##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237213
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654757538583468	31.0	31.0	34.0	30.0	34.0
2	31.839515540885195	33.0	31.0	34.0	30.0	34.0
3	31.9972303372918	33.0	31.0	34.0	30.0	34.0
4	35.67610965672202	37.0	35.0	37.0	33.0	37.0
5	35.431797582763174	37.0	35.0	37.0	33.0	37.0
6	35.44333152061649	37.0	35.0	37.0	33.0	37.0
7	35.4405829360111	37.0	35.0	37.0	33.0	37.0
8	35.53817033636437	37.0	35.0	37.0	33.0	37.0
9	37.225017178653786	39.0	37.0	39.0	34.0	39.0
10	36.824655478409696	39.0	37.0	39.0	32.0	39.0
11	36.82695299161513	39.0	37.0	39.0	32.0	39.0
12	36.69225969908901	39.0	35.0	39.0	32.0	39.0
13	36.67454144587354	39.0	35.0	39.0	32.0	39.0
14	37.77205718067728	40.0	37.0	41.0	33.0	41.0
15	37.847521004329444	40.0	37.0	41.0	33.0	41.0
16	37.69654276957839	39.0	36.0	41.0	33.0	41.0
17	37.65702132682441	39.0	36.0	41.0	33.0	41.0
18	37.519233768806934	39.0	36.0	41.0	32.0	41.0
19	37.50389312558755	39.0	36.0	41.0	32.0	41.0
20	37.482262776492014	39.0	36.0	41.0	32.0	41.0
21	37.45658543165847	39.0	36.0	41.0	32.0	41.0
22	37.429942709716585	39.0	36.0	41.0	32.0	41.0
23	37.26737994966549	39.0	36.0	40.0	32.0	41.0
24	37.14005556187899	39.0	35.0	40.0	31.0	41.0
25	37.213251381669636	39.0	35.0	41.0	32.0	41.0
26	37.145830962046766	39.0	35.0	41.0	32.0	41.0
27	37.08091462103679	39.0	35.0	41.0	31.0	41.0
28	37.00539177869678	39.0	35.0	41.0	31.0	41.0
29	36.783536315463316	39.0	35.0	40.0	31.0	41.0
30	36.7779885588058	39.0	35.0	40.0	31.0	41.0
31	36.761252545180916	39.0	35.0	40.0	31.0	41.0
32	36.5998532964045	39.0	35.0	40.0	31.0	41.0
33	36.60513125334615	39.0	35.0	40.0	31.0	41.0
34	36.5200684616779	39.0	35.0	40.0	30.0	41.0
35	36.45476428357637	39.0	35.0	40.0	30.0	41.0
36	36.341305071813096	39.0	35.0	40.0	30.0	41.0
37	36.27431464548739	39.0	35.0	40.0	30.0	41.0
38	36.182338236100044	38.0	35.0	40.0	30.0	41.0
39	36.05490845779953	38.0	35.0	40.0	30.0	41.0
40	35.923486486828295	38.0	35.0	40.0	29.0	41.0
41	35.8766804517459	38.0	35.0	40.0	29.0	41.0
42	35.74382095416356	38.0	35.0	40.0	29.0	41.0
43	35.660499213786764	38.0	35.0	40.0	28.0	41.0
44	35.56950082836944	38.0	35.0	40.0	28.0	41.0
45	35.38852423771041	38.0	34.0	40.0	28.0	41.0
46	35.12668361346132	38.0	34.0	40.0	26.0	41.0
47	35.043564222871424	37.0	34.0	40.0	26.0	41.0
48	34.98090323886127	37.0	34.0	40.0	26.0	41.0
49	34.79477515987741	37.0	34.0	40.0	26.0	41.0
50	34.72964382221885	37.0	34.0	40.0	26.0	41.0
51	33.27786841361983	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	6.0
14	4.0
15	13.0
16	34.0
17	60.0
18	135.0
19	233.0
20	465.0
21	673.0
22	1010.0
23	1266.0
24	1536.0
25	1735.0
26	2029.0
27	2304.0
28	2935.0
29	3607.0
30	4569.0
31	6195.0
32	7952.0
33	10910.0
34	16498.0
35	20775.0
36	23481.0
37	32875.0
38	48733.0
39	47169.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.920493396230395	35.53093633148268	17.70771416406352	26.84085610822341
2	21.614751299465038	36.134613195735476	18.485917719517904	23.764717785281583
3	19.885082183522826	31.765544046911426	23.215422426258257	25.133951343307494
4	23.687571929025815	31.249973652371498	18.66213065894365	26.400323759659038
5	17.514638742396073	36.562920244674615	18.216539565706768	27.705901447222537
6	20.098814145936352	34.5343636309983	22.84402625488485	22.522795968180496
7	75.62064473700852	10.340495672665496	8.220460092827965	5.81839949749803
8	77.14796406605035	6.232794998587767	6.339028636710467	10.280212298651422
9	72.1912374111031	9.520979035718954	12.066792292159366	6.220991261018578
10	33.75531695143184	31.00841859425917	15.50378773507354	19.732476719235457
11	25.1082360578889	28.67170011761581	26.59592855366274	19.624135270832543
12	25.063128917892357	24.358698722245407	31.55855707739458	19.019615282467655
13	25.39363356982965	26.858140152521155	26.220738323784953	21.52748795386425
14	18.269656384768123	27.765341697124523	31.558135515338538	22.40686640276882
15	17.90669145451556	30.25972438272776	27.64140245264804	24.19218171010864
16	17.75535067639632	30.150539810212763	28.063807632802586	24.030301880588333
17	18.18070679094316	28.683925417241046	29.237436396824794	23.897931394990998
18	21.60505537217606	26.61447728412861	25.682403578218732	26.0980637654766
19	20.325192970031154	31.792945580554182	27.667117738066633	20.214743711348028
20	23.423674081943233	28.08067011504429	26.00405542697913	22.491600376033354
21	21.062083443993373	30.526151602146594	25.461926622908525	22.949838330951508
22	20.68984414850788	28.85803054638658	25.998575120250578	24.45355018485496
23	22.09533204335344	29.079772187864915	22.868476854135313	25.956418914646328
24	17.781909085926994	31.631065751033884	25.271802135633376	25.315223027405747
25	19.625821519056714	28.66411200060705	25.85018527652363	25.859881203812606
26	24.00247878488953	28.761071273496814	23.65511165071054	23.581338290903112
27	18.557161706989078	28.493379367909853	25.773882544379944	27.17557638072112
28	21.023299734837465	26.952570053074666	28.008583003461023	24.015547208626845
29	23.779050895187027	30.336448676927485	23.10792410196743	22.776576325918057
30	21.1248961903437	29.128251824309796	24.55135258185681	25.19549940348969
31	24.141594263383542	28.057905764017992	22.220957536054094	25.579542436544372
32	23.422409395775105	29.329758487098097	22.749596354331338	24.49823576279546
33	22.3828373655744	24.92274875323022	25.19170534498531	27.502708536210076
34	20.02335453790475	27.303309683701986	28.563358669212903	24.109977109180356
35	24.65379216147513	27.0373040263392	24.957738403881745	23.351165408303928
36	22.35037708725913	28.2615202370865	26.294511683592383	23.093590992061984
37	23.388684431291708	29.180947081315107	21.939775644673773	25.490592842719412
38	22.68088173919642	27.979073659538052	24.92317031528626	24.41687428597927
39	21.81710108636542	25.105706685552647	26.720289360195267	26.356902867886667
40	25.165990059566717	25.246508412270828	25.966957966047392	23.620543562115063
41	22.497502244817948	22.112194525595143	30.2630968791761	25.127206350410813
42	26.32950133424391	24.119673036469333	25.864939948485116	23.68588568080164
43	23.672395695008284	23.03035668365562	26.953413177186746	26.34383444414935
44	22.68636204592497	24.258366952907302	25.44295633038661	27.612314670781114
45	26.827787684486093	23.322077626437	25.321124896190344	24.529009792886562
46	19.337051510667628	23.684199432577472	31.377706955352362	25.601042101402538
47	22.38831767230295	25.04795268387483	25.805078136527086	26.758651507295134
48	21.844924182064222	22.760135405732402	32.3818677728455	23.013072639357876
49	23.747012178927797	21.098337780813026	28.121983196536448	27.03266684372273
50	20.949104812973992	22.14128230746207	26.862355773081575	30.047257106482363
51	20.40528976067922	20.860155219149036	31.702309738505058	27.03224528166669
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1717.0
1	1409.5
2	1102.0
3	769.0
4	436.0
5	392.5
6	349.0
7	392.0
8	435.0
9	453.5
10	472.0
11	516.0
12	560.0
13	568.0
14	576.0
15	658.5
16	741.0
17	740.0
18	739.0
19	765.5
20	792.0
21	835.5
22	879.0
23	950.5
24	1022.0
25	1224.5
26	1598.0
27	1769.0
28	1955.0
29	2141.0
30	2701.0
31	3261.0
32	3298.0
33	3335.0
34	3913.5
35	4492.0
36	4842.0
37	5192.0
38	5827.0
39	6462.0
40	6762.0
41	7062.0
42	8726.5
43	10391.0
44	15816.5
45	21242.0
46	22289.0
47	23336.0
48	24262.5
49	25189.0
50	23907.0
51	22625.0
52	20526.5
53	18428.0
54	16705.0
55	14982.0
56	13800.5
57	12619.0
58	11241.0
59	9863.0
60	9225.0
61	8587.0
62	7843.0
63	7099.0
64	6046.5
65	4994.0
66	4453.5
67	3913.0
68	3363.5
69	2814.0
70	2285.0
71	1756.0
72	1656.5
73	1557.0
74	1258.0
75	1050.0
76	1141.0
77	807.5
78	474.0
79	332.0
80	190.0
81	121.5
82	53.0
83	40.5
84	28.0
85	17.5
86	7.0
87	5.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	237213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.19638046818682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55911697698168	24.9712283896751
2	9.466572393746562	6.095787330373968
3	3.5483279650142716	3.4272995156251977
4	1.6903658313038468	2.176946457403262
5	1.005577814439469	1.6187982952030455
6	0.6311048262497709	1.2191574660747935
7	0.5119543300075943	1.1538153473882122
8	0.381019718752455	0.9813964664668462
9	0.29853091366171736	0.8650453389991274
>10	4.048498180008903	30.16402979600612
>50	0.7541833608296017	15.914810739714941
>100	0.09165422787859742	4.895600156821085
>500	0.006546730562756959	1.4586047139069107
>1k	0.006546730562756959	5.057479986341389
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	2810	1.184589377479312	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGT	2784	1.1736287640222078	No Hit
AATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	2504	1.0555913883303192	No Hit
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	2463	1.0383073440325783	TruSeq Adapter, Index 13 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1436	0.6053631124769722	No Hit
AAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	946	0.3987977050161669	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	734	0.30942654913516543	No Hit
AATGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTT	675	0.2845543878286603	No Hit
AACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	566	0.2386041237200322	TruSeq Adapter, Index 19 (95% over 21bp)
AATGATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	539	0.22722194820688582	No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	482	0.2031929110124656	TruSeq Adapter, Index 19 (95% over 22bp)
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACACTGTCGTAT	468	0.19729104222787117	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACACTGTC	438	0.18464418054659734	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	377	0.15892889512800734	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	351	0.14796828167090337	No Hit
TTAGCTGGGGGGAATTTGGCCCCTCCTTAAATAAAAAAAAATCATCCTTTA	340	0.1433310990544363	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	310	0.1306842373731625	TruSeq Adapter, Index 13 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	270	0.11382175513146413	No Hit
CGCGACGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	258	0.1087630104589546	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCT	255	0.10749832429082723	No Hit
AACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	238	0.1003317693381054	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1778443845826325	0.0
2	0.0	0.0	0.0	1.6436704565095506	0.0
3	0.0	0.0	0.0	2.369178754958624	0.0
4	0.0	0.0	0.0	5.57515819116153	0.0
5	0.0	0.0	0.0	6.030866773743429	0.0
6	0.0	0.0	0.0	8.158912032645766	0.0
7	0.0	0.0	0.0	9.762534093831283	0.0
8	0.0	0.0	0.0	10.935319733741405	0.0
9	0.0	0.0	0.0	13.427594609064426	0.0
10	0.0	0.0	0.0	15.845253000467935	0.0
11	0.0	0.0	0.0	18.801246137437662	0.0
12	0.0	0.0	0.0	19.790652282969315	0.0
13	0.0	0.0	0.0	20.260272413400614	0.0
14	0.0	0.0	0.0	20.87954707372699	0.0
15	0.0	0.0	0.0	21.29309945070464	0.0
16	0.0	0.0	0.0	22.099969225969907	0.0
17	0.0	0.0	0.0	23.01560201169413	0.0
18	0.0	0.0	0.0	24.600253780357736	0.0
19	0.0	0.0	0.0	25.112030116393285	0.0
20	0.0	0.0	0.0	25.71106979802962	0.0
21	0.0	0.0	0.0	26.312217289946165	0.0
22	0.0	0.0	0.0	26.812189888412522	0.0
23	0.0	0.0	0.0	27.345044327250193	0.0
24	0.0	0.0	0.0	27.71686206067964	0.0
25	0.0	0.0	0.0	28.060013574298203	0.0
26	0.0	0.0	0.0	28.377449802498177	0.0
27	0.0	0.0	0.0	28.751375346207837	0.0
28	0.0	0.0	0.0	29.094948421882442	0.0
29	0.0	0.0	0.0	29.44062930783726	0.0
30	0.0	0.0	0.0	30.025335879568154	0.0
31	0.0	0.0	0.0	30.341085859543956	0.0
32	0.0	0.0	0.0	30.67496300792958	0.0
33	0.0	0.0	0.0	31.010104842483337	0.0
34	0.0	0.0	0.0	31.309835464329527	0.0
35	0.0	0.0	0.0	31.650457605611834	0.0
36	0.0	0.0	0.0	31.970844768204103	0.0
37	0.0	0.0	0.0	32.289967244628244	0.0
38	0.0	0.0	0.0	32.62721688946221	0.0
39	0.0	0.0	0.0	32.97331933747307	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.021958E-4	45.0	1
ATTTAGT	25	3.881418E-5	45.0	32
ATGGGAC	25	3.881418E-5	45.0	5
TCGTTGC	20	7.021958E-4	45.0	16
CTCACGT	20	7.021958E-4	45.0	45
TACCCGT	20	7.021958E-4	45.0	22
CTCGTTG	20	7.021958E-4	45.0	15
AATCATC	55	1.8189894E-12	45.0	40
CATATAC	20	7.021958E-4	45.0	23
CAACGTT	20	7.021958E-4	45.0	43
CGAAATG	20	7.021958E-4	45.0	20
GAATGCC	35	1.2064811E-7	45.0	10
CGAAACC	25	3.881418E-5	45.0	39
ACGTTGT	20	7.021958E-4	45.0	45
TTAGCCC	20	7.021958E-4	45.0	23
CTTCATA	35	1.2064811E-7	45.0	20
GCTATTG	20	7.021958E-4	45.0	35
TATGGCG	25	3.881418E-5	45.0	1
CGGTTCA	20	7.021958E-4	45.0	7
CGTTGGG	40	6.7757355E-9	45.0	3
>>END_MODULE