##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548475_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	529889
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.849762874866247	33.0	31.0	34.0	30.0	34.0
2	32.04621345225132	34.0	31.0	34.0	30.0	34.0
3	32.18025095821955	34.0	31.0	34.0	30.0	34.0
4	35.78072200026798	37.0	35.0	37.0	35.0	37.0
5	35.61324164117391	37.0	35.0	37.0	33.0	37.0
6	35.614300353470256	37.0	35.0	37.0	33.0	37.0
7	35.675900046990975	37.0	35.0	37.0	35.0	37.0
8	35.834801250828	37.0	35.0	37.0	35.0	37.0
9	37.400159278641375	39.0	37.0	39.0	35.0	39.0
10	37.014748371828816	39.0	37.0	39.0	33.0	39.0
11	37.0118137949646	39.0	37.0	39.0	33.0	39.0
12	36.94505075591303	39.0	37.0	39.0	33.0	39.0
13	36.87900673537288	39.0	37.0	39.0	33.0	39.0
14	38.08247198941665	40.0	37.0	41.0	33.0	41.0
15	38.070197720654704	40.0	37.0	41.0	33.0	41.0
16	38.0272943956187	40.0	37.0	41.0	33.0	41.0
17	38.010385193880225	40.0	37.0	41.0	33.0	41.0
18	37.98062046957004	40.0	37.0	41.0	33.0	41.0
19	37.924327547844925	40.0	37.0	41.0	33.0	41.0
20	37.85700401404822	40.0	37.0	41.0	33.0	41.0
21	37.80846365937017	40.0	37.0	41.0	33.0	41.0
22	37.78718750530771	40.0	37.0	41.0	33.0	41.0
23	37.6650543793134	39.0	36.0	41.0	33.0	41.0
24	37.59387532105781	39.0	36.0	41.0	32.0	41.0
25	37.549260316783325	39.0	36.0	41.0	32.0	41.0
26	37.47596194674734	39.0	36.0	41.0	32.0	41.0
27	37.41279400025288	39.0	36.0	41.0	32.0	41.0
28	37.29961935424212	39.0	36.0	41.0	32.0	41.0
29	37.149027437821886	39.0	36.0	41.0	31.0	41.0
30	37.14575882873583	39.0	36.0	41.0	31.0	41.0
31	37.069022002721326	39.0	36.0	41.0	31.0	41.0
32	37.01257244441761	39.0	35.0	41.0	31.0	41.0
33	36.99518767138023	39.0	35.0	41.0	31.0	41.0
34	36.88348880614619	39.0	35.0	41.0	31.0	41.0
35	36.78876141984453	39.0	35.0	41.0	31.0	41.0
36	36.73943976946115	39.0	35.0	40.0	31.0	41.0
37	36.62730685105748	39.0	35.0	40.0	30.0	41.0
38	36.52238110245731	39.0	35.0	40.0	30.0	41.0
39	36.43702926461957	39.0	35.0	40.0	30.0	41.0
40	36.332212972905644	39.0	35.0	40.0	30.0	41.0
41	36.262987153913365	38.0	35.0	40.0	30.0	41.0
42	36.150982564272894	38.0	35.0	40.0	30.0	41.0
43	36.08730318991336	38.0	35.0	40.0	30.0	41.0
44	36.00340259941233	38.0	35.0	40.0	30.0	41.0
45	35.808554244379486	38.0	35.0	40.0	29.0	41.0
46	35.6419608635016	38.0	34.0	40.0	29.0	41.0
47	35.52614604190689	38.0	34.0	40.0	28.0	41.0
48	35.47614311676596	38.0	34.0	40.0	28.0	41.0
49	35.28797540616997	37.0	34.0	40.0	27.0	41.0
50	35.11957221229352	37.0	34.0	40.0	27.0	41.0
51	33.859374321791925	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	1.0
11	9.0
12	10.0
13	9.0
14	14.0
15	28.0
16	52.0
17	106.0
18	189.0
19	371.0
20	604.0
21	924.0
22	1420.0
23	1860.0
24	2399.0
25	2993.0
26	3749.0
27	4664.0
28	5807.0
29	7528.0
30	9766.0
31	13040.0
32	16673.0
33	22266.0
34	33119.0
35	42560.0
36	51831.0
37	73399.0
38	108219.0
39	126239.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.832476235588963	30.443734442496446	20.214988422103495	28.508800899811092
2	26.518195320151953	29.340673235337967	18.628807165274235	25.512324279235838
3	21.420901358586423	29.83964566163857	20.050048217645582	28.689404762129428
4	21.990265885874212	31.796093144035826	18.978691763746745	27.234949206343217
5	19.503896098994318	32.825176593588466	16.99940931025177	30.67151799716544
6	20.531847235930545	36.73712796453597	19.608635016012787	23.1223897835207
7	83.01587691006984	5.25223207124511	6.268671363247774	5.463219655437271
8	84.5724293201029	4.626818069444733	5.097293961565535	5.703458648886842
9	76.2652178097677	8.291736571244167	9.68504724574392	5.757998373244208
10	33.64214014633254	34.1605506058816	13.892532209575966	18.304777038209888
11	25.85711347093448	29.32104648332009	25.518740717395534	19.303099328349898
12	26.35325511569404	23.749124816706896	29.729622619076824	20.167997448522236
13	27.197960327540294	24.429078542864637	26.81844688227157	21.554514247323496
14	21.310500878485872	27.62314371500446	28.506158837039457	22.560196569470207
15	20.085904783832085	28.635619912849673	28.885861001077583	22.392614302240656
16	21.877978218079637	26.64614664580695	27.46707329270847	24.00880184340494
17	20.41257697366807	27.15417757303888	28.55767906108638	23.87556639220667
18	23.712135937903973	25.032223729875504	25.861265283861336	25.394375048359187
19	22.633230733229034	29.798693688678195	26.134718780725773	21.433356797366994
20	24.565522213142753	27.137192883792643	25.308696727050382	22.988588176014222
21	23.622305803668315	28.199868274298957	25.631594541498316	22.546231380534415
22	21.99253051110704	25.801818871499506	24.871057900805642	27.334592716587814
23	21.92327072273627	28.87680250014626	23.871792016818617	25.328134760298855
24	20.535621611318597	27.432915195446594	26.225115071269645	25.80634812196517
25	23.18108132080492	27.634655559938025	23.55436704668336	25.629896072573686
26	25.00731285231435	27.401021723417546	24.278103527342516	23.313561896925584
27	21.274078155991162	26.039981958485647	25.074496734221697	27.6114431513015
28	22.92857560734418	28.157217832413963	26.470449471493083	22.443757088748775
29	24.225073553140376	29.079109020945896	23.334320961559875	23.361496464353856
30	23.152018630316913	26.71653874679414	24.775754922257303	25.35568770063164
31	24.946922846105505	26.807501193646218	22.81723153339662	25.428344426851663
32	23.04822330714546	28.739415236021127	23.18730904019521	25.0250524166382
33	22.551704224847093	25.42249414500018	24.514190707865232	27.5116109222875
34	20.381627095486035	28.321969318102468	25.172064338002865	26.124339248408628
35	22.899701635625576	26.88770667064234	25.37795651542115	24.83463517831093
36	23.097101468420746	27.84583186289959	24.4407791065676	24.616287562112067
37	21.16915052020329	28.95455463314015	23.230336919619017	26.64595792703755
38	21.95044622553025	28.612407504213145	25.14224677243725	24.294899497819355
39	21.56413890456303	24.92597506270181	24.225450990679178	29.28443504205598
40	23.36847905882175	25.51156940415823	24.80123195612666	26.318719580893358
41	20.529016454389502	25.1171471761067	27.57030245957172	26.78353390993208
42	24.23469821037991	27.398190941876504	23.948034399657285	24.419076448086297
43	22.51905587774043	24.581940746080782	26.66539596028602	26.233607415892763
44	23.636082273834706	24.136187012751726	25.196597778025208	27.031132935388353
45	24.427002636401205	23.392823780074696	27.639562247942496	24.540611335581602
46	20.79039195001217	24.76197845209091	27.865836052456267	26.581793545440647
47	21.982905853867507	26.942435113769108	24.597226966402395	26.477432065960983
48	22.3101442000117	24.501735269084655	27.64409149840816	25.544029032495487
49	22.676447331422242	24.595528497477773	26.94809667685119	25.779927494248795
50	21.667179352656877	23.610227802426547	26.721634153568015	28.000958691348565
51	22.24050697410212	22.315617044324377	28.76866664527854	26.67520933629496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1401.0
1	1158.0
2	915.0
3	656.5
4	398.0
5	392.0
6	386.0
7	433.0
8	480.0
9	532.5
10	585.0
11	673.5
12	762.0
13	838.0
14	914.0
15	873.5
16	833.0
17	937.5
18	1042.0
19	1192.5
20	1343.0
21	1419.5
22	1496.0
23	1801.5
24	2107.0
25	2103.5
26	2567.0
27	3034.0
28	4134.0
29	5234.0
30	5479.0
31	5724.0
32	7433.0
33	9142.0
34	10639.0
35	12136.0
36	12150.0
37	12164.0
38	13438.0
39	14712.0
40	16618.0
41	18524.0
42	21870.5
43	25217.0
44	28814.0
45	32411.0
46	38571.0
47	44731.0
48	50794.5
49	56858.0
50	55310.0
51	53762.0
52	48237.5
53	42713.0
54	38520.0
55	34327.0
56	30482.0
57	26637.0
58	25153.0
59	23669.0
60	22599.0
61	21529.0
62	20115.5
63	18702.0
64	16495.0
65	14288.0
66	12425.5
67	10563.0
68	9875.5
69	9188.0
70	7970.0
71	6752.0
72	5483.0
73	4214.0
74	3888.5
75	3012.0
76	2461.0
77	1941.0
78	1421.0
79	1085.0
80	749.0
81	575.0
82	401.0
83	303.0
84	205.0
85	124.5
86	44.0
87	33.0
88	22.0
89	17.5
90	13.0
91	10.0
92	7.0
93	5.5
94	4.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	529889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.636986068964205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80474921282968	22.91792059525824
2	10.08293767950659	6.178216424425553
3	3.7314361925640043	3.429598759464367
4	1.8390133941616131	2.2536731095027167
5	1.1339424446777475	1.7370289440299682
6	0.8020363677857061	1.4743186215992
7	0.5816361299256925	1.2473704606817786
8	0.4690834832687092	1.1497043313667716
9	0.40162632980856916	1.107415824414692
>10	5.3852383971924995	39.56495097834908
>50	0.6928919652783315	13.370497285892114
>100	0.06922738636143902	3.5214821336494033
>500	0.004944813311531358	1.1011732957179652
>1k	0.0012362033278828395	0.9466492356481698
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	3902	0.7363806382091344	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1097	0.2070244900347054	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	919	0.17343254908103395	No Hit
AATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	875	0.16512892322731743	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCC	794	0.1498427029057029	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	742	0.14002932689676517	No Hit
AAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	667	0.12587541919156653	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGT	652	0.1230446376505268	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTT	603	0.11379741794979703	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	563	0.1062486671736911	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8130004585866096	0.0
2	0.0	0.0	0.0	1.1200458964047186	0.0
3	0.0	0.0	0.0	1.4446421797772742	0.0
4	0.0	0.0	0.0	2.8896617970933534	0.0
5	0.0	0.0	0.0	3.1344300410085886	0.0
6	0.0	0.0	0.0	4.666449011019289	0.0
7	0.0	0.0	0.0	5.763659936326287	0.0
8	0.0	0.0	0.0	6.9433409638622425	0.0
9	0.0	0.0	0.0	8.264561068450185	0.0
10	0.0	0.0	0.0	10.265546180426467	0.0
11	0.0	0.0	0.0	11.90607844284369	0.0
12	0.0	0.0	0.0	12.789471002417487	0.0
13	0.0	0.0	0.0	13.181817324005594	0.0
14	0.0	0.0	0.0	13.612473555782438	0.0
15	0.0	0.0	0.0	13.952356059476607	0.0
16	0.0	0.0	0.0	14.662504788738774	0.0
17	0.0	0.0	0.0	15.500038687347727	0.0
18	0.0	0.0	0.0	16.614423020670365	0.0
19	0.0	0.0	0.0	17.041116158289753	0.0
20	0.0	0.0	0.0	17.567830243692548	0.0
21	0.0	0.0	0.0	18.090015078629676	0.0
22	0.0	0.0	0.0	18.660889356072687	0.0
23	0.0	0.0	0.0	19.26460069939176	0.0
24	0.0	0.0	0.0	19.691671274549954	0.0
25	0.0	0.0	0.0	20.06288109396496	0.0
26	0.0	0.0	0.0	20.406537973047186	0.0
27	0.0	0.0	0.0	20.837005486054625	0.0
28	0.0	0.0	0.0	21.232182589183772	0.0
29	0.0	0.0	0.0	21.644344381559158	0.0
30	0.0	0.0	0.0	22.248244443647632	0.0
31	0.0	0.0	0.0	22.63511792092306	0.0
32	0.0	0.0	0.0	23.03388068067086	0.0
33	0.0	0.0	0.0	23.449250692126085	0.0
34	0.0	0.0	0.0	23.78800088320384	0.0
35	0.0	0.0	0.0	24.201483706965043	0.0
36	0.0	0.0	0.0	24.569296588530804	0.0
37	0.0	0.0	0.0	24.928994563012253	0.0
38	1.887187694026485E-4	0.0	0.0	25.30718697689516	0.0
39	1.887187694026485E-4	0.0	0.0	25.686322984625082	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACCG	30	2.1632786E-6	45.000004	1
ACTAGCG	30	2.1632786E-6	45.000004	1
CGTGGAC	20	7.029842E-4	45.0	21
TAATACG	20	7.029842E-4	45.0	1
TTAATCG	55	1.8189894E-12	45.0	20
GAATGCG	20	7.029842E-4	45.0	1
GAACTCG	20	7.029842E-4	45.0	36
CGCATAG	35	1.2102646E-7	45.0	1
GGGTACG	40	6.8030204E-9	45.0	7
TTATCCG	20	7.029842E-4	45.0	1
CCATGCG	25	3.8879494E-5	45.0	1
TATTGTC	20	7.029842E-4	45.0	19
CGTAGCG	25	3.8879494E-5	45.0	1
AACGATT	20	7.029842E-4	45.0	12
CGAATAG	20	7.029842E-4	45.0	1
ACTCGTC	20	7.029842E-4	45.0	15
CGTAATG	25	3.8879494E-5	45.0	1
GATTGCG	20	7.029842E-4	45.0	1
CACGTTG	20	7.029842E-4	45.0	17
CAACTCG	20	7.029842E-4	45.0	1
>>END_MODULE