FastQCFastQC Report
Sat 18 Jun 2016
SRR3548487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887888
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT44400.5000630710179662Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC35370.39836105454742043No Hit
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT33770.3803407636999261No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT33020.3718937523651632No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC9930.11183843007226138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9290.10463031373326365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG351.2115015E-745.0000041
CGAAACG351.2115015E-745.0000041
ACGCGAG502.1827873E-1145.01
TAATACG207.032419E-445.01
ACTATCG207.032419E-445.01
CCGATCA207.032419E-445.010
TTAGCGT207.032419E-445.043
TAGAACG406.8121153E-945.01
TAGCGCG551.8189894E-1245.01
CGGATAA253.8900856E-545.028
ACGTCTA207.032419E-445.019
CGTAACG453.8380676E-1045.01
CGGTCTA253.8900856E-545.031
ATTGCGA207.032419E-445.029
TATACCG253.8900856E-545.01
CGATCGA207.032419E-445.040
CAATACG502.1827873E-1145.01
TCAAACG253.8900856E-545.01
CTAGACG302.1649412E-644.9999961
CTATCGG302.1649412E-644.9999962