##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548465_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241552
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44734053123137	31.0	31.0	34.0	30.0	34.0
2	31.64601410876333	31.0	31.0	34.0	30.0	34.0
3	31.855149201828176	33.0	31.0	34.0	30.0	34.0
4	35.57157051069749	37.0	35.0	37.0	33.0	37.0
5	35.346952209048155	37.0	35.0	37.0	33.0	37.0
6	35.36155775981983	37.0	35.0	37.0	33.0	37.0
7	35.45509869510499	37.0	35.0	37.0	33.0	37.0
8	35.497255249387294	37.0	35.0	37.0	33.0	37.0
9	37.02967476982182	39.0	37.0	39.0	33.0	39.0
10	36.66584006756309	38.0	35.0	39.0	32.0	39.0
11	36.78024607537922	39.0	37.0	39.0	32.0	39.0
12	36.90284907597536	39.0	37.0	39.0	33.0	39.0
13	36.8367225276545	39.0	37.0	39.0	33.0	39.0
14	37.88304381665232	39.0	37.0	41.0	33.0	41.0
15	37.934908425515005	40.0	37.0	41.0	33.0	41.0
16	37.79847817447175	39.0	37.0	41.0	33.0	41.0
17	37.73276975558058	39.0	37.0	41.0	33.0	41.0
18	37.64753759024972	39.0	37.0	41.0	33.0	41.0
19	37.58432966814599	39.0	36.0	41.0	32.0	41.0
20	37.4675059614493	39.0	36.0	40.0	32.0	41.0
21	37.37793104590316	39.0	36.0	40.0	32.0	41.0
22	37.3243152613102	39.0	36.0	40.0	32.0	41.0
23	37.143422534278336	39.0	36.0	40.0	31.0	41.0
24	37.07317679009075	39.0	35.0	40.0	31.0	41.0
25	37.04217725375902	39.0	35.0	40.0	31.0	41.0
26	37.05811585083129	39.0	35.0	40.0	31.0	41.0
27	37.00267023249652	39.0	36.0	40.0	31.0	41.0
28	36.76099556203219	39.0	35.0	40.0	31.0	41.0
29	36.55917152414387	39.0	35.0	40.0	30.0	41.0
30	36.5841019739021	39.0	35.0	40.0	30.0	41.0
31	36.48585397761145	39.0	35.0	40.0	30.0	41.0
32	36.48625140756442	39.0	35.0	40.0	30.0	41.0
33	36.32681575809764	39.0	35.0	40.0	30.0	41.0
34	36.40786248923627	39.0	35.0	40.0	30.0	41.0
35	36.28269275352719	39.0	35.0	40.0	30.0	41.0
36	36.18285089752931	39.0	35.0	40.0	29.0	41.0
37	36.109053123137045	39.0	35.0	40.0	29.0	41.0
38	36.04480194740677	39.0	35.0	40.0	29.0	41.0
39	35.93758693780221	39.0	35.0	40.0	28.0	41.0
40	35.844857422004374	39.0	35.0	40.0	28.0	41.0
41	35.85381615552759	39.0	35.0	40.0	28.0	41.0
42	35.721674836060146	38.0	35.0	40.0	27.0	41.0
43	35.643799264754584	38.0	35.0	40.0	27.0	41.0
44	35.590477412731005	38.0	35.0	40.0	27.0	41.0
45	35.421681459892696	38.0	35.0	40.0	26.0	41.0
46	35.23643770285487	38.0	34.0	40.0	26.0	41.0
47	35.11162813804067	38.0	34.0	40.0	25.0	41.0
48	35.10749238259257	38.0	34.0	40.0	25.0	41.0
49	34.8730087103398	38.0	34.0	40.0	24.0	41.0
50	34.82360733920647	38.0	34.0	40.0	24.0	41.0
51	33.475806451612904	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	1.0
15	10.0
16	28.0
17	64.0
18	173.0
19	302.0
20	638.0
21	1001.0
22	1333.0
23	1610.0
24	1829.0
25	2068.0
26	2183.0
27	2474.0
28	3044.0
29	3775.0
30	4966.0
31	6156.0
32	8081.0
33	10415.0
34	14542.0
35	19748.0
36	23226.0
37	33220.0
38	50631.0
39	50022.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.52104722792608	34.07589256143604	18.90855799165397	26.494502218983907
2	19.517122607140493	35.0404881764589	20.191925548122143	25.250463668278467
3	20.055309001788434	33.086457574352515	20.896535735576606	25.96169768828244
4	17.13999470093396	32.57766443664304	19.427700867722063	30.854639994700932
5	15.046035636219116	36.48820957806186	17.874826124395575	30.590928661323442
6	19.056766244949326	38.3925614360469	18.684589653573557	23.86608266543022
7	75.95962774061071	8.48388752732331	9.065956812611777	6.4905279194541965
8	78.58183745114924	7.591740080810758	6.6018911041928865	7.224531363847123
9	69.5432039478042	13.034460488838842	12.658144002119625	4.764191561237332
10	23.135805126846392	46.26291647347155	14.154716168775252	16.446562230906803
11	15.708004901636086	32.49403855070544	35.31786116446976	16.480095383188715
12	19.429356825859443	23.081572497847255	38.45921375107637	19.029856925216933
13	25.469464131946744	26.007650526594688	29.067033185401076	19.455852156057496
14	16.05989600582897	32.44932768099623	33.28103265549447	18.209743657680335
15	14.307064317414056	32.87325296416507	33.579933099291246	19.23974961912963
16	15.999039544280322	34.42778366562893	27.652016957011327	21.92115983307942
17	14.436643041663908	31.220192753527193	29.30838908392396	25.034775120884944
18	19.444260449095847	27.62717758495065	26.65720010598132	26.27136185997218
19	18.470143074783067	33.90739882095781	28.974713519242236	18.64774458501689
20	18.748344041862623	30.571057163674904	27.595714380340464	23.084884414122012
21	19.14908591110817	31.230128502351462	27.532787971120086	22.08799761542028
22	15.356527786977544	31.579949658872625	28.067662449493273	24.995860104656554
23	18.649400543154268	28.693200635887923	26.61994104789031	26.037457773067494
24	14.729747631979864	32.70186129694641	25.984881102205737	26.583509968867986
25	16.770302046764257	32.12558786513877	27.770003974299527	23.334106113797443
26	19.763032390541166	29.83995164602239	25.839570775650795	24.557445187785653
27	15.039411803669603	28.515599125654102	27.857355766046233	28.58763330463006
28	19.392097767768433	27.414800953831886	31.719878121481088	21.473223156918593
29	19.512568722262703	28.9502881367159	25.060856461548653	26.476286679472743
30	18.343048287739286	29.103464264423394	29.493442405775983	23.060045042061336
31	20.922203086705967	29.717410743856394	23.316304563820626	26.04408160561701
32	19.676922567397494	25.45538848777903	26.679141551301583	28.18854739352189
33	18.405146717890972	30.31893753725906	28.38229449559515	22.89362124925482
34	17.72040802808505	24.066453600052988	28.39885407696893	29.814284294893024
35	17.479880108630855	26.191461879843676	29.89832417036497	26.430333841160497
36	17.93319864873816	24.773961714247864	33.49961912962841	23.793220507385573
37	20.247400145724317	26.58144002119626	27.362224282970125	25.80893555010929
38	20.62206067430615	26.252732330926676	29.484334636020403	23.64087235874677
39	19.54858581175068	24.917616082665432	27.811816917268334	27.72198118831556
40	20.64524408822945	26.855915082466712	26.536315161952707	25.962525667351127
41	17.95845201033318	21.856577465721667	32.40461681128701	27.780353712658147
42	22.37116645691197	24.550821355236142	30.159551566536397	22.918460621315493
43	18.182834337947938	23.027339868848117	30.68035040074187	28.10947539246208
44	21.819318407630657	20.641932171954693	29.608531496323774	27.93021792409088
45	23.712906537722727	23.122971451281714	28.34876134331324	24.81536066768232
46	17.514655229515796	24.208038020798835	32.254338610319934	26.02296813936544
47	19.776280055640193	24.61416175399086	27.09602901238657	28.513529177982385
48	18.688729548917003	20.57817778366563	34.135921043916014	26.59717162350136
49	20.446529111744056	21.16314499569451	32.118136053520566	26.27218983904087
50	19.241405577267006	20.908541432072596	28.606262833675565	31.24379015698483
51	19.35732264688349	19.743574882426973	34.68238722925084	26.2167152414387
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2847.0
1	2243.0
2	1639.0
3	1117.0
4	595.0
5	540.0
6	485.0
7	544.5
8	604.0
9	612.0
10	620.0
11	736.0
12	852.0
13	909.0
14	966.0
15	1004.5
16	1043.0
17	1096.0
18	1149.0
19	1119.5
20	1090.0
21	1277.5
22	1465.0
23	1551.0
24	1637.0
25	1780.5
26	2173.5
27	2423.0
28	2527.0
29	2631.0
30	3432.5
31	4234.0
32	4175.5
33	4117.0
34	4308.0
35	4499.0
36	5581.5
37	6664.0
38	7032.5
39	7401.0
40	8188.0
41	8975.0
42	11599.0
43	14223.0
44	17814.0
45	21405.0
46	23493.0
47	25581.0
48	28911.5
49	32242.0
50	29484.0
51	26726.0
52	22768.5
53	18811.0
54	16138.0
55	13465.0
56	11465.0
57	9465.0
58	8289.0
59	7113.0
60	6289.5
61	5466.0
62	4346.0
63	3226.0
64	2548.5
65	1871.0
66	1752.0
67	1633.0
68	1283.5
69	934.0
70	810.0
71	686.0
72	533.5
73	381.0
74	296.5
75	131.0
76	50.0
77	77.0
78	104.0
79	75.0
80	46.0
81	30.5
82	15.0
83	21.0
84	27.0
85	17.0
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241552.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.39920182817779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.77854486311887	27.94677750546466
2	10.47393742251743	7.624859243558323
3	3.968244941596624	4.333228455984632
4	1.955119820752249	2.8465920381532754
5	1.1441829782878201	2.082367357753196
6	0.7529315423723031	1.644366430416639
7	0.5459322361611865	1.391004835397761
8	0.41854804772357634	1.2187851891104193
9	0.30367480636466	0.9948168510300059
>10	3.0208250400918986	24.039130290786247
>50	0.49702580667174684	12.402712459429026
>100	0.1296589060882818	8.195336821885142
>500	0.006824152952014831	1.520583559647612
>1k	0.0045494353013432205	3.759438961383056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGCT	3851	1.594273696760946	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2436	1.0084785056633767	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	1583	0.6553454328674572	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	1211	0.5013413260912764	No Hit
CCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	764	0.3162880042392528	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	650	0.26909319732397163	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	630	0.26081340663708025	No Hit
GCCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	554	0.22935020202689277	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	551	0.22810823342385905	No Hit
AGCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	524	0.21693051599655563	No Hit
AGCCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	484	0.20037093462277272	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGT	483	0.19995694508842818	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCC	441	0.18256938464595615	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	413	0.17097767768430816	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	406	0.16807975094389616	No Hit
AACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	404	0.16725177187520698	No Hit
GCTCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTC	365	0.1511061800357687	No Hit
ACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTG	355	0.14696628469232298	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	353	0.14613830562363384	No Hit
GCCCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	341	0.14117043121149897	No Hit
GAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTT	317	0.13123468238722924	No Hit
CATGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTA	291	0.12047095449427038	No Hit
GCTCCAGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	280	0.11591706961648009	No Hit
TACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	277	0.11467510101344638	No Hit
AGCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACT	277	0.11467510101344638	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	269	0.1113631847386898	No Hit
GAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	264	0.10929323706696695	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.10473935218917665	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCT	250	0.10349738358614294	No Hit
GCCCCAGGGAATGATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGT	247	0.10225541498310924	No Hit
GCTTATGGGATTATTTTGAGACTGGATTTTGGTGAAAAACAATTCATCCGG	247	0.10225541498310924	No Hit
TCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	243	0.10059945684573093	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7867788302311718	0.0
2	0.0	0.0	0.0	2.4988408293038353	0.0
3	0.0	0.0	0.0	3.2643074783069483	0.0
4	0.0	0.0	0.0	6.902447506127045	0.0
5	0.0	0.0	0.0	7.472925084453865	0.0
6	0.0	0.0	0.0	10.051665893886202	0.0
7	0.0	0.0	0.0	12.118715638868649	0.0
8	0.0	0.0	0.0	13.568092998608995	0.0
9	0.0	0.0	0.0	15.58794793667616	0.0
10	0.0	0.0	0.0	18.62124925481884	0.0
11	0.0	0.0	0.0	21.06544346558919	0.0
12	0.0	0.0	0.0	22.514820825329537	0.0
13	0.0	0.0	0.0	23.260001987149764	0.0
14	0.0	0.0	0.0	23.810608067828046	0.0
15	0.0	0.0	0.0	24.440286149566138	0.0
16	0.0	0.0	0.0	25.624710207325958	0.0
17	0.0	0.0	0.0	27.073259587997615	0.0
18	0.0	0.0	0.0	29.687603497383588	0.0
19	0.0	0.0	0.0	30.42864476386037	0.0
20	0.0	0.0	0.0	31.750099357488242	0.0
21	0.0	0.0	0.0	32.50604424720143	0.0
22	0.0	0.0	0.0	33.41930515996555	0.0
23	0.0	0.0	0.0	34.29696297277605	0.0
24	0.0	0.0	0.0	34.84756905345433	0.0
25	0.0	0.0	0.0	35.32075909121017	0.0
26	0.0	0.0	0.0	35.772421673180105	0.0
27	0.0	0.0	0.0	36.546168112870106	0.0
28	0.0	0.0	0.0	37.10836590051004	0.0
29	0.0	0.0	0.0	37.605567331257866	0.0
30	0.0	0.0	0.0	38.43230443134397	0.0
31	0.0	0.0	0.0	38.93281777836656	0.0
32	0.0	0.0	0.0	39.41884149168709	0.0
33	0.0	0.0	0.0	39.88209578061866	0.0
34	0.0	0.0	0.0	40.322166655626944	0.0
35	0.0	0.0	0.0	40.83013181426774	0.0
36	0.0	0.0	0.0	41.28841822878718	0.0
37	0.0	0.0	0.0	41.768232099092536	0.0
38	0.0	0.0	0.0	42.19836722527654	0.0
39	0.0	0.0	0.0	42.64257799562827	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCA	35	1.2066084E-7	45.000004	5
TCGTTGA	35	1.2066084E-7	45.000004	24
GCGACGG	35	1.2066084E-7	45.000004	2
AATCACA	35	1.2066084E-7	45.000004	43
GGTAACT	35	1.2066084E-7	45.000004	9
TCACATT	35	1.2066084E-7	45.000004	42
ATTACGG	35	1.2066084E-7	45.000004	2
ACTAGTG	35	1.2066084E-7	45.000004	39
ACAATTC	35	1.2066084E-7	45.000004	39
TCCACAC	35	1.2066084E-7	45.000004	26
ATCTTAT	35	1.2066084E-7	45.000004	15
CGGGAGA	65	0.0	45.000004	6
ACCACGC	35	1.2066084E-7	45.000004	34
CGCACAT	35	1.2066084E-7	45.000004	38
ACTCCAC	35	1.2066084E-7	45.000004	24
TTCATCC	35	1.2066084E-7	45.000004	43
AGTACGG	35	1.2066084E-7	45.000004	2
AGTACAG	35	1.2066084E-7	45.000004	1
TAGTAGA	65	0.0	45.000004	27
TAATCGT	35	1.2066084E-7	45.000004	21
>>END_MODULE