##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233798
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.43023892419952	31.0	31.0	34.0	30.0	34.0
2	31.629321037818972	31.0	31.0	34.0	30.0	34.0
3	31.856910666472768	33.0	31.0	34.0	30.0	34.0
4	35.57379019495462	37.0	35.0	37.0	33.0	37.0
5	35.34425444186862	37.0	35.0	37.0	33.0	37.0
6	34.68261490688543	37.0	35.0	37.0	32.0	37.0
7	35.11726362073243	37.0	35.0	37.0	32.0	37.0
8	35.49446103046219	37.0	35.0	37.0	33.0	37.0
9	36.97545744617148	39.0	37.0	39.0	33.0	39.0
10	36.637037100402914	38.0	35.0	39.0	32.0	39.0
11	36.75871051078281	39.0	36.0	39.0	32.0	39.0
12	36.84883531937827	39.0	37.0	39.0	33.0	39.0
13	36.79246614598927	39.0	37.0	39.0	33.0	39.0
14	37.86666267461655	39.0	37.0	41.0	33.0	41.0
15	37.90345084218	40.0	37.0	41.0	33.0	41.0
16	37.75280797953789	39.0	37.0	41.0	33.0	41.0
17	37.73429199565437	39.0	37.0	41.0	33.0	41.0
18	37.65878236768492	39.0	37.0	41.0	33.0	41.0
19	37.57935482767175	39.0	36.0	41.0	32.0	41.0
20	37.471702067596816	39.0	36.0	40.0	32.0	41.0
21	37.39927202114646	39.0	36.0	40.0	32.0	41.0
22	37.345674471124646	39.0	36.0	40.0	32.0	41.0
23	37.20712752033807	39.0	36.0	40.0	32.0	41.0
24	37.107267812385054	39.0	35.0	40.0	31.0	41.0
25	37.05969255511168	39.0	35.0	40.0	31.0	41.0
26	37.09161327299635	39.0	36.0	40.0	31.0	41.0
27	37.01219001017973	39.0	35.0	40.0	31.0	41.0
28	36.76601168530099	39.0	35.0	40.0	31.0	41.0
29	36.4982591810024	39.0	35.0	40.0	30.0	41.0
30	36.54873437753959	39.0	35.0	40.0	30.0	41.0
31	36.47807509046271	39.0	35.0	40.0	30.0	41.0
32	36.493284801409764	39.0	35.0	40.0	30.0	41.0
33	36.195728791520885	39.0	35.0	40.0	29.0	41.0
34	36.32371534401492	39.0	35.0	40.0	30.0	41.0
35	36.22723034414324	39.0	35.0	40.0	30.0	41.0
36	36.18546779698714	39.0	35.0	40.0	29.0	41.0
37	36.07331114894054	39.0	35.0	40.0	29.0	41.0
38	35.91940478532751	39.0	35.0	40.0	28.0	41.0
39	35.85548636002019	39.0	35.0	40.0	28.0	41.0
40	35.81934404913643	39.0	35.0	40.0	28.0	41.0
41	35.85663264869674	39.0	35.0	40.0	28.0	41.0
42	35.73238864318771	39.0	35.0	40.0	27.0	41.0
43	35.671545522202926	38.0	35.0	40.0	27.0	41.0
44	35.58856363185314	38.0	35.0	40.0	27.0	41.0
45	35.399678354819116	38.0	35.0	40.0	26.0	41.0
46	35.22572049375957	38.0	34.0	40.0	26.0	41.0
47	35.12807209642512	38.0	34.0	40.0	26.0	41.0
48	35.108456873027144	38.0	34.0	40.0	26.0	41.0
49	34.95766003130908	38.0	34.0	40.0	24.0	41.0
50	34.87681673923643	38.0	34.0	40.0	24.0	41.0
51	33.58581767166528	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	5.0
15	7.0
16	32.0
17	83.0
18	130.0
19	325.0
20	560.0
21	963.0
22	1330.0
23	1643.0
24	1843.0
25	1953.0
26	2164.0
27	2448.0
28	3006.0
29	3670.0
30	4745.0
31	6031.0
32	7926.0
33	10234.0
34	14144.0
35	19258.0
36	22867.0
37	32291.0
38	48078.0
39	48051.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.4394391739878	33.87710758860213	19.12206263526634	26.56139060214373
2	19.648585531099496	34.82750066296546	20.110950478618296	25.412963327316746
3	19.96980299232671	33.012686164980025	20.729433100368695	26.28807774232457
4	17.303826380037467	32.480175193970865	19.48091942617131	30.73507899982036
5	15.16009546702709	36.37841213355119	17.735395512365375	30.726096887056347
6	18.639594863942378	39.56663444511929	18.21786328368934	23.57590740724899
7	76.33042198821205	8.54113379926261	8.799048751486325	6.329395461039017
8	79.06098426847107	7.601005996629569	6.369173388993918	6.96883634590544
9	69.8222397112037	13.178042583768896	12.325597310498807	4.67412039452861
10	23.360764420568184	46.40416085680801	14.001830640125238	16.233244082498565
11	15.735806123234585	32.59352090265956	35.14401320798296	16.526659766122894
12	19.688791178709828	23.21106254116802	38.20691366051035	18.8932326196118
13	25.492946902881975	26.07592879323176	28.951060317025807	19.48006398686045
14	16.153688226588763	32.52080856123663	33.046903737414354	18.278599474760263
15	14.24220908647636	33.00798124877031	33.582836465666944	19.16697319908639
16	16.309378181164938	34.375828706832394	27.59219497172773	21.722598140274936
17	14.769587421620372	31.087519995893892	28.915987305280627	25.22690527720511
18	19.73669578011788	27.616147272431757	26.649073131506682	25.99808381594368
19	18.783308668166537	33.77702118923173	28.802641596591933	18.637028546009805
20	18.720861598473896	30.589654316974485	27.340695814335454	23.34878827021617
21	19.586993900717715	31.23166151977348	27.4061369216161	21.77520765789271
22	15.73067348736944	31.64098923001908	27.84369412912001	24.784643153491476
23	18.425307316572425	28.9685968228984	26.350097092361786	26.255998768167395
24	14.883788569619929	32.709860648936264	25.966432561441927	26.439918220001886
25	16.96293381466052	32.42713795669766	27.40357060368352	23.206357624958297
26	19.542083336897665	29.95534606797321	25.776097314776003	24.726473280353126
27	15.29097768158838	28.43095321602409	27.684154697644974	28.593914404742556
28	19.38254390542263	27.469011710964164	31.660664334168814	21.487780049444392
29	19.492895576523324	28.821033541775375	25.287641468276036	26.398429413425266
30	18.554478652511996	29.21881282132439	29.4339558080052	22.79275271815841
31	20.990342090180413	29.81163226374905	23.273509610860657	25.924516035209884
32	19.94798928989983	25.669595120574172	26.57037271490774	27.81204287461826
33	18.577147794249736	30.10590338668423	28.49810520192645	22.81884361713958
34	17.8491689407095	24.162738774497644	28.44592340396411	29.54216888082875
35	17.408617695617583	26.352663410294358	29.79708979546446	26.441629098623597
36	18.13616882950239	24.892428506659595	33.312089923780356	23.659312740057654
37	20.317539072190524	26.672597712555284	27.55626652067169	25.453596694582505
38	20.563477874062226	26.56695095766431	29.200420876140942	23.669150292132525
39	19.52155279343707	25.277803916201165	27.684154697644974	27.516488592716794
40	20.74440328830871	26.780810785378833	26.531450226263697	25.94333570004876
41	18.01041925080625	22.12037741982395	32.27786379695293	27.591339532416875
42	22.28547720681956	24.707225895858816	29.8830614462057	23.124235451115922
43	18.272611399584257	23.323125090890425	30.62985996458481	27.77440354494051
44	22.144757440183408	20.795729646960197	29.358677148649686	27.700835764206712
45	23.418506574051104	23.42962728509226	28.414699869117786	24.73716627173885
46	17.541210788800587	24.42065372672136	32.14569842342535	25.892437061052703
47	19.533101224133652	24.903121498045323	27.066099795550002	28.497677482271023
48	18.547207418369705	20.83422441594881	34.01397787833942	26.604590287342067
49	20.594701408908545	21.477942497369522	31.89291610706678	26.034439986655144
50	19.240968699475616	21.46168915046322	28.504520996757886	30.79282115330328
51	19.415050599235236	20.09512485136742	34.177366786713314	26.312457762684026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2723.0
1	2190.0
2	1657.0
3	1105.5
4	554.0
5	497.5
6	441.0
7	526.0
8	611.0
9	634.0
10	657.0
11	720.5
12	784.0
13	860.5
14	937.0
15	994.0
16	1051.0
17	1036.0
18	1021.0
19	1056.5
20	1092.0
21	1276.5
22	1461.0
23	1549.5
24	1638.0
25	1718.0
26	2081.0
27	2364.0
28	2577.0
29	2790.0
30	3472.0
31	4154.0
32	4077.5
33	4001.0
34	4225.0
35	4449.0
36	5442.5
37	6436.0
38	6873.0
39	7310.0
40	8052.5
41	8795.0
42	11172.0
43	13549.0
44	16976.5
45	20404.0
46	22313.5
47	24223.0
48	27682.0
49	31141.0
50	28267.0
51	25393.0
52	21823.0
53	18253.0
54	15686.5
55	13120.0
56	11132.0
57	9144.0
58	8165.5
59	7187.0
60	6267.0
61	5347.0
62	4264.5
63	3182.0
64	2549.0
65	1916.0
66	1809.0
67	1702.0
68	1321.0
69	940.0
70	824.0
71	708.0
72	554.5
73	401.0
74	303.5
75	121.0
76	36.0
77	76.5
78	117.0
79	86.5
80	56.0
81	33.0
82	10.0
83	20.0
84	30.0
85	18.0
86	6.0
87	3.0
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	233798.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.21631493853669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24080863339118	28.746182602075294
2	10.499821861603706	7.8152935439995215
3	3.7891760811851376	4.230575111848689
4	1.8560871613933871	2.7630689740716345
5	1.0883681373619427	2.025252568456531
6	0.683821585776511	1.5269591698816927
7	0.5229223891232143	1.3622871025415102
8	0.3942030318005769	1.1736627344973012
9	0.30685775361735873	1.0278103319959966
>10	2.9973221776556986	24.351363142541853
>50	0.49993678960131477	12.701990607276366
>100	0.11033087770511774	7.347795960615574
>500	0.005746399880474882	1.267333339036262
>1k	0.004597119904379906	3.6604248111617723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	3649	1.5607490226605873	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2364	1.0111292654342638	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	1452	0.6210489396829743	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	1093	0.46749758338394687	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	735	0.3143739467403485	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	630	0.2694633829202987	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	581	0.24850511980427548	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	514	0.2198479028905294	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	503	0.21514298668080992	No Hit
AGCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	460	0.19675104149736097	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	456	0.19504016287564477	No Hit
AGCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	424	0.18135313390191532	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC	422	0.18049769459105725	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT	404	0.1727987407933344	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	381	0.16296118871846635	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	380	0.1625334690630373	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	366	0.15654539388703068	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	343	0.14670784181216265	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	317	0.13558713077100745	No Hit
AGCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	314	0.1343039718047203	No Hit
GCCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	296	0.1266050180069975	No Hit
GCTCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	293	0.12532185904071036	No Hit
CATGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTA	284	0.12147238214184894	No Hit
TACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	254	0.10864079247897758	No Hit
GCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	249	0.10650219420183235	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	246	0.10521903523554521	No Hit
GCTTATGGGATTATTTTGAGACTGGATTTTGGTGAAAAACAATTCATCCGG	245	0.10479131558011616	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	234	0.10008639937039666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6950529944653077	0.0
2	0.0	0.0	0.0	2.410627977998101	0.0
3	0.0	0.0	0.0	3.188650031223535	0.0
4	0.0	0.0	0.0	6.615539910521048	0.0
5	0.0	0.0	0.0	7.168581424990804	0.0
6	0.0	0.0	0.0	9.618987330943806	0.0
7	0.0	0.0	0.0	11.574093875909973	0.0
8	0.0	0.0	0.0	13.00053892676584	0.0
9	0.0	0.0	0.0	14.904319113080522	0.0
10	0.0	0.0	0.0	17.888519149008975	0.0
11	0.0	0.0	0.0	20.30513520218308	0.0
12	0.0	0.0	0.0	21.714043747166357	0.0
13	0.0	0.0	0.0	22.41593170172542	0.0
14	0.0	0.0	0.0	22.95870794446488	0.0
15	0.0	0.0	0.0	23.588738996911864	0.0
16	0.0	0.0	0.0	24.704659577926243	0.0
17	0.0	0.0	0.0	26.133670946714684	0.0
18	0.0	0.0	0.0	28.649090240292903	0.0
19	0.0	0.0	0.0	29.3543999520954	0.0
20	0.0	0.0	0.0	30.575967288000754	0.0
21	0.0	0.0	0.0	31.336452835353597	0.0
22	0.0	0.0	0.0	32.23423639209916	0.0
23	0.0	0.0	0.0	33.08710938502468	0.0
24	0.0	0.0	0.0	33.60251156981668	0.0
25	0.0	0.0	0.0	34.084123901829784	0.0
26	0.0	0.0	0.0	34.51056039829254	0.0
27	0.0	0.0	0.0	35.25693119701623	0.0
28	0.0	0.0	0.0	35.84290712495402	0.0
29	0.0	0.0	0.0	36.34376684146143	0.0
30	0.0	0.0	0.0	37.202627909562956	0.0
31	0.0	0.0	0.0	37.67910760571091	0.0
32	0.0	0.0	0.0	38.14403887116229	0.0
33	0.0	0.0	0.0	38.59442766832907	0.0
34	0.0	0.0	0.0	39.04909366205015	0.0
35	0.0	0.0	0.0	39.559790930632424	0.0
36	0.0	0.0	0.0	40.03284886953695	0.0
37	0.0	0.0	0.0	40.51617208017177	0.0
38	0.0	0.0	0.0	40.969127195271135	0.0
39	0.0	0.0	0.0	41.408395281396764	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTT	30	2.15806E-6	45.000004	6
GTCATCT	20	7.021745E-4	45.000004	37
CAGAATC	30	2.15806E-6	45.000004	25
AACAATT	40	6.7757355E-9	45.000004	38
ACTATTA	20	7.021745E-4	45.000004	19
ATGGGCC	20	7.021745E-4	45.000004	5
AAGTAGG	30	2.15806E-6	45.000004	2
ACTATGG	20	7.021745E-4	45.000004	2
CAGCGCG	30	2.15806E-6	45.000004	1
AGCAAAA	20	7.021745E-4	45.000004	40
TTGGCGG	20	7.021745E-4	45.000004	35
ATCTCCC	30	2.15806E-6	45.000004	10
AGATCGC	20	7.021745E-4	45.000004	21
GATAATA	30	2.15806E-6	45.000004	9
ACGCCCA	30	2.15806E-6	45.000004	35
TCACCGT	20	7.021745E-4	45.000004	17
AGTCATC	20	7.021745E-4	45.000004	36
ATTAGCG	20	7.021745E-4	45.000004	1
CGAGTTG	20	7.021745E-4	45.000004	20
CAAGTAC	20	7.021745E-4	45.000004	30
>>END_MODULE