SRR3548465_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241552
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	3851	1.594273696760946	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2436	1.0084785056633767	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	1583	0.6553454328674572	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	1211	0.5013413260912764	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	764	0.3162880042392528	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	650	0.26909319732397163	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	630	0.26081340663708025	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	554	0.22935020202689277	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	551	0.22810823342385905	No Hit
AGCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	524	0.21693051599655563	No Hit
AGCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	484	0.20037093462277272	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT	483	0.19995694508842818	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC	441	0.18256938464595615	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	413	0.17097767768430816	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	406	0.16807975094389616	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	404	0.16725177187520698	No Hit
GCTCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	365	0.1511061800357687	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	355	0.14696628469232298	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	353	0.14613830562363384	No Hit
GCCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	341	0.14117043121149897	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	317	0.13123468238722924	No Hit
CATGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTA	291	0.12047095449427038	No Hit
GCTCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	280	0.11591706961648009	No Hit
TACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	277	0.11467510101344638	No Hit
AGCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	277	0.11467510101344638	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	269	0.1113631847386898	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	264	0.10929323706696695	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.10473935218917665	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	250	0.10349738358614294	No Hit
GCCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGT	247	0.10225541498310924	No Hit
GCTTATGGGATTATTTTGAGACTGGATTTTGGTGAAAAACAATTCATCCGG	247	0.10225541498310924	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	243	0.10059945684573093	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCA	35	1.2066084E-7	45.000004	5
TCGTTGA	35	1.2066084E-7	45.000004	24
GCGACGG	35	1.2066084E-7	45.000004	2
AATCACA	35	1.2066084E-7	45.000004	43
GGTAACT	35	1.2066084E-7	45.000004	9
TCACATT	35	1.2066084E-7	45.000004	42
ATTACGG	35	1.2066084E-7	45.000004	2
ACTAGTG	35	1.2066084E-7	45.000004	39
ACAATTC	35	1.2066084E-7	45.000004	39
TCCACAC	35	1.2066084E-7	45.000004	26
ATCTTAT	35	1.2066084E-7	45.000004	15
CGGGAGA	65	0.0	45.000004	6
ACCACGC	35	1.2066084E-7	45.000004	34
CGCACAT	35	1.2066084E-7	45.000004	38
ACTCCAC	35	1.2066084E-7	45.000004	24
TTCATCC	35	1.2066084E-7	45.000004	43
AGTACGG	35	1.2066084E-7	45.000004	2
AGTACAG	35	1.2066084E-7	45.000004	1
TAGTAGA	65	0.0	45.000004	27
TAATCGT	35	1.2066084E-7	45.000004	21

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content