FastQCFastQC Report
Sat 18 Jun 2016
SRR3548467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463221
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT48421.0452893975014086TruSeq Adapter, Index 13 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17710.38232290850371636No Hit
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT14940.32252423789076917TruSeq Adapter, Index 13 (95% over 23bp)
CGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC13450.2903581659726135No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT11030.23811528406527338No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT9440.2037904153740871TruSeq Adapter, Index 13 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC9330.20141573892375345TruSeq Adapter, Index 13 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT8880.19170115344511585No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC8200.17702133538850787No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT6150.1327660015413809No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG5560.12002910058050045No Hit
GCCCTGGGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG5510.11894970219398517No Hit
ACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG5250.11333683058410565TruSeq Adapter, Index 13 (100% over 23bp)
GCCCTGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTC5170.11160979316568118No Hit
GCCCTGGGGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTC4890.10556516220119555No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.10340636542816495No Hit
TACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT4710.10167932800974049TruSeq Adapter, Index 13 (95% over 23bp)
TGAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT4680.10103168897783131No Hit
AACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT4680.10103168897783131TruSeq Adapter, Index 13 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG302.1626856E-645.0000042
GAATACG302.1626856E-645.0000041
AGTGCGC302.1626856E-645.00000413
TAGATCG302.1626856E-645.00000413
ACGTAAG302.1626856E-645.0000041
AACTAAG351.209828E-745.0000041
ATACACG351.209828E-745.0000041
ATTTACG207.028921E-445.01
AGGTATG207.028921E-445.040
TTCTACG253.887188E-545.01
CGGGTAC551.8189894E-1245.06
TCGTTGA253.887188E-545.024
GATCGCT207.028921E-445.015
CGAAACG253.887188E-545.01
AGCCCGT207.028921E-445.014
CTTCACG253.887188E-545.01
CTATATG207.028921E-445.01
CGTTGGA207.028921E-445.010
CGACCGT207.028921E-445.033
CGATTCG207.028921E-445.028
Produced by FastQC (version 0.11.5)