##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446075
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87392927198341	33.0	31.0	34.0	30.0	34.0
2	32.06169141960433	34.0	31.0	34.0	30.0	34.0
3	32.212802779801606	34.0	31.0	34.0	30.0	34.0
4	35.80398587681444	37.0	35.0	37.0	35.0	37.0
5	35.63798016028695	37.0	35.0	37.0	33.0	37.0
6	35.625383623830075	37.0	35.0	37.0	33.0	37.0
7	35.70859608810178	37.0	35.0	37.0	35.0	37.0
8	35.87314465056325	37.0	35.0	37.0	35.0	37.0
9	37.46621756431093	39.0	37.0	39.0	35.0	39.0
10	37.069019783668665	39.0	37.0	39.0	33.0	39.0
11	37.054295802275405	39.0	37.0	39.0	33.0	39.0
12	36.929870537465675	39.0	37.0	39.0	33.0	39.0
13	36.86926413719666	39.0	37.0	39.0	33.0	39.0
14	38.06130359244521	40.0	37.0	41.0	33.0	41.0
15	38.07570251639299	40.0	37.0	41.0	33.0	41.0
16	38.02160623213585	40.0	37.0	41.0	33.0	41.0
17	37.960742027685924	40.0	37.0	41.0	33.0	41.0
18	37.9153595247436	40.0	37.0	41.0	33.0	41.0
19	37.857221319284875	40.0	37.0	41.0	33.0	41.0
20	37.808422350501594	40.0	37.0	41.0	33.0	41.0
21	37.72220142352744	40.0	36.0	41.0	33.0	41.0
22	37.747957182088214	40.0	36.0	41.0	33.0	41.0
23	37.59267836126212	39.0	36.0	41.0	32.0	41.0
24	37.55778512581965	39.0	36.0	41.0	32.0	41.0
25	37.54096732612229	39.0	36.0	41.0	32.0	41.0
26	37.46629378467746	39.0	36.0	41.0	32.0	41.0
27	37.39267387771115	39.0	36.0	41.0	32.0	41.0
28	37.295638625791625	39.0	36.0	41.0	32.0	41.0
29	37.076505072017035	39.0	35.0	41.0	31.0	41.0
30	37.1053791402791	39.0	35.0	41.0	31.0	41.0
31	36.978960937062155	39.0	35.0	41.0	31.0	41.0
32	36.8672936165443	39.0	35.0	40.0	31.0	41.0
33	36.84252872274842	39.0	35.0	41.0	31.0	41.0
34	36.67266042705823	39.0	35.0	40.0	30.0	41.0
35	36.56127557025164	39.0	35.0	40.0	30.0	41.0
36	36.49465000280222	39.0	35.0	40.0	30.0	41.0
37	36.38572661547946	39.0	35.0	40.0	30.0	41.0
38	36.27811242504063	38.0	35.0	40.0	30.0	41.0
39	36.20189205851034	38.0	35.0	40.0	30.0	41.0
40	36.06509667656784	38.0	35.0	40.0	30.0	41.0
41	35.98743709017542	38.0	35.0	40.0	29.0	41.0
42	35.87013170430981	38.0	35.0	40.0	29.0	41.0
43	35.737683125035026	38.0	35.0	40.0	29.0	41.0
44	35.64132488931234	38.0	34.0	40.0	29.0	41.0
45	35.34549571260438	37.0	34.0	40.0	28.0	41.0
46	35.110730258364626	37.0	34.0	40.0	27.0	41.0
47	34.98187748696968	37.0	34.0	40.0	26.0	41.0
48	34.911131536176654	37.0	34.0	40.0	26.0	41.0
49	34.74908479515776	37.0	33.0	40.0	26.0	41.0
50	34.56134282351623	36.0	33.0	40.0	26.0	41.0
51	33.35074819256852	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	1.0
11	4.0
12	5.0
13	8.0
14	7.0
15	17.0
16	44.0
17	90.0
18	187.0
19	326.0
20	546.0
21	839.0
22	1320.0
23	1749.0
24	2289.0
25	2897.0
26	3461.0
27	4360.0
28	5483.0
29	7024.0
30	8892.0
31	11549.0
32	14419.0
33	19220.0
34	29106.0
35	36569.0
36	43436.0
37	61813.0
38	91098.0
39	99284.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.521941377571036	30.51953146892339	19.388443647368717	28.57008350613686
2	26.8746287059351	28.05828616264081	18.759625623493807	26.307459507930282
3	20.55685703076837	31.00532421677969	18.48612901417923	29.951689738272712
4	19.90248276635095	33.79028190326739	18.736311158437484	27.570924171944178
5	19.301462758504737	32.767359748921145	17.51566440620972	30.4155130863644
6	20.57322199181752	36.11029535392031	18.64955444712212	24.666928207140053
7	84.44162977077846	4.963963459059576	5.508490724653925	5.085916045508043
8	85.97791851146108	4.564030712324161	4.728128677912907	4.729922098301855
9	78.26239982065796	7.756319004651684	8.840665807319397	5.140615367370958
10	32.94759849801042	36.253096452390295	12.842907582805582	17.9563974667937
11	27.361542341534495	28.49879504567617	24.948943563302134	19.190719049487193
12	24.836630611444264	25.868968222832482	29.375329260774535	19.91907190494872
13	25.322647536849185	26.386370005043997	27.250574454968334	21.040408003138484
14	20.7438211063162	28.33424872499019	29.7492574118702	21.172672756823406
15	20.153337443255058	27.102168917782883	28.154906686095387	24.58958695286667
16	21.128733957294177	29.457378243568904	25.74723981393263	23.666647985204282
17	21.43294289076949	28.122400941545706	27.167180406882252	23.277475760802556
18	26.240878775990584	23.898447570475817	25.72302863868184	24.137645014851763
19	22.130807599618898	29.708457098021633	25.19845317491453	22.962282127444936
20	26.242896373928147	27.901810233705092	24.52973154738553	21.325561844981227
21	23.45681779969736	30.452278204337834	24.488931233536963	21.60197276242784
22	23.941041304713334	24.644510452278205	25.628201535616206	25.78624670739226
23	22.50249397522838	30.643950008406655	23.397859104410692	23.45569691195427
24	19.902258588802333	27.849352687328366	26.89592557305386	25.352463150815446
25	23.36243905172897	29.04287395617329	23.752059631227933	23.84262736086981
26	26.308356218124757	25.59816174410133	24.734181471725606	23.35930056604831
27	23.917951017205628	24.64226867679202	23.97287451661716	27.466905789385194
28	22.890545311887013	28.905004763772908	24.504399484391637	23.700050439948438
29	23.369164378187524	30.12609987109791	23.573165947430365	22.9315698032842
30	26.9909768536681	23.793084122625118	26.624446561676844	22.591492462029926
31	27.829625063049935	25.47150142913187	22.954435913243287	23.7444375945749
32	25.97478002578042	25.952138093369946	25.857086812755703	22.21599506809393
33	23.99686151431934	23.710586784733508	24.972706383455698	27.319845317491453
34	23.46780249957967	25.00722972594295	28.883707896654148	22.641259877823234
35	23.75587064955445	27.317379364456652	27.21336098189766	21.71338900409124
36	25.065515888583757	29.058790562125203	25.316370565487865	20.55932298380317
37	23.3846326290422	27.88477274001009	22.179454127669114	26.551140503278596
38	23.908983915260887	30.2532085411646	24.505968727231966	21.331838816342543
39	23.724709970296477	24.797399540436025	23.67920192792692	27.798688561340583
40	27.478787199461973	24.572997814268902	25.210558762539932	22.737656223729193
41	22.207700498795045	24.78596648545648	27.498066468643167	25.508266547105308
42	27.918175194754248	25.143978030600234	24.068374152328644	22.869472622316874
43	23.747576080255563	24.471893739841953	26.077901698144927	25.70262848175755
44	27.037605783780755	23.69085916045508	24.319453006781373	24.952082048982792
45	26.79616656391862	22.47402342655383	27.098806254553608	23.63100375497394
46	23.139158213304935	24.182704702124084	27.28733957294177	25.39079751162921
47	23.44179790393992	26.03867062713669	26.27764389396402	24.241887574959367
48	23.29787591772684	25.87233088606176	28.205794989631787	22.62399820657961
49	26.363279717536287	23.7807543574511	26.437706663677634	23.418259261334978
50	22.099871097909542	24.73664742476041	26.6674886510116	26.495992826318442
51	23.800930336826767	22.14717256066805	27.585495712604384	26.466401389900803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1528.0
1	1252.5
2	977.0
3	697.5
4	418.0
5	431.5
6	445.0
7	468.5
8	492.0
9	534.0
10	576.0
11	663.0
12	750.0
13	759.0
14	768.0
15	781.5
16	795.0
17	717.0
18	639.0
19	738.0
20	837.0
21	980.0
22	1123.0
23	1201.0
24	1279.0
25	1541.5
26	2381.0
27	2958.0
28	3482.0
29	4006.0
30	4856.0
31	5706.0
32	6235.5
33	6765.0
34	7785.5
35	8806.0
36	9795.0
37	10784.0
38	11975.0
39	13166.0
40	14728.5
41	16291.0
42	17857.5
43	19424.0
44	22660.0
45	25896.0
46	29835.0
47	33774.0
48	38486.5
49	43199.0
50	43966.0
51	44733.0
52	42413.5
53	40094.0
54	37086.5
55	34079.0
56	29664.5
57	25250.0
58	22411.0
59	19572.0
60	18326.0
61	17080.0
62	15853.0
63	14626.0
64	13864.5
65	13103.0
66	11373.5
67	9644.0
68	8416.5
69	7189.0
70	6439.0
71	5689.0
72	4986.0
73	4283.0
74	3431.5
75	2548.0
76	2516.0
77	1797.5
78	1079.0
79	947.5
80	816.0
81	518.0
82	220.0
83	217.0
84	214.0
85	145.0
86	76.0
87	45.0
88	14.0
89	9.0
90	4.0
91	2.5
92	1.0
93	2.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.009603819031764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.87481996754285	22.01098467098779
2	9.876650129650185	5.730354146406812
3	3.62818097632362	3.157562781206883
4	1.7645908771565613	2.0476032899595835
5	1.0495213896863622	1.522309985720051
6	0.731265136553222	1.272822713884748
7	0.5397182437117264	1.095990869678657
8	0.4188740473866236	0.9721096131808231
9	0.30504833777568435	0.7964398282074104
>10	4.567152024167073	32.709324379040225
>50	1.1133349191825104	21.592095408302526
>100	0.1254243789343301	5.539970722114402
>500	0.003871122806615126	0.6850284076238111
>1k	0.0015484491226460506	0.8674031836862816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	2636	0.5909320181583814	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1226	0.27484167460628817	No Hit
AAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	746	0.16723645126940537	TruSeq Adapter, Index 15 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	664	0.1488538922826879	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	627	0.14055932298380316	No Hit
CGCAGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	507	0.11365801714958249	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	506	0.11343383960096397	No Hit
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	496	0.11119206411477892	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2417754861850587E-4	0.0	0.0	0.6884492518074314	0.0
2	2.2417754861850587E-4	0.0	0.0	0.9532029367258869	0.0
3	2.2417754861850587E-4	0.0	0.0	1.24082273160343	0.0
4	2.2417754861850587E-4	0.0	0.0	2.547777840049319	0.0
5	2.2417754861850587E-4	0.0	0.0	2.7676960152440735	0.0
6	2.2417754861850587E-4	0.0	0.0	4.176427730762764	0.0
7	2.2417754861850587E-4	0.0	0.0	5.304040800313849	0.0
8	2.2417754861850587E-4	0.0	0.0	6.512357787367595	0.0
9	2.2417754861850587E-4	0.0	0.0	7.624278428515384	0.0
10	2.2417754861850587E-4	0.0	0.0	9.659138037325562	0.0
11	2.2417754861850587E-4	0.0	0.0	11.06988735078182	0.0
12	2.2417754861850587E-4	0.0	0.0	11.839713052737768	0.0
13	4.4835509723701174E-4	0.0	0.0	12.183825589867174	0.0
14	4.4835509723701174E-4	0.0	0.0	12.519195202600459	0.0
15	4.4835509723701174E-4	0.0	0.0	12.811298548450372	0.0
16	4.4835509723701174E-4	0.0	0.0	13.436081376450149	0.0
17	4.4835509723701174E-4	0.0	0.0	14.157036372807264	0.0
18	4.4835509723701174E-4	0.0	0.0	15.170094715014292	0.0
19	4.4835509723701174E-4	0.0	0.0	15.546488819144763	0.0
20	4.4835509723701174E-4	0.0	0.0	16.060079583029758	0.0
21	4.4835509723701174E-4	0.0	0.0	16.51134898839881	0.0
22	4.4835509723701174E-4	0.0	0.0	16.995572493414784	0.0
23	6.725326458555176E-4	0.0	0.0	17.45580900072858	0.0
24	6.725326458555176E-4	0.0	0.0	17.805077621476208	0.0
25	6.725326458555176E-4	0.0	0.0	18.11220086308356	0.0
26	6.725326458555176E-4	0.0	0.0	18.40789104971137	0.0
27	6.725326458555176E-4	0.0	0.0	18.789665415008688	0.0
28	6.725326458555176E-4	0.0	0.0	19.133553774589476	0.0
29	6.725326458555176E-4	0.0	0.0	19.501204954323825	0.0
30	6.725326458555176E-4	0.0	0.0	20.150423135123017	0.0
31	6.725326458555176E-4	0.0	0.0	20.473014627585048	0.0
32	6.725326458555176E-4	0.0	0.0	20.836406433895647	0.0
33	6.725326458555176E-4	0.0	0.0	21.20204001569243	0.0
34	6.725326458555176E-4	0.0	0.0	21.510059967494257	0.0
35	6.725326458555176E-4	0.0	0.0	21.89519699602085	0.0
36	6.725326458555176E-4	0.0	0.0	22.209269741635374	0.0
37	6.725326458555176E-4	0.0	0.0	22.534775542229447	0.0
38	6.725326458555176E-4	0.0	0.0	22.86723084683069	0.0
39	6.725326458555176E-4	0.0	0.0	23.242952418315305	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGG	20	7.0286385E-4	45.000004	21
ACGCGAA	30	2.1625037E-6	45.000004	28
TTTAGCG	20	7.0286385E-4	45.000004	1
CTCACCG	20	7.0286385E-4	45.000004	1
TCGCACC	20	7.0286385E-4	45.000004	33
GATCGGT	20	7.0286385E-4	45.000004	22
CGAAATA	20	7.0286385E-4	45.000004	43
ATAGGCG	30	2.1625037E-6	45.000004	1
CCCTAAT	20	7.0286385E-4	45.000004	30
ATTACGA	30	2.1625037E-6	45.000004	20
TTCACCG	20	7.0286385E-4	45.000004	45
GTCTAAG	20	7.0286385E-4	45.000004	13
TACGGGT	30	2.1625037E-6	45.000004	4
CGTTCTA	20	7.0286385E-4	45.000004	23
TAACGCG	20	7.0286385E-4	45.000004	1
TATGCGA	20	7.0286385E-4	45.000004	30
CTGACCG	40	6.7993824E-9	45.000004	1
ATCACGG	30	2.1625037E-6	45.000004	2
CCCGTTC	20	7.0286385E-4	45.000004	21
ACTAGGT	20	7.0286385E-4	45.000004	13
>>END_MODULE