FastQCFastQC Report
Sat 18 Jun 2016
SRR3548468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453442
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT45791.0098314668689712TruSeq Adapter, Index 13 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18380.4053440131262653No Hit
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT13840.3052209543888744TruSeq Adapter, Index 13 (95% over 23bp)
CGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC12320.27169957789529864No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT9940.21921215943825229No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT9160.20201040044812787TruSeq Adapter, Index 13 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC8720.19230684409472434TruSeq Adapter, Index 13 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC7700.16981223618456165No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT6890.15194887107943242No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT6040.13320336448763018No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6030.1329828291159619No Hit
ACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG5180.11423732252415966TruSeq Adapter, Index 13 (100% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4880.1076212613741118No Hit
GCCCTGGGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG4860.10718019063077526No Hit
TGAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT4660.10276948319741003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCACG302.1625838E-645.0000041
TAGCGAG302.1625838E-645.0000041
TTCACGG453.8380676E-1045.0000042
GCGCCTA351.2097553E-745.00000425
CGCGACG302.1625838E-645.0000041
ATACTAA302.1625838E-645.00000438
TGCATAG351.2097553E-745.0000041
CGCCTAG351.2097553E-745.00000426
AAACGCG302.1625838E-645.0000041
ACGATGA302.1625838E-645.00000430
CCACTAG351.2097553E-745.0000041
TAACACG351.2097553E-745.0000041
TATTACG302.1625838E-645.0000041
TAGGGTA700.045.0000045
CATACCG351.2097553E-745.0000041
GGGCCTA302.1625838E-645.0000047
GATTGGT351.2097553E-745.0000049
GCGTGGC302.1625838E-645.00000444
TCATACG453.8380676E-1045.0000041
TATACGG700.045.0000042
Produced by FastQC (version 0.11.5)