SRR3548466_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233798
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	3649	1.5607490226605873	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2364	1.0111292654342638	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	1452	0.6210489396829743	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	1093	0.46749758338394687	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	735	0.3143739467403485	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	630	0.2694633829202987	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	581	0.24850511980427548	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	514	0.2198479028905294	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	503	0.21514298668080992	No Hit
AGCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	460	0.19675104149736097	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	456	0.19504016287564477	No Hit
AGCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	424	0.18135313390191532	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC	422	0.18049769459105725	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT	404	0.1727987407933344	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	381	0.16296118871846635	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	380	0.1625334690630373	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	366	0.15654539388703068	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	343	0.14670784181216265	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	317	0.13558713077100745	No Hit
AGCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	314	0.1343039718047203	No Hit
GCCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	296	0.1266050180069975	No Hit
GCTCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	293	0.12532185904071036	No Hit
CATGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTA	284	0.12147238214184894	No Hit
TACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	254	0.10864079247897758	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	249	0.10650219420183235	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	246	0.10521903523554521	No Hit
GCTTATGGGATTATTTTGAGACTGGATTTTGGTGAAAAACAATTCATCCGG	245	0.10479131558011616	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	234	0.10008639937039666	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTT	30	2.15806E-6	45.000004	6
GTCATCT	20	7.021745E-4	45.000004	37
CAGAATC	30	2.15806E-6	45.000004	25
AACAATT	40	6.7757355E-9	45.000004	38
ACTATTA	20	7.021745E-4	45.000004	19
ATGGGCC	20	7.021745E-4	45.000004	5
AAGTAGG	30	2.15806E-6	45.000004	2
ACTATGG	20	7.021745E-4	45.000004	2
CAGCGCG	30	2.15806E-6	45.000004	1
AGCAAAA	20	7.021745E-4	45.000004	40
TTGGCGG	20	7.021745E-4	45.000004	35
ATCTCCC	30	2.15806E-6	45.000004	10
AGATCGC	20	7.021745E-4	45.000004	21
GATAATA	30	2.15806E-6	45.000004	9
ACGCCCA	30	2.15806E-6	45.000004	35
TCACCGT	20	7.021745E-4	45.000004	17
AGTCATC	20	7.021745E-4	45.000004	36
ATTAGCG	20	7.021745E-4	45.000004	1
CGAGTTG	20	7.021745E-4	45.000004	20
CAAGTAC	20	7.021745E-4	45.000004	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content