##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548460_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 296046 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.576619849617966 31.0 31.0 34.0 30.0 34.0 2 31.77629152226343 31.0 31.0 34.0 30.0 34.0 3 31.94120508299386 33.0 31.0 34.0 30.0 34.0 4 35.630216250177334 37.0 35.0 37.0 33.0 37.0 5 35.35853887571526 37.0 35.0 37.0 33.0 37.0 6 34.795366260648684 37.0 35.0 37.0 32.0 37.0 7 35.069300041209814 37.0 35.0 37.0 32.0 37.0 8 35.51088344378914 37.0 35.0 37.0 33.0 37.0 9 37.226812725049484 39.0 37.0 39.0 34.0 39.0 10 36.71506117292583 38.0 37.0 39.0 32.0 39.0 11 36.71202448268175 39.0 35.0 39.0 32.0 39.0 12 36.68714321423022 39.0 35.0 39.0 32.0 39.0 13 36.70960593961749 39.0 35.0 39.0 32.0 39.0 14 37.79314701093749 39.0 37.0 41.0 33.0 41.0 15 37.9197962478804 40.0 37.0 41.0 33.0 41.0 16 37.68374509366787 39.0 37.0 41.0 32.0 41.0 17 37.67176046965674 39.0 37.0 41.0 32.0 41.0 18 37.55994676502976 39.0 36.0 41.0 32.0 41.0 19 37.45237902217899 39.0 36.0 41.0 32.0 41.0 20 37.51202178039899 39.0 36.0 41.0 32.0 41.0 21 37.55138052870162 39.0 36.0 41.0 32.0 41.0 22 37.49412253501145 39.0 36.0 41.0 32.0 41.0 23 37.342662964539294 39.0 36.0 41.0 32.0 41.0 24 37.2235733635989 39.0 36.0 41.0 31.0 41.0 25 37.26783675509887 39.0 36.0 41.0 32.0 41.0 26 37.19727339670187 39.0 36.0 41.0 32.0 41.0 27 37.11314457888301 39.0 36.0 41.0 31.0 41.0 28 37.06068651493349 39.0 36.0 41.0 31.0 41.0 29 36.826885011113134 39.0 35.0 40.0 31.0 41.0 30 36.95021381812286 39.0 36.0 40.0 31.0 41.0 31 36.79989933996744 39.0 35.0 40.0 31.0 41.0 32 36.72125277828446 39.0 35.0 40.0 30.0 41.0 33 36.54709741053755 39.0 35.0 40.0 30.0 41.0 34 36.513626260783795 39.0 35.0 40.0 30.0 41.0 35 36.445069347331156 39.0 35.0 40.0 30.0 41.0 36 36.47540584909102 39.0 35.0 40.0 30.0 41.0 37 36.385784641575974 39.0 35.0 40.0 30.0 41.0 38 36.212618984887484 39.0 35.0 40.0 30.0 41.0 39 36.18951108949285 39.0 35.0 40.0 30.0 41.0 40 35.97010261918756 39.0 35.0 40.0 29.0 41.0 41 36.034673665579 39.0 35.0 40.0 29.0 41.0 42 35.91132121359519 39.0 35.0 40.0 28.0 41.0 43 35.939779628841464 39.0 35.0 40.0 29.0 41.0 44 35.87618140424123 39.0 35.0 40.0 28.0 41.0 45 35.584929369084534 38.0 35.0 40.0 27.0 41.0 46 35.340132952311464 38.0 34.0 40.0 26.0 41.0 47 35.276413124987336 38.0 34.0 40.0 26.0 41.0 48 35.18364038021118 38.0 34.0 40.0 26.0 41.0 49 35.0802848206022 38.0 34.0 40.0 26.0 41.0 50 35.02886037980584 38.0 34.0 40.0 26.0 41.0 51 33.601335603250845 37.0 32.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 0.0 13 4.0 14 4.0 15 15.0 16 32.0 17 72.0 18 162.0 19 347.0 20 665.0 21 1074.0 22 1416.0 23 1774.0 24 2059.0 25 2253.0 26 2502.0 27 2833.0 28 3487.0 29 4466.0 30 5897.0 31 7709.0 32 10342.0 33 13156.0 34 18334.0 35 23560.0 36 28206.0 37 39504.0 38 59945.0 39 66214.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.702836721320335 39.565472933260374 16.764286631131647 24.967403714287645 2 18.708579072171215 39.57222864014376 17.580713807989298 24.138478479695724 3 18.762624727238332 30.99619653702465 26.428663113164845 23.812515622572167 4 24.091526316856164 30.027428169946564 17.926943785763022 27.95410172743425 5 16.86764894644751 37.94748113468852 18.83693750295562 26.34793241590834 6 19.712139329698765 33.97715219932038 24.056734426406706 22.253974044574154 7 75.40584909101963 12.006580058504422 7.873438587246577 4.714132263229363 8 77.35216824412421 5.4981320470467425 4.963080061882275 12.186619646946758 9 74.4863973841903 9.222553251859509 10.340284955716342 5.950764408233856 10 29.25085966370091 42.03738608189268 14.164352837059106 14.547401417347306 11 19.919539531018827 26.852921505441724 34.16293413861359 19.06460482492586 12 22.84982739168913 23.216662275457193 36.99762874688393 16.935881585969746 13 23.45615208447336 28.136505813285773 29.030623619302403 19.37671848293846 14 16.648764043425686 28.76546212412936 35.56474331691696 19.021030515527993 15 16.231599143376368 31.376542834559494 28.66108645278099 23.730771569283153 16 18.268782554062543 30.90094106996886 30.55741337494849 20.27286300102011 17 17.26353336981415 30.45776669841849 29.603169777669684 22.67553015409767 18 19.896232342271134 29.814961188463958 25.8800997142336 24.40870675503131 19 18.697769941157794 33.31914634887821 30.046344149220054 17.936739560743938 20 20.253947021746622 33.726853259290785 25.909149253832176 20.11005046513042 21 19.356451362288293 29.242077244752508 28.521918890983155 22.879552501976043 22 20.225573052836385 30.760760152138516 28.742830506070007 20.270836288955092 23 20.270160718266755 29.496767394256295 25.388284253122826 24.84478763435412 24 16.890618349851035 34.57368111712369 25.921985096910618 22.61371543611466 25 19.36455821054836 29.531897070049922 27.33257669416239 23.77096802523932 26 22.98798159745445 31.607588009971423 23.21902677286638 22.185403619707746 27 17.69758753707194 28.78370253271451 28.015916445417265 25.502793484796282 28 23.754416543375015 27.81662309235727 26.85900164163677 21.56995872263094 29 19.881369787127674 30.989440830141262 25.507184694270485 23.622004688460578 30 22.136086959459004 25.797342304912075 24.93767860400073 27.12889213162819 31 22.17527005938266 29.037041540841624 25.82639184451065 22.961296555265058 32 19.765171628733373 26.678624267850264 27.185977854792835 26.370226248623524 33 22.90285969072374 26.147963492160002 28.393560460198753 22.555616356917508 34 18.46841369246671 25.327820676516488 27.169088587584362 29.03467704343244 35 20.47857427561933 27.94734602055086 27.293393594238736 24.280686109591077 36 18.781202921167655 25.42408949960479 30.808725671010585 24.985981908216967 37 19.232146355633922 27.57341764455524 26.70463373935132 26.489802260459523 38 20.57011410388926 26.800564777095452 32.60236584855056 20.026955270464725 39 18.979145132850974 24.31311350263135 31.240077555515022 25.467663809002655 40 21.615897529437994 29.92136357187734 26.55972382670261 21.903015071982058 41 18.884565236483517 24.94342095485161 31.69000763394878 24.48200617471609 42 23.83818730872905 27.67948224262446 24.989359761658662 23.49297068698783 43 20.87344534295346 24.91538477128554 28.01287637731974 26.198293508441257 44 21.81012410233545 23.265303365017598 26.747532478060844 28.17704005458611 45 25.596022239787057 24.95119001776751 28.101038352147977 21.351749390297453 46 18.714996993710436 24.111793437506336 34.72534673665579 22.44786283212744 47 20.948433689359085 26.556345973260914 27.360950663072632 25.13426967430737 48 19.91312160947961 22.84982739168913 33.25125149470015 23.985799504131116 49 21.45984070043169 23.340291711423227 29.61431669402728 25.5855508941178 50 19.590874391141917 22.495490565655338 28.031792356593233 29.88184268660951 51 18.81700816764962 21.956722941705006 33.56978307425197 25.6564858163934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2185.0 1 1743.5 2 1302.0 3 909.5 4 517.0 5 470.5 6 424.0 7 462.5 8 501.0 9 567.0 10 633.0 11 714.0 12 795.0 13 824.5 14 854.0 15 1009.5 16 1165.0 17 1262.5 18 1360.0 19 1329.5 20 1299.0 21 1390.0 22 1481.0 23 1723.5 24 1966.0 25 2157.0 26 2561.5 27 2775.0 28 3290.0 29 3805.0 30 4525.0 31 5245.0 32 5733.0 33 6221.0 34 6918.0 35 7615.0 36 8652.0 37 9689.0 38 10247.5 39 10806.0 40 11536.0 41 12266.0 42 13943.5 43 15621.0 44 24349.0 45 33077.0 46 33346.0 47 33615.0 48 32987.5 49 32360.0 50 30006.5 51 27653.0 52 24092.5 53 20532.0 54 18058.5 55 15585.0 56 13422.0 57 11259.0 58 10187.5 59 9116.0 60 8417.5 61 7719.0 62 6667.5 63 5616.0 64 4463.5 65 3311.0 66 2799.5 67 2288.0 68 1763.0 69 1238.0 70 1019.0 71 800.0 72 585.0 73 370.0 74 371.0 75 266.0 76 160.0 77 95.5 78 31.0 79 25.0 80 19.0 81 23.0 82 27.0 83 19.0 84 11.0 85 7.5 86 4.0 87 4.5 88 5.0 89 3.5 90 2.0 91 1.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 296046.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.0303398796133 #Duplication Level Percentage of deduplicated Percentage of total 1 77.62335736564913 25.639258763840754 2 9.620084880094083 6.355093465204733 3 3.317482231426088 3.287326969457449 4 1.6372654292580662 2.163177344061396 5 1.0062893081761006 1.661903893313877 6 0.7352865981490004 1.4572059747471677 7 0.5123485197116122 1.1846132020023916 8 0.39781152528506414 1.051187991055444 9 0.33236181418417954 0.98802213169575 >10 4.1488980927545125 30.999236605122178 >50 0.5757529273405942 12.339636407855537 >100 0.0797668354042031 3.989582699985813 >500 0.00613591041570793 1.5504347297379462 >1k 0.0051132586797566085 3.754821885788019 >5k 0.0020453034719026437 3.578497936131547 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGT 5318 1.7963424602933327 No Hit AATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC 5276 1.782155475838214 No Hit AATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT 4904 1.6564993278071654 No Hit CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT 1994 0.6735439762739573 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1882 0.6357120177269748 No Hit AATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT 1285 0.434054167257791 No Hit AATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT 1051 0.35501239672213103 No Hit AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCATC 986 0.33305634935111433 No Hit AACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG 933 0.31515372611013154 No Hit AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTAT 809 0.2732683434331151 No Hit AAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT 800 0.27022827533558974 No Hit AAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT 539 0.1820663005073536 No Hit CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC 523 0.1766617350006418 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 425 0.14355877127203207 No Hit AATGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC 367 0.12396722131020178 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 346 0.11687372908264257 No Hit AAAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT 315 0.10640238341338847 No Hit ACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG 314 0.10606459806921899 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.377853441694872E-4 0.0 0.0 0.7603548097255156 0.0 2 3.377853441694872E-4 0.0 0.0 1.158603730501341 0.0 3 3.377853441694872E-4 0.0 0.0 1.9321321686494666 0.0 4 3.377853441694872E-4 0.0 0.0 5.641690818318775 0.0 5 3.377853441694872E-4 0.0 0.0 6.081149551083278 0.0 6 3.377853441694872E-4 0.0 0.0 8.043682400707999 0.0 7 3.377853441694872E-4 0.0 0.0 9.519804354728658 0.0 8 3.377853441694872E-4 0.0 0.0 10.370685636691595 0.0 9 3.377853441694872E-4 0.0 0.0 13.485404295278437 0.0 10 3.377853441694872E-4 0.0 0.0 15.39929605534275 0.0 11 3.377853441694872E-4 0.0 0.0 18.85923133567081 0.0 12 3.377853441694872E-4 0.0 0.0 19.80063908987117 0.0 13 3.377853441694872E-4 0.0 0.0 20.25462259243496 0.0 14 3.377853441694872E-4 0.0 0.0 20.88560561534356 0.0 15 3.377853441694872E-4 0.0 0.0 21.290610243002778 0.0 16 3.377853441694872E-4 0.0 0.0 21.964492004620904 0.0 17 3.377853441694872E-4 0.0 0.0 22.798146234031197 0.0 18 3.377853441694872E-4 0.0 0.0 24.31784249744972 0.0 19 3.377853441694872E-4 0.0 0.0 24.8582990481209 0.0 20 3.377853441694872E-4 0.0 0.0 25.43895205474825 0.0 21 3.377853441694872E-4 0.0 0.0 26.021631773440614 0.0 22 3.377853441694872E-4 0.0 0.0 26.61647176452308 0.0 23 3.377853441694872E-4 0.0 0.0 27.128554346284023 0.0 24 3.377853441694872E-4 0.0 0.0 27.49809151280544 0.0 25 3.377853441694872E-4 0.0 0.0 27.82236544320815 0.0 26 3.377853441694872E-4 0.0 0.0 28.149003871020046 0.0 27 3.377853441694872E-4 0.0 0.0 28.55367071333509 0.0 28 3.377853441694872E-4 0.0 0.0 28.942461644474168 0.0 29 3.377853441694872E-4 0.0 0.0 29.319430088567316 0.0 30 3.377853441694872E-4 0.0 0.0 29.88454496936287 0.0 31 3.377853441694872E-4 0.0 0.0 30.25408213588429 0.0 32 3.377853441694872E-4 0.0 0.0 30.611796815359774 0.0 33 3.377853441694872E-4 0.0 0.0 30.968835924146923 0.0 34 3.377853441694872E-4 0.0 0.0 31.3133769751998 0.0 35 3.377853441694872E-4 0.0 0.0 31.692034346013795 0.0 36 3.377853441694872E-4 0.0 0.0 32.036237611722505 0.0 37 3.377853441694872E-4 0.0 0.0 32.38888551103545 0.0 38 3.377853441694872E-4 0.0 0.0 32.74660019051093 0.0 39 3.377853441694872E-4 0.0 0.0 33.09857251913554 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTGG 20 7.024793E-4 45.0 2 GCCCATA 20 7.024793E-4 45.0 25 GGTACGA 20 7.024793E-4 45.0 9 ACGCCTC 20 7.024793E-4 45.0 45 CTCGTCA 20 7.024793E-4 45.0 36 GTATTCG 20 7.024793E-4 45.0 14 ATTAGCG 20 7.024793E-4 45.0 1 TTAGCGG 50 2.1827873E-11 45.0 2 CTATCAT 25 3.8837665E-5 45.0 27 GCGCGAC 20 7.024793E-4 45.0 9 ATTACGG 25 3.8837665E-5 45.0 2 ATTACCC 20 7.024793E-4 45.0 14 CCTACCG 20 7.024793E-4 45.0 1 CCAGCGG 50 2.1827873E-11 45.0 2 ATTCGGA 20 7.024793E-4 45.0 16 CGTCTAC 20 7.024793E-4 45.0 40 CGATTGG 25 3.8837665E-5 45.0 2 ATATGCG 20 7.024793E-4 45.0 1 CGTTCTA 20 7.024793E-4 45.0 27 CCTAAGG 40 6.7848305E-9 45.0 2 >>END_MODULE