FastQCFastQC Report
Sat 18 Jun 2016
SRR3548475_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548475_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529889
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT39020.7363806382091344No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10970.2070244900347054No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG9190.17343254908103395No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT8750.16512892322731743No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC7940.1498427029057029No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC7420.14002932689676517No Hit
AAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT6670.12587541919156653No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGT6520.1230446376505268No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT6030.11379741794979703No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA5630.1062486671736911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACCG302.1632786E-645.0000041
ACTAGCG302.1632786E-645.0000041
CGTGGAC207.029842E-445.021
TAATACG207.029842E-445.01
TTAATCG551.8189894E-1245.020
GAATGCG207.029842E-445.01
GAACTCG207.029842E-445.036
CGCATAG351.2102646E-745.01
GGGTACG406.8030204E-945.07
TTATCCG207.029842E-445.01
CCATGCG253.8879494E-545.01
TATTGTC207.029842E-445.019
CGTAGCG253.8879494E-545.01
AACGATT207.029842E-445.012
CGAATAG207.029842E-445.01
ACTCGTC207.029842E-445.015
CGTAATG253.8879494E-545.01
GATTGCG207.029842E-445.01
CACGTTG207.029842E-445.017
CAACTCG207.029842E-445.01