##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548467_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463221
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.617912400344544	31.0	31.0	34.0	30.0	34.0
2	31.827725858715386	33.0	31.0	34.0	30.0	34.0
3	31.988996612847863	33.0	31.0	34.0	30.0	34.0
4	35.691220821163114	37.0	35.0	37.0	33.0	37.0
5	35.477495623039545	37.0	35.0	37.0	33.0	37.0
6	35.49996869744679	37.0	35.0	37.0	33.0	37.0
7	35.56332290634492	37.0	35.0	37.0	33.0	37.0
8	35.634990641615985	37.0	35.0	37.0	35.0	37.0
9	37.2787848564724	39.0	37.0	39.0	34.0	39.0
10	36.860748972952436	39.0	37.0	39.0	32.0	39.0
11	36.879120333490924	39.0	37.0	39.0	33.0	39.0
12	36.76887705868257	39.0	35.0	39.0	33.0	39.0
13	36.716288769291545	39.0	35.0	39.0	33.0	39.0
14	37.79683995328364	40.0	37.0	41.0	33.0	41.0
15	37.79972626456918	40.0	37.0	41.0	33.0	41.0
16	37.7404586579624	40.0	36.0	41.0	33.0	41.0
17	37.70846313098931	39.0	36.0	41.0	33.0	41.0
18	37.63145453250176	39.0	36.0	41.0	33.0	41.0
19	37.624503206892605	39.0	36.0	41.0	32.0	41.0
20	37.537713100226455	39.0	36.0	41.0	32.0	41.0
21	37.49473145647542	39.0	36.0	41.0	32.0	41.0
22	37.489114267271994	39.0	36.0	41.0	32.0	41.0
23	37.3556444979826	39.0	36.0	41.0	32.0	41.0
24	37.25081548548101	39.0	35.0	41.0	32.0	41.0
25	37.28955941116659	39.0	35.0	41.0	32.0	41.0
26	37.227118373303455	39.0	35.0	41.0	32.0	41.0
27	37.171194742898095	39.0	35.0	41.0	32.0	41.0
28	37.0196752737894	39.0	35.0	41.0	31.0	41.0
29	36.84388445256152	39.0	35.0	40.0	31.0	41.0
30	36.828673570498744	39.0	35.0	40.0	31.0	41.0
31	36.75645318325378	39.0	35.0	40.0	31.0	41.0
32	36.67796796777348	39.0	35.0	40.0	30.0	41.0
33	36.60415654730679	39.0	35.0	40.0	30.0	41.0
34	36.50649042249812	39.0	35.0	40.0	30.0	41.0
35	36.46584243805872	39.0	35.0	40.0	30.0	41.0
36	36.34080708776157	39.0	35.0	40.0	30.0	41.0
37	36.35645404677249	39.0	35.0	40.0	30.0	41.0
38	36.28709406525179	39.0	35.0	40.0	30.0	41.0
39	36.25787043333528	39.0	35.0	40.0	30.0	41.0
40	36.21345534852694	38.0	35.0	40.0	30.0	41.0
41	36.17743366557216	38.0	35.0	40.0	30.0	41.0
42	36.07881119379303	38.0	35.0	40.0	30.0	41.0
43	35.97165283957334	38.0	35.0	40.0	30.0	41.0
44	35.86986341292817	38.0	35.0	40.0	29.0	41.0
45	35.66585064148646	38.0	35.0	40.0	28.0	41.0
46	35.52480997191405	38.0	34.0	40.0	28.0	41.0
47	35.44012037450806	38.0	34.0	40.0	28.0	41.0
48	35.360728464383094	38.0	34.0	40.0	27.0	41.0
49	35.234669844415514	38.0	34.0	40.0	27.0	41.0
50	35.16564015880109	37.0	34.0	40.0	27.0	41.0
51	33.856295375209676	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	4.0
12	1.0
13	4.0
14	16.0
15	21.0
16	36.0
17	90.0
18	194.0
19	406.0
20	735.0
21	1126.0
22	1540.0
23	1974.0
24	2498.0
25	2938.0
26	3542.0
27	4302.0
28	5537.0
29	6992.0
30	9049.0
31	11893.0
32	15525.0
33	21179.0
34	32661.0
35	40029.0
36	44576.0
37	63615.0
38	92769.0
39	99941.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.510512260886273	31.670628058745177	19.9546220918309	27.864237588537655
2	24.46240563359606	30.04678112607157	19.9054015254058	25.585411714926572
3	20.486765496382937	32.67511619723631	19.742196489364687	27.095921817016066
4	19.263159485429203	32.618555721782904	19.719313243570564	28.39897154921733
5	17.594841339231166	33.3445590765531	20.4770509109043	28.583548673311444
6	21.928841740767364	36.17517340535079	19.69016948713465	22.20581536674719
7	79.22848920925432	7.688338827471121	7.219879927723484	5.863292035551066
8	80.87543526739937	5.154774934642427	7.124029351000926	6.845760446957285
9	73.98801004272258	9.61312203030519	10.922432273148239	5.476435653823985
10	32.74959468590586	33.82705015532543	13.462040796941416	19.96131436182729
11	25.945499016668073	27.721757001517634	27.68959092959948	18.643153052214817
12	26.93314854032956	21.088853916381165	33.09910388345951	18.878893659829757
13	27.400743921367987	22.226539815768284	27.78652090470855	22.586195358155177
14	20.706746887554754	26.765410031065084	31.322845898609952	21.20499718277021
15	18.150947387963846	29.6890685007804	30.535316835808395	21.624667275447358
16	20.507058186049424	25.923911048937764	29.72835860204956	23.84067216296325
17	19.158457841937217	26.900766588734104	29.135337128498062	24.805438440830617
18	21.852852094356688	25.303472856368774	28.051405268759407	24.792269780515134
19	20.941408096783178	31.917810289257183	27.304029825936215	19.836751788023427
20	23.848227951668857	28.280453606377947	25.536407028178775	22.33491141377442
21	22.323469790877358	29.158436253969487	26.671286491760952	21.846807463392203
22	19.59604594783052	27.38347354718374	27.533725802586673	25.48675470239907
23	21.902936179490997	27.675774630252082	25.85504543187809	24.566243758378832
24	19.63641544748619	28.00693405523497	26.377690130628796	25.978960366650046
25	21.761534990857495	26.94113608838978	24.676774153157996	26.620554767594733
26	21.809028519864167	27.909572320771296	26.062937561120936	24.218461598243604
27	17.92686428292327	25.814675932222414	25.886132105409732	30.372327679444584
28	19.406719470835736	30.69182960185311	26.57522003536109	23.32623089195006
29	21.800393332772046	29.834139643928058	22.97456289762338	25.39090412567651
30	21.7759989292368	28.54943968429756	23.189147296862618	26.485414089603022
31	21.62984838770263	30.268921314016417	21.825003615984595	26.276226682296354
32	21.635677138989813	27.142767706990835	23.14381256462898	28.077742589390375
33	20.73977647818212	27.240129441454513	23.75302501397821	28.267069066385158
34	18.191316887619514	29.351000926123817	26.072652146599573	26.3850300396571
35	19.543587186245873	28.028306143287978	23.100636629168367	29.327470041297783
36	18.421228743947275	28.98508487309513	26.997696563843178	25.59598981911442
37	18.894221116918274	30.320516556891853	21.76822726085389	29.017035065335982
38	20.42437626964235	30.195738103410687	25.45782682564046	23.922058801306502
39	20.70070225659027	25.102920636154234	25.978528607295438	28.217848499960063
40	20.3006772145477	25.8837574289594	25.564687265905473	28.250878090587427
41	19.19753206352907	22.568277344939027	29.415980708992034	28.81820988253987
42	21.202406626642574	25.52690832237744	24.44340822199339	28.827276828986598
43	18.39208498751136	24.809971914053982	27.074765608640366	29.72317748979429
44	21.818743105342804	22.629803052970395	25.39543759889988	30.15601624278692
45	23.429205498023624	24.139449636350683	27.42470656554862	25.006638300077068
46	18.439146757163428	24.00430895835897	30.74601540085618	26.810528883621426
47	20.342989631299098	25.14458541387372	26.360203876767248	28.15222107805993
48	20.043348639202456	22.330377940551056	32.19305687781858	25.43321654242791
49	20.690771791434326	22.403129391802185	27.750900757953545	29.15519805880994
50	19.535815517862964	21.139369760870082	28.713076479693278	30.611738241573676
51	20.070333598865336	20.963643703545394	31.233903471561092	27.73211922602818
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2172.0
1	1770.5
2	1369.0
3	934.5
4	500.0
5	461.0
6	422.0
7	532.0
8	642.0
9	636.0
10	630.0
11	681.5
12	733.0
13	810.0
14	887.0
15	903.5
16	920.0
17	1046.5
18	1173.0
19	1223.5
20	1274.0
21	1394.0
22	1514.0
23	1752.5
24	1991.0
25	2122.5
26	3023.0
27	3792.0
28	4045.5
29	4299.0
30	5528.0
31	6757.0
32	7937.0
33	9117.0
34	8687.0
35	8257.0
36	9762.5
37	11268.0
38	12205.5
39	13143.0
40	15367.0
41	17591.0
42	19556.5
43	21522.0
44	27567.0
45	33612.0
46	38655.0
47	43698.0
48	47942.5
49	52187.0
50	48960.5
51	45734.0
52	41208.0
53	36682.0
54	31341.5
55	26001.0
56	24534.0
57	23067.0
58	22426.0
59	21785.0
60	20459.0
61	19133.0
62	17044.0
63	14955.0
64	12851.5
65	10748.0
66	9154.5
67	7561.0
68	6136.0
69	4711.0
70	4229.5
71	3748.0
72	3257.5
73	2767.0
74	2383.0
75	1545.5
76	1092.0
77	864.5
78	637.0
79	518.5
80	400.0
81	345.5
82	291.0
83	199.5
84	108.0
85	74.0
86	40.0
87	28.0
88	16.0
89	12.0
90	8.0
91	8.5
92	9.0
93	6.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	463221.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.40184438316647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15245690489876	22.096208429304575
2	10.665545061380246	6.271733923127034
3	4.0114946476696485	3.5383602412406465
4	1.9774969210727287	2.3256822696628476
5	1.1362579959263037	1.6704040387676895
6	0.7789462100235517	1.3741473149981862
7	0.5639343990458248	1.160649801014189
8	0.4477679894655716	1.0532163796824046
9	0.3465073835056947	0.9169160550707359
>10	3.697637823394055	25.819537850061096
>50	0.9966244505563425	20.55960568125837
>100	0.2149871868879879	9.541698877555588
>500	0.006649088254267667	1.3791118486033744
>1k	0.003693937919037592	2.2927272896532713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGCT	4842	1.0452893975014086	TruSeq Adapter, Index 13 (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1771	0.38232290850371636	No Hit
AAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	1494	0.32252423789076917	TruSeq Adapter, Index 13 (95% over 23bp)
CGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	1345	0.2903581659726135	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	1103	0.23811528406527338	No Hit
AATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	944	0.2037904153740871	TruSeq Adapter, Index 13 (100% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC	933	0.20141573892375345	TruSeq Adapter, Index 13 (96% over 25bp)
AATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGT	888	0.19170115344511585	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCC	820	0.17702133538850787	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT	615	0.1327660015413809	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	556	0.12002910058050045	No Hit
GCCCTGGGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	551	0.11894970219398517	No Hit
ACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTG	525	0.11333683058410565	TruSeq Adapter, Index 13 (100% over 23bp)
GCCCTGGGGAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTC	517	0.11160979316568118	No Hit
GCCCTGGGGAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTC	489	0.10556516220119555	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.10340636542816495	No Hit
TACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	471	0.10167932800974049	TruSeq Adapter, Index 13 (95% over 23bp)
TGAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTT	468	0.10103168897783131	No Hit
AACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT	468	0.10103168897783131	TruSeq Adapter, Index 13 (100% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1860429471030027	0.0
2	0.0	0.0	0.0	1.6450031410493047	0.0
3	0.0	0.0	0.0	2.163762005608554	0.0
4	0.0	0.0	0.0	4.54081313239253	0.0
5	0.0	0.0	0.0	4.929180671860732	0.0
6	0.0	0.0	0.0	6.913114906275838	0.0
7	0.0	0.0	0.0	8.31093581681314	0.0
8	0.0	0.0	0.0	9.480356028763808	0.0
9	0.0	0.0	0.0	11.016771692129675	0.0
10	0.0	0.0	0.0	13.445202182111778	0.0
11	0.0	0.0	0.0	15.746263662485077	0.0
12	0.0	0.0	0.0	16.72851619421399	0.0
13	0.0	0.0	0.0	17.301029098421704	0.0
14	0.0	0.0	0.0	17.82755963136386	0.0
15	0.0	0.0	0.0	18.29040565950162	0.0
16	0.0	0.0	0.0	19.188680996759647	0.0
17	0.0	0.0	0.0	20.13250694592862	0.0
18	0.0	0.0	0.0	21.638699454472057	0.0
19	0.0	0.0	0.0	22.15983299548164	2.1587967730305838E-4
20	0.0	0.0	0.0	23.044723792746876	2.1587967730305838E-4
21	0.0	0.0	0.0	23.713519033031748	2.1587967730305838E-4
22	0.0	0.0	0.0	24.349716442043864	2.1587967730305838E-4
23	0.0	0.0	0.0	25.004263623626734	2.1587967730305838E-4
24	0.0	0.0	0.0	25.45696330693125	2.1587967730305838E-4
25	0.0	0.0	0.0	25.848353161881693	2.1587967730305838E-4
26	0.0	0.0	0.0	26.197646479758042	2.1587967730305838E-4
27	0.0	0.0	0.0	26.650130283385252	2.1587967730305838E-4
28	0.0	0.0	0.0	27.08318491605519	2.1587967730305838E-4
29	0.0	0.0	0.0	27.47802884584248	2.1587967730305838E-4
30	0.0	0.0	0.0	28.069539161652862	2.1587967730305838E-4
31	0.0	0.0	0.0	28.454452626284215	2.1587967730305838E-4
32	0.0	0.0	0.0	28.87261156122024	2.1587967730305838E-4
33	0.0	0.0	0.0	29.295951608411535	2.1587967730305838E-4
34	0.0	0.0	0.0	29.659708864667188	2.1587967730305838E-4
35	0.0	0.0	0.0	30.059518027032453	2.1587967730305838E-4
36	0.0	0.0	0.0	30.45047612262829	2.1587967730305838E-4
37	0.0	0.0	0.0	30.80322351534149	2.1587967730305838E-4
38	0.0	0.0	0.0	31.23519874962491	2.1587967730305838E-4
39	0.0	0.0	0.0	31.61989633457896	2.1587967730305838E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	30	2.1626856E-6	45.000004	2
GAATACG	30	2.1626856E-6	45.000004	1
AGTGCGC	30	2.1626856E-6	45.000004	13
TAGATCG	30	2.1626856E-6	45.000004	13
ACGTAAG	30	2.1626856E-6	45.000004	1
AACTAAG	35	1.209828E-7	45.000004	1
ATACACG	35	1.209828E-7	45.000004	1
ATTTACG	20	7.028921E-4	45.0	1
AGGTATG	20	7.028921E-4	45.0	40
TTCTACG	25	3.887188E-5	45.0	1
CGGGTAC	55	1.8189894E-12	45.0	6
TCGTTGA	25	3.887188E-5	45.0	24
GATCGCT	20	7.028921E-4	45.0	15
CGAAACG	25	3.887188E-5	45.0	1
AGCCCGT	20	7.028921E-4	45.0	14
CTTCACG	25	3.887188E-5	45.0	1
CTATATG	20	7.028921E-4	45.0	1
CGTTGGA	20	7.028921E-4	45.0	10
CGACCGT	20	7.028921E-4	45.0	33
CGATTCG	20	7.028921E-4	45.0	28
>>END_MODULE