##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302734
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59558886679395	31.0	31.0	34.0	30.0	34.0
2	31.783598802909484	31.0	31.0	34.0	30.0	34.0
3	31.937238631934306	33.0	31.0	34.0	30.0	34.0
4	35.61778326847992	37.0	35.0	37.0	33.0	37.0
5	35.36017758163933	37.0	35.0	37.0	33.0	37.0
6	35.38412599840124	37.0	35.0	37.0	33.0	37.0
7	35.35322428270363	37.0	35.0	37.0	33.0	37.0
8	35.486833325625796	37.0	35.0	37.0	33.0	37.0
9	37.24244716483778	39.0	37.0	39.0	34.0	39.0
10	36.73641546704368	39.0	37.0	39.0	32.0	39.0
11	36.73340622460642	39.0	35.0	39.0	32.0	39.0
12	36.723866496660435	39.0	35.0	39.0	32.0	39.0
13	36.7687606942068	39.0	35.0	39.0	32.0	39.0
14	37.830874629212445	39.0	37.0	41.0	33.0	41.0
15	37.94356101395945	40.0	37.0	41.0	33.0	41.0
16	37.72349653491184	39.0	37.0	41.0	32.0	41.0
17	37.66681641308872	39.0	37.0	41.0	32.0	41.0
18	37.52223073721485	39.0	36.0	41.0	32.0	41.0
19	37.46113419701784	39.0	36.0	41.0	32.0	41.0
20	37.52275595076866	39.0	36.0	41.0	32.0	41.0
21	37.56526521632853	39.0	36.0	41.0	32.0	41.0
22	37.500525213553814	39.0	36.0	41.0	32.0	41.0
23	37.3088090534925	39.0	36.0	41.0	32.0	41.0
24	37.20422218845587	39.0	36.0	40.0	31.0	41.0
25	37.26706944049892	39.0	36.0	41.0	32.0	41.0
26	37.18290974915272	39.0	36.0	41.0	32.0	41.0
27	37.16801878877166	39.0	36.0	41.0	31.0	41.0
28	37.061189030634154	39.0	36.0	41.0	31.0	41.0
29	36.87894323069097	39.0	35.0	40.0	31.0	41.0
30	36.97684435841366	39.0	36.0	40.0	31.0	41.0
31	36.8181538908745	39.0	35.0	40.0	31.0	41.0
32	36.739470954699506	39.0	35.0	40.0	30.0	41.0
33	36.72625142864693	39.0	35.0	40.0	31.0	41.0
34	36.630414819610614	39.0	35.0	40.0	30.0	41.0
35	36.530356682764406	39.0	35.0	40.0	30.0	41.0
36	36.50006276136806	39.0	35.0	40.0	30.0	41.0
37	36.39724973078676	39.0	35.0	40.0	30.0	41.0
38	36.33440247874371	39.0	35.0	40.0	30.0	41.0
39	36.264284817694744	39.0	35.0	40.0	30.0	41.0
40	36.012611731751306	39.0	35.0	40.0	29.0	41.0
41	36.0073199574544	39.0	35.0	40.0	29.0	41.0
42	35.878639333540335	38.0	35.0	40.0	28.0	41.0
43	35.86057727245701	38.0	35.0	40.0	28.0	41.0
44	35.8379072056657	38.0	35.0	40.0	28.0	41.0
45	35.53747844642491	38.0	35.0	40.0	27.0	41.0
46	35.305023552029176	38.0	34.0	40.0	26.0	41.0
47	35.21802638620043	38.0	34.0	40.0	26.0	41.0
48	35.14559646422272	38.0	34.0	40.0	26.0	41.0
49	34.98026980781808	38.0	34.0	40.0	26.0	41.0
50	34.917851975661804	38.0	34.0	40.0	26.0	41.0
51	33.421614354515846	36.0	31.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	8.0
15	7.0
16	29.0
17	70.0
18	147.0
19	347.0
20	595.0
21	992.0
22	1372.0
23	1633.0
24	1980.0
25	2339.0
26	2611.0
27	3027.0
28	3674.0
29	4651.0
30	5977.0
31	7849.0
32	10340.0
33	13422.0
34	18906.0
35	24215.0
36	28663.0
37	40357.0
38	62073.0
39	67441.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.636492762623295	40.09724708820284	16.448433278059284	24.817826871114576
2	18.386107936340153	40.22574273124261	17.52958042373833	23.858568908678908
3	18.809582009288683	30.82475044098119	26.729406013199707	23.63626153653042
4	24.34975919454042	29.596609564832494	17.762788454550858	28.290842786076226
5	16.907582233908318	38.05915424101687	18.840302047341893	26.19296147773293
6	20.129882999597008	32.55068806278779	24.769930037590758	22.549498900024446
7	74.69197381199336	12.243421617657745	8.133212655334386	4.931391915014501
8	76.40635012915628	5.599965646409059	5.2177819471879605	12.775902277246692
9	73.87508505816987	9.142019066242971	10.772823666981575	6.210072208605574
10	29.094188297317114	41.48823719833253	14.553039962475308	14.864534541875047
11	19.916494348173643	26.32740293458944	34.39686325288868	19.359239464348242
12	22.32553991292686	23.074051807857725	37.782673898537986	16.817734380677425
13	23.476715532447628	27.769923431130962	29.06974439607048	19.683616640350934
14	16.48807203683762	28.665759379521294	36.005866536299195	18.840302047341893
15	15.861119002160311	31.43353571121843	28.587803153923907	24.117542132697352
16	17.94149319204318	30.577999167586068	31.00510679342261	20.475400846948148
17	16.829956331300746	30.66091023803075	29.747567171179977	22.76156625948853
18	19.517794499461573	29.903479622374757	26.14473432121929	24.433991556944378
19	18.25166647948364	33.27046185760437	30.531093303031703	17.94677835988029
20	19.94853567818613	33.79270250450891	26.167526607516827	20.09123520978813
21	18.893153725713002	29.261992376145397	28.43717587056624	23.40767802757536
22	19.69187471509642	31.051021689007513	29.000376568208395	20.256727027687674
23	20.47242794003977	28.96701394623663	25.29349197645458	25.267066137269023
24	16.614585741938466	34.85006639492095	25.931345669795924	22.60400219334465
25	19.410109204780433	29.115989614645198	27.24603116927732	24.227870011297046
26	22.770484980213652	31.739414799791238	23.05918727331585	22.430912946679264
27	17.615464401091387	28.427596503861473	28.229733032959626	25.72720606208751
28	23.797459155562308	27.622599377671484	26.587697450567166	21.992244016199038
29	19.737459287691507	30.562473987064553	25.61919044441655	24.080876280827393
30	22.320585068079566	25.526369684277284	24.644737624449185	27.50830762319396
31	21.93873169184829	29.014580456770627	26.03209418169086	23.01459366969022
32	19.349329774653658	26.235903466409454	27.383775856032027	27.03099090290486
33	23.061829857234404	25.943897943409066	28.54056696637973	22.453705232976805
34	18.117885668606764	25.004129037372742	27.076245152510126	29.80174014151037
35	20.56227579327066	27.528457325572948	27.320353841986694	24.5889130391697
36	18.71610060316978	24.907014078365826	31.14285148017732	25.23403383828708
37	19.18383795675411	27.187894323069095	26.787873182397746	26.840394537779037
38	20.37927685691069	26.49586765939736	33.181604973342935	19.94325051034902
39	18.97144027430021	23.937516103245756	31.747012228557082	25.344031393896955
40	21.440604623200564	29.920326094855547	26.877060389649	21.762008892294883
41	18.767300666591794	24.64969246929648	31.992772532982748	24.590234331128975
42	23.96922711026842	27.492452119682625	24.938394762398673	23.59992600765028
43	20.879385863497326	24.597831759894824	28.328829929905464	26.193952446702383
44	21.78314956364333	23.07074857795953	26.748234423619415	28.397867434777723
45	25.917802427213328	24.718399651178924	28.00280113895367	21.36099678265408
46	18.523522300105043	23.92099995375478	35.10507574306156	22.450402003078608
47	21.083195148215925	26.596285848302472	27.03693671672161	25.28358228675999
48	19.751002530274103	22.53463436548257	33.60673066124056	24.107632443002768
49	21.592883521507332	23.017566576598597	29.807355632337302	25.582194269556773
50	19.5131699776041	22.13494354780104	27.940039770887974	30.411846703706885
51	18.659285048920836	21.59321384449715	33.93408074415163	25.813420362430385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2283.0
1	1800.5
2	1318.0
3	914.5
4	511.0
5	480.0
6	449.0
7	493.0
8	537.0
9	591.5
10	646.0
11	707.0
12	768.0
13	788.5
14	809.0
15	988.0
16	1167.0
17	1273.5
18	1380.0
19	1305.5
20	1231.0
21	1376.5
22	1522.0
23	1735.0
24	1948.0
25	2166.0
26	2581.0
27	2778.0
28	3234.5
29	3691.0
30	4489.0
31	5287.0
32	5828.0
33	6369.0
34	7097.5
35	7826.0
36	8739.0
37	9652.0
38	10237.5
39	10823.0
40	11599.0
41	12375.0
42	14028.5
43	15682.0
44	24994.0
45	34306.0
46	34932.0
47	35558.0
48	34687.5
49	33817.0
50	31056.5
51	28296.0
52	24706.5
53	21117.0
54	18470.5
55	15824.0
56	13453.0
57	11082.0
58	10155.0
59	9228.0
60	8494.5
61	7761.0
62	6659.5
63	5558.0
64	4487.5
65	3417.0
66	2790.5
67	2164.0
68	1714.5
69	1265.0
70	1065.5
71	866.0
72	625.5
73	385.0
74	390.5
75	268.0
76	140.0
77	94.0
78	48.0
79	37.0
80	26.0
81	23.0
82	20.0
83	14.5
84	9.0
85	7.0
86	5.0
87	5.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.098145566735155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51924422673197	24.882239854129367
2	9.55316346272589	6.132776628987824
3	3.2416745564565925	3.1215522537937597
4	1.692874490593998	2.173525273011951
5	1.0507141975054541	1.686298863028269
6	0.7286049479273865	1.4032120607530043
7	0.5464537109455399	1.2278105531588788
8	0.42707775902523365	1.0966723262005589
9	0.37356440126785495	1.0791652077401284
>10	4.15140163833203	30.35635244141722
>50	0.6184909233112419	13.096645900361374
>100	0.08335734573745524	4.158105795847179
>500	0.004116412135182974	0.9576063474865724
>1k	0.006174618202774462	2.916421677115884
>5k	0.003087309101387231	5.711614816968031
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC	6101	2.015300560888437	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGT	5817	1.9214888317797143	No Hit
AATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT	5373	1.7748254242998802	No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT	2182	0.7207647637860299	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1960	0.6474330600461131	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT	1458	0.4816109191567514	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT	1212	0.40035146366116786	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCATC	1010	0.3336262197176399	No Hit
AACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTG	1007	0.33263525074818157	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTAT	900	0.29729069083750087	No Hit
AAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT	845	0.2791229263974314	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTT	583	0.1925783030647367	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	571	0.18861442718690336	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	452	0.14930599139838935	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	401	0.13245951891759764	No Hit
AACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT	342	0.11297046251825035	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	319	0.1053730337524031	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT	318	0.10504271076258365	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3032298981944546E-4	0.0	0.0	0.8452965309479609	0.0
2	3.3032298981944546E-4	0.0	0.0	1.2770286786419762	0.0
3	3.3032298981944546E-4	0.0	0.0	2.1256284394881315	0.0
4	3.3032298981944546E-4	0.0	0.0	6.079264304637074	0.0
5	3.3032298981944546E-4	0.0	0.0	6.550304888119603	0.0
6	3.3032298981944546E-4	0.0	0.0	8.606235176755831	0.0
7	3.3032298981944546E-4	0.0	0.0	10.159083551897044	0.0
8	3.3032298981944546E-4	0.0	0.0	11.03774270481677	0.0
9	3.3032298981944546E-4	0.0	0.0	14.351542938685446	0.0
10	3.3032298981944546E-4	0.0	0.0	16.314652467182412	0.0
11	6.606459796388909E-4	0.0	0.0	20.076040352256435	0.0
12	6.606459796388909E-4	0.0	0.0	21.048511234284884	0.0
13	6.606459796388909E-4	0.0	0.0	21.53937119715658	0.0
14	6.606459796388909E-4	0.0	0.0	22.19968685380565	0.0
15	6.606459796388909E-4	0.0	0.0	22.6115996221105	0.0
16	6.606459796388909E-4	0.0	0.0	23.27885206154578	0.0
17	6.606459796388909E-4	0.0	0.0	24.127451822391937	0.0
18	6.606459796388909E-4	0.0	0.0	25.64924983649012	0.0
19	6.606459796388909E-4	0.0	0.0	26.17941823515033	0.0
20	6.606459796388909E-4	0.0	0.0	26.771357032906778	0.0
21	6.606459796388909E-4	0.0	0.0	27.36990229045961	0.0
22	6.606459796388909E-4	0.0	0.0	27.976705622757933	0.0
23	6.606459796388909E-4	0.0	0.0	28.498615946672658	0.0
24	6.606459796388909E-4	0.0	0.0	28.87848738496502	0.0
25	6.606459796388909E-4	0.0	0.0	29.226647816234713	0.0
26	6.606459796388909E-4	0.0	0.0	29.563577265850547	0.0
27	6.606459796388909E-4	0.0	0.0	29.98044487900269	0.0
28	6.606459796388909E-4	0.0	0.0	30.3679137460609	0.0
29	6.606459796388909E-4	0.0	0.0	30.722680637126984	0.0
30	6.606459796388909E-4	0.0	0.0	31.274650353115277	0.0
31	6.606459796388909E-4	0.0	0.0	31.64956694656035	0.0
32	6.606459796388909E-4	0.0	0.0	32.020189341137765	0.0
33	6.606459796388909E-4	0.0	0.0	32.38519624488825	0.0
34	6.606459796388909E-4	0.0	0.0	32.73236570718849	0.0
35	6.606459796388909E-4	0.0	0.0	33.11388876042995	0.0
36	6.606459796388909E-4	0.0	0.0	33.47228920438405	0.0
37	6.606459796388909E-4	0.0	0.0	33.81384317585735	0.0
38	6.606459796388909E-4	0.0	0.0	34.169931358882714	0.0
39	6.606459796388909E-4	0.0	0.0	34.50851242344765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	30	2.1601845E-6	45.000004	1
ACAAACG	35	1.2079727E-7	45.000004	1
CTCGTCA	30	2.1601845E-6	45.000004	36
ATTACGG	35	1.2079727E-7	45.000004	2
TTCACTA	30	2.1601845E-6	45.000004	27
TCGGGCT	35	1.2079727E-7	45.000004	5
GCACCGT	30	2.1601845E-6	45.000004	24
AATGCGG	35	1.2079727E-7	45.000004	2
AAGCACG	35	1.2079727E-7	45.000004	1
CGAGGGC	35	1.2079727E-7	45.000004	4
TCGTCAT	30	2.1601845E-6	45.000004	37
TGGGACG	30	2.1601845E-6	45.000004	6
TACAGCG	35	1.2079727E-7	45.000004	1
CCGCACG	30	2.1601845E-6	45.000004	1
GTAGTGC	30	2.1601845E-6	45.000004	29
GCCCCGG	20	7.025044E-4	45.0	2
CTATGCG	20	7.025044E-4	45.0	1
AATCCGC	20	7.025044E-4	45.0	35
CGAACAG	20	7.025044E-4	45.0	1
ACACGAC	75	0.0	45.0	26
>>END_MODULE