##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	527965
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05929559724603	33.0	31.0	34.0	30.0	34.0
2	32.25702650743894	34.0	31.0	34.0	30.0	34.0
3	32.38559942420426	34.0	31.0	34.0	30.0	34.0
4	35.9413995245897	37.0	35.0	37.0	35.0	37.0
5	35.77513660943434	37.0	35.0	37.0	35.0	37.0
6	35.77630524750694	37.0	35.0	37.0	35.0	37.0
7	35.83789645146932	37.0	35.0	37.0	35.0	37.0
8	35.9977024992187	37.0	35.0	37.0	35.0	37.0
9	37.58992357447937	39.0	37.0	39.0	35.0	39.0
10	37.20260623336774	39.0	37.0	39.0	34.0	39.0
11	37.25855880598146	39.0	37.0	39.0	34.0	39.0
12	37.244631746422584	39.0	37.0	39.0	34.0	39.0
13	37.21141363537356	39.0	37.0	39.0	34.0	39.0
14	38.4682450541229	40.0	38.0	41.0	34.0	41.0
15	38.446705747540086	40.0	38.0	41.0	34.0	41.0
16	38.389720909529984	40.0	38.0	41.0	34.0	41.0
17	38.37864062958719	40.0	38.0	41.0	34.0	41.0
18	38.341515062551494	40.0	38.0	41.0	34.0	41.0
19	38.36649967327379	40.0	38.0	41.0	34.0	41.0
20	38.30260528633527	40.0	38.0	41.0	34.0	41.0
21	38.30425122877463	40.0	38.0	41.0	34.0	41.0
22	38.28894528993399	40.0	38.0	41.0	34.0	41.0
23	38.1978540244145	40.0	38.0	41.0	34.0	41.0
24	38.12373926301933	40.0	37.0	41.0	33.0	41.0
25	38.10615855217675	40.0	37.0	41.0	33.0	41.0
26	38.06991183127669	40.0	37.0	41.0	33.0	41.0
27	38.00609699506596	40.0	37.0	41.0	33.0	41.0
28	37.959107137783754	40.0	37.0	41.0	33.0	41.0
29	37.85093708863277	40.0	37.0	41.0	33.0	41.0
30	37.830558843862754	40.0	37.0	41.0	33.0	41.0
31	37.78403303249269	40.0	37.0	41.0	33.0	41.0
32	37.71864612237554	40.0	37.0	41.0	33.0	41.0
33	37.67411286733022	40.0	37.0	41.0	33.0	41.0
34	37.63103804229447	40.0	37.0	41.0	33.0	41.0
35	37.571971626907086	40.0	37.0	41.0	33.0	41.0
36	37.474675404619624	40.0	36.0	41.0	32.0	41.0
37	37.38755031110017	40.0	36.0	41.0	32.0	41.0
38	37.31632589281487	39.0	36.0	41.0	32.0	41.0
39	37.25727084181717	39.0	36.0	41.0	31.0	41.0
40	37.18509749699317	39.0	36.0	41.0	31.0	41.0
41	37.155737596242176	39.0	36.0	41.0	31.0	41.0
42	37.04982906063849	39.0	36.0	41.0	31.0	41.0
43	36.947259761537225	39.0	35.0	41.0	31.0	41.0
44	36.92101180949495	39.0	35.0	41.0	31.0	41.0
45	36.797732804257855	39.0	35.0	41.0	31.0	41.0
46	36.6765770458269	39.0	35.0	41.0	31.0	41.0
47	36.61260310816058	39.0	35.0	40.0	31.0	41.0
48	36.5736952260093	39.0	35.0	40.0	31.0	41.0
49	36.40803272944229	39.0	35.0	40.0	30.0	41.0
50	36.211474245451875	39.0	35.0	40.0	30.0	41.0
51	35.126330343867494	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	5.0
12	3.0
13	4.0
14	6.0
15	11.0
16	23.0
17	42.0
18	97.0
19	185.0
20	371.0
21	586.0
22	843.0
23	1257.0
24	1620.0
25	1982.0
26	2482.0
27	3289.0
28	4302.0
29	5690.0
30	7741.0
31	9987.0
32	13424.0
33	18115.0
34	26776.0
35	35118.0
36	44785.0
37	66563.0
38	112467.0
39	170141.0
40	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.460882823672023	32.65917248302445	22.438608619889578	26.441336073413957
2	22.91648120614056	30.374361936870812	20.81009157804021	25.899065278948413
3	20.236379305446384	30.20238083963899	21.84747094977887	27.713768905135755
4	21.444603335448374	32.22675745551315	19.456782173060713	26.871857035977765
5	18.400840964836686	35.45386531304158	16.356197854024416	29.789095868097316
6	20.199634445465133	37.628820092240964	18.541948803424468	23.629596658869435
7	87.52038487399733	4.516397867282869	3.9898478118814698	3.9733694468383316
8	89.08507192711637	3.5110281931567435	3.009290388567424	4.394609491159452
9	79.92745731251124	8.85683710094419	6.62809087723618	4.587614709308382
10	26.061954864432302	45.66950460731298	12.39191991893402	15.876620609320694
11	21.459566448533522	26.32977564800697	33.13098406144347	19.079673842016042
12	25.556428929948005	21.846713323799875	32.648186906329016	19.948670839923103
13	25.73674391294877	23.530158249126362	29.979259988825017	20.753837849099845
14	21.657496235545917	26.172757663860292	30.269430738780034	21.900315361813757
15	18.802761546693436	29.945166819770254	29.618630022823485	21.63344161071283
16	22.35167103879992	26.80689060827896	27.857149621660525	22.984288731260595
17	20.155881545178183	26.093206936065837	30.032293807354655	23.718617711401325
18	19.652439082136127	25.782201471688467	30.56793537450399	23.997424071671418
19	20.72088111901357	28.174405500364607	29.476953964751452	21.627759415870372
20	20.410254467625695	27.865672913924218	28.178951256238577	23.54512136221151
21	22.321934219124373	26.877160417830726	29.038856742397694	21.7620486206472
22	20.96976125311337	24.94294129345695	28.266835869801977	25.8204615836277
23	19.85396759254875	27.707707897303795	27.954125746971865	24.484198763175588
24	19.159224569810497	29.135832867709034	28.48995672061595	23.214985841864518
25	20.174064568674062	29.001733069426948	26.804238917352475	24.019963444546512
26	23.250026043393028	27.25559459433864	27.286278446487934	22.208100915780403
27	19.69600257592833	26.093396342560588	27.97969562376294	26.230905457748143
28	20.467076416050308	26.92470144801265	30.303145094845302	22.30507704109174
29	20.965783716723646	26.590777797770688	28.310588770088923	24.13284971541674
30	22.399969694960838	26.530546532440596	28.17724659778584	22.892237174812724
31	23.488678226776397	26.396257327663765	27.145170607900145	22.969893837659694
32	21.50767569819969	27.658651615163883	27.72229219739945	23.11138048923698
33	21.795194757228227	26.041877775988937	28.812894794162492	23.350032672620344
34	20.9574498309547	25.908156790696353	29.381682497892854	23.75271088045609
35	21.60578826247952	25.16625155076568	30.4131902682943	22.814769918460502
36	23.61785345619501	27.191764605608327	28.759671569138106	20.430710369058556
37	22.527440265926717	29.28982034793973	25.757199814381636	22.425539571751916
38	22.160559885598477	27.286089039993183	27.89162160370479	22.661729470703552
39	22.29920543975453	25.598855984771717	26.432244561666018	25.66969401380773
40	22.624416391238057	25.204132849715418	27.383822791283514	24.787627967763015
41	23.325978047787256	22.977659503944388	30.795033761707693	22.901328686560664
42	22.77650980652127	25.77102648849829	27.698237572566363	23.75422613241408
43	20.629966001534193	25.862699231956665	29.58567329273721	23.921661473771934
44	22.479899235744792	24.33968160768233	28.356046328828615	24.824372827744263
45	22.38709005331793	25.023439053725156	29.635676607350863	22.95379428560605
46	20.96048033487068	25.508509086776588	30.412622048810057	23.118388529542678
47	21.176782551873703	26.441525479908705	27.702404515450834	24.679287452766754
48	21.764700311573684	25.000899680850058	29.47468108681447	23.759718920761795
49	22.04615836277026	24.838578314850416	28.72879831049407	24.386465011885257
50	21.543284119212448	24.186830566420124	28.694136921955053	25.57574839241238
51	22.31985074768214	22.796965707954126	29.98172227325675	24.901461271106985
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	911.0
1	741.0
2	571.0
3	432.5
4	294.0
5	256.0
6	218.0
7	264.0
8	310.0
9	338.0
10	366.0
11	384.0
12	402.0
13	454.0
14	506.0
15	555.0
16	604.0
17	569.0
18	534.0
19	811.5
20	1089.0
21	1007.0
22	925.0
23	1688.0
24	2451.0
25	3076.5
26	4227.5
27	4753.0
28	5880.5
29	7008.0
30	9066.5
31	11125.0
32	12009.5
33	12894.0
34	15153.0
35	17412.0
36	18454.0
37	19496.0
38	22513.0
39	25530.0
40	26546.5
41	27563.0
42	29744.0
43	31925.0
44	36865.5
45	41806.0
46	44037.5
47	46269.0
48	48735.5
49	51202.0
50	48690.0
51	46178.0
52	42181.0
53	38184.0
54	33938.0
55	29692.0
56	26296.0
57	22900.0
58	21607.5
59	20315.0
60	19199.0
61	18083.0
62	15766.0
63	13449.0
64	11580.0
65	9711.0
66	8106.5
67	6502.0
68	5638.5
69	4775.0
70	3799.0
71	2823.0
72	2717.5
73	2612.0
74	1952.5
75	1024.0
76	755.0
77	622.5
78	490.0
79	350.5
80	211.0
81	131.0
82	51.0
83	41.0
84	31.0
85	27.0
86	23.0
87	15.5
88	8.0
89	7.5
90	7.0
91	4.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	527965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.478433294598258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03015522120305	19.11650804886775
2	9.9780818387957	5.084517850755971
3	3.5008838506238473	2.67591106980768
4	1.7628932677221747	1.7966303410862312
5	1.0161885677065303	1.294544631852209
6	0.6788082005997661	1.037698167528445
7	0.5135534362299953	0.9159175877738358
8	0.41282534544477445	0.8414514420987333
9	0.36733976267679347	0.8423317475832812
>10	5.005465040788684	32.2338378570965
>50	1.5855341797800164	27.330761059592966
>100	0.1423106336174733	5.178893613760797
>500	0.002980327405601535	0.5186297185878267
>1k	0.002980327405601535	1.1323668636077548
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCTGCT	2042	0.38676806227685545	Illumina Single End Adapter 1 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCC	1490	0.28221567717557033	No Hit
AATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTCT	1247	0.23618989895163506	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGT	1186	0.22463610277196405	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	871	0.16497305692612202	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	677	0.12822819694487322	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	658	0.12462947354464785	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8940649474870493E-4	0.0	0.0	0.48109249666171056	0.0
2	1.8940649474870493E-4	0.0	0.0	0.6555358783252677	0.0
3	1.8940649474870493E-4	0.0	0.0	0.8928622162453951	0.0
4	1.8940649474870493E-4	0.0	0.0	1.9410377581847282	0.0
5	1.8940649474870493E-4	0.0	0.0	2.0976769293419073	0.0
6	1.8940649474870493E-4	0.0	0.0	3.13126817118559	0.0
7	1.8940649474870493E-4	0.0	0.0	3.9288589205723863	0.0
8	1.8940649474870493E-4	0.0	0.0	4.8427452577348875	0.0
9	1.8940649474870493E-4	0.0	0.0	5.912702546570322	0.0
10	1.8940649474870493E-4	0.0	0.0	7.391209644578713	0.0
11	1.8940649474870493E-4	0.0	0.0	8.738079228736753	0.0
12	1.8940649474870493E-4	0.0	0.0	9.336035532658414	0.0
13	1.8940649474870493E-4	0.0	0.0	9.580748723873741	0.0
14	1.8940649474870493E-4	0.0	0.0	9.854251702290872	0.0
15	1.8940649474870493E-4	0.0	0.0	10.076614927125851	0.0
16	1.8940649474870493E-4	0.0	0.0	10.51565918195335	0.0
17	1.8940649474870493E-4	0.0	0.0	11.138048923697594	0.0
18	1.8940649474870493E-4	0.0	0.0	11.980529012339833	0.0
19	1.8940649474870493E-4	0.0	0.0	12.319187824950518	0.0
20	1.8940649474870493E-4	0.0	0.0	12.703872415785137	0.0
21	1.8940649474870493E-4	0.0	0.0	13.108065875578873	0.0
22	1.8940649474870493E-4	0.0	0.0	13.544079626490392	0.0
23	1.8940649474870493E-4	0.0	0.0	13.986343791728618	0.0
24	1.8940649474870493E-4	0.0	0.0	14.30909245878041	0.0
25	1.8940649474870493E-4	0.0	0.0	14.589982290492742	0.0
26	1.8940649474870493E-4	0.0	0.0	14.85477257015143	0.0
27	1.8940649474870493E-4	0.0	0.0	15.17524835926624	0.0
28	1.8940649474870493E-4	0.0	0.0	15.493451270444064	0.0
29	1.8940649474870493E-4	0.0	0.0	15.847641415624143	0.0
30	1.8940649474870493E-4	0.0	0.0	16.343697025371	0.0
31	1.8940649474870493E-4	0.0	0.0	16.65337664428513	0.0
32	1.8940649474870493E-4	0.0	0.0	16.948850776093114	0.0
33	1.8940649474870493E-4	0.0	0.0	17.264023183354958	0.0
34	1.8940649474870493E-4	0.0	0.0	17.573323989279594	0.0
35	1.8940649474870493E-4	0.0	0.0	17.935469207239116	0.0
36	1.8940649474870493E-4	0.0	0.0	18.241550102753024	0.0
37	1.8940649474870493E-4	0.0	0.0	18.53172085270804	0.0
38	1.8940649474870493E-4	0.0	0.0	18.839317000179935	0.0
39	1.8940649474870493E-4	0.0	0.0	19.146534334662334	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAG	30	2.1632623E-6	45.000004	1
CATGACG	30	2.1632623E-6	45.000004	1
CGACAAA	30	2.1632623E-6	45.000004	19
CGAATAT	85	0.0	45.000004	13
CGATCTG	30	2.1632623E-6	45.000004	1
TATCGCG	30	2.1632623E-6	45.000004	1
ATACCCG	30	2.1632623E-6	45.000004	1
TTCTACG	40	6.8030204E-9	45.0	1
GGTACGT	20	7.029818E-4	45.0	8
TGCCCTA	20	7.029818E-4	45.0	33
AGATCGA	20	7.029818E-4	45.0	12
GCGACAT	20	7.029818E-4	45.0	21
GGTAATC	25	3.88793E-5	45.0	8
GATCGAT	25	3.88793E-5	45.0	9
GATCGAC	25	3.88793E-5	45.0	9
AGATACG	25	3.88793E-5	45.0	1
CGAAACG	25	3.88793E-5	45.0	1
CGCGGAC	20	7.029818E-4	45.0	4
CGCATAG	20	7.029818E-4	45.0	1
CGTTGAG	40	6.8030204E-9	45.0	1
>>END_MODULE