SRR3548469_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237213
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	2810	1.184589377479312	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT	2784	1.1736287640222078	No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	2504	1.0555913883303192	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	2463	1.0383073440325783	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1436	0.6053631124769722	No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	946	0.3987977050161669	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	734	0.30942654913516543	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	675	0.2845543878286603	No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	566	0.2386041237200322	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	539	0.22722194820688582	No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	482	0.2031929110124656	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT	468	0.19729104222787117	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC	438	0.18464418054659734	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	377	0.15892889512800734	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	351	0.14796828167090337	No Hit
TTAGCTGGGGGGAATTTGGCCCCTCCTTAAATAAAAAAAAATCATCCTTTA	340	0.1433310990544363	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	310	0.1306842373731625	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	270	0.11382175513146413	No Hit
CGCGACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	258	0.1087630104589546	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	255	0.10749832429082723	No Hit
AACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	238	0.1003317693381054	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.021958E-4	45.0	1
ATTTAGT	25	3.881418E-5	45.0	32
ATGGGAC	25	3.881418E-5	45.0	5
TCGTTGC	20	7.021958E-4	45.0	16
CTCACGT	20	7.021958E-4	45.0	45
TACCCGT	20	7.021958E-4	45.0	22
CTCGTTG	20	7.021958E-4	45.0	15
AATCATC	55	1.8189894E-12	45.0	40
CATATAC	20	7.021958E-4	45.0	23
CAACGTT	20	7.021958E-4	45.0	43
CGAAATG	20	7.021958E-4	45.0	20
GAATGCC	35	1.2064811E-7	45.0	10
CGAAACC	25	3.881418E-5	45.0	39
ACGTTGT	20	7.021958E-4	45.0	45
TTAGCCC	20	7.021958E-4	45.0	23
CTTCATA	35	1.2064811E-7	45.0	20
GCTATTG	20	7.021958E-4	45.0	35
TATGGCG	25	3.881418E-5	45.0	1
CGGTTCA	20	7.021958E-4	45.0	7
CGTTGGG	40	6.7757355E-9	45.0	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content