##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548468_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453442
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.610336051799347	31.0	31.0	34.0	30.0	34.0
2	31.814470207876642	33.0	31.0	34.0	30.0	34.0
3	32.00818406764261	33.0	31.0	34.0	30.0	34.0
4	35.70381658514209	37.0	35.0	37.0	33.0	37.0
5	35.47421500434454	37.0	35.0	37.0	33.0	37.0
6	34.80586050696671	37.0	35.0	37.0	32.0	37.0
7	35.21140961798863	37.0	35.0	37.0	33.0	37.0
8	35.630415797389745	37.0	35.0	37.0	33.0	37.0
9	37.245517618570844	39.0	37.0	39.0	34.0	39.0
10	36.83001795157925	39.0	37.0	39.0	32.0	39.0
11	36.874354382699444	39.0	37.0	39.0	33.0	39.0
12	36.73082996281774	39.0	35.0	39.0	33.0	39.0
13	36.68970673206275	39.0	35.0	39.0	33.0	39.0
14	37.78585133269525	40.0	37.0	41.0	33.0	41.0
15	37.78294688185038	40.0	36.0	41.0	33.0	41.0
16	37.70848752431402	39.0	36.0	41.0	33.0	41.0
17	37.696525685754736	39.0	36.0	41.0	33.0	41.0
18	37.65832013796693	39.0	36.0	41.0	33.0	41.0
19	37.63882260575774	39.0	36.0	41.0	32.0	41.0
20	37.54877801350558	39.0	36.0	41.0	32.0	41.0
21	37.518097132599095	39.0	36.0	41.0	32.0	41.0
22	37.517378187287456	39.0	36.0	41.0	32.0	41.0
23	37.4059438693372	39.0	36.0	41.0	32.0	41.0
24	37.27064982952616	39.0	35.0	41.0	32.0	41.0
25	37.31321977231928	39.0	35.0	41.0	32.0	41.0
26	37.2697434291486	39.0	35.0	41.0	32.0	41.0
27	37.191387211594865	39.0	35.0	41.0	32.0	41.0
28	37.05021149342143	39.0	35.0	41.0	31.0	41.0
29	36.81808037191085	39.0	35.0	40.0	31.0	41.0
30	36.849001636372456	39.0	35.0	40.0	31.0	41.0
31	36.7851963426414	39.0	35.0	40.0	31.0	41.0
32	36.74011450196497	39.0	35.0	40.0	31.0	41.0
33	36.510499689045126	39.0	35.0	40.0	30.0	41.0
34	36.464890768830415	39.0	35.0	40.0	30.0	41.0
35	36.44690831462458	39.0	35.0	40.0	30.0	41.0
36	36.36302768601056	39.0	35.0	40.0	30.0	41.0
37	36.36013426193427	39.0	35.0	40.0	30.0	41.0
38	36.190535945060226	38.0	35.0	40.0	30.0	41.0
39	36.219670873011324	39.0	35.0	40.0	30.0	41.0
40	36.19988664481896	38.0	35.0	40.0	30.0	41.0
41	36.20923954993141	38.0	35.0	40.0	30.0	41.0
42	36.127451801994525	38.0	35.0	40.0	30.0	41.0
43	36.02240859911521	38.0	35.0	40.0	30.0	41.0
44	35.90246823187971	38.0	35.0	40.0	29.0	41.0
45	35.68421981201565	38.0	35.0	40.0	29.0	41.0
46	35.552782935855085	38.0	34.0	40.0	28.0	41.0
47	35.482454205830074	38.0	34.0	40.0	28.0	41.0
48	35.401976438000894	38.0	34.0	40.0	28.0	41.0
49	35.356248428685475	38.0	34.0	40.0	27.0	41.0
50	35.27146581040133	38.0	34.0	40.0	27.0	41.0
51	34.0375174774282	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	9.0
13	4.0
14	13.0
15	19.0
16	24.0
17	84.0
18	175.0
19	386.0
20	722.0
21	1133.0
22	1554.0
23	2005.0
24	2427.0
25	2848.0
26	3407.0
27	4101.0
28	5317.0
29	6774.0
30	8921.0
31	11638.0
32	15448.0
33	21033.0
34	32070.0
35	39152.0
36	44573.0
37	62906.0
38	89434.0
39	97235.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.559189488402044	31.580224152151764	20.052840275051715	27.807746084394473
2	24.676584877448494	30.077496129604224	19.851050409975258	25.394868582972023
3	20.55213235650866	32.67694655545803	19.67550425412732	27.09541683390599
4	19.356610106695012	32.56292976830554	19.718288116230962	28.36217200876849
5	17.72045818428818	33.21196536712524	20.319908610141983	28.747667838444606
6	21.470882714878638	37.42970435028074	19.28846467684952	21.8109482579911
7	79.6822967435747	7.619938161881784	6.905844628419952	5.791920466123561
8	81.53038315815473	5.038571636504779	7.022728375404132	6.408316829936353
9	74.42142545242831	9.6821644223517	10.507628318506006	5.388781806713979
10	33.07170487074422	33.96906329806237	13.297841840852854	19.66138999034055
11	26.084482690178678	27.653150788855026	27.583020540664517	18.679345980301783
12	27.11195698678111	21.163235871401415	32.844994508669245	18.87981263314823
13	27.590959814044574	21.990243515157395	27.644329373988295	22.774467296809735
14	20.91138447695626	26.743442380723444	31.084901707384848	21.260271434935447
15	18.189757455198237	29.700821714794834	30.564217694876085	21.545203135130844
16	20.836402450589052	26.03177473634996	29.462422978021447	23.669399835039542
17	19.37248865345513	26.778728040190362	28.940636288654336	24.90814701770017
18	22.167774489350347	25.30797764653473	27.84391388534807	24.680333978766853
19	21.241746463715316	31.56324292853331	27.222886278730247	19.97212432902113
20	23.884421822416098	28.25896145482774	25.37457932877854	22.48203739397762
21	22.705660260849235	29.026424548233294	26.559295345380445	21.708619845537026
22	19.807163871013273	27.439452013708475	27.448935034690216	25.304449080588036
23	21.945915905452075	27.665941840411783	25.772645674639755	24.615496579496384
24	19.97499128885282	27.767829182122522	26.326409992898757	25.930769536125897
25	21.897618659056725	27.04050352636059	24.59322250695789	26.46865530762479
26	21.775221527780843	27.856043330789827	25.985021237556293	24.38371390387304
27	18.14520931012125	25.95480789163774	25.88335443121722	30.016628367023785
28	19.624340047900283	30.66698717807349	26.470199055226466	23.238473718799757
29	21.92540611588693	29.888717851456192	23.07483647302191	25.111039559634968
30	21.84116160390965	28.63938497095549	23.27839062107171	26.24106280406314
31	21.85042408951972	30.02589085263385	22.00744527414752	26.11623978369891
32	21.81976967285783	27.005217866893673	23.270892418434993	27.904120041813506
33	20.987028109438473	27.30227901253082	23.845166526259145	27.865526351771557
34	18.264960016937117	29.30297590430529	26.170712020500968	26.261352058256627
35	19.789079970536473	27.98858508916245	23.28280132850508	28.939533611795998
36	18.563785445547612	29.08773338155707	26.880174311157766	25.468306861737556
37	19.170919323750336	30.154242438944785	21.898280265171728	28.776557972133148
38	20.503394039369972	30.14586209482139	25.46764525562255	23.88309861018609
39	20.76142042422184	25.25174112675932	25.906510645242392	28.08032780377645
40	20.43414593266614	25.95546949775274	25.534908543981373	28.075476025599745
41	19.305666435839644	22.735653071396122	29.232845656114787	28.72583483664945
42	21.122877898386122	25.738242156659506	24.305864917674146	28.833015027280222
43	18.464765063668562	24.95622372872385	26.943467962826556	29.635543244781033
44	21.799039347921013	22.811737774621673	25.265193784431084	30.124029093026227
45	23.301326299725215	24.20243382836173	27.55236612400263	24.943873747910427
46	18.511077491718897	24.199787403901713	30.43873306839684	26.85040203598255
47	20.25418906938484	25.327605294613203	26.501735613375025	27.91647002262693
48	20.065851861980143	22.53121678185964	31.99416904477309	25.40876231138712
49	20.726796370869923	22.62406217333198	27.639257060439924	29.00988439535817
50	19.507456300916104	21.344957017656064	28.803242752105014	30.344343929322825
51	20.23367927981969	21.0507628318506	31.035722319502824	27.67983556882688
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2229.0
1	1789.5
2	1350.0
3	923.5
4	497.0
5	444.5
6	392.0
7	498.0
8	604.0
9	612.0
10	620.0
11	656.5
12	693.0
13	786.0
14	879.0
15	894.0
16	909.0
17	1018.0
18	1127.0
19	1156.0
20	1185.0
21	1247.5
22	1310.0
23	1628.0
24	1946.0
25	2119.0
26	2988.5
27	3685.0
28	4084.5
29	4484.0
30	5562.5
31	6641.0
32	7795.0
33	8949.0
34	8611.0
35	8273.0
36	9775.0
37	11277.0
38	12120.0
39	12963.0
40	15156.0
41	17349.0
42	19218.5
43	21088.0
44	26912.5
45	32737.0
46	37304.5
47	41872.0
48	45846.0
49	49820.0
50	47107.0
51	44394.0
52	39816.5
53	35239.0
54	30525.5
55	25812.0
56	24350.5
57	22889.0
58	22406.0
59	21923.0
60	20384.0
61	18845.0
62	16910.0
63	14975.0
64	12794.0
65	10613.0
66	9156.0
67	7699.0
68	6197.5
69	4696.0
70	4251.0
71	3806.0
72	3300.0
73	2794.0
74	2413.0
75	1572.5
76	1113.0
77	857.5
78	602.0
79	500.0
80	398.0
81	342.5
82	287.0
83	192.0
84	97.0
85	66.0
86	35.0
87	22.5
88	10.0
89	7.0
90	4.0
91	5.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.808198913000243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3384340405979	22.45703027676091
2	10.585863713538519	6.310910624781354
3	3.9480886805034525	3.5305623814383478
4	1.993651127690619	2.377085975093169
5	1.1690446084484005	1.7423557113400205
6	0.7957605504979698	1.4232113265817237
7	0.5704373589979899	1.190259718509303
8	0.4045871323169374	0.9648010974194895
9	0.3470828371765767	0.9311322824863086
>10	3.6558141821697068	26.117294784503862
>50	0.9844862018005336	20.6686262889405
>100	0.19779290635113073	8.949678264555274
>500	0.005971106606826588	1.3273450138302783
>1k	0.002985553303413294	2.009706253759454
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	4579	1.0098314668689712	TruSeq Adapter, Index 13 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1838	0.4053440131262653	No Hit
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	1384	0.3052209543888744	TruSeq Adapter, Index 13 (95% over 23bp)
CGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	1232	0.27169957789529864	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	994	0.21921215943825229	No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	916	0.20201040044812787	TruSeq Adapter, Index 13 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	872	0.19230684409472434	TruSeq Adapter, Index 13 (96% over 25bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	770	0.16981223618456165	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT	689	0.15194887107943242	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT	604	0.13320336448763018	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	603	0.1329828291159619	No Hit
ACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	518	0.11423732252415966	TruSeq Adapter, Index 13 (100% over 23bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	488	0.1076212613741118	No Hit
GCCCTGGGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	486	0.10718019063077526	No Hit
TGAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	466	0.10276948319741003	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2053537166826187E-4	0.0	0.0	1.1262741431098133	0.0
2	2.2053537166826187E-4	0.0	0.0	1.5466145615095206	0.0
3	2.2053537166826187E-4	0.0	0.0	2.0635494726999264	0.0
4	2.2053537166826187E-4	0.0	0.0	4.24662911684405	0.0
5	2.2053537166826187E-4	0.0	0.0	4.608748197123337	0.0
6	2.2053537166826187E-4	0.0	0.0	6.496751513975327	0.0
7	2.2053537166826187E-4	0.0	0.0	7.8325342601699885	0.0
8	2.2053537166826187E-4	0.0	0.0	8.943150391891356	0.0
9	2.2053537166826187E-4	0.0	0.0	10.388318682433475	0.0
10	2.2053537166826187E-4	0.0	0.0	12.70195526660521	0.0
11	2.2053537166826187E-4	0.0	0.0	14.933773227888022	0.0
12	2.2053537166826187E-4	0.0	0.0	15.868622668389783	0.0
13	2.2053537166826187E-4	0.0	0.0	16.390850428500226	0.0
14	2.2053537166826187E-4	0.0	0.0	16.894994288133873	0.0
15	2.2053537166826187E-4	0.0	0.0	17.351723042858843	0.0
16	2.2053537166826187E-4	0.0	0.0	18.221955619461806	0.0
17	2.2053537166826187E-4	0.0	0.0	19.156805059963567	0.0
18	2.2053537166826187E-4	0.0	0.0	20.605942987195718	0.0
19	2.2053537166826187E-4	0.0	0.0	21.137653768287894	0.0
20	2.2053537166826187E-4	0.0	0.0	21.968410513362237	0.0
21	2.2053537166826187E-4	0.0	0.0	22.63663268951707	0.0
22	2.2053537166826187E-4	0.0	0.0	23.269348670833313	0.0
23	2.2053537166826187E-4	0.0	0.0	23.904931611981247	0.0
24	2.2053537166826187E-4	0.0	0.0	24.353721093326158	0.0
25	2.2053537166826187E-4	0.0	0.0	24.73017497276388	0.0
26	2.2053537166826187E-4	0.0	0.0	25.0830315674331	0.0
27	2.2053537166826187E-4	0.0	0.0	25.540642463644744	0.0
28	2.2053537166826187E-4	0.0	0.0	25.959218599071104	0.0
29	2.2053537166826187E-4	0.0	0.0	26.36985546111741	0.0
30	2.2053537166826187E-4	0.0	0.0	26.969491136683413	0.0
31	2.2053537166826187E-4	0.0	0.0	27.362264633624587	0.0
32	2.2053537166826187E-4	0.0	0.0	27.777532738475923	0.0
33	2.2053537166826187E-4	0.0	0.0	28.191036560353915	0.0
34	4.4107074333652374E-4	0.0	0.0	28.54698065022649	0.0
35	4.4107074333652374E-4	0.0	0.0	28.93644611659264	0.0
36	4.4107074333652374E-4	0.0	0.0	29.302755368933624	0.0
37	4.4107074333652374E-4	0.0	0.0	29.67082890424795	0.0
38	4.4107074333652374E-4	0.0	0.0	30.113443395186152	0.0
39	4.4107074333652374E-4	0.0	0.0	30.490338345367213	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	30	2.1625838E-6	45.000004	1
TAGCGAG	30	2.1625838E-6	45.000004	1
TTCACGG	45	3.8380676E-10	45.000004	2
GCGCCTA	35	1.2097553E-7	45.000004	25
CGCGACG	30	2.1625838E-6	45.000004	1
ATACTAA	30	2.1625838E-6	45.000004	38
TGCATAG	35	1.2097553E-7	45.000004	1
CGCCTAG	35	1.2097553E-7	45.000004	26
AAACGCG	30	2.1625838E-6	45.000004	1
ACGATGA	30	2.1625838E-6	45.000004	30
CCACTAG	35	1.2097553E-7	45.000004	1
TAACACG	35	1.2097553E-7	45.000004	1
TATTACG	30	2.1625838E-6	45.000004	1
TAGGGTA	70	0.0	45.000004	5
CATACCG	35	1.2097553E-7	45.000004	1
GGGCCTA	30	2.1625838E-6	45.000004	7
GATTGGT	35	1.2097553E-7	45.000004	9
GCGTGGC	30	2.1625838E-6	45.000004	44
TCATACG	45	3.8380676E-10	45.000004	1
TATACGG	70	0.0	45.000004	2
>>END_MODULE