SRR3548481_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446075
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	2636	0.5909320181583814	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1226	0.27484167460628817	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	746	0.16723645126940537	TruSeq Adapter, Index 15 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	664	0.1488538922826879	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	627	0.14055932298380316	No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	507	0.11365801714958249	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	506	0.11343383960096397	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	496	0.11119206411477892	TruSeq Adapter, Index 15 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.0286385E-4	45.000004	21
ACGCGAA	30	2.1625037E-6	45.000004	28
TTTAGCG	20	7.0286385E-4	45.000004	1
CTCACCG	20	7.0286385E-4	45.000004	1
TCGCACC	20	7.0286385E-4	45.000004	33
GATCGGT	20	7.0286385E-4	45.000004	22
CGAAATA	20	7.0286385E-4	45.000004	43
ATAGGCG	30	2.1625037E-6	45.000004	1
CCCTAAT	20	7.0286385E-4	45.000004	30
ATTACGA	30	2.1625037E-6	45.000004	20
TTCACCG	20	7.0286385E-4	45.000004	45
GTCTAAG	20	7.0286385E-4	45.000004	13
TACGGGT	30	2.1625037E-6	45.000004	4
CGTTCTA	20	7.0286385E-4	45.000004	23
TAACGCG	20	7.0286385E-4	45.000004	1
TATGCGA	20	7.0286385E-4	45.000004	30
CTGACCG	40	6.7993824E-9	45.000004	1
ATCACGG	30	2.1625037E-6	45.000004	2
CCCGTTC	20	7.0286385E-4	45.000004	21
ACTAGGT	20	7.0286385E-4	45.000004	13