##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548480_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1195346
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932365189660565	33.0	31.0	34.0	30.0	34.0
2	32.13237087839002	34.0	31.0	34.0	30.0	34.0
3	32.24816998592876	34.0	31.0	34.0	30.0	34.0
4	35.84204740719424	37.0	35.0	37.0	35.0	37.0
5	35.63571718983457	37.0	35.0	37.0	33.0	37.0
6	35.63325681434497	37.0	35.0	37.0	33.0	37.0
7	35.57442782257188	37.0	35.0	37.0	35.0	37.0
8	35.869867803966386	37.0	35.0	37.0	35.0	37.0
9	37.51589079647232	39.0	37.0	39.0	35.0	39.0
10	37.06232421407693	39.0	37.0	39.0	33.0	39.0
11	37.02433855971409	39.0	37.0	39.0	33.0	39.0
12	36.908674141210994	39.0	37.0	39.0	33.0	39.0
13	36.925431632347454	39.0	37.0	39.0	33.0	39.0
14	38.06911889946509	40.0	37.0	41.0	33.0	41.0
15	38.12243400655542	40.0	37.0	41.0	33.0	41.0
16	37.951219981494894	40.0	37.0	41.0	33.0	41.0
17	37.96905247518292	40.0	37.0	41.0	33.0	41.0
18	37.886995899095325	40.0	37.0	41.0	33.0	41.0
19	37.86759231218409	40.0	37.0	41.0	33.0	41.0
20	37.91595153202504	40.0	37.0	41.0	33.0	41.0
21	37.921207750726566	40.0	37.0	41.0	33.0	41.0
22	37.93695214607319	40.0	37.0	41.0	33.0	41.0
23	37.81867593148762	40.0	37.0	41.0	33.0	41.0
24	37.750393609883666	40.0	37.0	41.0	33.0	41.0
25	37.7401999086457	40.0	37.0	41.0	33.0	41.0
26	37.67139807218998	40.0	37.0	41.0	33.0	41.0
27	37.62855273703179	40.0	36.0	41.0	33.0	41.0
28	37.535556232254095	40.0	36.0	41.0	32.0	41.0
29	37.4595983087742	40.0	36.0	41.0	32.0	41.0
30	37.520443453192634	40.0	36.0	41.0	32.0	41.0
31	37.43555840735653	40.0	36.0	41.0	32.0	41.0
32	37.39132937241602	39.0	36.0	41.0	32.0	41.0
33	37.32678906358494	39.0	36.0	41.0	32.0	41.0
34	37.301082699067884	39.0	36.0	41.0	32.0	41.0
35	37.19879516056439	39.0	36.0	41.0	31.0	41.0
36	37.22602995283374	39.0	36.0	41.0	32.0	41.0
37	37.2034641016074	39.0	36.0	41.0	32.0	41.0
38	37.18105803675254	39.0	36.0	41.0	32.0	41.0
39	37.09319812004223	39.0	35.0	41.0	31.0	41.0
40	36.90857793475696	39.0	35.0	41.0	31.0	41.0
41	36.89821942768035	39.0	35.0	41.0	31.0	41.0
42	36.81590769534511	39.0	35.0	40.0	31.0	41.0
43	36.81614528345768	39.0	35.0	40.0	31.0	41.0
44	36.70866343301437	39.0	35.0	40.0	31.0	41.0
45	36.53548846944734	39.0	35.0	40.0	30.0	41.0
46	36.40231112999918	39.0	35.0	40.0	30.0	41.0
47	36.31324403143525	38.0	35.0	40.0	30.0	41.0
48	36.289262690467865	38.0	35.0	40.0	30.0	41.0
49	36.134364443433114	38.0	35.0	40.0	30.0	41.0
50	36.001920782769176	38.0	35.0	40.0	29.0	41.0
51	34.670302991769745	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	7.0
11	0.0
12	4.0
13	22.0
14	34.0
15	48.0
16	78.0
17	151.0
18	274.0
19	584.0
20	1151.0
21	1921.0
22	2771.0
23	3516.0
24	4307.0
25	5331.0
26	6519.0
27	8157.0
28	10715.0
29	14350.0
30	19369.0
31	25709.0
32	35366.0
33	47965.0
34	71566.0
35	88701.0
36	104979.0
37	151806.0
38	251419.0
39	338435.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.832461898061315	35.12472539331708	18.384886049729534	25.657926658892073
2	22.914118589931284	33.97317596746047	18.17607621558946	24.936629227018788
3	19.790169540869336	28.612636006645776	25.271344029260145	26.32585042322474
4	24.63094367655892	29.42855039461378	18.295539534159982	27.64496639466732
5	18.67559685647503	34.98175423684858	17.67647191691778	28.66617698975861
6	21.586469524305095	34.16876787139456	22.56568391076726	21.679078693533086
7	82.43295246731908	7.258065865448163	5.884237701887153	4.424743965345599
8	83.73985440198905	3.090820565760876	4.163313383739938	9.006011648510139
9	78.7703309334703	7.225857617794346	8.421912985863507	5.5818984628718376
10	32.43378904518022	38.96035122884922	13.601668470886255	15.0041912550843
11	25.237880914814625	26.6629913012634	28.651034930472015	19.448092853449964
12	26.304015741049035	23.234527910747182	32.53066476150002	17.930791586703766
13	26.67311389338317	26.263692688142175	26.41427670314704	20.648916715327612
14	20.530959236907137	26.693359077622713	31.738425527002224	21.037256158467923
15	19.665435781773645	28.851478986000707	28.57256392709726	22.91052130512839
16	22.287103483008266	27.904472847192363	29.982364938687205	19.826058731112166
17	21.49436230179379	26.876987918142532	28.879420686562717	22.74922909350096
18	22.037301333672428	26.668345399574683	26.555323730534923	24.739029536217966
19	22.60567233252966	28.380903939110517	29.26006361338056	19.753360114979262
20	25.21328552569716	29.640873855770632	26.335220095269484	18.81062052326272
21	23.316847172283172	27.01435400294141	26.681981618711237	22.986817206064185
22	22.437603840227013	28.11386828583523	26.56352219357408	22.885005680363676
23	22.67845460644868	27.34388202244371	25.830763645003202	24.146899726104408
24	20.31805017124749	30.300515499278035	26.592216814211117	22.789217515263363
25	22.03939277832527	27.479240320375858	26.385916713654456	24.095450187644417
26	24.944158427769032	28.711686825404527	24.444888760241803	21.899265986584638
27	20.937870708564716	26.855738840469623	28.203298459190897	24.003091991774767
28	22.645493438719836	29.624225956333984	25.64102778609708	22.089252818849104
29	24.04818353849011	28.46882827231613	25.845152784214783	21.63783540497898
30	24.070185536238043	24.505624312960432	25.555027581972084	25.869162568829445
31	25.30999392644473	27.211200773667205	25.444013699799058	22.03479160008901
32	23.19479046234312	25.437404734696063	25.352492081790544	26.015312721170268
33	23.92520659290281	26.71444083972339	27.480662502739793	21.879690064634005
34	24.44815141390024	25.677920869773267	27.03150384909474	22.842423867231748
35	21.393889300671106	25.59484868816226	29.649908896670922	23.36135311449572
36	23.248498761028188	26.762962355669405	28.9180705837473	21.07046829955511
37	21.960587143806062	27.129634432206238	24.90684705516227	26.002931368825426
38	22.724968335527958	25.808594331683043	30.146836146186963	21.31960118660204
39	23.629058030059916	23.55677770285758	27.40687633538741	25.407287931695087
40	24.510978411271715	24.812732045784234	29.47673727941533	21.199552263528716
41	22.297560706272492	23.938591838681017	31.589096378789073	22.17475107625742
42	24.48320402628193	26.299247247240547	26.823279619457463	22.394269107020058
43	23.625962691973704	24.26853814711389	28.536842052426664	23.568657108485745
44	23.607725294600893	23.82080167583277	27.235796162784666	25.33567686678167
45	25.69256098234319	24.773831175241313	27.48325589410932	22.050351948306183
46	20.262836032412373	24.28326191746992	31.05828772589694	24.39561432422077
47	22.369422744544256	26.43778454104502	27.016027158663686	24.17676555574704
48	22.3799636255946	23.781900805289848	31.337872047089295	22.50026352202626
49	23.17479625146192	23.450783287851383	28.68742606743152	24.686994393255173
50	20.761436437650687	24.435686403769285	27.306403334264722	27.496473824315302
51	19.989609702964664	22.954525300624255	31.273371893995545	25.782493102415533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1475.0
1	1241.0
2	1007.0
3	729.0
4	451.0
5	448.5
6	446.0
7	476.5
8	507.0
9	577.5
10	648.0
11	736.5
12	825.0
13	917.5
14	1010.0
15	1091.5
16	1173.0
17	1190.5
18	1208.0
19	1549.5
20	1891.0
21	2354.0
22	2817.0
23	4179.0
24	5541.0
25	6251.5
26	8521.5
27	10081.0
28	11582.0
29	13083.0
30	15985.0
31	18887.0
32	23187.0
33	27487.0
34	30285.5
35	33084.0
36	36059.0
37	39034.0
38	42268.0
39	45502.0
40	49168.5
41	52835.0
42	59026.0
43	65217.0
44	90819.5
45	116422.0
46	115323.5
47	114225.0
48	112790.5
49	111356.0
50	106663.5
51	101971.0
52	96353.0
53	90735.0
54	81152.5
55	71570.0
56	65219.5
57	58869.0
58	53715.0
59	48561.0
60	47897.5
61	47234.0
62	42847.5
63	38461.0
64	30812.0
65	23163.0
66	19212.5
67	15262.0
68	11785.0
69	8308.0
70	7511.5
71	6715.0
72	5400.0
73	4085.0
74	3786.5
75	2701.0
76	1914.0
77	1401.5
78	889.0
79	672.5
80	456.0
81	304.5
82	153.0
83	156.5
84	160.0
85	123.5
86	87.0
87	66.5
88	46.0
89	35.0
90	24.0
91	18.5
92	13.0
93	8.0
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1195346.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.424660262994045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31625113042763	16.44092023356193
2	11.073916694961385	4.9665763933041385
3	4.076440167902628	2.742383575429165
4	1.9795628738192164	1.7756409965852828
5	1.1646500704024374	1.3058441077023377
6	0.7600025733354066	1.0225679703628625
7	0.5433247579573867	0.8528711176767506
8	0.38632914098628895	0.6930639789049486
9	0.3027208106686317	0.6109570200403988
>10	4.313862399964571	24.031459953050206
>50	1.6372383795253398	26.17145860081274
>100	0.43669693944199006	12.664151627138914
>500	0.004126861111565183	0.5965687848803681
>1k	0.003376522727644241	1.6417209516507547
>5k	3.7516919196047124E-4	0.541295101102847
>10k+	0.0011255075758814137	3.9425195877963346
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCC	15746	1.3172755001480743	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCT	15583	1.3036392810115232	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGT	15533	1.2994563917058324	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	6434	0.5382541958562626	Illumina Single End Adapter 1 (95% over 21bp)
AATGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	4145	0.34676152344174827	No Hit
AATGATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCT	3245	0.27146951593931795	No Hit
AACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTG	2914	0.24377878873564643	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTTC	2321	0.19416972157015625	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTAT	2311	0.19333314370901813	No Hit
AATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1347	0.11268703789530396	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.509733583414342E-4	0.0	0.0	0.6008302198693934	0.0
2	2.509733583414342E-4	0.0	0.0	0.821937748568197	0.0
3	2.509733583414342E-4	0.0	0.0	1.2874933282915575	0.0
4	2.509733583414342E-4	0.0	0.0	3.54064848169484	0.0
5	2.509733583414342E-4	0.0	0.0	3.8153806512925965	0.0
6	2.509733583414342E-4	0.0	0.0	5.123035506037582	0.0
7	2.509733583414342E-4	0.0	0.0	6.067364595690285	0.0
8	2.509733583414342E-4	0.0	0.0	6.736375911242435	0.0
9	2.509733583414342E-4	0.0	0.0	8.923441413615807	0.0
10	2.509733583414342E-4	0.0	0.0	10.2499192702364	0.0
11	3.346311444552456E-4	0.0	0.0	12.715230569224309	0.0
12	3.346311444552456E-4	0.0	0.0	13.323171700913376	0.0
13	3.346311444552456E-4	0.0	0.0	13.653954587207386	0.0
14	3.346311444552456E-4	0.0	0.0	14.13824951102024	0.0
15	3.346311444552456E-4	0.0	0.0	14.43280857592697	0.0
16	3.346311444552456E-4	0.0	0.0	14.941196942140602	0.0
17	3.346311444552456E-4	0.0	0.0	15.527387049440078	0.0
18	3.346311444552456E-4	0.0	0.0	16.53186608730861	0.0
19	3.346311444552456E-4	0.0	0.0	16.917193850148827	0.0
20	3.346311444552456E-4	0.0	0.0	17.320842668147968	0.0
21	3.346311444552456E-4	0.0	0.0	17.756030471512013	0.0
22	3.346311444552456E-4	0.0	0.0	18.221920682379828	0.0
23	3.346311444552456E-4	0.0	0.0	18.69098988911997	0.0
24	3.346311444552456E-4	0.0	0.0	19.020936197552842	0.0
25	3.346311444552456E-4	0.0	0.0	19.321267649701426	0.0
26	3.346311444552456E-4	0.0	0.0	19.605118517985588	0.0
27	3.346311444552456E-4	0.0	0.0	19.920926660565225	0.0
28	3.346311444552456E-4	0.0	0.0	20.237822354364344	0.0
29	3.346311444552456E-4	0.0	0.0	20.563836746849866	0.0
30	3.346311444552456E-4	0.0	0.0	21.08385354533332	0.0
31	4.18288930569057E-4	0.0	0.0	21.420994423371976	0.0
32	4.18288930569057E-4	0.0	0.0	21.75353412317438	0.0
33	4.18288930569057E-4	0.0	0.0	22.06967689689847	0.0
34	4.18288930569057E-4	0.0	0.0	22.391508400078305	0.0
35	4.18288930569057E-4	0.0	0.0	22.71961423721667	0.0
36	4.18288930569057E-4	0.0	0.0	23.04947688786343	0.0
37	4.18288930569057E-4	0.0	0.0	23.37565859592118	0.0
38	4.18288930569057E-4	0.0	0.0	23.705019299851255	0.0
39	4.18288930569057E-4	0.0	0.0	24.046343067195608	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.890899E-5	45.000004	1
TAACGCG	100	0.0	45.000004	1
TCGATAG	25	3.890899E-5	45.000004	1
AACGCGC	25	3.890899E-5	45.000004	19
CGTCGCA	25	3.890899E-5	45.000004	19
ATACGTC	25	3.890899E-5	45.000004	5
TATCGTC	25	3.890899E-5	45.000004	31
AACCGAT	20	7.0334005E-4	45.0	36
CCGGGTA	105	0.0	45.0	5
ATCGTAG	20	7.0334005E-4	45.0	1
GTAATCG	20	7.0334005E-4	45.0	26
TCAATCG	20	7.0334005E-4	45.0	1
TACGCCC	20	7.0334005E-4	45.0	29
TCTACCG	20	7.0334005E-4	45.0	1
CTACGGT	20	7.0334005E-4	45.0	27
TCGCTTA	20	7.0334005E-4	45.0	23
GCCTCGT	20	7.0334005E-4	45.0	30
CTCGCAC	20	7.0334005E-4	45.0	30
TCGTACC	20	7.0334005E-4	45.0	16
CCGACGT	20	7.0334005E-4	45.0	43
>>END_MODULE