FastQCFastQC Report
Sat 18 Jun 2016
SRR3548460_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548460_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296046
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGT53181.7963424602933327No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC52761.782155475838214No Hit
AATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT49041.6564993278071654No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT19940.6735439762739573No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18820.6357120177269748No Hit
AATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT12850.434054167257791No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT10510.35501239672213103No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCATC9860.33305634935111433No Hit
AACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG9330.31515372611013154No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTAT8090.2732683434331151No Hit
AAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT8000.27022827533558974No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT5390.1820663005073536No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC5230.1766617350006418No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.14355877127203207No Hit
AATGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC3670.12396722131020178No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3460.11687372908264257No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT3150.10640238341338847No Hit
ACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG3140.10606459806921899No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG207.024793E-445.02
GCCCATA207.024793E-445.025
GGTACGA207.024793E-445.09
ACGCCTC207.024793E-445.045
CTCGTCA207.024793E-445.036
GTATTCG207.024793E-445.014
ATTAGCG207.024793E-445.01
TTAGCGG502.1827873E-1145.02
CTATCAT253.8837665E-545.027
GCGCGAC207.024793E-445.09
ATTACGG253.8837665E-545.02
ATTACCC207.024793E-445.014
CCTACCG207.024793E-445.01
CCAGCGG502.1827873E-1145.02
ATTCGGA207.024793E-445.016
CGTCTAC207.024793E-445.040
CGATTGG253.8837665E-545.02
ATATGCG207.024793E-445.01
CGTTCTA207.024793E-445.027
CCTAAGG406.7848305E-945.02