.0
28	8622.0
29	9614.0
30	11479.5
31	13345.0
32	16012.0
33	18679.0
34	21785.0
35	24891.0
36	26405.5
37	27920.0
38	30711.5
39	33503.0
40	34646.5
41	35790.0
42	37838.0
43	39886.0
44	51546.0
45	63206.0
46	66464.5
47	69723.0
48	72210.0
49	74697.0
50	71973.0
51	69249.0
52	63607.5
53	57966.0
54	51794.0
55	45622.0
56	41315.5
57	37009.0
58	33818.0
59	30627.0
60	27590.0
61	24553.0
62	22968.0
63	21383.0
64	19601.0
65	17819.0
66	14589.0
67	11359.0
68	9552.5
69	7746.0
70	6376.5
71	5007.0
72	4171.0
73	3335.0
74	2760.5
75	1906.5
76	1627.0
77	1253.0
78	879.0
79	758.5
80	638.0
81	456.5
82	275.0
83	180.0
84	85.0
85	57.5
86	30.0
87	25.0
88	20.0
89	12.0
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	771711.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.581015247366857
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.24915864171149	18.993688593177822
2	10.158721745120486	5.197408317113688
3	3.607051200494084	2.768160952736163
4	1.7553487523730933	1.7961441279560995
5	1.0388446627877035	1.328735057920896
6	0.6648399627190038	1.020436873402419
7	0.5529473878103006	0.9901468890996881
8	0.4578284100525169	0.936937243058493
9	0.38646160706144045	0.8897472236484546
>10	5.694611002304977	36.018691341587164
>50	1.321711381045499	22.351528139533823
>100	0.1053501177806319	4.010938123522116
>500	0.004071502136449542	0.7810184952937281
>1k	0.0015268133011685783	0.806666210508408
>5k	0.0015268133011685783	2.1097524114410513
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC	5838	0.7565008144240526	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	5232	0.6779740084047008	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGT	5135	0.6654045361540785	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	3897	0.5049817872234554	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	1283	0.16625394739740654	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	1016	0.13165550316115748	No Hit
AACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	983	0.12737929095218287	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	890	0.11532814745416355	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATTC	819	0.1061278120954606	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT	773	0.10016703144052631	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5888214629569878	0.0
2	0.0	0.0	0.0	0.8206439975586716	0.0
3	0.006090362843085041	0.0	0.0	1.1756991930917144	0.0
4	0.006090362843085041	0.0	0.0	2.8298158248359813	0.0
5	0.006090362843085041	0.0	0.0	3.042330613403204	0.0
6	0.006090362843085041	0.0	0.0	4.208959053324366	0.0
7	0.006090362843085041	0.0	0.0	5.048781214729348	0.0
8	0.006090362843085041	0.0	0.0	5.787918015941201	0.0
9	0.006090362843085041	0.0	0.0	7.361952855408307	0.0
10	0.006090362843085041	0.0	0.0	8.743817309847858	0.0
11	0.006090362843085041	0.0	0.0	10.661115365726289	0.0
12	0.006090362843085041	0.0	0.0	11.25926674623013	0.0
13	0.006090362843085041	0.0	0.0	11.547457532677388	0.0
14	0.006090362843085041	0.0	0.0	11.929724987722087	0.0
15	0.006090362843085041	0.0	0.0	12.199385521263789	0.0
16	0.006090362843085041	0.0	0.0	12.710198506954027	0.0
17	0.006090362843085041	0.0	0.0	13.335821311345828	0.0
18	0.006090362843085041	0.0	0.0	14.249375737808585	0.0
19	0.006090362843085041	0.0	0.0	14.613501686512178	0.0
20	0.006090362843085041	0.0	0.0	15.005099059103731	0.0
21	0.006090362843085041	0.0	0.0	15.43064696499078	0.0
22	0.006090362843085041	0.0	0.0	15.901030307977987	0.0
23	0.006090362843085041	0.0	0.0	16.366878274379918	0.0
24	0.006090362843085041	0.0	0.0	16.710789401731997	0.0
25	0.006090362843085041	0.0	0.0	17.006107208527546	0.0
26	0.006090362843085041	0.0	0.0	17.30932952880029	0.0
27	0.006090362843085041	0.0	0.0	17.63626538950462	0.0
28	0.006090362843085041	0.0	0.0	17.958277127059223	0.0
29	0.006090362843085041	0.0	0.0	18.301799507846848	0.0
30	0.006090362843085041	0.0	0.0	18.78306775463872	0.0
31	0.006349527219386532	0.0	0.0	19.097174978716126	0.0
32	0.006349527219386532	0.0	0.0	19.414262593121	0.0
33	0.006349527219386532	0.0	0.0	19.748973385114375	0.0
34	0.006349527219386532	0.0	0.0	20.082777101790697	0.0
35	0.006349527219386532	0.0	0.0	20.463359988389435	0.0
36	0.006349527219386532	0.0	0.0	20.774098075574923	0.0
37	0.006349527219386532	0.0	0.0	21.095721066565073	0.0
38	0.006349527219386532	0.0	0.0	21.425766899785025	0.0
39	0.006349527219386532	0.0	0.0	21.77304716402902	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTCG	30	2.16457E-6	45.000004	1
TGATTCG	30	2.16457E-6	45.000004	15
TCATACG	30	2.16457E-6	45.000004	1
GTAACGA	30	2.16457E-6	45.000004	29
ATTTACG	25	3.8896098E-5	45.0	1
AACGTAG	40	6.8102963E-9	45.0	1
TAATACG	40	6.8102963E-9	45.0	1
AGGTACG	25	3.8896098E-5	45.0	1
TCGTTAC	20	7.031845E-4	45.0	33
CGTATTG	20	7.031845E-4	45.0	1
ACGTTAG	20	7.031845E-4	45.0	1
TTAGCGA	20	7.031845E-4	45.0	27
TAGCGAT	20	7.031845E-4	45.0	28
ACGATAG	35	1.2112287E-7	45.0	1
CGTAGTC	20	7.031845E-4	45.0	16
TACGATA	25	3.8896098E-5	45.0	35
CTACGCG	20	7.031845E-4	45.0	1
ACTACGC	20	7.031845E-4	45.0	18
TCCACGA	20	7.031845E-4	45.0	36
CGCAGTC	25	3.8896098E-5	45.0	16
>>END_MODULE