SRR3548477_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281476
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	7174	2.548707527462377	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT	6545	2.3252426494621212	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	6313	2.2428199917577345	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	6061	2.1532919325271074	TruSeq Adapter, Index 20 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1774	0.6302491153775099	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	1701	0.6043143998067331	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	1675	0.5950773778226208	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	1545	0.5488922679020591	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	1382	0.4909832454632011	No Hit
AACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	1268	0.45048245676363174	TruSeq Adapter, Index 23 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTAT	1051	0.37338885020392504	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACTC	927	0.3293353607412355	No Hit
ACCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTC	901	0.3200983387571232	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	847	0.30091375463627446	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	801	0.2845713311259219	TruSeq Adapter, Index 23 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	545	0.19362219159004676	No Hit
ACCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTAC	536	0.19042476090323862	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	528	0.18758260029274254	No Hit
ACCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	470	0.16697693586664583	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	466	0.16555585556139776	TruSeq Adapter, Index 20 (95% over 21bp)
GAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	435	0.1545424831957254	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	410	0.1456607312879251	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	401	0.14246330060111698	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	358	0.12718668731970043	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	334	0.11866020548821213	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATG	313	0.11119953388565988	No Hit
AATGATCCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT	307	0.10906791342778781	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	299	0.10622575281729171	No Hit
GAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	292	0.10373886228310762	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	289	0.10267305205417158	TruSeq Adapter, Index 23 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAC	25	3.8832753E-5	45.000004	32
TAATAGG	50	2.1827873E-11	45.000004	2
AATCCGG	25	3.8832753E-5	45.000004	2
TACCCGG	25	3.8832753E-5	45.000004	2
ACAAAGT	25	3.8832753E-5	45.000004	12
CGAACAG	25	3.8832753E-5	45.000004	1
CCGGCGA	35	1.2075543E-7	45.000004	7
CTTCACT	25	3.8832753E-5	45.000004	13
GGCAAAC	25	3.8832753E-5	45.000004	8
ATATGGG	95	0.0	45.000004	3
TACGGGC	70	0.0	45.000004	4
AATATGG	35	1.2075543E-7	45.000004	2
CACCTGG	35	1.2075543E-7	45.000004	2
TAGTTAT	25	3.8832753E-5	45.000004	27
GTTACTA	25	3.8832753E-5	45.000004	44
CTGCGCG	35	1.2075543E-7	45.000004	1
ATTCAGT	25	3.8832753E-5	45.000004	28
AACATAG	25	3.8832753E-5	45.000004	1
GGGCGTA	25	3.8832753E-5	45.000004	7
TGCGAAG	35	1.2075543E-7	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content