##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281476
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.282393525558128	31.0	31.0	34.0	28.0	34.0
2	31.48648552629709	31.0	31.0	34.0	30.0	34.0
3	31.641091958106554	31.0	31.0	34.0	30.0	34.0
4	35.30903167588001	37.0	35.0	37.0	33.0	37.0
5	35.08015603461752	37.0	35.0	37.0	32.0	37.0
6	35.050018474043966	36.0	35.0	37.0	32.0	37.0
7	34.851855930878656	36.0	35.0	37.0	32.0	37.0
8	35.30512725774133	37.0	35.0	37.0	33.0	37.0
9	36.73664539783143	39.0	37.0	39.0	32.0	39.0
10	36.43341172959684	38.0	35.0	39.0	32.0	39.0
11	36.591727181003	38.0	35.0	39.0	32.0	39.0
12	36.514747260867715	38.0	35.0	39.0	32.0	39.0
13	36.4876401540451	38.0	35.0	39.0	32.0	39.0
14	37.390821952848555	39.0	36.0	40.0	32.0	41.0
15	37.51859128309341	39.0	36.0	40.0	32.0	41.0
16	37.34310918159985	39.0	36.0	40.0	32.0	41.0
17	37.36052807344143	39.0	36.0	40.0	32.0	41.0
18	37.19620500504484	39.0	36.0	40.0	32.0	41.0
19	37.0571629552786	39.0	35.0	40.0	31.0	41.0
20	37.13453367249783	39.0	36.0	40.0	31.0	41.0
21	37.04592576276485	39.0	35.0	40.0	31.0	41.0
22	37.042145689152896	39.0	35.0	40.0	31.0	41.0
23	36.870838011056	39.0	35.0	40.0	31.0	41.0
24	36.87641219855334	39.0	35.0	40.0	31.0	41.0
25	36.75337506572497	39.0	35.0	40.0	31.0	41.0
26	36.74320013073939	39.0	35.0	40.0	31.0	41.0
27	36.637873211215165	39.0	35.0	40.0	30.0	41.0
28	36.396154556694	39.0	35.0	40.0	30.0	41.0
29	36.18973553695519	38.0	35.0	40.0	30.0	41.0
30	36.34784493171709	39.0	35.0	40.0	30.0	41.0
31	36.04993676192642	38.0	35.0	40.0	29.0	41.0
32	36.209723031448505	38.0	35.0	40.0	30.0	41.0
33	36.07524264946212	38.0	35.0	40.0	29.0	41.0
34	36.01782034702781	38.0	35.0	40.0	29.0	41.0
35	36.05817192229533	38.0	35.0	40.0	29.0	41.0
36	35.87796472878682	38.0	35.0	40.0	29.0	41.0
37	35.860272278986486	38.0	35.0	40.0	28.0	41.0
38	35.66825235544061	38.0	35.0	40.0	27.0	41.0
39	35.552643919907915	38.0	34.0	40.0	27.0	41.0
40	35.33339254501272	38.0	34.0	40.0	26.0	41.0
41	35.46743949750601	38.0	34.0	40.0	27.0	41.0
42	35.51524463897455	38.0	35.0	40.0	27.0	41.0
43	35.40974363711293	38.0	34.0	40.0	26.0	41.0
44	35.44419417641291	38.0	34.0	40.0	27.0	41.0
45	35.11578251787009	38.0	34.0	40.0	26.0	41.0
46	34.898279071750345	38.0	33.0	40.0	25.0	41.0
47	34.85667339311345	38.0	33.0	40.0	24.0	41.0
48	34.85460927397007	38.0	33.0	40.0	24.0	41.0
49	34.715553013400786	38.0	33.0	40.0	24.0	41.0
50	34.52783185777828	38.0	33.0	40.0	23.0	41.0
51	33.05730151060836	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	2.0
15	9.0
16	31.0
17	63.0
18	114.0
19	286.0
20	586.0
21	965.0
22	1403.0
23	1859.0
24	2120.0
25	2408.0
26	2710.0
27	3270.0
28	4152.0
29	5341.0
30	7036.0
31	9091.0
32	11880.0
33	14982.0
34	20049.0
35	25060.0
36	28494.0
37	37374.0
38	52042.0
39	50140.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.013088149611335	41.72078614162486	17.950731145817052	24.31539456294675
2	16.30334380195825	40.189927382796405	19.178189259475054	24.328539555770295
3	17.440918586309312	28.723940939902516	28.06136224758061	25.77377822620756
4	23.950532194574315	29.67713055464764	17.70701587346701	28.66532137731103
5	13.997285736616977	40.34198297545794	17.89069050292032	27.770040785004763
6	19.52919609487132	32.625516917961036	25.226307038610752	22.618979948556895
7	67.7773593485768	12.729326834259405	10.994187781551537	8.499126035612273
8	68.40831900410691	5.969603092270744	8.397163523710725	17.22491437991161
9	64.47761087979082	9.373090423339823	17.53435461637937	8.61494408048999
10	23.262729326834258	39.180605095993975	16.511176796600775	21.045488780570988
11	17.866532137731102	27.48866688456565	35.013287100854065	19.63151387684918
12	20.30439540138413	19.335929173357584	42.8310051300999	17.52867029515838
13	27.231806619392064	23.931347610453464	28.368670863590502	20.46817490656397
14	14.386306470178631	29.025565234691413	36.75197885432506	19.836149440804903
15	12.093393397660902	28.148758686353364	34.98202333413861	24.775824581847118
16	13.511276272222142	29.970938907757677	31.414756497889694	25.103028322130484
17	13.483209936193493	26.852733447967143	31.71744660290753	27.94661001293183
18	17.911296167346418	24.538504170870695	29.04830251957538	28.501897142207504
19	17.64910685102815	31.705367420312925	33.429493100655115	17.21603262800381
20	17.640580369196663	30.551450212451503	27.876977077974672	23.930992340377156
21	16.064602310676577	29.76985604456508	28.89056260569285	25.2749790390655
22	13.741491281672328	31.351518424306157	28.404197871221704	26.502792422799814
23	18.438161690517134	26.561056715314983	24.315749833023066	30.68503176114482
24	11.600989071892451	33.45933578706533	25.179411388537567	29.760263752504656
25	15.460643180946153	27.0850800778752	26.96997257315011	30.484304168028537
26	20.188222086430105	27.482272023192028	27.160752604129662	25.168753286248208
27	13.37272094246046	25.44728502607682	27.77181713538632	33.4081768960764
28	19.86457104690986	23.733817448023988	28.12175816055365	28.2798533445125
29	17.585868777444613	28.971208913015673	27.260583495573336	26.18233881396638
30	18.276158535718853	23.635052366809248	23.348704685301765	34.74008441217013
31	17.697423581406586	34.339339766090184	21.696343560374597	26.266893092128633
32	16.640139834302037	21.953203825548183	21.068226065454958	40.33843027469482
33	16.798235018260883	26.312367661896573	27.64285409768506	29.246543222157484
34	13.336128124600322	25.968110957950234	25.212096235558274	35.48366468189118
35	15.069490826926629	21.623157924654322	25.81179212437295	37.4955591240461
36	14.1163012121815	26.531924569057395	34.97136523184925	24.38040898691185
37	15.947363185493613	27.11669911466697	21.372337250778042	35.563600449061376
38	18.404766303343802	27.8776876181273	28.29619576802285	25.421350310506046
39	16.79645866787932	24.290170387528597	28.327104264661994	30.586266679930084
40	18.805866219500064	27.032144836504713	25.720487714760758	28.44150122923446
41	15.247481135158946	22.394804530404013	31.832909377708933	30.5248049567281
42	22.79164120564453	21.807543094260257	24.03757336327076	31.363242336824452
43	16.475294518893264	21.25225596498458	32.690886612002444	29.581562904119714
44	20.013784478960904	19.027199477042448	25.96668987764499	34.99232616635166
45	23.989611902968637	23.466654350637352	29.194318520939618	23.34941522545439
46	15.53915786781111	21.02630419645014	36.49263169861729	26.94190623712146
47	19.618013613949326	23.2698347283605	26.74544188492092	30.36670977276926
48	18.023916781537324	20.506544074805667	35.59841691653995	25.871122227117056
49	21.21708422742969	16.000653696940415	30.288195085904306	32.49406698972559
50	18.6442183347781	16.825590814136905	28.388565987863974	36.141624863221026
51	17.95108641589336	14.791314357174324	37.94852847134392	29.309070755588394
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2082.0
1	1633.5
2	1185.0
3	832.0
4	479.0
5	420.5
6	362.0
7	392.5
8	423.0
9	446.0
10	469.0
11	537.5
12	606.0
13	629.5
14	653.0
15	674.5
16	696.0
17	695.5
18	695.0
19	666.0
20	637.0
21	663.0
22	689.0
23	815.0
24	941.0
25	942.5
26	1230.5
27	1517.0
28	1848.0
29	2179.0
30	2455.0
31	2731.0
32	3352.0
33	3973.0
34	4085.5
35	4198.0
36	4702.0
37	5206.0
38	5805.5
39	6405.0
40	7291.0
41	8177.0
42	11620.0
43	15063.0
44	27125.0
45	39187.0
46	39150.5
47	39114.0
48	40047.5
49	40981.0
50	36433.0
51	31885.0
52	26601.0
53	21317.0
54	17649.5
55	13982.0
56	11682.5
57	9383.0
58	8030.0
59	6677.0
60	6048.5
61	5420.0
62	5156.0
63	4892.0
64	4100.5
65	3309.0
66	2446.0
67	1583.0
68	1385.0
69	1187.0
70	1065.0
71	943.0
72	723.5
73	504.0
74	460.5
75	264.5
76	112.0
77	133.0
78	154.0
79	97.5
80	41.0
81	29.0
82	17.0
83	21.0
84	25.0
85	18.5
86	12.0
87	17.0
88	22.0
89	11.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	281476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.47461240034674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71163091814101	25.678921115832253
2	10.377508676225549	6.947661612357714
3	3.9724907931184528	3.989327686907587
4	2.013308852403341	2.6957893390555503
5	1.2183861689820956	2.0392502380309514
6	0.8702758349872112	1.7479287754551007
7	0.5423304288761768	1.2708010629680684
8	0.4372605414813793	1.1709701715243928
9	0.3152096621843924	0.9496369139820091
>10	2.9164853592010442	21.915189927382798
>50	0.5274720609617609	11.64113459051571
>100	0.07853708754762637	5.183390413392261
>500	0.0074291839572078995	1.806548338046583
>1k	0.0074291839572078995	3.693387713339681
>5k	0.004245247975547372	9.270062101209339
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	7174	2.548707527462377	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT	6545	2.3252426494621212	No Hit
AATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	6313	2.2428199917577345	No Hit
CTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGCT	6061	2.1532919325271074	TruSeq Adapter, Index 20 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1774	0.6302491153775099	No Hit
AATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	1701	0.6043143998067331	No Hit
AAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	1675	0.5950773778226208	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	1545	0.5488922679020591	No Hit
AATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	1382	0.4909832454632011	No Hit
AACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	1268	0.45048245676363174	TruSeq Adapter, Index 23 (95% over 21bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTAT	1051	0.37338885020392504	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACTC	927	0.3293353607412355	No Hit
ACCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTC	901	0.3200983387571232	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	847	0.30091375463627446	No Hit
CCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	801	0.2845713311259219	TruSeq Adapter, Index 23 (95% over 22bp)
AATGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	545	0.19362219159004676	No Hit
ACCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTAC	536	0.19042476090323862	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	528	0.18758260029274254	No Hit
ACCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	470	0.16697693586664583	No Hit
ACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTG	466	0.16555585556139776	TruSeq Adapter, Index 20 (95% over 21bp)
GAAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	435	0.1545424831957254	No Hit
AACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	410	0.1456607312879251	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	401	0.14246330060111698	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCC	358	0.12718668731970043	No Hit
TACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	334	0.11866020548821213	No Hit
AATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATG	313	0.11119953388565988	No Hit
AATGATCCCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGT	307	0.10906791342778781	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	299	0.10622575281729171	No Hit
GAAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	292	0.10373886228310762	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	289	0.10267305205417158	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.381375321519419	0.0
2	0.0	0.0	0.0	3.0240588895678493	0.0
3	0.0	0.0	0.0	4.119711804914096	0.0
4	0.0	0.0	0.0	9.560317753556253	0.0
5	0.0	0.0	0.0	10.294305731216872	0.0
6	0.0	0.0	0.0	13.887507283036564	0.0
7	0.0	0.0	0.0	16.677087922238485	0.0
8	0.0	0.0	0.0	19.143372791996477	0.0
9	0.0	0.0	0.0	24.06102118830735	0.0
10	0.0	0.0	0.0	28.448961900837016	0.0
11	0.0	0.0	0.0	33.63235231422928	0.0
12	3.552700763120124E-4	0.0	0.0	35.43534795151274	0.0
13	3.552700763120124E-4	0.0	0.0	36.451420369765096	0.0
14	3.552700763120124E-4	0.0	0.0	37.48845372251986	0.0
15	3.552700763120124E-4	0.0	0.0	38.160979976978496	0.0
16	3.552700763120124E-4	0.0	0.0	39.49892708436954	0.0
17	3.552700763120124E-4	0.0	0.0	40.918231039236026	0.0
18	3.552700763120124E-4	0.0	0.0	44.216203157640436	0.0
19	3.552700763120124E-4	0.0	0.0	44.93704614247751	0.0
20	3.552700763120124E-4	0.0	0.0	46.233426650940046	0.0
21	3.552700763120124E-4	0.0	0.0	46.989086103255694	0.0
22	3.552700763120124E-4	0.0	0.0	47.75789054839489	0.0
23	3.552700763120124E-4	0.0	0.0	48.47766772300303	0.0
24	3.552700763120124E-4	0.0	0.0	48.86242521564893	0.0
25	3.552700763120124E-4	0.0	0.0	49.21058989043471	0.0
26	3.552700763120124E-4	0.0	0.0	49.56905739743353	0.0
27	3.552700763120124E-4	0.0	0.0	50.24407054242635	0.0
28	3.552700763120124E-4	0.0	0.0	50.691000298426864	0.0
29	3.552700763120124E-4	0.0	0.0	51.10986371839873	0.0
30	3.552700763120124E-4	0.0	0.0	51.92734016399267	0.0
31	3.552700763120124E-4	0.0	0.0	52.292557802441415	0.0
32	3.552700763120124E-4	0.0	0.0	52.67980218562151	0.0
33	3.552700763120124E-4	0.0	0.0	53.04004604300189	0.0
34	3.552700763120124E-4	0.0	0.0	53.37435518481149	0.0
35	3.552700763120124E-4	0.0	0.0	53.72678310051301	0.0
36	3.552700763120124E-4	0.0	0.0	54.036223336980775	0.0
37	3.552700763120124E-4	0.0	0.0	54.35596640566158	0.0
38	3.552700763120124E-4	0.0	0.0	54.669314612968776	0.0
39	3.552700763120124E-4	0.0	0.0	55.023518879051856	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGAAC	25	3.8832753E-5	45.000004	32
TAATAGG	50	2.1827873E-11	45.000004	2
AATCCGG	25	3.8832753E-5	45.000004	2
TACCCGG	25	3.8832753E-5	45.000004	2
ACAAAGT	25	3.8832753E-5	45.000004	12
CGAACAG	25	3.8832753E-5	45.000004	1
CCGGCGA	35	1.2075543E-7	45.000004	7
CTTCACT	25	3.8832753E-5	45.000004	13
GGCAAAC	25	3.8832753E-5	45.000004	8
ATATGGG	95	0.0	45.000004	3
TACGGGC	70	0.0	45.000004	4
AATATGG	35	1.2075543E-7	45.000004	2
CACCTGG	35	1.2075543E-7	45.000004	2
TAGTTAT	25	3.8832753E-5	45.000004	27
GTTACTA	25	3.8832753E-5	45.000004	44
CTGCGCG	35	1.2075543E-7	45.000004	1
ATTCAGT	25	3.8832753E-5	45.000004	28
AACATAG	25	3.8832753E-5	45.000004	1
GGGCGTA	25	3.8832753E-5	45.000004	7
TGCGAAG	35	1.2075543E-7	45.000004	1
>>END_MODULE