SRR3548450_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702572
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	5506	0.7836919205433749	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	5378	0.7654731472361551	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	4991	0.7103898248151079	RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	3725	0.5301947700733876	Illumina PCR Primer Index 7 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1848	0.26303353962298526	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	1349	0.19200879055812073	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	1262	0.1796257180758698	No Hit
AACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1035	0.14731586228884727	Illumina PCR Primer Index 7 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	906	0.12895475481516486	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	849	0.12084170732679356	RNA PCR Primer, Index 14 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATATC	837	0.1191336973292417	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTAT	771	0.10973964234270651	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	711	0.10119959235494724	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGA	30	2.1642918E-6	45.000004	41
CCCTCGA	20	7.0314115E-4	45.000004	15
TTGGACG	20	7.0314115E-4	45.000004	1
CCTAGCG	20	7.0314115E-4	45.000004	1
CGTTGCC	35	1.2110286E-7	45.000004	30
TAGCGGA	40	6.8084773E-9	45.000004	3
TAGCGCG	30	2.1642918E-6	45.000004	1
TTCGTAC	30	2.1642918E-6	45.000004	33
TAGCCGT	30	2.1642918E-6	45.000004	44
CTAGTAA	20	7.0314115E-4	45.000004	14
AATTCGT	30	2.1642918E-6	45.000004	31
TCCGTGA	20	7.0314115E-4	45.000004	17
GCTAGCG	20	7.0314115E-4	45.000004	1
TATAGCG	30	2.1642918E-6	45.000004	1
ATCGGCG	20	7.0314115E-4	45.000004	11
ATACGCA	20	7.0314115E-4	45.000004	34
CGTCCTA	20	7.0314115E-4	45.000004	42
GATTACG	20	7.0314115E-4	45.000004	1
TATCGGG	30	2.1642918E-6	45.000004	3
CGATATG	40	6.8084773E-9	45.000004	1