FastQCFastQC Report
Sat 18 Jun 2016
SRR3548474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592430
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT32160.542848944179059No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12150.2050875208885438No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG10850.18314400013503704No Hit
AAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT9470.1598501088736222No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT7130.12035177151731005No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG302.1637134E-645.0000041
TAACGAG650.045.0000041
ACGATAG302.1637134E-645.0000041
CTAACCG453.8380676E-1045.0000041
GTTAACG302.1637134E-645.0000041
CGCTACG453.8380676E-1045.0000041
GTACTAG453.8380676E-1045.0000041
CGTGTTA302.1637134E-645.00000441
TATAATC302.1637134E-645.00000437
CGATACG302.1637134E-645.0000041
TGCCGGT302.1637134E-645.00000426
AAACTCG207.030515E-445.018
CGGCGTA207.030515E-445.042
AGTCCGT253.8885097E-545.016
TCGTTAC253.8885097E-545.010
GGTAATC253.8885097E-545.035
GATCGCG207.030515E-445.029
CGAAATA207.030515E-445.013
AGATACG253.8885097E-545.01
GCGCGTT207.030515E-445.09