##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548471_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399452
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.748600582798435	31.0	31.0	34.0	30.0	34.0
2	31.942140732803942	33.0	31.0	34.0	30.0	34.0
3	32.10391736679251	34.0	31.0	34.0	30.0	34.0
4	35.7795604978821	37.0	35.0	37.0	35.0	37.0
5	35.544190541041225	37.0	35.0	37.0	33.0	37.0
6	35.56790052371749	37.0	35.0	37.0	33.0	37.0
7	35.632882048406316	37.0	35.0	37.0	35.0	37.0
8	35.6811531798564	37.0	35.0	37.0	35.0	37.0
9	37.40340516507615	39.0	37.0	39.0	35.0	39.0
10	36.94645414217478	39.0	37.0	39.0	33.0	39.0
11	36.89581476622973	39.0	37.0	39.0	33.0	39.0
12	36.74909375844908	39.0	35.0	39.0	33.0	39.0
13	36.67246127194256	39.0	35.0	39.0	32.0	39.0
14	37.78652503930385	40.0	37.0	41.0	33.0	41.0
15	37.78962178184112	40.0	37.0	41.0	33.0	41.0
16	37.7320754433574	40.0	36.0	41.0	33.0	41.0
17	37.67121206052292	39.0	36.0	41.0	33.0	41.0
18	37.56127144187537	39.0	36.0	41.0	33.0	41.0
19	37.58845618497342	39.0	36.0	41.0	32.0	41.0
20	37.48517969618377	39.0	36.0	41.0	32.0	41.0
21	37.42310465337513	39.0	35.0	41.0	32.0	41.0
22	37.40142495218449	39.0	35.0	41.0	32.0	41.0
23	37.24822256491393	39.0	35.0	41.0	32.0	41.0
24	37.121263631174706	39.0	35.0	41.0	31.0	41.0
25	37.17171775332205	39.0	35.0	41.0	32.0	41.0
26	37.07243423490181	39.0	35.0	41.0	32.0	41.0
27	37.04887195457777	39.0	35.0	41.0	32.0	41.0
28	36.978495538888275	39.0	35.0	41.0	31.0	41.0
29	36.74339845588456	39.0	35.0	40.0	31.0	41.0
30	36.74225939537166	39.0	35.0	40.0	31.0	41.0
31	36.583038763105456	39.0	35.0	40.0	30.0	41.0
32	36.52271111422649	39.0	35.0	40.0	30.0	41.0
33	36.41070266264783	39.0	35.0	40.0	30.0	41.0
34	36.27224547630254	39.0	35.0	40.0	30.0	41.0
35	36.16615012567217	39.0	35.0	40.0	30.0	41.0
36	36.04072829776794	38.0	35.0	40.0	30.0	41.0
37	35.988311486736826	38.0	35.0	40.0	29.0	41.0
38	35.850620349879335	38.0	35.0	40.0	29.0	41.0
39	35.75563021339235	38.0	35.0	40.0	29.0	41.0
40	35.64932958152669	38.0	35.0	40.0	28.0	41.0
41	35.53211149274506	38.0	34.0	40.0	28.0	41.0
42	35.40144748305178	38.0	34.0	40.0	27.0	41.0
43	35.24799224938165	38.0	34.0	40.0	26.0	41.0
44	35.177908734966906	38.0	34.0	40.0	26.0	41.0
45	34.94423860689144	37.0	34.0	40.0	26.0	41.0
46	34.76572904879685	37.0	34.0	40.0	25.0	41.0
47	34.59976918378178	37.0	33.0	40.0	24.0	41.0
48	34.529798323703474	37.0	33.0	40.0	24.0	41.0
49	34.3607917847451	36.0	33.0	40.0	24.0	41.0
50	34.27356979061314	36.0	33.0	40.0	24.0	41.0
51	32.9469848692709	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	5.0
13	4.0
14	14.0
15	29.0
16	58.0
17	138.0
18	284.0
19	547.0
20	910.0
21	1338.0
22	1833.0
23	2444.0
24	2990.0
25	3526.0
26	3892.0
27	4453.0
28	5353.0
29	6478.0
30	8174.0
31	10419.0
32	13365.0
33	18202.0
34	28457.0
35	33900.0
36	37539.0
37	53775.0
38	78987.0
39	82316.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.262880145799745	32.032885052521955	18.178654757017114	28.525580044661186
2	24.87908434555341	30.366101559136016	18.586713797903126	26.16810029740745
3	21.09189589737941	33.20023432101979	18.78598680191863	26.921882979682167
4	20.527622843295315	33.22677067582588	18.415228863543053	27.83037761733575
5	18.264021709742348	34.455203628971695	18.151617716271293	29.12915694501467
6	20.390184552837386	36.962138129237054	18.959224137067782	23.688453180857774
7	82.16807025625107	7.315271922533871	6.203248450377016	4.313409370838048
8	83.83260066290819	6.620319838178304	4.658882669256882	4.888196829656629
9	77.3689955238677	9.262439542172777	8.64959995193415	4.7189649820253745
10	36.63794398325706	31.981564743698865	13.198081371478926	18.182409901565144
11	28.61094699738642	30.215895777214786	23.40781871163494	17.765338513763858
12	26.445480308021992	26.55738361555331	27.584791163894533	19.412344912530166
13	26.02715720537136	27.20877602315172	25.431591280053674	21.33247549142325
14	21.377787568969488	28.941900403552868	28.483272082753373	21.19703994472427
15	20.48306179465868	29.741245506343688	27.558004466118586	22.217688232879045
16	21.614361675495427	29.3281796060603	26.439972762684878	22.61748595575939
17	21.37453311036119	29.525950552256592	27.295895376666035	21.80362096071618
18	24.172115798644143	26.749897359382352	25.085867638664972	23.992119203308533
19	22.378909105474502	31.80882809448945	24.10953005617696	21.702732743859087
20	25.758539198702223	29.2465677978831	24.10452319677959	20.89036980663509
21	23.107657490762346	31.35045011665982	24.490802399287023	21.05108999329081
22	22.448254108128136	28.01237695643031	25.8859637703654	23.653405165076155
23	22.399437229003734	29.880185854620834	24.49505822977479	23.225318686600644
24	21.088891781740983	29.4010294102921	26.499554389513634	23.010524418453283
25	23.25961567347266	29.727726985970783	22.67932066931696	24.333336671239596
26	24.84003084225389	28.166087539929702	23.59507525309674	23.398806364719665
27	21.740033846369528	27.3890229614572	24.989485595265513	25.881457596907765
28	23.00426584420656	29.20025434845739	24.720116559686772	23.075363247649282
29	22.294042838689005	30.387631054544727	24.916385448063846	22.401940658702422
30	24.592191302083855	26.776934400128177	27.22029179976568	21.41058249802229
31	24.889348407318028	28.168340626658523	24.07297998257613	22.869330983447323
32	25.087369696484185	25.453871804371992	27.403542853709585	22.055215645434245
33	24.82375854921242	26.57866276799215	24.353113765859227	24.244464916936202
34	22.10353183861891	26.0884912329892	28.630223406066314	23.177753522325588
35	25.122167369295934	24.849043189169162	28.588916816037973	21.439872625496932
36	22.982486005827983	29.950782572123806	25.97859066921683	21.08814075283138
37	24.06296626378138	27.82637212981785	24.138820183651603	23.971841422749165
38	24.7318826792706	28.083975045812764	26.33182459970159	20.852317675215044
39	24.15133733214504	24.735137137878894	25.281886184072178	25.831639345903888
40	27.825370757938376	23.658662367443398	24.848292160259554	23.66767471435867
41	24.596697475541493	22.707859767882	27.15094679711204	25.544495959464463
42	27.1088891781741	24.588436157535824	25.47189649820254	22.83077816608754
43	22.671059351311296	24.0612138629923	27.57177332946136	25.695953456235042
44	25.907743608743978	21.702732743859087	26.648258113615654	25.74126553378128
45	26.867308212250784	22.124310305118012	26.62197210177944	24.386409380851767
46	22.326837767741807	23.0896327969318	28.96518229975066	25.618347135575736
47	23.37577481149174	26.17836435917207	26.33633077315923	24.10953005617696
48	22.925658151667786	24.384907323032554	29.114887395732154	23.57454712956751
49	24.56740734806685	23.61985920711375	27.39828565134234	24.414447793477066
50	22.0131580264963	23.385287844346756	27.601313799905867	27.000240329251074
51	21.66693369916786	22.651782942631403	29.656629582528065	26.024653775672675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2702.0
1	2135.0
2	1568.0
3	1119.5
4	671.0
5	700.5
6	730.0
7	801.5
8	873.0
9	979.5
10	1086.0
11	1244.0
12	1402.0
13	1434.0
14	1466.0
15	1472.5
16	1479.0
17	1450.0
18	1421.0
19	1583.0
20	1745.0
21	1596.5
22	1448.0
23	1672.0
24	1896.0
25	2123.0
26	2816.5
27	3283.0
28	3878.0
29	4473.0
30	4594.0
31	4715.0
32	5934.0
33	7153.0
34	7827.0
35	8501.0
36	9168.5
37	9836.0
38	10048.5
39	10261.0
40	11655.0
41	13049.0
42	15768.0
43	18487.0
44	21011.0
45	23535.0
46	25986.0
47	28437.0
48	32938.0
49	37439.0
50	36529.0
51	35619.0
52	32839.5
53	30060.0
54	28084.5
55	26109.0
56	24449.5
57	22790.0
58	21326.5
59	19863.0
60	18694.5
61	17526.0
62	16272.5
63	15019.0
64	13338.0
65	11657.0
66	9659.5
67	7662.0
68	7116.0
69	6570.0
70	5633.5
71	4697.0
72	4416.0
73	4135.0
74	3574.5
75	2504.5
76	1995.0
77	1684.0
78	1373.0
79	974.5
80	576.0
81	507.5
82	439.0
83	331.5
84	224.0
85	136.5
86	49.0
87	51.0
88	53.0
89	31.0
90	9.0
91	7.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	399452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.232097766197903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37423520747429	19.247601114824654
2	11.437002974783038	6.0003316037360985
3	4.162426551151801	3.2756754070329595
4	2.0004337052562295	2.099022901243157
5	1.1758459746995185	1.5422453283154016
6	0.7622081106006545	1.1996590605279214
7	0.5469841139142655	1.0043978526929298
8	0.4035112981982031	0.846795825928056
9	0.316830879271912	0.7480024740370118
>10	4.099870710031214	26.531346066552814
>50	1.489503450813688	27.043082117633745
>100	0.22516908353437692	8.458803857441781
>500	0.0029889701354120827	0.5658368716626435
>1k	0.0029889701354120827	1.4371995183708437
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	2226	0.557263450927771	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2205	0.5520062485605279	No Hit
AAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT	1068	0.2673662918197931	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTT	991	0.24808988313990166	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	627	0.15696504210768752	No Hit
CGCAACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	547	0.13693760451818993	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	493	0.12341908414527902	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT	488	0.12216736929593544	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	421	0.10539439031473118	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.641378688803661	0.0
2	0.0	0.0	0.0	0.9863513012827574	0.0
3	0.0	0.0	0.0	1.357109239658332	0.0
4	0.0	0.0	0.0	3.0802199012647327	0.0
5	0.0	0.0	0.0	3.3648598580054676	0.0
6	0.0	0.0	0.0	4.908975296155734	0.0
7	0.0	0.0	0.0	6.002223045572435	0.0
8	0.0	0.0	0.0	6.788049627990347	0.0
9	0.0	0.0	0.0	7.778156073821135	0.0
10	0.0	0.0	0.0	9.436678249201407	0.0
11	0.0	0.0	0.0	10.893674333837357	0.0
12	0.0	0.0	0.0	11.612909686270191	0.0
13	0.0	0.0	0.0	11.935101088491233	0.0
14	0.0	0.0	0.0	12.251534602405295	0.0
15	0.0	0.0	0.0	12.534171815387081	0.0
16	0.0	0.0	0.0	13.147261748595575	0.0
17	0.0	0.0	0.0	13.841212461071668	0.0
18	0.0	0.0	0.0	14.766229734736589	0.0
19	0.0	0.0	0.0	15.161521284159297	0.0
20	0.0	0.0	0.0	15.627159208115117	0.0
21	0.0	0.0	0.0	16.069264892903277	0.0
22	0.0	0.0	0.0	16.547419965352532	0.0
23	0.0	0.0	0.0	17.00379519942321	0.0
24	0.0	0.0	0.0	17.35978290257653	0.0
25	0.0	0.0	0.0	17.65143246247359	0.0
26	0.0	0.0	0.0	17.93281796060603	0.0
27	0.0	0.0	0.0	18.309333787288587	0.0
28	0.0	0.0	0.0	18.651051941159388	0.0
29	0.0	0.0	0.0	19.00278381382494	0.0
30	0.0	0.0	0.0	19.493205691797762	0.0
31	0.0	0.0	0.0	19.80488268928432	0.0
32	0.0	0.0	0.0	20.154611818190922	0.0
33	0.0	0.0	0.0	20.494076885332905	0.0
34	0.0	0.0	0.0	20.818521374282767	0.0
35	2.5034296986872014E-4	0.0	0.0	21.15072649529856	0.0
36	2.5034296986872014E-4	0.0	0.0	21.442626398165487	0.0
37	2.5034296986872014E-4	0.0	0.0	21.75505442456165	0.0
38	5.006859397374403E-4	0.0	0.0	22.09577120655298	0.0
39	5.006859397374403E-4	0.0	0.0	22.51559636702282	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	7.027754E-4	45.0	18
TATTTCG	20	7.027754E-4	45.0	1
AACGTTC	20	7.027754E-4	45.0	26
TAGAGTT	25	3.88622E-5	45.0	37
AACGTAC	35	1.2092642E-7	45.0	26
AAACTAT	20	7.027754E-4	45.0	24
AACCGAT	20	7.027754E-4	45.0	36
CGAACTT	20	7.027754E-4	45.0	29
GCGACAC	20	7.027754E-4	45.0	21
AGTTTCG	20	7.027754E-4	45.0	20
CACGGTT	20	7.027754E-4	45.0	22
CCGATAA	20	7.027754E-4	45.0	38
ACGCACG	25	3.88622E-5	45.0	1
CGCATTC	25	3.88622E-5	45.0	25
CGCATAG	40	6.7957444E-9	45.0	1
CCGGATT	25	3.88622E-5	45.0	19
ACCGATA	20	7.027754E-4	45.0	37
GTCTCGA	20	7.027754E-4	45.0	31
TAAACGT	20	7.027754E-4	45.0	24
GCCGGAT	20	7.027754E-4	45.0	18
>>END_MODULE