FastQCFastQC Report
Sat 18 Jun 2016
SRR3548480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195346
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC157461.3172755001480743No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT155831.3036392810115232No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGT155331.2994563917058324No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT64340.5382541958562626Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT41450.34676152344174827No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT32450.27146951593931795No Hit
AACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG29140.24377878873564643No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTTC23210.19416972157015625No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT23110.19333314370901813No Hit
AATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC13470.11268703789530396No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG253.890899E-545.0000041
TAACGCG1000.045.0000041
TCGATAG253.890899E-545.0000041
AACGCGC253.890899E-545.00000419
CGTCGCA253.890899E-545.00000419
ATACGTC253.890899E-545.0000045
TATCGTC253.890899E-545.00000431
AACCGAT207.0334005E-445.036
CCGGGTA1050.045.05
ATCGTAG207.0334005E-445.01
GTAATCG207.0334005E-445.026
TCAATCG207.0334005E-445.01
TACGCCC207.0334005E-445.029
TCTACCG207.0334005E-445.01
CTACGGT207.0334005E-445.027
TCGCTTA207.0334005E-445.023
GCCTCGT207.0334005E-445.030
CTCGCAC207.0334005E-445.030
TCGTACC207.0334005E-445.016
CCGACGT207.0334005E-445.043