##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	449404
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64376151525131	31.0	31.0	34.0	30.0	34.0
2	31.85983213322534	33.0	31.0	34.0	30.0	34.0
3	32.031285880855535	33.0	31.0	34.0	30.0	34.0
4	35.71551877597885	37.0	35.0	37.0	33.0	37.0
5	35.45951526911198	37.0	35.0	37.0	33.0	37.0
6	34.81946311114276	37.0	35.0	37.0	32.0	37.0
7	35.213240202579414	37.0	35.0	37.0	33.0	37.0
8	35.60562656318146	37.0	35.0	37.0	33.0	37.0
9	37.28323512919333	39.0	37.0	39.0	34.0	39.0
10	36.83470997142883	39.0	37.0	39.0	32.0	39.0
11	36.79092753958576	39.0	37.0	39.0	32.0	39.0
12	36.53380254737385	39.0	35.0	39.0	32.0	39.0
13	36.46281074489769	39.0	35.0	39.0	32.0	39.0
14	37.55848412564196	39.0	36.0	41.0	33.0	41.0
15	37.56272974873388	39.0	36.0	41.0	33.0	41.0
16	37.475583216882804	39.0	36.0	41.0	32.0	41.0
17	37.44810460076012	39.0	36.0	41.0	32.0	41.0
18	37.37210616727933	39.0	36.0	41.0	32.0	41.0
19	37.34482559122749	39.0	35.0	41.0	32.0	41.0
20	37.31892016982492	39.0	35.0	41.0	32.0	41.0
21	37.27764327865351	39.0	35.0	41.0	32.0	41.0
22	37.29878906284768	39.0	35.0	41.0	32.0	41.0
23	37.20035869729686	39.0	35.0	41.0	32.0	41.0
24	37.068931295671604	39.0	35.0	41.0	32.0	41.0
25	37.10063995870086	39.0	35.0	41.0	32.0	41.0
26	37.057011063541935	39.0	35.0	41.0	32.0	41.0
27	36.98333570684729	39.0	35.0	40.0	31.0	41.0
28	36.91124022038077	39.0	35.0	40.0	31.0	41.0
29	36.663794714777794	39.0	35.0	40.0	31.0	41.0
30	36.69642237274257	39.0	35.0	40.0	31.0	41.0
31	36.63097569225018	39.0	35.0	40.0	31.0	41.0
32	36.565324296178936	39.0	35.0	40.0	31.0	41.0
33	36.315524561419124	39.0	35.0	40.0	30.0	41.0
34	36.29866667853424	39.0	35.0	40.0	30.0	41.0
35	36.185837242214134	38.0	35.0	40.0	30.0	41.0
36	36.17543012523253	38.0	35.0	40.0	30.0	41.0
37	36.14144511397318	38.0	35.0	40.0	30.0	41.0
38	35.97006924726972	38.0	35.0	40.0	29.0	41.0
39	35.91383476782583	38.0	35.0	40.0	29.0	41.0
40	35.823437263575755	38.0	35.0	40.0	29.0	41.0
41	35.828268106202884	38.0	35.0	40.0	29.0	41.0
42	35.76275689579977	38.0	35.0	40.0	29.0	41.0
43	35.689680109656344	38.0	35.0	40.0	29.0	41.0
44	35.60484107840607	38.0	34.0	40.0	29.0	41.0
45	35.40734839921318	38.0	34.0	40.0	28.0	41.0
46	35.22036964512999	37.0	34.0	40.0	27.0	41.0
47	35.07289654742726	37.0	34.0	40.0	27.0	41.0
48	35.03393605753398	37.0	34.0	40.0	27.0	41.0
49	34.90266441776219	37.0	34.0	40.0	26.0	41.0
50	34.84510818773309	37.0	34.0	40.0	26.0	41.0
51	33.60868394584828	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	4.0
13	5.0
14	12.0
15	34.0
16	60.0
17	129.0
18	254.0
19	456.0
20	821.0
21	1289.0
22	1828.0
23	2364.0
24	2865.0
25	3308.0
26	3830.0
27	4567.0
28	5740.0
29	7188.0
30	9313.0
31	12190.0
32	15831.0
33	22411.0
34	35909.0
35	40333.0
36	42128.0
37	59459.0
38	85220.0
39	91841.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.680510186825217	33.424936137640074	20.320468887682352	26.574084787852353
2	24.271702076528022	32.56579825724737	18.494494931064253	24.668004735160345
3	20.803998184261822	31.602967485825673	20.27819067031001	27.314843659602495
4	21.49469074596577	31.771412804514426	18.584614289147407	28.1492821603724
5	18.266637591120684	34.968758622531176	18.1947646215877	28.569839164760435
6	20.262614484962306	38.62382177283691	19.052567400379168	22.06099634182161
7	79.93075273028278	7.494147804647934	7.178173759023061	5.39692570604623
8	81.8904593639576	6.015077747416578	5.27899173127075	6.815471157355074
9	75.19536986764693	8.788751323975754	9.998575891625352	6.017302916751965
10	38.736637858141	28.584970316241066	14.54526439462043	18.133127430997497
11	29.178422977988628	27.95836263139625	23.61371950405426	19.249494886560868
12	29.655944317362554	23.297077907628772	28.292138031704212	18.754839743304466
13	27.670870753264325	24.60525495990245	26.24698489555055	21.47688939128268
14	21.441064164982958	26.52602113020801	29.166407063577537	22.866507641231497
15	19.99292396151347	28.77878256535322	28.935211969630885	22.293081503502414
16	23.000017801354684	26.80483484793193	27.354896707639455	22.840250643073936
17	22.00692472697172	28.180434531067817	27.465710140541695	22.34693060141877
18	23.396765493854083	26.41921300210946	26.084770051000884	24.099251453035574
19	23.15978495963543	31.17373232102963	25.823980204893594	19.84250251444135
20	25.104137924896087	29.339302720937066	24.16956680403379	21.386992550133066
21	24.69982465665637	29.8940374362489	24.542060150777477	20.864077756317258
22	22.989114471611288	28.445452198912335	24.67423520929943	23.891198120176945
23	22.50224742102874	28.28234728662851	24.652651066746177	24.562754225596567
24	21.5743518081726	28.72871625530703	25.425674893859423	24.271257042660945
25	23.949942590631146	27.3039403298591	23.076786143425515	25.669330936084233
26	24.816201012897082	28.378919635784282	22.280620555224253	24.524258796094383
27	20.71276624151098	26.17711457841942	24.899199829106994	28.21091935096261
28	23.596140666304706	25.78503974152433	25.61570435510142	25.00311523706954
29	23.216526777687783	28.87157212663884	22.32223122179598	25.5896698738774
30	25.105918060364395	27.488184350829098	21.95129549358706	25.454602095219446
31	24.417895701862914	26.648182926720725	21.97599487320985	26.957926498206515
32	23.793290669419942	24.78660626073644	25.78503974152433	25.635063328319283
33	25.018913939350785	26.52757874874278	23.183149237656984	25.27035807424945
34	23.008918478696227	24.692259080916056	26.6780001958149	25.62082224457281
35	22.424366494290215	25.083221333143452	27.509100942581732	24.9833112299846
36	22.481553346209644	27.204252743633788	27.096999581668165	23.217194328488397
37	21.545424606812578	28.388710380859983	24.545620421714094	25.520244590613345
38	23.210741337415776	26.949693371665585	26.742752623474647	23.09681266744399
39	22.307990138049504	24.968402595437514	27.903845982679282	24.8197612838337
40	23.9773121734564	25.332885332573806	26.62081334389547	24.068989150074323
41	21.38966275333553	22.960632304118345	29.755631903587865	25.894073038958265
42	23.76948135753131	25.228524890744186	25.777696682717554	25.22429706900695
43	21.033190625806625	24.303299481090512	27.168872551201144	27.49463734190172
44	22.224991321839592	21.613069754608325	25.749437032158145	30.412501891393934
45	23.968411496114854	22.34025509341261	27.251203816610442	26.440129593862093
46	20.465772445283086	23.50557627435448	30.067823161342577	25.960828119019858
47	21.69562353695116	26.004663954926972	26.19358083150128	26.106131676620592
48	20.617974027823518	22.778390935550195	31.301234523947276	25.302400512679014
49	21.328915630479482	22.078797696504704	30.197995567462684	26.39429110555313
50	21.265498304420966	22.30242721471104	28.130145704088083	28.30192877677991
51	21.475554289681444	21.418144920828475	30.694208329253858	26.412092460236224
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2618.0
1	2065.5
2	1513.0
3	1042.5
4	572.0
5	568.0
6	564.0
7	594.5
8	625.0
9	658.0
10	691.0
11	777.5
12	864.0
13	891.5
14	919.0
15	1073.5
16	1228.0
17	1257.5
18	1287.0
19	1358.5
20	1430.0
21	1327.5
22	1225.0
23	1483.0
24	1741.0
25	2298.0
26	2685.5
27	2516.0
28	3651.0
29	4786.0
30	4808.5
31	4831.0
32	5953.0
33	7075.0
34	8081.0
35	9087.0
36	9587.0
37	10087.0
38	11686.0
39	13285.0
40	13905.0
41	14525.0
42	17620.5
43	20716.0
44	25137.0
45	29558.0
46	33282.0
47	37006.0
48	39850.5
49	42695.0
50	41988.0
51	41281.0
52	36583.5
53	31886.0
54	29146.5
55	26407.0
56	24710.0
57	23013.0
58	22957.0
59	22901.0
60	22968.0
61	23035.0
62	21764.5
63	20494.0
64	17706.0
65	14918.0
66	12679.0
67	10440.0
68	8808.5
69	7177.0
70	5840.5
71	4504.0
72	3930.5
73	3357.0
74	2522.0
75	1547.0
76	1407.0
77	1400.0
78	1393.0
79	1032.0
80	671.0
81	414.0
82	157.0
83	210.0
84	263.0
85	165.5
86	68.0
87	42.5
88	17.0
89	13.5
90	10.0
91	9.0
92	8.0
93	8.5
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	449404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.55739454171964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2553134090914	21.776530709259067
2	10.142158100644155	5.79267220769186
3	3.7243552315713995	3.1907364528450617
4	1.7475734664613853	1.9962457988951372
5	1.060050465774257	1.5136139692624564
6	0.6957394326971553	1.1921103286658912
7	0.47941347856082495	0.958355989911581
8	0.37878628951958637	0.8653719613443898
9	0.31433898153738354	0.8079032084044846
>10	3.7956149395825083	26.306027418934192
>50	1.1889781215079431	23.744767290148694
>100	0.20981966850140174	8.593555944707475
>500	0.0023575243651842892	0.3931765600795837
>1k	0.005500890185430009	2.8689321598501127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	3204	0.7129442550578099	RNA PCR Primer, Index 27 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2215	0.4928750077880927	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGT	1765	0.3927423876957037	No Hit
AATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1667	0.3709357282089167	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	1632	0.36314763553506424	No Hit
AAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1277	0.2841541241288462	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	1024	0.22785733994356971	No Hit
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	641	0.1426333543982697	RNA PCR Primer, Index 27 (95% over 24bp)
AAAAAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	585	0.13017240612010575	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	526	0.11704390704132585	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	454	0.1010226878265436	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.675508006159269E-4	0.0	0.0	0.8070689179446556	0.0
2	6.675508006159269E-4	0.0	0.0	1.1372840473160009	0.0
3	6.675508006159269E-4	0.0	0.0	1.5990066844086834	0.0
4	6.675508006159269E-4	0.0	0.0	3.712027485291631	0.0
5	6.675508006159269E-4	0.0	0.0	4.047137987200826	0.0
6	6.675508006159269E-4	0.0	0.0	5.677742076171997	0.0
7	6.675508006159269E-4	0.0	0.0	6.95877206255396	0.0
8	6.675508006159269E-4	0.0	0.0	7.81101191800696	0.0
9	6.675508006159269E-4	0.0	0.0	9.247803757865974	0.0
10	6.675508006159269E-4	0.0	0.0	10.926916538348568	0.0
11	6.675508006159269E-4	0.0	0.0	12.739984512821426	0.0
12	6.675508006159269E-4	0.0	0.0	13.51300833993467	0.0
13	6.675508006159269E-4	0.0	0.0	13.861247340922644	0.0
14	6.675508006159269E-4	0.0	0.0	14.249094356080498	0.0
15	6.675508006159269E-4	0.0	0.0	14.56173064770229	0.0
16	6.675508006159269E-4	0.0	0.0	15.216375466172975	0.0
17	6.675508006159269E-4	0.0	0.0	15.940668084841256	0.0
18	6.675508006159269E-4	0.0	0.0	17.0643785992114	0.0
19	6.675508006159269E-4	0.0	0.0	17.469581935185268	0.0
20	6.675508006159269E-4	0.0	0.0	17.948215859226888	0.0
21	6.675508006159269E-4	0.0	0.0	18.423734546198965	0.0
22	8.900677341545692E-4	0.0	0.0	18.888349903427653	0.0
23	8.900677341545692E-4	0.0	0.0	19.38701035148775	0.0
24	8.900677341545692E-4	0.0	0.0	19.75883614743082	0.0
25	8.900677341545692E-4	0.0	0.0	20.12598908776958	0.0
26	8.900677341545692E-4	0.0	0.0	20.413258448967966	0.0
27	8.900677341545692E-4	0.0	0.0	20.757937179019322	0.0
28	8.900677341545692E-4	0.0	0.0	21.08459203745405	0.0
29	8.900677341545692E-4	0.0	0.0	21.422595259499246	0.0
30	8.900677341545692E-4	0.0	0.0	21.89433115860117	0.0
31	8.900677341545692E-4	0.0	0.0	22.231221795978673	0.0
32	8.900677341545692E-4	0.0	0.0	22.569447534957412	0.0
33	8.900677341545692E-4	0.0	0.0	22.91991170528077	0.0
34	0.0011125846676932115	0.0	0.0	23.235440717038568	0.0
35	0.0011125846676932115	0.0	0.0	23.588130056697313	0.0
36	0.0011125846676932115	0.0	0.0	23.903214034588032	0.0
37	0.0011125846676932115	0.0	0.0	24.21473774154213	0.0
38	0.0011125846676932115	0.0	0.0	24.605477476835986	0.0
39	0.0011125846676932115	0.0	0.0	25.02136162561971	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTAGG	65	0.0	45.000004	2
GTTGACG	20	7.0286973E-4	45.0	29
TTTAGCG	35	1.2097189E-7	45.0	1
AACGTAT	20	7.0286973E-4	45.0	10
AACGTAG	20	7.0286973E-4	45.0	1
GAAATCG	40	6.7993824E-9	45.0	23
AAGTACG	25	3.8870017E-5	45.0	1
AGCCGTA	20	7.0286973E-4	45.0	27
TTAGGCA	25	3.8870017E-5	45.0	13
TCGTTGA	75	0.0	45.0	24
AAATCGC	40	6.7993824E-9	45.0	24
GCGACAC	20	7.0286973E-4	45.0	37
ATGATCG	20	7.0286973E-4	45.0	27
CTCGTCA	45	3.8380676E-10	45.0	36
GTCGAGT	35	1.2097189E-7	45.0	14
GTCGAGC	20	7.0286973E-4	45.0	12
AGTCACG	20	7.0286973E-4	45.0	32
AGTCAAT	20	7.0286973E-4	45.0	41
TCACATT	20	7.0286973E-4	45.0	37
CCGGCAT	20	7.0286973E-4	45.0	40
>>END_MODULE