##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548473_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	599416
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8498254968169	33.0	31.0	34.0	30.0	34.0
2	32.04781320485272	33.0	31.0	34.0	30.0	34.0
3	32.194614424706714	34.0	31.0	34.0	30.0	34.0
4	35.85757470604722	37.0	35.0	37.0	35.0	37.0
5	35.64985919628438	37.0	35.0	37.0	33.0	37.0
6	35.673895925367354	37.0	35.0	37.0	33.0	37.0
7	35.783557662791786	37.0	35.0	37.0	35.0	37.0
8	35.82792417953475	37.0	35.0	37.0	35.0	37.0
9	37.562502502435706	39.0	37.0	39.0	35.0	39.0
10	37.026137106783935	39.0	37.0	39.0	33.0	39.0
11	37.013544850320976	39.0	37.0	39.0	33.0	39.0
12	36.92573104488368	39.0	37.0	39.0	33.0	39.0
13	36.86397093170686	39.0	37.0	39.0	33.0	39.0
14	38.06303969196685	40.0	37.0	41.0	33.0	41.0
15	38.06987467801994	40.0	37.0	41.0	33.0	41.0
16	38.00365856099937	40.0	37.0	41.0	33.0	41.0
17	37.96640396652742	40.0	37.0	41.0	33.0	41.0
18	37.9581592750277	40.0	37.0	41.0	33.0	41.0
19	37.96243510350074	40.0	37.0	41.0	33.0	41.0
20	37.85896105542728	40.0	37.0	41.0	33.0	41.0
21	37.818900396385814	40.0	37.0	41.0	33.0	41.0
22	37.795474261614636	40.0	37.0	41.0	33.0	41.0
23	37.64089046672094	40.0	36.0	41.0	33.0	41.0
24	37.49666008248028	39.0	36.0	41.0	32.0	41.0
25	37.56262762422091	39.0	36.0	41.0	32.0	41.0
26	37.50091922804864	39.0	36.0	41.0	32.0	41.0
27	37.4326844795601	39.0	36.0	41.0	32.0	41.0
28	37.35853230477665	39.0	36.0	41.0	32.0	41.0
29	37.19007333804903	39.0	36.0	41.0	32.0	41.0
30	37.24001361325023	39.0	36.0	41.0	32.0	41.0
31	37.17363066718273	39.0	35.0	41.0	32.0	41.0
32	37.123616987200876	39.0	35.0	41.0	32.0	41.0
33	37.10302861451813	39.0	35.0	41.0	31.0	41.0
34	36.96196631387884	39.0	35.0	41.0	31.0	41.0
35	36.901851135104835	39.0	35.0	41.0	31.0	41.0
36	36.79464678954182	39.0	35.0	41.0	31.0	41.0
37	36.656223724425104	39.0	35.0	41.0	31.0	41.0
38	36.560176905521374	39.0	35.0	40.0	30.0	41.0
39	36.52075687002015	39.0	35.0	40.0	30.0	41.0
40	36.37743737237578	39.0	35.0	40.0	30.0	41.0
41	36.28816548106824	38.0	35.0	40.0	30.0	41.0
42	36.232282755215074	38.0	35.0	40.0	30.0	41.0
43	36.140590174436454	38.0	35.0	40.0	30.0	41.0
44	36.0450405060926	38.0	35.0	40.0	30.0	41.0
45	35.884998064783055	38.0	35.0	40.0	30.0	41.0
46	35.72876266232466	38.0	35.0	40.0	29.0	41.0
47	35.555684199287306	38.0	34.0	40.0	29.0	41.0
48	35.47973027079691	37.0	34.0	40.0	29.0	41.0
49	35.28024110133864	37.0	34.0	40.0	28.0	41.0
50	35.22669398214262	37.0	34.0	40.0	28.0	41.0
51	33.9755178373617	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	8.0
12	8.0
13	16.0
14	20.0
15	40.0
16	65.0
17	155.0
18	291.0
19	527.0
20	708.0
21	1199.0
22	1704.0
23	2151.0
24	2678.0
25	3349.0
26	4208.0
27	4951.0
28	6306.0
29	8037.0
30	10345.0
31	13750.0
32	17799.0
33	24240.0
34	38627.0
35	47785.0
36	57551.0
37	84558.0
38	125527.0
39	142771.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.830942116993874	29.77131074245599	21.23483523963324	28.162911900916892
2	25.28127377313919	30.417940128391635	19.21353450692007	25.08725159154911
3	21.641397627023636	30.848192240447368	19.473287333004123	28.037122799524873
4	19.995962737064076	33.20748862225899	18.76025998638675	28.036288654290175
5	18.874037396399164	33.88464772378448	16.94282434903306	30.298490530783294
6	20.407863653956518	37.05173035087485	18.762428763996958	23.777977231171672
7	86.30767280152682	4.529241795347472	5.120984424840177	4.042100978285531
8	87.69735876252886	4.045270730177373	3.94183672107518	4.315533786218586
9	81.71203304549762	6.710197925981289	7.227534800539191	4.350234227981902
10	38.0622138881845	31.460788500807453	12.746406502328933	17.730591108679114
11	27.826918200381705	30.410432821279375	21.31875025024357	20.44389872809535
12	27.342112989976915	24.448629999866537	27.975062394063556	20.234194616092996
13	25.36935950992299	25.407396532625086	26.66962510176572	22.5536188556862
14	22.520419875345336	26.111248281660814	27.966720941716606	23.40161090127724
15	20.74519198686722	29.865735983023477	26.28591829380597	23.103153736303337
16	22.461696050822802	27.121398160876588	26.724511858208654	23.692393930091956
17	20.899842513379692	26.98609980380904	27.49209230317509	24.62196537963618
18	25.04954822694089	24.324342359896967	25.856500326984932	24.769609086177212
19	23.079464011637995	29.53057642772298	24.398247627690953	22.99171193294807
20	26.005979153042297	26.92687549214569	24.330181376539834	22.736963978272186
21	24.637647310048447	27.640736983997762	25.351341972853575	22.37027373310022
22	24.513192841031938	24.596774193548388	24.337688683652086	26.55234428176759
23	23.187068746913663	28.948176224858862	22.93382225366023	24.930932774567246
24	21.0478198780146	28.275354678553793	26.7336874557903	23.943137987641304
25	23.83002789381665	27.411013386362725	23.46033472579978	25.298623994020847
26	25.907049528207455	26.013820118248425	23.59346430525712	24.485666048287
27	23.100984958693125	25.864841779331883	25.36919268087605	25.664980581098934
28	22.611008047833224	27.007620750864174	26.805423945974084	23.57594725532852
29	23.444652795387512	28.0633149598943	24.350868178360273	24.141164066357923
30	24.937939594538687	25.368859022782175	25.333157606737224	24.360043775941918
31	24.392408611048086	27.493760593644478	22.0803248495202	26.033505945787233
32	23.293505678860758	26.25071736490184	24.660336060432154	25.79544089580525
33	22.03945173302014	25.027526792744936	24.67668530703218	28.256336167202743
34	20.970411200234896	27.442043589093384	24.910412801793747	26.677132408877974
35	22.673735769482295	25.317475676324957	27.024804142698894	24.983984411493854
36	24.476156792611476	26.725012345349477	25.820131594752226	22.978699267286824
37	23.434976710665048	27.438206521013786	23.019739212833827	26.10707755548734
38	23.917779972506573	26.57736863880844	24.876046018124306	24.62880537056068
39	21.736657012825816	23.521560986026397	26.189991591816035	28.55179040933175
40	23.094812283956383	24.750423745779226	26.990103700935574	25.164660269328813
41	21.57983770870314	24.911580605122317	27.826918200381705	25.68166348579284
42	23.548754120677458	25.605422611341705	24.82065877454055	26.025164493440283
43	22.603834398814847	23.500206868018203	27.5314639582527	26.36449477491425
44	23.71191292858382	22.462863854151376	25.957932387523858	27.86729082974095
45	25.292951806424917	21.840257850974947	26.988769068560064	25.878021274040066
46	23.11049421436865	23.036922604668543	28.568806972119532	25.28377620884327
47	22.082326798083468	25.490310568953785	27.07168310488876	25.355679528073992
48	21.902651914530143	25.426415043976135	28.299878548453826	24.371054493039892
49	22.6190158420863	25.122285691406304	27.366970517970824	24.891727948536577
50	22.423325370026824	23.803668904400283	27.07051530156018	26.702490424012705
51	23.169885355078943	22.56946761514541	28.779345229356572	25.481301800419075
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1582.0
1	1254.5
2	927.0
3	654.0
4	381.0
5	416.0
6	451.0
7	477.0
8	503.0
9	519.0
10	535.0
11	575.0
12	615.0
13	754.0
14	893.0
15	983.5
16	1074.0
17	1061.5
18	1049.0
19	1246.5
20	1444.0
21	1787.0
22	2130.0
23	2505.5
24	2881.0
25	2860.0
26	3547.5
27	4256.0
28	5338.0
29	6420.0
30	6890.0
31	7360.0
32	9211.0
33	11062.0
34	12061.0
35	13060.0
36	13880.5
37	14701.0
38	16238.5
39	17776.0
40	18129.5
41	18483.0
42	21675.0
43	24867.0
44	29083.5
45	33300.0
46	39533.5
47	45767.0
48	49788.0
49	53809.0
50	54080.0
51	54351.0
52	51133.0
53	47915.0
54	44528.5
55	41142.0
56	37720.5
57	34299.0
58	33135.0
59	31971.0
60	29926.0
61	27881.0
62	25100.0
63	22319.0
64	20284.0
65	18249.0
66	16254.5
67	14260.0
68	12913.0
69	11566.0
70	10207.5
71	8849.0
72	7326.0
73	5803.0
74	5051.5
75	3808.5
76	3317.0
77	2635.0
78	1953.0
79	1705.5
80	1458.0
81	1016.0
82	574.0
83	661.5
84	749.0
85	422.0
86	95.0
87	101.5
88	108.0
89	73.0
90	38.0
91	33.0
92	28.0
93	22.0
94	16.0
95	12.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	599416.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.124354167014836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41526127220102	17.439292112563344
2	10.524985420418416	4.86766980928846
3	3.802461752833598	2.637884168371565
4	1.81941966358386	1.6829161871657665
5	1.0541110146103436	1.2187818216600468
6	0.676994504956849	0.9393036421047034
7	0.49102877055873567	0.7948306237615851
8	0.34562383093079346	0.63938623000033
9	0.27951245569684613	0.5817190517663323
>10	3.2551977547366135	19.043513490271394
>50	1.652278054510534	27.928504084187804
>100	0.67804380620072	20.73750225720127
>500	0.002903827863814647	0.49321046240747124
>1k	0.002177870897860985	0.9954860592499335
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	3572	0.5959133556661818	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1290	0.21520947055133663	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1068	0.17817342213087406	No Hit
AAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	988	0.1648270983757524	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	786	0.13112763089407023	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	654	0.1091061966981195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6682904693902063E-4	0.0	0.0	0.6716537429764972	0.0
2	1.6682904693902063E-4	0.0	0.0	0.921229997197272	0.0
3	1.6682904693902063E-4	0.0	0.0	1.22135545264057	0.0
4	1.6682904693902063E-4	0.0	0.0	2.495762542207749	0.0
5	1.6682904693902063E-4	0.0	0.0	2.6791076647937326	0.0
6	1.6682904693902063E-4	0.0	0.0	3.650386376072711	0.0
7	1.6682904693902063E-4	0.0	0.0	4.346397159902305	0.0
8	1.6682904693902063E-4	0.0	0.0	4.859229650192854	0.0
9	1.6682904693902063E-4	0.0	0.0	5.615298890920496	0.0
10	1.6682904693902063E-4	0.0	0.0	6.789942210418141	0.0
11	1.6682904693902063E-4	0.0	0.0	7.797255995835947	0.0
12	1.6682904693902063E-4	0.0	0.0	8.289568513352997	0.0
13	1.6682904693902063E-4	0.0	0.0	8.488095079210431	0.0
14	1.6682904693902063E-4	0.0	0.0	8.732666462023035	0.0
15	1.6682904693902063E-4	0.0	0.0	8.957552017296836	0.0
16	1.6682904693902063E-4	0.0	0.0	9.456204038597567	0.0
17	1.6682904693902063E-4	0.0	0.0	10.03176425053719	0.0
18	1.6682904693902063E-4	0.0	0.0	10.819364181136306	0.0
19	1.6682904693902063E-4	0.0	0.0	11.13717351555514	1.6682904693902063E-4
20	1.6682904693902063E-4	0.0	0.0	11.46616039611889	1.6682904693902063E-4
21	1.6682904693902063E-4	0.0	0.0	11.852035981688843	1.6682904693902063E-4
22	1.6682904693902063E-4	0.0	0.0	12.273779812350687	1.6682904693902063E-4
23	1.6682904693902063E-4	0.0	0.0	12.744070895671788	1.6682904693902063E-4
24	1.6682904693902063E-4	0.0	0.0	13.084068493333511	1.6682904693902063E-4
25	1.6682904693902063E-4	0.0	0.0	13.380523709744152	1.6682904693902063E-4
26	1.6682904693902063E-4	0.0	0.0	13.630433622058804	1.6682904693902063E-4
27	1.6682904693902063E-4	0.0	0.0	13.906702523789821	1.6682904693902063E-4
28	1.6682904693902063E-4	0.0	0.0	14.209830902078023	1.6682904693902063E-4
29	3.3365809387804127E-4	0.0	0.0	14.527306578402978	1.6682904693902063E-4
30	3.3365809387804127E-4	0.0	0.0	14.92569434249336	1.6682904693902063E-4
31	3.3365809387804127E-4	0.0	0.0	15.223651020326452	1.6682904693902063E-4
32	3.3365809387804127E-4	0.0	0.0	15.51510136532892	1.6682904693902063E-4
33	3.3365809387804127E-4	0.0	0.0	15.828906802621217	1.6682904693902063E-4
34	3.3365809387804127E-4	0.0	0.0	16.12836494187676	1.6682904693902063E-4
35	3.3365809387804127E-4	0.0	0.0	16.50189517797323	1.6682904693902063E-4
36	3.3365809387804127E-4	0.0	0.0	16.793345522975695	1.6682904693902063E-4
37	3.3365809387804127E-4	0.0	0.0	17.0926368331843	1.6682904693902063E-4
38	3.3365809387804127E-4	0.0	0.0	17.390259852923514	1.6682904693902063E-4
39	3.3365809387804127E-4	0.0	0.0	17.71607698159542	1.6682904693902063E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAG	65	0.0	45.000004	1
TATCATC	20	7.030583E-4	45.0	43
CTGTCGT	25	3.8885642E-5	45.0	19
AAATCGA	25	3.8885642E-5	45.0	40
GTCGAGT	20	7.030583E-4	45.0	38
CCGATAT	20	7.030583E-4	45.0	7
CGTGCAT	20	7.030583E-4	45.0	13
CGTTGTA	20	7.030583E-4	45.0	24
CTATACG	25	3.8885642E-5	45.0	1
ATTACGC	20	7.030583E-4	45.0	28
GGTCCGA	25	3.8885642E-5	45.0	11
GCGCCCA	25	3.8885642E-5	45.0	15
CGTCTAT	20	7.030583E-4	45.0	45
ATTCGAT	20	7.030583E-4	45.0	27
CGATTTA	20	7.030583E-4	45.0	29
ATAGACC	20	7.030583E-4	45.0	27
ACTTATG	20	7.030583E-4	45.0	1
TAACGAG	25	3.8885642E-5	45.0	1
CGTTCGA	25	3.8885642E-5	45.0	43
TAGCCCG	40	6.8048394E-9	45.0	28
>>END_MODULE