FastQCFastQC Report
Sat 18 Jun 2016
SRR3548471_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548471_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399452
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT22260.557263450927771No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22050.5520062485605279No Hit
AAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT10680.2673662918197931No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTT9910.24808988313990166No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC6270.15696504210768752No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC5470.13693760451818993No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4930.12341908414527902No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT4880.12216736929593544No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG4210.10539439031473118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG207.027754E-445.018
TATTTCG207.027754E-445.01
AACGTTC207.027754E-445.026
TAGAGTT253.88622E-545.037
AACGTAC351.2092642E-745.026
AAACTAT207.027754E-445.024
AACCGAT207.027754E-445.036
CGAACTT207.027754E-445.029
GCGACAC207.027754E-445.021
AGTTTCG207.027754E-445.020
CACGGTT207.027754E-445.022
CCGATAA207.027754E-445.038
ACGCACG253.88622E-545.01
CGCATTC253.88622E-545.025
CGCATAG406.7957444E-945.01
CCGGATT253.88622E-545.019
ACCGATA207.027754E-445.037
GTCTCGA207.027754E-445.031
TAAACGT207.027754E-445.024
GCCGGAT207.027754E-445.018