##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	887888
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.974367262537616	33.0	31.0	34.0	30.0	34.0
2	32.16042901807435	34.0	31.0	34.0	30.0	34.0
3	32.30715135242283	34.0	31.0	34.0	30.0	34.0
4	35.86876272683041	37.0	35.0	37.0	35.0	37.0
5	35.70951966914746	37.0	35.0	37.0	33.0	37.0
6	35.71185554934856	37.0	35.0	37.0	33.0	37.0
7	35.7968673976898	37.0	35.0	37.0	35.0	37.0
8	35.97388071468473	37.0	35.0	37.0	35.0	37.0
9	37.589538320148485	39.0	37.0	39.0	35.0	39.0
10	37.14277701692105	39.0	37.0	39.0	34.0	39.0
11	37.16108337988575	39.0	37.0	39.0	34.0	39.0
12	37.12742936045988	39.0	37.0	39.0	33.0	39.0
13	37.09908794802948	39.0	37.0	39.0	33.0	39.0
14	38.29017398590813	40.0	38.0	41.0	33.0	41.0
15	38.29349985583767	40.0	38.0	41.0	33.0	41.0
16	38.22055934982791	40.0	38.0	41.0	33.0	41.0
17	38.19896428378354	40.0	37.0	41.0	33.0	41.0
18	38.16047970014236	40.0	37.0	41.0	33.0	41.0
19	38.14443037860631	40.0	37.0	41.0	33.0	41.0
20	38.11466761573532	40.0	37.0	41.0	33.0	41.0
21	38.11574995945435	40.0	37.0	41.0	33.0	41.0
22	38.089051772295605	40.0	37.0	41.0	33.0	41.0
23	38.001310976159154	40.0	37.0	41.0	33.0	41.0
24	37.92502320112447	40.0	37.0	41.0	33.0	41.0
25	37.945327563836884	40.0	37.0	41.0	33.0	41.0
26	37.89195371488296	40.0	37.0	41.0	33.0	41.0
27	37.82628890130287	40.0	37.0	41.0	33.0	41.0
28	37.78531188618384	40.0	37.0	41.0	33.0	41.0
29	37.65753000378426	40.0	37.0	41.0	33.0	41.0
30	37.66122866848071	40.0	37.0	41.0	33.0	41.0
31	37.615909889535615	40.0	37.0	41.0	33.0	41.0
32	37.58040653776152	40.0	37.0	41.0	33.0	41.0
33	37.53650460418431	40.0	37.0	41.0	33.0	41.0
34	37.48303164363073	40.0	36.0	41.0	32.0	41.0
35	37.39756253040924	40.0	36.0	41.0	32.0	41.0
36	37.37046902312003	40.0	36.0	41.0	32.0	41.0
37	37.245907141441265	39.0	36.0	41.0	31.0	41.0
38	37.20417665291118	39.0	36.0	41.0	32.0	41.0
39	37.14327482745571	39.0	36.0	41.0	31.0	41.0
40	37.04569269998018	39.0	35.0	41.0	31.0	41.0
41	36.99602990467266	39.0	35.0	41.0	31.0	41.0
42	36.88807822608257	39.0	35.0	41.0	31.0	41.0
43	36.82592962175409	39.0	35.0	41.0	31.0	41.0
44	36.784387220009734	39.0	35.0	41.0	31.0	41.0
45	36.64334578235093	39.0	35.0	40.0	31.0	41.0
46	36.47841394410106	39.0	35.0	40.0	30.0	41.0
47	36.36584681851765	39.0	35.0	40.0	30.0	41.0
48	36.32560075144613	39.0	35.0	40.0	30.0	41.0
49	36.140347656461174	38.0	35.0	40.0	29.0	41.0
50	35.9789230173175	38.0	35.0	40.0	29.0	41.0
51	34.73155172724488	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	3.0
11	6.0
12	6.0
13	7.0
14	12.0
15	22.0
16	49.0
17	108.0
18	188.0
19	369.0
20	639.0
21	1081.0
22	1590.0
23	2183.0
24	2875.0
25	3705.0
26	4780.0
27	6145.0
28	8129.0
29	10415.0
30	14036.0
31	18321.0
32	24383.0
33	32492.0
34	49215.0
35	62362.0
36	75777.0
37	111710.0
38	189263.0
39	267943.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.24355774602202	32.008654244679505	20.148149316129963	26.599638693168508
2	22.92158470437713	30.653415746130143	20.55687203791469	25.868127511578038
3	20.98485394554268	29.589993332492387	22.249315228947793	27.175837493017134
4	21.496067071522535	30.207526174472456	19.935735137765125	28.360671616239884
5	18.995864343250503	33.58362766475051	17.497477159281352	29.92303083271764
6	20.843507289207647	35.614852323716505	20.093412682680697	23.44822770439515
7	85.7216225469879	4.792496350891104	4.978555853891482	4.507325248229507
8	86.89035103526571	3.541325031986016	4.01829960535563	5.550024327392644
9	79.26258717315697	7.8764438758041555	8.162628619825924	4.698340331212946
10	30.28568918602346	40.914619861964574	13.195357973077684	15.604332978934279
11	22.669188005694412	26.90249220622421	31.51647505090732	18.911844737174057
12	25.983457373002	22.97001423602977	32.35791000666751	18.688618384300725
13	26.338907609968825	23.455097940280755	28.78887877750347	21.41711567224695
14	20.63109311084281	26.646266195736402	31.148748490800642	21.57389220262015
15	20.16673274106644	29.005460148126787	29.83428090029373	20.99352621051304
16	21.969662840358243	26.818022092876582	29.838335465734417	21.37397960103076
17	21.191636783017678	26.83581713008848	29.758032544645268	22.21451354224857
18	21.955471861315843	26.28169318652803	28.20085416178617	23.561980790369958
19	22.16405672787559	29.114145027300744	28.69235759465158	20.029440650172095
20	23.54407312634026	27.502567891445768	28.03765790279855	20.91570107941542
21	23.635075595120107	27.110626565512767	27.827608887607447	21.426688951759683
22	21.1195556196277	26.500639720325086	28.52521939704107	23.854585263006143
23	22.026989890616836	27.49265673147965	26.450295532769896	24.03005784513362
24	20.329816373236266	28.24297659164219	27.967378768493322	23.459828266628225
25	21.216302056115186	27.916696700484746	26.71474330095688	24.152257942443192
26	22.609383165444292	28.16177265601067	26.556389995134523	22.67245418341052
27	20.177995422846124	27.007347773593064	28.612730434469213	24.2019263690916
28	21.825275259942696	26.583983565494744	29.924382354531204	21.666358820031355
29	23.291676427657542	27.263686410898675	26.778940587101076	22.665696574342707
30	22.890274449029608	25.079965040635756	29.03789667165228	22.991863838682356
31	23.432459949903592	25.98176797073505	27.359982340114968	23.22578973924639
32	22.718518551889428	25.697272628980233	27.41134016903033	24.172868650100014
33	23.117330113708036	25.7767871623448	29.55057394626349	21.55530877768367
34	22.116077703494135	25.304430288504857	29.539649144937197	23.039842863063807
35	23.05268232029265	25.767101256014275	29.28364838808498	21.896568035608095
36	23.277034941343953	25.97568612257402	30.032278845980574	20.715000090101455
37	22.7765213630548	27.507072964157643	27.404807813598108	22.311597859189447
38	22.777084497143786	25.888062458328076	29.135769376317732	22.199083668210402
39	23.58642080983187	24.617406699944137	27.39872596543708	24.397446524786908
40	24.617857207215323	26.063872920908942	27.529035193628026	21.789234678247706
41	22.40552862523201	24.870479159533634	30.87303804083398	21.850954174400375
42	23.587884958463228	26.785360315715494	27.518335645937324	22.108419079883948
43	22.106053916710216	25.270980123619196	29.079793847872704	23.543172111797883
44	22.371064818986177	24.55073162380841	28.901956102571496	24.176247454633916
45	23.5354008613699	24.509172328041377	29.404722217216584	22.550704593372135
46	21.146811309534534	25.109022759627344	30.530089380642604	23.21407655019552
47	22.24312075396897	25.292153965365	28.881795902185864	23.58292937848017
48	22.670764781143568	24.07600958679473	31.09254770872002	22.16067792334168
49	21.627164687438054	24.504779882147297	29.304596976195196	24.56345845421945
50	21.38963472870452	23.67336871317103	28.788315643414485	26.148680914709963
51	21.312372731695888	23.14278377453012	31.180058746148166	24.364784747625826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1306.0
1	1099.5
2	893.0
3	688.0
4	483.0
5	476.5
6	470.0
7	517.5
8	565.0
9	660.5
10	756.0
11	763.0
12	770.0
13	894.0
14	1018.0
15	1037.5
16	1057.0
17	1248.5
18	1440.0
19	1536.5
20	1633.0
21	1894.5
22	2156.0
23	3144.5
24	4133.0
25	4488.5
26	6908.5
27	8973.0
28	10334.5
29	11696.0
30	13775.0
31	15854.0
32	19657.0
33	23460.0
34	24824.0
35	26188.0
36	29352.0
37	32516.0
38	34896.0
39	37276.0
40	41207.0
41	45138.0
42	48445.5
43	51753.0
44	59774.0
45	67795.0
46	74353.5
47	80912.0
48	85710.5
49	90509.0
50	85248.5
51	79988.0
52	71353.0
53	62718.0
54	57031.0
55	51344.0
56	45909.0
57	40474.0
58	38301.5
59	36129.0
60	33196.0
61	30263.0
62	27496.0
63	24729.0
64	21129.5
65	17530.0
66	14220.5
67	10911.0
68	9051.0
69	7191.0
70	6064.0
71	4937.0
72	4030.0
73	3123.0
74	2577.5
75	1675.0
76	1318.0
77	1007.5
78	697.0
79	528.0
80	359.0
81	302.5
82	246.0
83	187.0
84	128.0
85	126.0
86	124.0
87	72.5
88	21.0
89	19.5
90	18.0
91	12.0
92	6.0
93	5.5
94	5.0
95	2.5
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	887888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.602424290570237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71652921275923	20.34754916558796
2	10.297716415864182	5.684838754693067
3	3.6518052406945563	3.0239603304053735
4	1.8922257940541705	2.0892007688417755
5	1.1304929661590988	1.560217325471435
6	0.8079189128044371	1.3380312374162575
7	0.5811182172616666	1.1228190117085408
8	0.4797426914283913	1.0593649055285257
9	0.4289915476410657	1.0657086043551363
>10	5.927088784742892	39.195170765115904
>50	1.0120849847247875	18.056556533504743
>100	0.06894982847187907	3.0770381487283442
>500	0.003693740810993521	0.7192443563167755
>1k	0.0016416625826637873	1.6603000923261402
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT	4440	0.5000630710179662	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC	3537	0.39836105454742043	No Hit
AATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT	3377	0.3803407636999261	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGT	3302	0.3718937523651632	No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC	993	0.11183843007226138	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	929	0.10463031373326365	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2525363559367848E-4	0.0	0.0	0.5713558466833655	0.0
2	2.2525363559367848E-4	0.0	0.0	0.8346773466923756	0.0
3	2.2525363559367848E-4	0.0	0.0	1.15521327014218	0.0
4	2.2525363559367848E-4	0.0	0.0	2.5892905411493343	0.0
5	2.2525363559367848E-4	0.0	0.0	2.812291640387076	0.0
6	2.2525363559367848E-4	0.0	0.0	4.070333195177771	0.0
7	2.2525363559367848E-4	0.0	0.0	4.991057430666931	0.0
8	2.2525363559367848E-4	0.0	0.0	5.842854053664426	0.0
9	2.2525363559367848E-4	0.0	0.0	7.140878128772998	0.0
10	2.2525363559367848E-4	0.0	0.0	8.68262663759393	0.0
11	2.2525363559367848E-4	0.0	0.0	10.276746616690394	0.0
12	2.2525363559367848E-4	0.0	0.0	10.951156001657866	0.0
13	2.2525363559367848E-4	0.0	0.0	11.265384823311049	0.0
14	2.2525363559367848E-4	0.0	0.0	11.647527616095724	0.0
15	2.2525363559367848E-4	0.0	0.0	11.93202295785054	0.0
16	2.2525363559367848E-4	0.0	0.0	12.482317589605897	0.0
17	2.2525363559367848E-4	0.0	0.0	13.154136557764042	0.0
18	2.2525363559367848E-4	0.0	0.0	14.019110518443767	0.0
19	2.2525363559367848E-4	0.0	0.0	14.42558070387256	0.0
20	2.2525363559367848E-4	0.0	0.0	14.878565765051448	0.0
21	2.2525363559367848E-4	0.0	0.0	15.359820157497342	0.0
22	2.2525363559367848E-4	0.0	0.0	15.908538013803543	0.0
23	2.2525363559367848E-4	0.0	0.0	16.416484962067287	0.0
24	2.2525363559367848E-4	0.0	0.0	16.8115798388986	0.0
25	2.2525363559367848E-4	0.0	0.0	17.151600201827257	0.0
26	2.2525363559367848E-4	0.0	0.0	17.48463770205251	0.0
27	2.2525363559367848E-4	0.0	0.0	17.85957237849819	0.0
28	2.2525363559367848E-4	0.0	0.0	18.225046402248932	0.0
29	2.2525363559367848E-4	0.0	0.0	18.630502946317552	0.0
30	2.2525363559367848E-4	0.0	0.0	19.19149712576361	0.0
31	2.2525363559367848E-4	0.0	0.0	19.589069792586454	0.0
32	2.2525363559367848E-4	0.0	0.0	19.970649451282142	0.0
33	2.2525363559367848E-4	0.0	0.0	20.356058421782926	0.0
34	2.2525363559367848E-4	0.0	0.0	20.71804101418197	0.0
35	2.2525363559367848E-4	0.0	0.0	21.103675238318345	0.0
36	2.2525363559367848E-4	0.0	0.0	21.486381165192007	0.0
37	2.2525363559367848E-4	0.0	0.0	21.87212801614618	0.0
38	3.378804533905177E-4	0.0	0.0	22.268912295244444	0.0
39	3.378804533905177E-4	0.0	0.0	22.675495107491034	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCG	35	1.2115015E-7	45.000004	1
CGAAACG	35	1.2115015E-7	45.000004	1
ACGCGAG	50	2.1827873E-11	45.0	1
TAATACG	20	7.032419E-4	45.0	1
ACTATCG	20	7.032419E-4	45.0	1
CCGATCA	20	7.032419E-4	45.0	10
TTAGCGT	20	7.032419E-4	45.0	43
TAGAACG	40	6.8121153E-9	45.0	1
TAGCGCG	55	1.8189894E-12	45.0	1
CGGATAA	25	3.8900856E-5	45.0	28
ACGTCTA	20	7.032419E-4	45.0	19
CGTAACG	45	3.8380676E-10	45.0	1
CGGTCTA	25	3.8900856E-5	45.0	31
ATTGCGA	20	7.032419E-4	45.0	29
TATACCG	25	3.8900856E-5	45.0	1
CGATCGA	20	7.032419E-4	45.0	40
CAATACG	50	2.1827873E-11	45.0	1
TCAAACG	25	3.8900856E-5	45.0	1
CTAGACG	30	2.1649412E-6	44.999996	1
CTATCGG	30	2.1649412E-6	44.999996	2
>>END_MODULE