FastQCFastQC Report
Sat 18 Jun 2016
SRR3548489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780292
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC57960.7427988496614089No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT51830.6642385158376608No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGT50820.651294643543699No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT41320.5295453496896034No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT13240.16968006848718173No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT10470.13418053754235593No Hit
AACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG10000.12815715142536382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.10496070701737298No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC207.031892E-445.00000437
AGGCGTA207.031892E-445.00000438
TAGCCCG302.164601E-645.0000041
ATCCGCG302.164601E-645.0000041
TACGCCG207.031892E-445.0000041
TCTCGAC207.031892E-445.00000445
CCATACG351.211265E-745.0000041
AACGATC302.164601E-645.00000413
CATACCG207.031892E-445.0000041
TATAGCG207.031892E-445.0000041
TCGAGAC207.031892E-445.00000429
CACGTTG207.031892E-445.00000440
CGACTAG406.8102963E-945.0000041
ATACCCG453.8380676E-1045.0000041
ACGTTAG253.8896495E-545.01
ATTACCG253.8896495E-545.01
TCGGTAG253.8896495E-545.015
TAGCACG850.042.3529431
CCTAACG603.6379788E-1241.2500041
TACGAGT556.002665E-1140.90909215