##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548489_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	780292
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.942251105996217	33.0	31.0	34.0	30.0	34.0
2	32.13403700153276	34.0	31.0	34.0	30.0	34.0
3	32.26289260943339	34.0	31.0	34.0	30.0	34.0
4	35.83069415039498	37.0	35.0	37.0	35.0	37.0
5	35.6602861492877	37.0	35.0	37.0	33.0	37.0
6	35.659883735832224	37.0	35.0	37.0	33.0	37.0
7	35.71038790606593	37.0	35.0	37.0	35.0	37.0
8	35.91142162164933	37.0	35.0	37.0	35.0	37.0
9	37.543179989029746	39.0	37.0	39.0	35.0	39.0
10	37.09100951951321	39.0	37.0	39.0	33.0	39.0
11	37.080216124220165	39.0	37.0	39.0	33.0	39.0
12	37.009114536609374	39.0	37.0	39.0	33.0	39.0
13	37.00839301184685	39.0	37.0	39.0	33.0	39.0
14	38.200797906424775	40.0	37.0	41.0	33.0	41.0
15	38.217901759854	40.0	37.0	41.0	33.0	41.0
16	38.12398435457495	40.0	37.0	41.0	33.0	41.0
17	38.11360747002404	40.0	37.0	41.0	33.0	41.0
18	38.03509968063238	40.0	37.0	41.0	33.0	41.0
19	38.02819201017055	40.0	37.0	41.0	33.0	41.0
20	38.0164182126691	40.0	37.0	41.0	33.0	41.0
21	38.018730167680815	40.0	37.0	41.0	33.0	41.0
22	37.99836855946236	40.0	37.0	41.0	33.0	41.0
23	37.89203272621019	40.0	37.0	41.0	33.0	41.0
24	37.79139860462494	40.0	37.0	41.0	33.0	41.0
25	37.80605209331891	40.0	37.0	41.0	33.0	41.0
26	37.736051375638866	40.0	37.0	41.0	33.0	41.0
27	37.65956077981063	40.0	37.0	41.0	33.0	41.0
28	37.63572483121703	40.0	37.0	41.0	33.0	41.0
29	37.50883259087624	40.0	36.0	41.0	32.0	41.0
30	37.49623986917718	40.0	36.0	41.0	32.0	41.0
31	37.42334023673189	40.0	36.0	41.0	32.0	41.0
32	37.38742035033039	40.0	36.0	41.0	32.0	41.0
33	37.35520676874811	40.0	36.0	41.0	32.0	41.0
34	37.239116894700956	39.0	36.0	41.0	31.0	41.0
35	37.19667124614888	39.0	36.0	41.0	31.0	41.0
36	37.15910582192308	39.0	36.0	41.0	31.0	41.0
37	37.03237634116459	39.0	35.0	41.0	31.0	41.0
38	37.017433217308394	39.0	35.0	41.0	31.0	41.0
39	36.957588953878805	39.0	35.0	41.0	31.0	41.0
40	36.85155300836097	39.0	35.0	41.0	31.0	41.0
41	36.82592285964741	39.0	35.0	41.0	31.0	41.0
42	36.75059464918262	39.0	35.0	41.0	31.0	41.0
43	36.70649449180563	39.0	35.0	40.0	31.0	41.0
44	36.61012159550527	39.0	35.0	40.0	31.0	41.0
45	36.425529160878234	39.0	35.0	40.0	30.0	41.0
46	36.25405232912807	38.0	35.0	40.0	30.0	41.0
47	36.1564734740328	38.0	35.0	40.0	29.0	41.0
48	36.14543786172356	38.0	35.0	40.0	30.0	41.0
49	35.99745992525875	38.0	35.0	40.0	29.0	41.0
50	35.83865399107001	38.0	34.0	40.0	29.0	41.0
51	34.66574051765237	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	6.0
11	3.0
12	6.0
13	10.0
14	9.0
15	24.0
16	51.0
17	98.0
18	199.0
19	396.0
20	695.0
21	1129.0
22	1642.0
23	2047.0
24	2845.0
25	3599.0
26	4473.0
27	5962.0
28	7515.0
29	9910.0
30	13074.0
31	17146.0
32	23022.0
33	30259.0
34	44815.0
35	56399.0
36	69045.0
37	99424.0
38	161943.0
39	224459.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.480794369287395	33.55679668636869	19.328661577973374	26.633747366370535
2	23.549389203016307	31.215365529827295	19.80976352442419	25.42548174273221
3	21.448637176851744	29.43769768240607	22.538998221178737	26.574666919563445
4	22.22385978582377	30.53626078442429	18.61751754471403	28.62236188503791
5	18.55843709790694	34.821066985179904	17.31749140065514	29.303004516258017
6	21.03904692089628	35.18900616692213	21.452610048545928	22.319336863635662
7	84.92935977813434	5.411563876087413	5.268540495096707	4.39053585068154
8	86.39112024729204	3.385783783506687	3.8553515863292205	6.367744382872054
9	79.6333936526326	7.450800469567803	7.889610556048249	5.026195321751344
10	31.087336535553355	38.39178153819339	13.85827356938172	16.66260835687153
11	23.442506138727552	26.946578972999852	30.290327210839017	19.320587677433576
12	25.136615523419437	23.516324657948562	32.491938915175346	18.855120903456655
13	25.678079488191603	25.558124394457458	28.1604835113009	20.603312606050043
14	21.417879460509656	27.04461919384025	30.362607844242923	21.174893501407166
15	19.803740138307198	29.23328702588262	28.21469398635383	22.74827884945636
16	22.01983360075459	27.639140219302515	27.40204948916559	22.9389766907773
17	20.876415495737493	27.207122461847614	28.887262717034133	23.029199325380755
18	21.713025380242268	26.04358368405674	28.66055271616267	23.582838219538328
19	21.586662428936858	28.954545221532452	28.170736083414926	21.288056266115763
20	22.588082410174653	29.137553633767872	27.636705233425435	20.63765872263204
21	23.43789248127624	27.410251546856816	27.042953150871725	22.108902820995215
22	22.34163620798368	26.218646352903786	27.057434908982792	24.382282530129746
23	22.151963623874142	27.50175575297453	26.098434944866796	24.24784567828454
24	19.595997395846684	30.207794005321087	27.68220102218144	22.514007576650794
25	22.049950531339547	27.328359127096007	26.172766092693507	24.448924248870934
26	23.406365822025602	27.483044808866424	25.18851916974671	23.922070199361265
27	20.125799059839135	27.02731797839783	27.844960604491654	25.00192235727138
28	23.551439717439113	25.7220373911305	27.663874549527613	23.062648341902776
29	21.08274850953233	27.525721140291072	26.14136759059429	25.250162759582313
30	24.27988496614088	26.468809112486095	26.49687552864825	22.754430392724775
31	25.14481758111066	27.59723283078642	24.15159965756409	23.106349930538826
32	22.285503375659367	25.92657620480538	25.999113152512138	25.788807267023113
33	24.290522009709186	26.078826900698715	26.474319869997387	23.156331219594716
34	22.148503380785655	26.114070117340688	26.171100049724977	25.566326452148687
35	22.521568848584888	25.60233861169921	29.104232774397275	22.771859765318624
36	22.702142274943228	28.30530109241156	26.83854249434827	22.154014138296947
37	21.491954294033516	28.833693027738335	25.696790432299704	23.977562245928446
38	22.704577260820308	26.89057429782697	28.587118668395934	21.81772977295679
39	20.87603102428322	26.08959210141844	27.625299246948575	25.409077627349763
40	22.539639006935865	26.82008786454302	28.551106508845407	22.089166619675712
41	21.330219968934706	25.667698758926143	30.138460986399963	22.863620285739184
42	22.82619839752298	26.80035166322351	26.793943805652244	23.579506133601267
43	21.29305439502135	24.996539756911513	29.01157515391674	24.698830694150395
44	22.543868192932905	24.4891655944185	27.425245933573585	25.541720279075015
45	24.749965397569117	24.861333962157754	27.963121498105835	22.42557914216729
46	20.953566100895564	24.649362033700204	30.47410456598299	23.92296729942124
47	21.551419212294885	27.36616548676649	27.240571478369635	23.841843822568986
48	21.158617543176145	25.303732448878115	30.068102710267436	23.469547297678307
49	22.043158202314007	25.105217021320225	28.595833354692857	24.25579142167291
50	20.712502499064453	24.57541535732777	28.451784716490753	26.26029742711703
51	21.10594495394032	22.64152394231903	30.96968827054487	25.28284283319578
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1055.0
1	882.0
2	709.0
3	497.0
4	285.0
5	286.5
6	288.0
7	290.0
8	292.0
9	364.0
10	436.0
11	527.5
12	619.0
13	634.5
14	650.0
15	714.0
16	778.0
17	897.0
18	1016.0
19	1115.5
20	1215.0
21	1621.0
22	2027.0
23	2166.0
24	2305.0
25	3020.0
26	5684.0
27	7633.0
28	8789.5
29	9946.0
30	11684.0
31	13422.0
32	16541.0
33	19660.0
34	22183.0
35	24706.0
36	26378.5
37	28051.0
38	31172.5
39	34294.0
40	35302.0
41	36310.0
42	38301.0
43	40292.0
44	51880.5
45	63469.0
46	66939.0
47	70409.0
48	72944.5
49	75480.0
50	72839.5
51	70199.0
52	64458.0
53	58717.0
54	52544.5
55	46372.0
56	42043.0
57	37714.0
58	34114.0
59	30514.0
60	27650.0
61	24786.0
62	23224.0
63	21662.0
64	19780.5
65	17899.0
66	14717.5
67	11536.0
68	9619.0
69	7702.0
70	6399.0
71	5096.0
72	4185.5
73	3275.0
74	2708.5
75	1916.5
76	1691.0
77	1272.5
78	854.0
79	754.5
80	655.0
81	449.0
82	243.0
83	172.0
84	101.0
85	62.0
86	23.0
87	17.5
88	12.0
89	8.5
90	5.0
91	5.5
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	780292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.43633740357859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.43906307617836	18.934571244119418
2	10.17149929510407	5.174513759410578
3	3.469153892940007	2.6472770677728055
4	1.7178099958093347	1.7477917859458465
5	0.9629907960400953	1.22474794023083
6	0.7458755902014331	1.1383405904074182
7	0.525316628520604	0.935349170473231
8	0.4385031438852766	0.8923131136316691
9	0.35981596236266467	0.8237160199665068
>10	5.723158383489448	36.10308421070876
>50	1.3302573531017055	22.610508749277397
>100	0.10946117648374869	4.120235688575492
>500	0.004054117647546248	0.7389973470968367
>1k	0.001520294117829843	0.8382521800402458
>5k	0.001520294117829843	2.0703011323429785
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCC	5796	0.7427988496614089	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT	5183	0.6642385158376608	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGT	5082	0.651294643543699	No Hit
CTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGCT	4132	0.5295453496896034	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT	1324	0.16968006848718173	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT	1047	0.13418053754235593	No Hit
AACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTG	1000	0.12815715142536382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	819	0.10496070701737298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6004162544278295	0.0
2	0.0	0.0	0.0	0.8199494548194778	0.0
3	0.00499812890558919	0.0	0.0	1.166614549425087	0.0
4	0.00499812890558919	0.0	0.0	2.8068979305183186	0.0
5	0.00499812890558919	0.0	0.0	3.026046659455691	0.0
6	0.00499812890558919	0.0	0.0	4.1743347362269505	0.0
7	0.00499812890558919	0.0	0.0	5.003255191646204	0.0
8	0.00499812890558919	0.0	0.0	5.742850112521979	0.0
9	0.00499812890558919	0.0	0.0	7.288297201560441	0.0
10	0.00499812890558919	0.0	0.0	8.656631107329051	0.0
11	0.00499812890558919	0.0	0.0	10.547846190913145	0.0
12	0.00499812890558919	0.0	0.0	11.159796588969257	0.0
13	0.00499812890558919	0.0	0.0	11.446612293859221	0.0
14	0.00499812890558919	0.0	0.0	11.811475703967233	0.0
15	0.00499812890558919	0.0	0.0	12.068815264029363	0.0
16	0.00499812890558919	0.0	0.0	12.584519641365027	0.0
17	0.00499812890558919	0.0	0.0	13.213643097712138	0.0
18	0.00499812890558919	0.0	0.0	14.090494327764478	0.0
19	0.00499812890558919	0.0	0.0	14.463431638412287	0.0
20	0.00499812890558919	0.0	0.0	14.844058378145617	0.0
21	0.00499812890558919	0.0	0.0	15.271462478149205	0.0
22	0.00499812890558919	0.0	0.0	15.751667324540044	0.0
23	0.00499812890558919	0.0	0.0	16.20739415500864	0.0
24	0.00499812890558919	0.0	0.0	16.56315840736545	0.0
25	0.00499812890558919	0.0	0.0	16.86138009873227	0.0
26	0.00499812890558919	0.0	0.0	17.16152414737047	0.0
27	0.00499812890558919	0.0	0.0	17.484223854659536	0.0
28	0.00499812890558919	0.0	0.0	17.803847790314396	0.0
29	0.00499812890558919	0.0	0.0	18.146540013225817	0.0
30	0.00499812890558919	0.0	0.0	18.61969621628826	0.0
31	0.00499812890558919	0.0	0.0	18.93380939443183	0.0
32	0.00499812890558919	0.0	0.0	19.257149887478022	0.0
33	0.00499812890558919	0.0	0.0	19.59330609566675	0.0
34	0.00499812890558919	0.0	0.0	19.919594203195725	0.0
35	0.00499812890558919	0.0	0.0	20.297017014143425	0.0
36	0.00499812890558919	0.0	0.0	20.600749463021536	0.0
37	0.00499812890558919	0.0	0.0	20.918322884253588	0.0
38	0.00499812890558919	0.0	0.0	21.25242857801951	0.0
39	0.00499812890558919	0.0	0.0	21.590763457782472	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTCGC	20	7.031892E-4	45.000004	37
AGGCGTA	20	7.031892E-4	45.000004	38
TAGCCCG	30	2.164601E-6	45.000004	1
ATCCGCG	30	2.164601E-6	45.000004	1
TACGCCG	20	7.031892E-4	45.000004	1
TCTCGAC	20	7.031892E-4	45.000004	45
CCATACG	35	1.211265E-7	45.000004	1
AACGATC	30	2.164601E-6	45.000004	13
CATACCG	20	7.031892E-4	45.000004	1
TATAGCG	20	7.031892E-4	45.000004	1
TCGAGAC	20	7.031892E-4	45.000004	29
CACGTTG	20	7.031892E-4	45.000004	40
CGACTAG	40	6.8102963E-9	45.000004	1
ATACCCG	45	3.8380676E-10	45.000004	1
ACGTTAG	25	3.8896495E-5	45.0	1
ATTACCG	25	3.8896495E-5	45.0	1
TCGGTAG	25	3.8896495E-5	45.0	15
TAGCACG	85	0.0	42.352943	1
CCTAACG	60	3.6379788E-12	41.250004	1
TACGAGT	55	6.002665E-11	40.909092	15
>>END_MODULE