##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548464_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481869
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.635075093023207	31.0	31.0	34.0	30.0	34.0
2	31.84318559608524	33.0	31.0	34.0	30.0	34.0
3	32.03343024763992	33.0	31.0	34.0	30.0	34.0
4	35.72783681872044	37.0	35.0	37.0	33.0	37.0
5	35.50087886956828	37.0	35.0	37.0	33.0	37.0
6	34.81361739393902	37.0	35.0	37.0	32.0	37.0
7	35.35154990256688	37.0	35.0	37.0	33.0	37.0
8	35.767650544027525	37.0	35.0	37.0	35.0	37.0
9	37.35944623953813	39.0	37.0	39.0	35.0	39.0
10	36.81757075055668	39.0	37.0	39.0	32.0	39.0
11	36.87807474645599	39.0	37.0	39.0	33.0	39.0
12	36.93713021588855	39.0	37.0	39.0	33.0	39.0
13	36.916101679087056	39.0	37.0	39.0	33.0	39.0
14	38.067190460477846	40.0	37.0	41.0	33.0	41.0
15	38.069344572902594	40.0	37.0	41.0	33.0	41.0
16	37.94379177743328	40.0	37.0	41.0	33.0	41.0
17	37.942926397008314	40.0	37.0	41.0	33.0	41.0
18	37.91147594055646	40.0	37.0	41.0	33.0	41.0
19	37.94198215697627	40.0	37.0	41.0	33.0	41.0
20	37.82609796438451	40.0	37.0	41.0	33.0	41.0
21	37.765660376575376	39.0	37.0	41.0	33.0	41.0
22	37.7668017656251	39.0	37.0	41.0	33.0	41.0
23	37.62088866476159	39.0	37.0	41.0	32.0	41.0
24	37.54296292145791	39.0	36.0	41.0	32.0	41.0
25	37.510007906713234	39.0	36.0	41.0	32.0	41.0
26	37.508839539376886	39.0	36.0	41.0	32.0	41.0
27	37.424953669980844	39.0	36.0	41.0	32.0	41.0
28	37.29403427072503	39.0	36.0	41.0	32.0	41.0
29	37.061444085425705	39.0	36.0	41.0	31.0	41.0
30	37.126055006651185	39.0	36.0	41.0	31.0	41.0
31	37.06088791767057	39.0	36.0	41.0	31.0	41.0
32	37.01047795147644	39.0	36.0	41.0	31.0	41.0
33	36.853644870286324	39.0	36.0	41.0	31.0	41.0
34	36.78398693420826	39.0	35.0	41.0	30.0	41.0
35	36.81001890555317	39.0	35.0	41.0	30.0	41.0
36	36.73432198377567	39.0	35.0	40.0	30.0	41.0
37	36.687981588357005	39.0	35.0	40.0	30.0	41.0
38	36.524812760314525	39.0	35.0	40.0	30.0	41.0
39	36.48360654036678	39.0	35.0	40.0	30.0	41.0
40	36.37540908421168	39.0	35.0	40.0	30.0	41.0
41	36.40530102579747	39.0	35.0	40.0	30.0	41.0
42	36.33983094990547	39.0	35.0	40.0	30.0	41.0
43	36.31539899848299	39.0	35.0	40.0	30.0	41.0
44	36.22325154761979	39.0	35.0	40.0	30.0	41.0
45	36.01713328726272	38.0	35.0	40.0	29.0	41.0
46	35.82496695159888	38.0	35.0	40.0	28.0	41.0
47	35.715233808358704	38.0	35.0	40.0	28.0	41.0
48	35.58982420533381	38.0	34.0	40.0	28.0	41.0
49	35.57316822621916	38.0	34.0	40.0	28.0	41.0
50	35.44641385936842	38.0	34.0	40.0	27.0	41.0
51	34.31082721652565	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	1.0
14	2.0
15	9.0
16	29.0
17	65.0
18	140.0
19	307.0
20	637.0
21	1003.0
22	1570.0
23	1915.0
24	2331.0
25	2786.0
26	3356.0
27	4209.0
28	5338.0
29	6819.0
30	8912.0
31	11666.0
32	15090.0
33	20390.0
34	29034.0
35	37355.0
36	46878.0
37	67427.0
38	101020.0
39	113551.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.898289784152954	32.0634861341983	21.828546762709365	27.20967731893938
2	23.579437564981355	29.760578082424892	21.114659793429336	25.545324559164424
3	19.760557329896713	31.323866029979104	20.123311522426217	28.792265117697962
4	19.433705011112977	32.15064675254063	18.721063193523552	29.69458504282284
5	18.19996721100548	32.792522449047354	17.88722661138193	31.120283728565234
6	18.63327999933592	37.832688967333446	18.373250821281303	25.160780212049332
7	85.4701173970519	4.733651677115565	5.381130556230013	4.415100369602527
8	86.94064154365606	4.188897812476005	4.031593648896277	4.838866994971663
9	79.13893610089049	8.692611477393234	8.38837941432215	3.780073007394126
10	27.929582521390667	46.89012989007386	11.805283178623235	13.37500440991224
11	20.215037696967432	29.259612052238264	33.66931676451484	16.856033486279465
12	22.52188872909442	24.127926884692727	35.263110928488864	18.08707345772399
13	25.42537494630283	25.467502578501627	29.466929808723947	19.64019266647159
14	18.471202754275538	30.593792088721212	30.215058449495608	20.719946707507642
15	16.55823470694317	31.153280248366254	32.25357929229729	20.034905752393286
16	20.192209915973013	32.14006296317049	27.176888324420123	20.490838796436375
17	18.739325418319087	29.385372372989337	29.043785759200112	22.83151644949146
18	22.469592358089024	26.722200432067638	27.485270893126557	23.322936316716785
19	21.188538793738548	31.211802377824675	28.16242588753375	19.437232940903026
20	22.796859727436296	29.077197329564676	27.74613017230824	20.37981277069079
21	22.726301131635363	30.356383166379242	27.380885676397526	19.536430025587865
22	21.223818091639014	27.368849210055014	28.359159854649292	23.04817284365668
23	20.586715476612937	29.34552751888999	26.66637613127219	23.40138087322488
24	17.631347938962662	29.40965283095613	28.30707100892566	24.651928221155543
25	19.02405010490403	31.28319107475268	25.822163285042198	23.870595535301085
26	21.693447804278758	29.186148102492588	25.305632858723015	23.814771234505645
27	18.253508733701484	30.049660799926954	26.33973133777022	25.35709912860134
28	19.807042993012626	28.99792267192951	28.38074248395311	22.814291851104763
29	20.67761155002708	30.454127574091714	25.23673446517622	23.631526410704986
30	21.331938763439855	29.134266782050723	26.449719737106975	23.084074717402448
31	22.714057140011082	28.443207593765113	24.562069774150235	24.280665492073574
32	20.692138319750804	29.269365740481334	25.884835920135966	24.153660019631893
33	22.234466213846503	27.7616945684408	26.054176550058212	23.949662667654486
34	19.412329907090932	27.32713662841976	28.799528502559824	24.461004961929486
35	20.233714972326503	27.407033861900228	28.17446235387626	24.18478881189701
36	20.26090078423804	29.76576621446908	28.585362411775815	21.387970589517067
37	21.191236622401526	29.83819253780592	25.93215168437895	23.03841915541361
38	22.037109670885656	29.284307560768593	28.47890194222911	20.19968082611664
39	21.923385816477094	26.809983626255267	27.501665390386183	23.764965166881456
40	23.54810124743447	26.42377907688604	27.480290286364134	22.547829389315353
41	18.886253317810443	24.323623225399434	30.86399000558243	25.926133451207694
42	23.008535514839096	26.63503981372531	27.232712625215566	23.123712046220028
43	19.7912710715983	26.558670510034887	28.19853528656128	25.45152313180553
44	22.51151246500605	26.047120690478113	25.511497938236328	25.92986890627951
45	24.439007282062136	25.675857961396147	26.95545884877425	22.929675907767464
46	20.275220028679993	26.587931574764095	29.13883233824961	23.998016058306305
47	21.43258852509707	26.254438446963803	27.15987125131519	25.153101776623938
48	19.784007686736437	25.258939670325336	32.24465570518129	22.712396937756942
49	22.30377965795683	24.62868538959759	28.877142957940848	24.190391994504733
50	20.180796025475804	25.176344608181893	27.844497155866016	26.79836221047629
51	20.494989302071726	23.02202465815398	30.859839499947086	25.623146539827214
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2104.0
1	1665.0
2	1226.0
3	839.0
4	452.0
5	421.0
6	390.0
7	452.5
8	515.0
9	553.5
10	592.0
11	689.0
12	786.0
13	795.5
14	805.0
15	876.5
16	948.0
17	1093.5
18	1239.0
19	1407.0
20	1575.0
21	1950.0
22	2325.0
23	2771.5
24	3218.0
25	3340.0
26	4551.5
27	5641.0
28	6403.0
29	7165.0
30	9075.0
31	10985.0
32	12036.0
33	13087.0
34	13525.5
35	13964.0
36	14878.5
37	15793.0
38	17380.5
39	18968.0
40	20730.0
41	22492.0
42	24411.0
43	26330.0
44	30712.5
45	35095.0
46	39272.0
47	43449.0
48	48238.0
49	53027.0
50	50940.0
51	48853.0
52	43433.0
53	38013.0
54	33511.0
55	29009.0
56	25660.5
57	22312.0
58	19507.5
59	16703.0
60	15397.5
61	14092.0
62	12230.5
63	10369.0
64	8504.5
65	6640.0
66	5299.0
67	3958.0
68	3052.5
69	2147.0
70	1767.0
71	1387.0
72	1163.5
73	940.0
74	937.5
75	713.5
76	492.0
77	315.5
78	139.0
79	134.0
80	129.0
81	82.0
82	35.0
83	42.0
84	49.0
85	32.0
86	15.0
87	12.5
88	10.0
89	9.0
90	8.0
91	4.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	481869.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.55331193163996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22849150698632	23.737080586666668
2	10.450456923513753	6.594930542715918
3	3.811889100858616	3.608331775446315
4	1.9148748603645807	2.416825751165565
5	1.0820568043009304	1.7071237936930377
6	0.775113850985772	1.4674445473613256
7	0.5870570604386424	1.2966516184784465
8	0.4469808328170062	1.1282980516271366
9	0.3757653323155404	1.0670976669283736
>10	4.4427877231756705	32.225508206856915
>50	0.7552954828056653	15.98882856198405
>100	0.12253463949772761	6.323739414732938
>500	0.005356705551813229	1.1599121107508548
>1k	0.0013391763879533071	1.2782273715924721
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGCT	4271	0.8863404784287846	TruSeq Adapter, Index 15 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1779	0.36918747626429593	No Hit
AAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	939	0.1948662395796368	No Hit
TGCCCCGGGAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTC	918	0.1905082086625203	No Hit
CCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	711	0.14755047533665788	TruSeq Adapter, Index 14 (95% over 21bp)
TGCCCCGGGAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTT	635	0.1317785539223316	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTT	625	0.12970330110465708	No Hit
GCGAGCGGGATTCCTTTGTACTCTCACCTCTCACTTCTCTCTCCTCTTCCC	590	0.12243991624279627	No Hit
AATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCT	558	0.11579910722623783	No Hit
AGCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTC	514	0.10666799482846998	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0752528176745132E-4	0.0	0.0	1.0289103470030236	0.0
2	2.0752528176745132E-4	0.0	0.0	1.4057762586927152	0.0
3	2.0752528176745132E-4	0.0	0.0	1.8424094515314329	0.0
4	2.0752528176745132E-4	0.0	0.0	3.8259360946647325	0.0
5	2.0752528176745132E-4	0.0	0.0	4.117301590266234	0.0
6	2.0752528176745132E-4	0.0	0.0	5.575374219964347	0.0
7	2.0752528176745132E-4	0.0	0.0	6.655335786282164	0.0
8	2.0752528176745132E-4	0.0	0.0	7.568031975495415	0.0
9	2.0752528176745132E-4	0.0	0.0	8.820032000398449	0.0
10	2.0752528176745132E-4	0.0	0.0	10.658290946294532	0.0
11	2.0752528176745132E-4	0.0	0.0	12.278440821053024	0.0
12	2.0752528176745132E-4	0.0	0.0	13.212097063724789	0.0
13	2.0752528176745132E-4	0.0	0.0	13.598301613094016	0.0
14	2.0752528176745132E-4	0.0	0.0	13.972884746684265	0.0
15	2.0752528176745132E-4	0.0	0.0	14.303680045821583	0.0
16	2.0752528176745132E-4	0.0	0.0	15.093313742946734	0.0
17	2.0752528176745132E-4	0.0	0.0	16.092547974657013	0.0
18	2.0752528176745132E-4	0.0	0.0	17.696718402719412	0.0
19	2.0752528176745132E-4	0.0	0.0	18.21221120262976	0.0
20	2.0752528176745132E-4	0.0	0.0	19.15105557734571	0.0
21	2.0752528176745132E-4	0.0	0.0	19.7829700603276	0.0
22	2.0752528176745132E-4	0.0	0.0	20.554341532657215	0.0
23	2.0752528176745132E-4	0.0	0.0	21.25598451031297	0.0
24	2.0752528176745132E-4	0.0	0.0	21.742838821339408	0.0
25	2.0752528176745132E-4	0.0	0.0	22.17179357875273	0.0
26	2.0752528176745132E-4	0.0	0.0	22.55364009720484	0.0
27	2.0752528176745132E-4	0.0	0.0	23.121014217557054	0.0
28	2.0752528176745132E-4	0.0	0.0	23.625715702815494	0.0
29	2.0752528176745132E-4	0.0	0.0	24.064424148471886	0.0
30	2.0752528176745132E-4	0.0	0.0	24.93561528133165	0.0
31	2.0752528176745132E-4	0.0	0.0	25.366437766280878	0.0
32	2.0752528176745132E-4	0.0	0.0	25.774224944953918	0.0
33	2.0752528176745132E-4	0.0	0.0	26.198614146168357	0.0
34	2.0752528176745132E-4	0.0	0.0	26.61345718442149	0.0
35	2.0752528176745132E-4	0.0	0.0	27.090557807204863	0.0
36	2.0752528176745132E-4	0.0	0.0	27.514531957855766	0.0
37	2.0752528176745132E-4	0.0	0.0	27.960088737810484	0.0
38	2.0752528176745132E-4	0.0	0.0	28.38385536317962	0.0
39	2.0752528176745132E-4	0.0	0.0	28.8283745167255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAC	20	7.029204E-4	45.0	39
GAGTGCG	20	7.029204E-4	45.0	1
CAGCGCG	25	3.887422E-5	45.0	1
AACCGCG	25	3.887422E-5	45.0	1
TCGTTGA	50	2.1827873E-11	45.0	24
TCCATAC	25	3.887422E-5	45.0	33
CGTATAG	70	0.0	45.0	1
CGCGGGT	45	3.8380676E-10	45.0	4
CGTGCGG	55	1.8189894E-12	45.0	2
CCGATAC	20	7.029204E-4	45.0	37
GAGTCCC	25	3.887422E-5	45.0	9
TGGACGG	20	7.029204E-4	45.0	2
GCGCGAC	40	6.8012014E-9	45.0	9
CGGCATG	20	7.029204E-4	45.0	6
GGCCGTA	25	3.887422E-5	45.0	42
CGGCAGA	20	7.029204E-4	45.0	15
CACGCTA	20	7.029204E-4	45.0	20
CGACCTC	40	6.8012014E-9	45.0	12
CGTTCGC	20	7.029204E-4	45.0	18
TCGGCGG	25	3.887422E-5	45.0	2
>>END_MODULE