FastQCFastQC Report
Sat 18 Jun 2016
SRR3548459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302734
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC61012.015300560888437No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGT58171.9214888317797143No Hit
AATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT53731.7748254242998802No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT21820.7207647637860299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19600.6474330600461131No Hit
AATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT14580.4816109191567514No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT12120.40035146366116786No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCATC10100.3336262197176399No Hit
AACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG10070.33263525074818157No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTAT9000.29729069083750087No Hit
AAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT8450.2791229263974314No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT5830.1925783030647367No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC5710.18861442718690336No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.14930599139838935No Hit
AATGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC4010.13245951891759764No Hit
AACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT3420.11297046251825035No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190.1053730337524031No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT3180.10504271076258365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG302.1601845E-645.0000041
ACAAACG351.2079727E-745.0000041
CTCGTCA302.1601845E-645.00000436
ATTACGG351.2079727E-745.0000042
TTCACTA302.1601845E-645.00000427
TCGGGCT351.2079727E-745.0000045
GCACCGT302.1601845E-645.00000424
AATGCGG351.2079727E-745.0000042
AAGCACG351.2079727E-745.0000041
CGAGGGC351.2079727E-745.0000044
TCGTCAT302.1601845E-645.00000437
TGGGACG302.1601845E-645.0000046
TACAGCG351.2079727E-745.0000041
CCGCACG302.1601845E-645.0000041
GTAGTGC302.1601845E-645.00000429
GCCCCGG207.025044E-445.02
CTATGCG207.025044E-445.01
AATCCGC207.025044E-445.035
CGAACAG207.025044E-445.01
ACACGAC750.045.026