SRR3548452_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449404
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	3204	0.7129442550578099	RNA PCR Primer, Index 27 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2215	0.4928750077880927	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGT	1765	0.3927423876957037	No Hit
AATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1667	0.3709357282089167	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	1632	0.36314763553506424	No Hit
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1277	0.2841541241288462	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	1024	0.22785733994356971	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	641	0.1426333543982697	RNA PCR Primer, Index 27 (95% over 24bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	585	0.13017240612010575	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	526	0.11704390704132585	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	454	0.1010226878265436	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTAGG	65	0.0	45.000004	2
GTTGACG	20	7.0286973E-4	45.0	29
TTTAGCG	35	1.2097189E-7	45.0	1
AACGTAT	20	7.0286973E-4	45.0	10
AACGTAG	20	7.0286973E-4	45.0	1
GAAATCG	40	6.7993824E-9	45.0	23
AAGTACG	25	3.8870017E-5	45.0	1
AGCCGTA	20	7.0286973E-4	45.0	27
TTAGGCA	25	3.8870017E-5	45.0	13
TCGTTGA	75	0.0	45.0	24
AAATCGC	40	6.7993824E-9	45.0	24
GCGACAC	20	7.0286973E-4	45.0	37
ATGATCG	20	7.0286973E-4	45.0	27
CTCGTCA	45	3.8380676E-10	45.0	36
GTCGAGT	35	1.2097189E-7	45.0	14
GTCGAGC	20	7.0286973E-4	45.0	12
AGTCACG	20	7.0286973E-4	45.0	32
AGTCAAT	20	7.0286973E-4	45.0	41
TCACATT	20	7.0286973E-4	45.0	37
CCGGCAT	20	7.0286973E-4	45.0	40