##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272154
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65027153743836	31.0	31.0	34.0	30.0	34.0
2	31.858164862541063	33.0	31.0	34.0	30.0	34.0
3	32.04412207794117	33.0	31.0	34.0	30.0	34.0
4	35.72265702506669	37.0	35.0	37.0	33.0	37.0
5	35.480985030534185	37.0	35.0	37.0	33.0	37.0
6	34.86755660398157	37.0	35.0	37.0	32.0	37.0
7	35.27520080542634	37.0	35.0	37.0	33.0	37.0
8	35.66683201422724	37.0	35.0	37.0	33.0	37.0
9	37.343897205258784	39.0	37.0	39.0	34.0	39.0
10	36.88210718931193	39.0	37.0	39.0	33.0	39.0
11	36.930043284316966	39.0	37.0	39.0	33.0	39.0
12	36.97311081226071	39.0	37.0	39.0	33.0	39.0
13	36.909551209976705	39.0	37.0	39.0	33.0	39.0
14	38.035623213327746	40.0	37.0	41.0	33.0	41.0
15	38.087211652226316	40.0	37.0	41.0	33.0	41.0
16	37.92808850871198	40.0	37.0	41.0	33.0	41.0
17	37.9031467477972	39.0	37.0	41.0	33.0	41.0
18	37.84174401258111	39.0	37.0	41.0	33.0	41.0
19	37.80719372120197	39.0	36.0	41.0	33.0	41.0
20	37.74666916525203	39.0	36.0	41.0	33.0	41.0
21	37.74198799209271	39.0	36.0	41.0	33.0	41.0
22	37.72700382871462	39.0	36.0	41.0	33.0	41.0
23	37.60269553267635	39.0	36.0	41.0	33.0	41.0
24	37.48815376588255	39.0	36.0	41.0	32.0	41.0
25	37.52410399994121	39.0	36.0	41.0	32.0	41.0
26	37.47375383055182	39.0	36.0	41.0	32.0	41.0
27	37.42840450627219	39.0	36.0	41.0	32.0	41.0
28	37.35834490766258	39.0	36.0	41.0	32.0	41.0
29	37.16583258008333	39.0	36.0	41.0	31.0	41.0
30	37.1908368056321	39.0	36.0	41.0	32.0	41.0
31	37.11914945214841	39.0	36.0	41.0	31.0	41.0
32	37.041601446239994	39.0	36.0	41.0	31.0	41.0
33	36.81808828824857	39.0	36.0	41.0	31.0	41.0
34	36.86200827472681	39.0	36.0	41.0	31.0	41.0
35	36.74851370914997	39.0	35.0	41.0	30.0	41.0
36	36.74349816647927	39.0	35.0	41.0	31.0	41.0
37	36.627611572859486	39.0	35.0	40.0	30.0	41.0
38	36.47211872689727	39.0	35.0	40.0	30.0	41.0
39	36.45749832815244	39.0	35.0	40.0	30.0	41.0
40	36.36779911373708	39.0	35.0	40.0	30.0	41.0
41	36.380332458828455	39.0	35.0	40.0	30.0	41.0
42	36.28161996516678	39.0	35.0	40.0	30.0	41.0
43	36.22696708481227	39.0	35.0	40.0	30.0	41.0
44	36.191623125142385	39.0	35.0	40.0	30.0	41.0
45	35.96632053910653	39.0	35.0	40.0	29.0	41.0
46	35.80214510901916	38.0	35.0	40.0	29.0	41.0
47	35.70053719585235	38.0	35.0	40.0	28.0	41.0
48	35.61919721922147	38.0	35.0	40.0	28.0	41.0
49	35.51584397069306	38.0	35.0	40.0	27.0	41.0
50	35.435551195279146	38.0	35.0	40.0	27.0	41.0
51	34.14176532404448	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	1.0
13	2.0
14	2.0
15	6.0
16	21.0
17	53.0
18	153.0
19	265.0
20	520.0
21	799.0
22	1110.0
23	1386.0
24	1662.0
25	1898.0
26	1979.0
27	2339.0
28	2764.0
29	3514.0
30	4612.0
31	5822.0
32	8064.0
33	10766.0
34	15681.0
35	21552.0
36	26041.0
37	37759.0
38	58496.0
39	64872.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.21194617753184	35.540539547462096	19.879186049075155	27.368328225930906
2	20.300638609022833	34.70241113487217	20.993261168309118	24.00368908779588
3	20.25581104815656	33.03460540723267	21.911123848997256	24.798459695613513
4	20.23229495065294	32.725589188474174	19.29826495293106	27.743850907941827
5	17.486423128081896	36.39446783806227	18.676558125179128	27.442550908676704
6	19.118954709465964	38.592488076603686	19.982803853700478	22.30575336022987
7	79.25439273352588	8.310368394364955	7.057768763273735	5.377470108835439
8	81.28339102126002	6.292393277335625	5.160681084973948	7.2635346164304035
9	74.08966981929349	11.092984119285404	10.184307414184616	4.633038647236491
10	26.50153956950844	46.186717814178735	12.61454911557427	14.697193500738553
11	18.055586175474183	28.340939321119663	35.87931832712361	17.724156176282545
12	23.211490553142706	23.855243722304284	35.05588747547345	17.877378249079566
13	23.6873240885675	27.046084202326625	28.649955539878157	20.616636169227718
14	17.408526055101156	30.78551114442558	31.988506507345104	19.817456293128156
15	15.788119961492391	34.11561101435217	29.202583831213207	20.893685192942232
16	18.29405410172182	32.26555553105962	28.629011515539	20.811378851679564
17	17.389419225879465	31.932288336750513	29.30987602607347	21.368416411296547
18	18.84741727110386	29.075817368107764	27.845998956473174	24.230766404315204
19	18.805161783402045	34.070783453485895	28.80721944193361	18.316835321178452
20	20.355019584499953	31.675815898351665	28.39789236976124	19.57127214738714
21	19.870734951534793	31.225335655547966	26.983252129309143	21.920677263608106
22	18.168389955686852	30.169683341049552	28.694048222697443	22.96787848056615
23	19.3081858065654	30.17960419468389	26.589724935147014	23.922485063603695
24	15.666865083739353	33.26498967496344	27.703800054380977	23.36434518691623
25	18.79413861269722	31.194470777574463	26.85722054425068	23.154170065477633
26	20.62949653505001	31.990343702462575	25.846395790618548	21.53376397186887
27	17.827773980907867	30.253091999382704	26.794021032209702	25.125112987499726
28	19.541509586484125	27.974970053719584	31.43734797210403	21.046172387692263
29	20.558948242539152	29.75374236645429	28.16089419960758	21.526415191398986
30	22.330004335780476	27.400295420974892	27.809255054123767	22.460445189120865
31	19.879186049075155	29.481102611021704	28.590062979048625	22.049648360854516
32	24.248403477442917	27.591363713191797	25.793484571235403	22.366748238129883
33	20.5497622669518	26.90902944656334	28.244302857940724	24.296905428544132
34	19.580090683951	26.171946765434278	29.23822541649213	25.009737134122595
35	20.242215804287277	27.79198542001955	29.49837224512592	22.467426530567252
36	20.593854949771085	26.721268105557883	31.060722973022624	21.624153971648404
37	21.89863092219846	29.209932611683094	26.037831521858944	22.8536049442595
38	23.09280774855413	27.068130543736267	28.864907368622177	20.974154339087427
39	21.858947507661103	25.074038963234052	27.757446151811106	25.30956737729374
40	22.479919457366048	28.135173467962993	24.682348964189394	24.702558110481565
41	18.23158946772783	25.800833351705283	31.360920655217267	24.606656525349617
42	22.926357870911325	27.2948404212321	27.65640042035024	22.12240128750634
43	18.957648978152076	25.704564327549846	31.24701455793411	24.09077213636397
44	23.46759555251806	23.98017299029226	27.075846763229645	25.476384693960036
45	24.222315306774842	24.5890194522219	28.641871881361286	22.546793359641967
46	19.923646170917937	24.597470549762267	31.72468528847638	23.75419799084342
47	19.139898733805126	26.255355423767423	28.076383224203944	26.528362618223504
48	20.307252511445725	23.828420673589218	32.62123650580186	23.243090309163193
49	21.57748921566466	24.27485908713449	29.32567590408372	24.821975793117133
50	19.713838488502834	22.8473584808601	28.389073833197383	29.04972919743968
51	19.185093733694895	22.63975543258596	33.235594553083914	24.939556280635227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2363.0
1	1901.0
2	1439.0
3	995.5
4	552.0
5	542.5
6	533.0
7	596.0
8	659.0
9	760.0
10	861.0
11	916.5
12	972.0
13	1007.0
14	1042.0
15	1073.0
16	1104.0
17	1118.5
18	1133.0
19	1273.5
20	1414.0
21	1303.5
22	1193.0
23	1448.0
24	1703.0
25	1832.5
26	2586.0
27	3210.0
28	3620.0
29	4030.0
30	4934.0
31	5838.0
32	6163.0
33	6488.0
34	7360.5
35	8233.0
36	8801.5
37	9370.0
38	9749.0
39	10128.0
40	10866.5
41	11605.0
42	13778.0
43	15951.0
44	19886.5
45	23822.0
46	26048.5
47	28275.0
48	30364.5
49	32454.0
50	28878.0
51	25302.0
52	22701.5
53	20101.0
54	17178.5
55	14256.0
56	12482.0
57	10708.0
58	9097.5
59	7487.0
60	6724.5
61	5962.0
62	5015.5
63	4069.0
64	3524.5
65	2980.0
66	2395.5
67	1811.0
68	1531.0
69	1251.0
70	976.5
71	702.0
72	588.5
73	475.0
74	393.0
75	245.0
76	179.0
77	132.0
78	85.0
79	64.5
80	44.0
81	41.0
82	38.0
83	41.0
84	44.0
85	26.0
86	8.0
87	6.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	272154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.06009554340242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12098026724195	28.210508015614366
2	10.406997302205527	7.71368628679336
3	3.6170247318540376	4.021418464360805
4	1.8359997743576426	2.7216930822143803
5	1.1669997005356192	2.1624560200486034
6	0.8069997908429074	1.7944493611286347
7	0.6299998366864428	1.6343497897949242
8	0.46499987945860094	1.3786351968325086
9	0.39799989682692427	1.3274922782403105
>10	4.136998927570314	32.406080344469345
>50	0.34299991108451	8.148401094826827
>100	0.06299998366858346	4.058820611749827
>500	0.003999998963084664	0.9680094446576467
>1k	0.004999998703855831	3.4540000092684786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	3035	1.1151774363044453	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2013	0.739654754293525	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC	1489	0.5471167059826422	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGT	1440	0.5291121938314337	No Hit
AATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT	1343	0.49347060855251074	No Hit
AAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT	851	0.31269060899343754	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT	650	0.23883536527113328	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	601	0.22083085311992473	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	510	0.1873939019819661	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	394	0.14477097525665616	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCT	362	0.13301292650484653	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	330	0.12125487775303688	No Hit
AACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTG	314	0.11537585337713208	No Hit
AATGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTT	312	0.11464097533014396	No Hit
AACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT	299	0.10986426802472128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2033628019430176	0.0
2	0.0	0.0	0.0	1.6505360935352777	0.0
3	0.0	0.0	0.0	2.263424384723355	0.0
4	0.0	0.0	0.0	5.129448767976954	0.0
5	0.0	0.0	0.0	5.54355254745475	0.0
6	0.0	0.0	0.0	7.621787664337103	0.0
7	0.0	0.0	0.0	9.214635831183815	0.0
8	0.0	0.0	0.0	10.383459364918393	0.0
9	0.0	0.0	0.0	12.30075618951035	0.0
10	0.0	0.0	0.0	14.513106549968033	0.0
11	0.0	0.0	0.0	16.94996215378058	0.0
12	0.0	0.0	0.0	18.093064955870574	0.0
13	0.0	0.0	0.0	18.5545683693791	0.0
14	0.0	0.0	0.0	19.093601416844873	0.0
15	0.0	0.0	0.0	19.513951659722068	0.0
16	0.0	0.0	0.0	20.341424340630674	0.0
17	0.0	0.0	0.0	21.387523240518235	0.0
18	0.0	0.0	0.0	22.959794822049282	0.0
19	0.0	0.0	0.0	23.582236527848202	0.0
20	3.6743902349405116E-4	0.0	0.0	24.289924087097745	0.0
21	3.6743902349405116E-4	0.0	0.0	24.96784908544427	0.0
22	3.6743902349405116E-4	0.0	0.0	25.760415059120938	0.0
23	3.6743902349405116E-4	0.0	0.0	26.489414081733138	0.0
24	3.6743902349405116E-4	0.0	0.0	27.01485188532963	0.0
25	3.6743902349405116E-4	0.0	0.0	27.45871822571044	0.0
26	3.6743902349405116E-4	0.0	0.0	27.900379931950294	0.0
27	3.6743902349405116E-4	0.0	0.0	28.458519808637757	0.0
28	3.6743902349405116E-4	0.0	0.0	28.968892612270995	0.0
29	3.6743902349405116E-4	0.0	0.0	29.486981635397605	0.0
30	3.6743902349405116E-4	0.0	0.0	30.25897102375861	0.0
31	3.6743902349405116E-4	0.0	0.0	30.744725412817743	0.0
32	3.6743902349405116E-4	0.0	0.0	31.211005533631695	0.0
33	3.6743902349405116E-4	0.0	0.0	31.668099678858294	0.0
34	3.6743902349405116E-4	0.0	0.0	32.11380321435658	0.0
35	3.6743902349405116E-4	0.0	0.0	32.59331114001631	0.0
36	3.6743902349405116E-4	0.0	0.0	33.05995869985376	0.0
37	3.6743902349405116E-4	0.0	0.0	33.532117845043615	0.0
38	3.6743902349405116E-4	0.0	0.0	33.98774223417624	0.0
39	3.6743902349405116E-4	0.0	0.0	34.43675272088597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCA	35	1.2073542E-7	45.000004	36
TGCGGGC	35	1.2073542E-7	45.000004	4
ACGGGCG	35	1.2073542E-7	45.000004	5
CGTAGTG	35	1.2073542E-7	45.000004	28
CTAAACG	35	1.2073542E-7	45.000004	1
ACATCCG	45	3.8198777E-10	45.000004	1
CCACACG	35	1.2073542E-7	45.000004	1
GCGAGAC	25	3.882936E-5	45.0	21
CTGCTAC	20	7.023789E-4	45.0	41
TTCTACG	20	7.023789E-4	45.0	1
TTAGGCG	20	7.023789E-4	45.0	1
CGGAAAG	25	3.882936E-5	45.0	1
GTACCGG	40	6.7811925E-9	45.0	2
TCTTGCG	25	3.882936E-5	45.0	1
ACACGAC	25	3.882936E-5	45.0	26
CTCGTCC	20	7.023789E-4	45.0	37
CTCGTAG	25	3.882936E-5	45.0	1
TGCCACG	20	7.023789E-4	45.0	1
GCGGTCG	20	7.023789E-4	45.0	12
ACGCACA	20	7.023789E-4	45.0	37
>>END_MODULE