##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230526
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.665721003270782	31.0	31.0	34.0	30.0	34.0
2	31.84731006480831	33.0	31.0	34.0	30.0	34.0
3	32.01808906587543	33.0	31.0	34.0	30.0	34.0
4	35.69919228199856	37.0	35.0	37.0	33.0	37.0
5	35.43490539028136	37.0	35.0	37.0	33.0	37.0
6	34.773791242636406	37.0	35.0	37.0	32.0	37.0
7	35.104118407468135	37.0	35.0	37.0	32.0	37.0
8	35.54390394142092	37.0	35.0	37.0	33.0	37.0
9	37.207651197695704	39.0	37.0	39.0	34.0	39.0
10	36.82545135906579	39.0	37.0	39.0	32.0	39.0
11	36.82456208844122	39.0	37.0	39.0	32.0	39.0
12	36.68309865264656	39.0	35.0	39.0	32.0	39.0
13	36.65776962251547	39.0	35.0	39.0	32.0	39.0
14	37.75975811839012	40.0	37.0	41.0	33.0	41.0
15	37.830292461587845	40.0	37.0	41.0	33.0	41.0
16	37.66025524235878	39.0	36.0	41.0	32.0	41.0
17	37.65400865845935	39.0	36.0	41.0	33.0	41.0
18	37.5679315999063	39.0	36.0	41.0	32.0	41.0
19	37.50777786453589	39.0	36.0	41.0	32.0	41.0
20	37.48930706297771	39.0	36.0	41.0	32.0	41.0
21	37.449623903594386	39.0	36.0	41.0	32.0	41.0
22	37.445385769934845	39.0	36.0	41.0	32.0	41.0
23	37.32191162818945	39.0	36.0	41.0	32.0	41.0
24	37.1919826830813	39.0	35.0	41.0	32.0	41.0
25	37.25212340473526	39.0	35.0	41.0	32.0	41.0
26	37.20296625977113	39.0	35.0	41.0	32.0	41.0
27	37.07826015286779	39.0	35.0	41.0	31.0	41.0
28	37.02715528834058	39.0	35.0	41.0	31.0	41.0
29	36.775999236528634	39.0	35.0	40.0	31.0	41.0
30	36.78540381562166	39.0	35.0	40.0	31.0	41.0
31	36.783976644716866	39.0	35.0	40.0	31.0	41.0
32	36.63030200497992	39.0	35.0	40.0	31.0	41.0
33	36.466745616546504	39.0	35.0	40.0	30.0	41.0
34	36.45282527784285	39.0	35.0	40.0	30.0	41.0
35	36.41389691401404	39.0	35.0	40.0	30.0	41.0
36	36.34844659604556	39.0	35.0	40.0	30.0	41.0
37	36.26543209876543	39.0	35.0	40.0	30.0	41.0
38	36.08828071453979	38.0	35.0	40.0	30.0	41.0
39	36.01543860562366	38.0	35.0	40.0	30.0	41.0
40	35.920815873263756	38.0	35.0	40.0	29.0	41.0
41	35.9152156372817	38.0	35.0	40.0	30.0	41.0
42	35.80861594787573	38.0	35.0	40.0	29.0	41.0
43	35.71099138491971	38.0	35.0	40.0	29.0	41.0
44	35.609709967639226	38.0	35.0	40.0	29.0	41.0
45	35.44253142812524	38.0	34.0	40.0	28.0	41.0
46	35.17150343128324	38.0	34.0	40.0	27.0	41.0
47	35.10823941767957	37.0	34.0	40.0	27.0	41.0
48	35.01860093872275	37.0	34.0	40.0	26.0	41.0
49	34.91292088527975	37.0	34.0	40.0	26.0	41.0
50	34.84861143645402	37.0	34.0	40.0	26.0	41.0
51	33.50599064747577	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	1.0
14	6.0
15	14.0
16	28.0
17	68.0
18	125.0
19	250.0
20	429.0
21	710.0
22	1034.0
23	1212.0
24	1434.0
25	1765.0
26	1903.0
27	2237.0
28	2647.0
29	3464.0
30	4553.0
31	5817.0
32	7848.0
33	10767.0
34	16093.0
35	20507.0
36	23110.0
37	32118.0
38	46324.0
39	46045.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.018132444930288	35.25329030131091	17.856554141398366	26.872023112360427
2	21.820098383696415	35.669729227939584	18.61308485810711	23.89708753025689
3	19.960872092518848	31.94650494955016	22.82085317925093	25.271769778680063
4	23.600808585582538	31.445910656498615	18.64128124376426	26.31199951415459
5	17.66742146222118	36.564205339094066	18.121166376027	27.64720682265775
6	19.712310108187364	35.78164718947103	22.305076216999385	22.200966485342217
7	75.97104014297736	10.21359846611662	8.07240831836756	5.742953072538456
8	77.72095121591491	6.226195743647137	6.233136392424282	9.819716648013673
9	72.62521364184518	9.621474367316484	11.662025107796952	6.091286883041392
10	34.06470419822492	30.97481412074994	15.369632926437799	19.590848754587334
11	25.102157674188597	29.083487328978073	26.16277556544598	19.651579431387347
12	25.1173403433886	24.639303158862774	31.142691063047117	19.100665434701508
13	25.187180621708617	27.06809644031476	26.17188516696598	21.572837771010644
14	18.787902449181438	27.662389491857752	31.264586207195716	22.285121851765094
15	18.157170991558434	30.395269947858377	27.55177290197201	23.895786158611177
16	18.086029341592706	30.165360957115467	27.804239001240642	23.944370700051188
17	18.508107545352804	28.796317985823727	28.950313630566615	23.745260838256858
18	21.77021247061069	26.357547521754597	25.576724534325844	26.295515473308868
19	20.476649054770395	31.959084875458732	27.114512029011912	20.44975404075896
20	23.73268091234828	27.855860076520656	26.014419197834513	22.397039813296548
21	21.363316936050598	30.502416213355545	25.266998082645774	22.867268767948083
22	21.050120159981955	28.75771062700086	25.710765813834445	24.48140339918274
23	21.936788041262155	29.15376139784666	23.21473499735388	25.6947155635373
24	17.97281000841554	31.558696199127212	25.171130371411465	25.29736342104578
25	19.67543791155878	28.75380651206372	25.910743256725922	25.66001231965158
26	24.07797818901122	28.67355526057798	23.512749104222515	23.735717446188282
27	18.844729011044308	28.74903481602943	25.4387791398801	26.967457033046166
28	21.126901087079116	27.185653678977644	27.743074533892052	23.944370700051188
29	23.655466194702548	30.508055490486974	23.136652698611	22.699825616199472
30	21.282198103467724	28.909971109549463	24.706540693891363	25.10129009309145
31	24.073640283525503	28.145198372417862	22.464277348325133	25.316883995731498
32	23.487589252405368	29.176318506372382	22.956195830405246	24.379896410817
33	22.301172102062242	25.253116785091485	25.099554930897167	27.346156181949105
34	20.04025576290744	27.45677277183485	28.630609996269403	23.872361468988313
35	24.592887570165622	27.15919245551478	24.883093447159972	23.364826527159625
36	22.401811509330834	28.132618446509287	26.476406132063197	22.989163912096682
37	23.594301727353965	29.114286457926653	22.095989172587906	25.195422642131472
38	22.78094444878235	28.160381041617867	24.945993076702845	24.11268143289694
39	21.684321941993527	25.306473022565783	26.522821720760348	26.48638331468034
40	25.402774524348665	25.14293398575432	25.885583404908775	23.568708084988245
41	22.518067376348007	22.4495284696737	29.861273782566826	25.171130371411465
42	26.017021941125947	24.241517225822683	26.105515213034536	23.635945620016834
43	23.497566435022513	23.357885878382483	26.840790192863277	26.303757493731727
44	22.541925856519438	24.34172284254271	25.614898102600144	27.501453198337718
45	26.57140626220036	23.34010046589105	25.33640457041722	24.752088701491374
46	19.352697743421565	23.893617205868317	31.084129338989964	25.669555711720154
47	22.29032733834795	25.1173403433886	25.743300104977312	26.84903221328614
48	21.77932207213069	23.136218908062432	32.15992989944735	22.924529120359527
49	23.62379948465683	21.343796361364877	28.04325759350355	26.98914656047474
50	20.970302699044794	22.415259016336552	26.92971725532044	29.68472102929821
51	20.451489202953248	21.1377458507934	31.677988599984385	26.732776346268967
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1739.0
1	1391.5
2	1044.0
3	744.5
4	445.0
5	415.0
6	385.0
7	420.5
8	456.0
9	452.5
10	449.0
11	499.0
12	549.0
13	550.5
14	552.0
15	619.5
16	687.0
17	695.5
18	704.0
19	750.0
20	796.0
21	848.5
22	901.0
23	982.0
24	1063.0
25	1227.0
26	1576.0
27	1761.0
28	1935.5
29	2110.0
30	2673.0
31	3236.0
32	3210.0
33	3184.0
34	3747.5
35	4311.0
36	4662.5
37	5014.0
38	5712.5
39	6411.0
40	6599.0
41	6787.0
42	8551.5
43	10316.0
44	15018.5
45	19721.0
46	21048.0
47	22375.0
48	23396.0
49	24417.0
50	23167.0
51	21917.0
52	19976.5
53	18036.0
54	16399.5
55	14763.0
56	13670.5
57	12578.0
58	11070.5
59	9563.0
60	8934.0
61	8305.0
62	7726.5
63	7148.0
64	6024.0
65	4900.0
66	4286.0
67	3672.0
68	3272.5
69	2873.0
70	2281.5
71	1690.0
72	1566.0
73	1442.0
74	1178.5
75	1022.0
76	1129.0
77	815.5
78	502.0
79	349.5
80	197.0
81	123.0
82	49.0
83	36.0
84	23.0
85	15.0
86	7.0
87	7.5
88	8.0
89	5.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	230526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.802373701881784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79893676108863	25.519897972462974
2	9.608823296040626	6.303844251841441
3	3.390727075564019	3.336716899612191
4	1.61205004099553	2.115162714834769
5	0.9243830833928429	1.5160979672574892
6	0.6638631013779788	1.3065771322974415
7	0.5170726546589437	1.1872847314402715
8	0.3887963183369039	1.0202753702402332
9	0.31870718611970694	0.9408916998516436
>10	3.940860641646169	30.391799623469808
>50	0.7405644158798169	15.89538707130649
>100	0.08199106032955115	4.3964672097724335
>500	0.006612182284641223	1.384225640491745
>1k	0.006612182284641223	4.685371715121071
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	2489	1.0797046753945325	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT	2370	1.0280836001145206	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	2279	0.9886086601945118	TruSeq Adapter, Index 13 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	2191	0.9504350919202172	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1472	0.6385396874972888	No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	837	0.3630826891543687	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	660	0.2863017620572083	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	636	0.2758907888914916	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	538	0.2333793151314819	No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	520	0.2255710852571944	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	435	0.18869888862861456	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC	419	0.1817582398514701	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT	380	0.16484040845718054	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	331	0.14358467157717567	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	323	0.14011434718860344	No Hit
TTAGCTGGGGGGAATTTGGCCCCTCCTTAAATAAAAAAAAATCATCCTTTA	285	0.1236303063428854	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	279	0.12102756305145622	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	268	0.11625586701716943	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	262	0.11365312372574027	TruSeq Adapter, Index 13 (95% over 21bp)
CGCGACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	248	0.10758005604573889	No Hit
AGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	234	0.10150698836573749	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0870791147202485	0.0
2	0.0	0.0	0.0	1.525641359326063	0.0
3	0.0	0.0	0.0	2.223610351977651	0.0
4	0.0	0.0	0.0	5.239756036195484	0.0
5	0.0	0.0	0.0	5.666172145441295	0.0
6	0.0	0.0	0.0	7.695010541110331	0.0
7	0.0	0.0	0.0	9.29916798972784	0.0
8	0.0	0.0	0.0	10.429626159305242	0.0
9	0.0	0.0	0.0	12.713533397534334	0.0
10	0.0	0.0	0.0	14.97228078394628	0.0
11	0.0	0.0	0.0	17.704727449398334	0.0
12	0.0	0.0	0.0	18.650824635832834	0.0
13	0.0	0.0	0.0	19.10717229293008	0.0
14	0.0	0.0	0.0	19.66589451949021	0.0
15	0.0	0.0	0.0	20.065415614724586	0.0
16	0.0	0.0	0.0	20.85578199422191	0.0
17	0.0	0.0	0.0	21.728134787399252	0.0
18	0.0	0.0	0.0	23.296721411033896	0.0
19	0.0	0.0	0.0	23.77736133885115	0.0
20	0.0	0.0	0.0	24.330010497731276	0.0
21	0.0	0.0	0.0	24.863139081925684	0.0
22	0.0	0.0	0.0	25.38498911185723	0.0
23	0.0	0.0	0.0	25.875172431743056	0.0
24	0.0	0.0	0.0	26.238688911445998	0.0
25	0.0	0.0	0.0	26.55795875519464	0.0
26	0.0	0.0	0.0	26.880265132783286	0.0
27	0.0	0.0	0.0	27.248987099069087	0.0
28	0.0	0.0	0.0	27.598622281217736	0.0
29	0.0	0.0	0.0	27.930038260326384	0.0
30	0.0	0.0	0.0	28.486157743595083	0.0
31	0.0	0.0	0.0	28.836660506840875	0.0
32	0.0	0.0	0.0	29.17545092527524	0.0
33	0.0	0.0	0.0	29.513807553161033	0.0
34	0.0	0.0	0.0	29.835246349652532	0.0
35	0.0	0.0	0.0	30.17186781534404	0.0
36	0.0	0.0	0.0	30.462941273435533	0.0
37	0.0	0.0	0.0	30.773535306212747	0.0
38	0.0	0.0	0.0	31.087165872829964	0.0
39	0.0	0.0	0.0	31.425522500715754	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGC	30	2.1579272E-6	45.000004	20
ATGGGAC	30	2.1579272E-6	45.000004	5
ACCGAAC	30	2.1579272E-6	45.000004	32
TCGGGCA	30	2.1579272E-6	45.000004	5
GTGGCGA	30	2.1579272E-6	45.000004	28
CCGTCAT	30	2.1579272E-6	45.000004	28
AGACGTG	30	2.1579272E-6	45.000004	24
TACGCGG	30	2.1579272E-6	45.000004	2
TCTAAAC	30	2.1579272E-6	45.000004	34
GTACTGG	30	2.1579272E-6	45.000004	2
CTACCGA	30	2.1579272E-6	45.000004	30
TCGTAGC	30	2.1579272E-6	45.000004	23
ACATGCG	30	2.1579272E-6	45.000004	1
GAACCCG	30	2.1579272E-6	45.000004	18
TGCAGGT	30	2.1579272E-6	45.000004	32
CGTCATG	30	2.1579272E-6	45.000004	29
TGCGTTC	30	2.1579272E-6	45.000004	24
TACCGAA	30	2.1579272E-6	45.000004	31
TGATCAG	25	3.881074E-5	45.0	14
ATTTATG	20	7.021543E-4	45.0	43
>>END_MODULE