##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440669
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.895098134881284	33.0	31.0	34.0	30.0	34.0
2	32.096255919976215	34.0	31.0	34.0	30.0	34.0
3	32.240851977334465	34.0	31.0	34.0	30.0	34.0
4	35.86050527720352	37.0	35.0	37.0	35.0	37.0
5	35.6674533493393	37.0	35.0	37.0	33.0	37.0
6	35.646893700260286	37.0	35.0	37.0	33.0	37.0
7	35.69693352607059	37.0	35.0	37.0	35.0	37.0
8	35.86605365932253	37.0	35.0	37.0	35.0	37.0
9	37.47031672298256	39.0	37.0	39.0	35.0	39.0
10	37.0882567187617	39.0	37.0	39.0	33.0	39.0
11	37.062895279677036	39.0	37.0	39.0	33.0	39.0
12	36.92661385302801	39.0	37.0	39.0	33.0	39.0
13	36.88056568535568	39.0	37.0	39.0	33.0	39.0
14	38.07718264729309	40.0	37.0	41.0	33.0	41.0
15	38.07973785312786	40.0	37.0	41.0	33.0	41.0
16	37.99536840576487	40.0	37.0	41.0	33.0	41.0
17	37.976304210189504	40.0	37.0	41.0	33.0	41.0
18	37.94601390159053	40.0	37.0	41.0	33.0	41.0
19	37.88295977252768	40.0	37.0	41.0	33.0	41.0
20	37.84291384236241	40.0	37.0	41.0	33.0	41.0
21	37.751237323251694	40.0	36.0	41.0	33.0	41.0
22	37.78747540671116	40.0	37.0	41.0	33.0	41.0
23	37.61640596456751	39.0	36.0	41.0	32.0	41.0
24	37.567314696518245	39.0	36.0	41.0	32.0	41.0
25	37.54018322142016	39.0	36.0	41.0	32.0	41.0
26	37.48128413843497	39.0	36.0	41.0	32.0	41.0
27	37.422893373484406	39.0	36.0	41.0	32.0	41.0
28	37.31673659821771	39.0	36.0	41.0	32.0	41.0
29	37.10638370296073	39.0	35.0	41.0	31.0	41.0
30	37.11979739895477	39.0	35.0	41.0	31.0	41.0
31	36.98936162970393	39.0	35.0	41.0	31.0	41.0
32	36.882469608708575	39.0	35.0	41.0	31.0	41.0
33	36.872838797373994	39.0	35.0	41.0	31.0	41.0
34	36.704515180328094	39.0	35.0	40.0	30.0	41.0
35	36.5798388359517	39.0	35.0	40.0	30.0	41.0
36	36.48166991551482	39.0	35.0	40.0	30.0	41.0
37	36.39871422768563	39.0	35.0	40.0	30.0	41.0
38	36.32149981051538	38.0	35.0	40.0	30.0	41.0
39	36.24599415888115	38.0	35.0	40.0	30.0	41.0
40	36.086876998382	38.0	35.0	40.0	30.0	41.0
41	35.97986243643188	38.0	35.0	40.0	29.0	41.0
42	35.879033923420984	38.0	35.0	40.0	29.0	41.0
43	35.7497282540864	38.0	35.0	40.0	29.0	41.0
44	35.58799234799816	38.0	34.0	40.0	28.0	41.0
45	35.30448931057097	37.0	34.0	40.0	28.0	41.0
46	35.087798324819765	37.0	34.0	40.0	26.0	41.0
47	34.973036451395494	37.0	34.0	40.0	26.0	41.0
48	34.8783985258777	37.0	33.0	40.0	26.0	41.0
49	34.69885560363901	37.0	33.0	40.0	26.0	41.0
50	34.545005434918274	37.0	33.0	40.0	26.0	41.0
51	33.28269971339032	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	5.0
12	3.0
13	6.0
14	18.0
15	24.0
16	55.0
17	88.0
18	186.0
19	341.0
20	538.0
21	854.0
22	1216.0
23	1796.0
24	2300.0
25	2819.0
26	3446.0
27	4295.0
28	5330.0
29	6844.0
30	8660.0
31	11206.0
32	14290.0
33	19149.0
34	28648.0
35	36076.0
36	42761.0
37	61160.0
38	89551.0
39	98972.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.247013064227346	30.617992189148772	19.59634101786148	28.5386537287624
2	26.94539438898584	28.2216357402041	18.706557529574354	26.12641234123571
3	20.563052994424385	30.953391320923412	18.535680975970624	29.94787470868157
4	19.757459680621963	33.72485924809778	18.781216740909844	27.736464330370413
5	19.1143465957442	32.91586201888492	17.51949876210943	30.45029262326145
6	20.691267141550686	36.094211301453015	18.664348978484984	24.550172578511308
7	84.37330513378522	4.927281020448455	5.586505971602268	5.112907874164055
8	85.89712459918896	4.559885083815743	4.743923443673142	4.799066873322153
9	78.10941999550684	7.90184923377864	8.924158495378617	5.06457227533591
10	33.18613290247329	36.086495759856035	12.746074718212535	17.981296619458142
11	27.30416707324545	28.43676319414345	25.003347183486923	19.255722549124172
12	24.939580501464818	25.890634467139733	29.16247795964772	20.00730707174773
13	25.27089493474694	26.30523136413045	27.408326884804694	21.01554681631792
14	20.848074178124623	28.1689885151894	29.759524722637625	21.223412584048347
15	19.94535581127785	27.11854021952985	28.37481193367357	24.561292035518722
16	21.162822889742642	29.359905053452817	25.768093512364153	23.709178544440384
17	21.45147491654734	28.257263388166624	26.997587758612475	23.29367393667356
18	26.070361200810588	24.043896893132942	25.701830625707732	24.183911280348745
19	22.26796075966315	29.759524722637625	25.072106274777667	22.900408242921557
20	26.040860600586836	27.92504124410839	24.645255282309396	21.38884287299538
21	23.43573067313562	30.52132099149248	24.3802037356837	21.662744599688203
22	23.945637201618446	24.693137025749483	25.53186178288012	25.829363989751947
23	22.646022297915216	30.63841568161137	23.308424236785434	23.407137783687983
24	19.957609906755412	27.850155105078866	26.823761144986374	25.368473843179345
25	23.42506507151626	28.996366887618596	23.544655966269467	24.03391207459567
26	26.260753536100793	25.580878164790356	24.78322732027894	23.375140978829915
27	23.993292017364507	24.51068715975029	24.018934846789765	27.477085976095438
28	22.93762438474229	28.900830328432452	24.476421077951933	23.685124208873326
29	23.345867306300192	30.0173145830544	23.56349096487386	23.073327145771543
30	27.029584563470543	23.883459013454544	26.544640081330883	22.54231634174403
31	27.75893017207927	25.300622462664723	23.12393202153998	23.816515343716034
32	26.100542584116422	26.074672826997137	25.63851779907368	22.186266789812763
33	24.005999968230125	23.67445860725397	24.89510267343516	27.424438751080743
34	23.769087455664	25.061894528546368	28.6164899278143	22.55252808797533
35	23.74889089089543	27.39516507855102	27.092670462410563	21.76327356814298
36	25.15175789538179	29.137515913304547	25.341015592201856	20.369710599111805
37	23.42120730071777	27.981546239921574	22.208959559215646	26.388286900145008
38	23.960387501730324	30.14484794709862	24.461897705534085	21.432866845636976
39	23.69442824432851	24.711291241271795	23.70645541211204	27.887825102287657
40	27.29213990546192	24.568099866339587	25.292906921067743	22.84685330713075
41	22.255025881103503	24.886933276450122	27.521336876431064	25.336703966015307
42	27.855828297429593	25.287006801022986	24.11492526136397	22.74223964018345
43	23.74979860167155	24.593061912682764	25.915369585788877	25.74176989985681
44	26.903866620978555	23.720524929141828	24.35410705087038	25.02150139900923
45	26.67194651768107	22.41795996541622	27.078147089992715	23.831946426909994
46	23.16228280183085	24.24586254081862	27.230188644992047	25.36166601235848
47	23.38376423120301	25.943735547542484	26.15273595374306	24.519764267511444
48	23.223553279218642	25.764462669259693	28.157415202793935	22.85456884872773
49	26.331101121249734	23.86394323176806	26.478150266980432	23.32680538000177
50	22.081199267477402	24.735345576838853	26.61135682337537	26.572098332308375
51	23.685351136567355	22.125450167813028	27.72557180105703	26.463626894562587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1538.0
1	1251.5
2	965.0
3	720.0
4	475.0
5	450.0
6	425.0
7	449.0
8	473.0
9	550.0
10	627.0
11	663.5
12	700.0
13	715.5
14	731.0
15	747.0
16	763.0
17	711.0
18	659.0
19	759.0
20	859.0
21	968.5
22	1078.0
23	1168.0
24	1258.0
25	1484.5
26	2270.5
27	2830.0
28	3339.0
29	3848.0
30	4746.0
31	5644.0
32	6193.0
33	6742.0
34	7668.0
35	8594.0
36	9686.5
37	10779.0
38	11837.0
39	12895.0
40	14363.5
41	15832.0
42	17590.5
43	19349.0
44	22555.5
45	25762.0
46	29554.5
47	33347.0
48	38075.5
49	42804.0
50	43498.5
51	44193.0
52	41955.5
53	39718.0
54	36515.5
55	33313.0
56	29053.5
57	24794.0
58	22178.5
59	19563.0
60	18364.5
61	17166.0
62	15895.5
63	14625.0
64	13795.0
65	12965.0
66	11251.5
67	9538.0
68	8278.5
69	7019.0
70	6283.5
71	5548.0
72	4838.5
73	4129.0
74	3358.5
75	2445.5
76	2303.0
77	1713.0
78	1123.0
79	975.5
80	828.0
81	530.0
82	232.0
83	212.0
84	192.0
85	147.0
86	102.0
87	63.0
88	24.0
89	15.0
90	6.0
91	5.5
92	5.0
93	3.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	440669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.366769162996682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94933174447588	22.303864934238792
2	9.906055513818023	5.818176911802487
3	3.6121532813407264	3.1823181478348235
4	1.7234879870925959	2.024530954885843
5	1.0657179488238349	1.5648346497985934
6	0.6834470751093283	1.2042379493916542
7	0.5183806431681587	1.065621528053955
8	0.4386401099144407	1.0305154282791096
9	0.32104022322318726	0.848512271468904
>10	4.578053508959234	33.10904999638451
>50	1.0799190114705273	21.037608752996682
>100	0.11990504783519465	5.485385248635147
>500	0.002320742861326348	0.4432198550520655
>1k	0.0015471619075508988	0.8821233711774323
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	2590	0.5877427275347257	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1293	0.2934175083793051	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	724	0.16429565047688854	TruSeq Adapter, Index 15 (95% over 21bp)
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	632	0.1434183026262342	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	595	0.1350219779471667	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	492	0.11164842546219499	No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	466	0.10574830541744484	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	454	0.10302517308909863	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7098298269222477	0.0
2	0.0	0.0	0.0	0.9751083012419752	0.0
3	0.0	0.0	0.0	1.2435637632781067	0.0
4	0.0	0.0	0.0	2.565644508690196	0.0
5	0.0	0.0	0.0	2.7941606965772494	0.0
6	0.0	0.0	0.0	4.257844323063343	0.0
7	0.0	0.0	0.0	5.380001770036014	0.0
8	0.0	0.0	0.0	6.623792461008149	0.0
9	0.0	0.0	0.0	7.720534006249589	0.0
10	0.0	0.0	0.0	9.799418611247898	0.0
11	0.0	0.0	0.0	11.19411621874922	0.0
12	0.0	0.0	0.0	11.992220918648691	0.0
13	0.0	0.0	0.0	12.328754688893477	0.0
14	0.0	0.0	0.0	12.659388339093514	0.0
15	0.0	0.0	0.0	12.959840605987715	0.0
16	0.0	0.0	0.0	13.555752730507479	0.0
17	0.0	0.0	0.0	14.266263340511813	0.0
18	0.0	0.0	0.0	15.259298929582068	0.0
19	0.0	0.0	0.0	15.649614563311692	0.0
20	0.0	0.0	0.0	16.1656481395333	0.0
21	0.0	0.0	0.0	16.639700092359572	0.0
22	0.0	0.0	0.0	17.12237529755894	0.0
23	0.0	0.0	0.0	17.605050502758306	0.0
24	0.0	0.0	0.0	17.949526742294104	0.0
25	0.0	0.0	0.0	18.256786840009166	0.0
26	0.0	0.0	0.0	18.550658203776532	0.0
27	0.0	0.0	0.0	18.92236576659579	0.0
28	0.0	0.0	0.0	19.255949476818202	0.0
29	0.0	0.0	0.0	19.620622281122564	0.0
30	0.0	0.0	0.0	20.286655063097246	0.0
31	0.0	0.0	0.0	20.622281122565916	0.0
32	0.0	0.0	0.0	20.993761757691146	0.0
33	0.0	0.0	0.0	21.36456160973429	0.0
34	0.0	0.0	0.0	21.705860861553685	0.0
35	0.0	0.0	0.0	22.09368029064899	0.0
36	0.0	0.0	0.0	22.3918632806029	0.0
37	0.0	0.0	0.0	22.681876873571774	0.0
38	0.0	0.0	0.0	23.005702692950944	0.0
39	0.0	0.0	0.0	23.38036031579258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1624437E-6	45.000004	1
CTGTCCG	20	7.0285454E-4	45.000004	45
TACCCGG	30	2.1624437E-6	45.000004	2
AAATCCG	30	2.1624437E-6	45.000004	1
CTGTACG	20	7.0285454E-4	45.000004	1
CGAGTGA	20	7.0285454E-4	45.000004	13
CTATCTA	20	7.0285454E-4	45.000004	36
ACACCCG	40	6.7993824E-9	45.000004	1
ATCAGTA	20	7.0285454E-4	45.000004	18
AACCACG	40	6.7993824E-9	45.000004	1
ATTACGA	20	7.0285454E-4	45.000004	20
CGCGCGA	20	7.0285454E-4	45.000004	16
CGACACT	20	7.0285454E-4	45.000004	27
TAGCACG	30	2.1624437E-6	45.000004	1
ATATCGG	20	7.0285454E-4	45.000004	2
TGACCGG	30	2.1624437E-6	45.000004	2
ACGGCTT	40	6.7993824E-9	45.000004	20
CGCTAAG	20	7.0285454E-4	45.000004	1
AACGCAT	20	7.0285454E-4	45.000004	17
GTCCGAC	20	7.0285454E-4	45.000004	40
>>END_MODULE