SRR3548470_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230526
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	2489	1.0797046753945325	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT	2370	1.0280836001145206	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	2279	0.9886086601945118	TruSeq Adapter, Index 13 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	2191	0.9504350919202172	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1472	0.6385396874972888	No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	837	0.3630826891543687	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	660	0.2863017620572083	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	636	0.2758907888914916	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	538	0.2333793151314819	No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	520	0.2255710852571944	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	435	0.18869888862861456	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC	419	0.1817582398514701	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT	380	0.16484040845718054	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	331	0.14358467157717567	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	323	0.14011434718860344	No Hit
TTAGCTGGGGGGAATTTGGCCCCTCCTTAAATAAAAAAAAATCATCCTTTA	285	0.1236303063428854	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	0.12102756305145622	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	268	0.11625586701716943	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	262	0.11365312372574027	TruSeq Adapter, Index 13 (95% over 21bp)
CGCGACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	248	0.10758005604573889	No Hit
AGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	234	0.10150698836573749	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGC	30	2.1579272E-6	45.000004	20
ATGGGAC	30	2.1579272E-6	45.000004	5
ACCGAAC	30	2.1579272E-6	45.000004	32
TCGGGCA	30	2.1579272E-6	45.000004	5
GTGGCGA	30	2.1579272E-6	45.000004	28
CCGTCAT	30	2.1579272E-6	45.000004	28
AGACGTG	30	2.1579272E-6	45.000004	24
TACGCGG	30	2.1579272E-6	45.000004	2
TCTAAAC	30	2.1579272E-6	45.000004	34
GTACTGG	30	2.1579272E-6	45.000004	2
CTACCGA	30	2.1579272E-6	45.000004	30
TCGTAGC	30	2.1579272E-6	45.000004	23
ACATGCG	30	2.1579272E-6	45.000004	1
GAACCCG	30	2.1579272E-6	45.000004	18
TGCAGGT	30	2.1579272E-6	45.000004	32
CGTCATG	30	2.1579272E-6	45.000004	29
TGCGTTC	30	2.1579272E-6	45.000004	24
TACCGAA	30	2.1579272E-6	45.000004	31
TGATCAG	25	3.881074E-5	45.0	14
ATTTATG	20	7.021543E-4	45.0	43

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content