##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702572
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77407724759882	31.0	31.0	34.0	30.0	34.0
2	31.971033573783185	33.0	31.0	34.0	30.0	34.0
3	32.13186406517766	34.0	31.0	34.0	30.0	34.0
4	35.78547679099081	37.0	35.0	37.0	35.0	37.0
5	35.54705282874922	37.0	35.0	37.0	33.0	37.0
6	34.891433191188945	37.0	35.0	37.0	32.0	37.0
7	35.26784870447442	37.0	35.0	37.0	33.0	37.0
8	35.690007572177656	37.0	35.0	37.0	33.0	37.0
9	37.3681131613557	39.0	37.0	39.0	34.0	39.0
10	36.935968128533446	39.0	37.0	39.0	33.0	39.0
11	36.93276133976304	39.0	37.0	39.0	33.0	39.0
12	36.774343412490104	39.0	35.0	39.0	33.0	39.0
13	36.75687474024014	39.0	35.0	39.0	33.0	39.0
14	37.90523106528583	40.0	37.0	41.0	33.0	41.0
15	37.96526049999146	40.0	37.0	41.0	33.0	41.0
16	37.793823266512184	40.0	37.0	41.0	33.0	41.0
17	37.7835140028353	40.0	37.0	41.0	33.0	41.0
18	37.760729149467956	40.0	36.0	41.0	33.0	41.0
19	37.753659411419754	40.0	36.0	41.0	33.0	41.0
20	37.71150003131352	40.0	36.0	41.0	33.0	41.0
21	37.71455167584248	40.0	36.0	41.0	33.0	41.0
22	37.71395814236833	40.0	36.0	41.0	33.0	41.0
23	37.63142567594495	39.0	36.0	41.0	33.0	41.0
24	37.47833958654771	39.0	36.0	41.0	32.0	41.0
25	37.519475583997085	39.0	36.0	41.0	32.0	41.0
26	37.483415222923774	39.0	36.0	41.0	32.0	41.0
27	37.38673331701235	39.0	36.0	41.0	32.0	41.0
28	37.29644648520009	39.0	36.0	41.0	32.0	41.0
29	37.13398626759962	39.0	35.0	41.0	31.0	41.0
30	37.18746548396463	39.0	35.0	41.0	32.0	41.0
31	37.15074326901727	39.0	35.0	41.0	31.0	41.0
32	37.119967775544715	39.0	35.0	41.0	31.0	41.0
33	36.89030732793223	39.0	35.0	41.0	31.0	41.0
34	36.881763007919474	39.0	35.0	41.0	31.0	41.0
35	36.834861053386696	39.0	35.0	41.0	31.0	41.0
36	36.85639336608917	39.0	35.0	41.0	31.0	41.0
37	36.760169776193756	39.0	35.0	41.0	31.0	41.0
38	36.577096724606164	39.0	35.0	40.0	30.0	41.0
39	36.56226692780242	39.0	35.0	40.0	30.0	41.0
40	36.41883536491633	39.0	35.0	40.0	30.0	41.0
41	36.44578633933604	39.0	35.0	40.0	30.0	41.0
42	36.38653120249597	39.0	35.0	40.0	30.0	41.0
43	36.3354688202775	38.0	35.0	40.0	30.0	41.0
44	36.26265208405687	38.0	35.0	40.0	30.0	41.0
45	36.03050505855628	38.0	35.0	40.0	30.0	41.0
46	35.85124656262988	38.0	35.0	40.0	29.0	41.0
47	35.74731557762051	38.0	34.0	40.0	29.0	41.0
48	35.64922741014444	38.0	34.0	40.0	28.0	41.0
49	35.5422775174644	38.0	34.0	40.0	28.0	41.0
50	35.45453419720683	37.0	34.0	40.0	28.0	41.0
51	34.144067796610166	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	1.0
11	11.0
12	4.0
13	9.0
14	14.0
15	27.0
16	67.0
17	131.0
18	265.0
19	457.0
20	886.0
21	1401.0
22	1989.0
23	2601.0
24	3275.0
25	3896.0
26	4717.0
27	5887.0
28	7353.0
29	9635.0
30	12730.0
31	16987.0
32	22329.0
33	30573.0
34	47372.0
35	58229.0
36	65584.0
37	94498.0
38	141268.0
39	170331.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.477007338749623	33.418496609600155	20.382252637452105	26.722243414198115
2	22.738310094908424	33.130981593345595	19.47188330875697	24.65882500298902
3	20.048194348764255	30.89320382821974	23.32116850657299	25.737433316443013
4	23.01102235785087	30.09969085019044	18.79536901555997	28.09391777639872
5	18.237419083026367	34.68655169861594	17.98548760838747	29.090541609970224
6	19.514014222029914	36.80177974641745	21.401080600991783	22.283125430560855
7	81.55021834061135	7.781266546346852	6.088628638772966	4.57988647426883
8	83.31630067807997	4.5542663243055514	4.739585409039927	7.389847588574552
9	77.63617109705481	8.167419139960034	8.722095386664996	5.47431437632015
10	36.17892543397687	33.07091657509835	14.052082918192013	16.69807507273276
11	27.22696036847469	27.365024509943463	24.900081415143216	20.507933706438628
12	27.227672039307006	22.475561223618364	30.21797054252091	20.078796194553725
13	25.899124929544588	25.021634793302322	26.08928337593869	22.989956901214395
14	21.00724196238962	26.092130059267948	30.21853987918676	22.682088099155674
15	20.390223350774015	27.989159829882205	27.938773534954425	23.68184328438936
16	22.320132313841142	26.96093781135599	28.16053016630324	22.55839970849963
17	20.591626196318668	27.071246790364544	28.26500344448683	24.072123568829955
18	22.9259065263062	26.43330505627893	26.292394231480902	24.34839418593397
19	22.9602090604237	29.519536787688665	26.928058618903115	20.59219553298452
20	25.450202968521374	29.249386539742545	24.465677539099197	20.834732952636884
21	24.534424941500657	26.58631428522628	25.940686506151682	22.93857426712138
22	21.198396747948962	27.443450635664384	25.407075716083195	25.951076900303455
23	23.200753801745584	27.095301264496737	24.357788240920502	25.346156692837173
24	21.53117402913865	29.37933763372295	25.6467664524063	23.442721884732098
25	24.238654543591263	26.57378887857757	23.708459773517873	25.479096804313293
26	25.013806414146877	27.362320160780673	22.984975205388203	24.638898219684247
27	21.082679070614827	25.877632470408727	26.514435531162643	26.525252927813803
28	24.004372505593732	26.55557010527035	26.145078369192053	23.294979019943863
29	22.608643669260946	27.932653165796527	24.42041527416407	25.038287890778456
30	24.59605563557899	24.76144793700859	26.107502149245914	24.534994278166508
31	24.724156385395375	26.283427178993758	22.805918823978182	26.186497611632685
32	23.573811652044203	25.42956451438429	24.088776666306085	26.90784716726542
33	24.371309986734456	25.533326121735566	24.68771883878094	25.40764505274904
34	20.784915994374952	25.307584133725797	26.941011028051214	26.96648884384803
35	22.651628587532667	26.927489282237264	25.647762791571537	24.773119338658528
36	21.7280221813565	27.797720375989933	26.223789163245904	24.25046827940766
37	21.341584919410394	28.967280221813564	23.960960584822626	25.730174273953416
38	22.64750089670525	27.802702071816128	25.32708391453118	24.222713116947446
39	21.36236570771394	25.54855587754707	26.339364506413578	26.74971390832541
40	22.621026741743194	27.4559760423131	25.66868591404155	24.254311301902153
41	20.67375301036762	25.205388202205608	29.42004520533127	24.700813582095503
42	22.677106403329482	27.242759460952044	25.403232693588702	24.676901442129775
43	21.800043269586606	24.55492106147128	27.302824479199284	26.34221118974283
44	23.20730117340287	22.291238478049223	26.514720199495567	27.98674014905234
45	25.333631286188464	24.07867094048724	26.995382679639953	23.59231509368435
46	21.31724577694528	23.797987964222884	29.460468108606662	25.424298150225173
47	21.286928599488736	27.034951577916573	25.21350124969398	26.46461857290071
48	21.31240641528555	24.032412336386876	30.360304708983566	24.29487653934401
49	22.705146234122626	24.608438708061236	27.32787529249671	25.358539765319428
50	21.846728876186354	23.0996965435571	27.50579300057503	27.547781579681512
51	20.80285009934925	22.493495328592655	30.779478829216078	25.924175742842014
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2347.0
1	1907.0
2	1467.0
3	1016.5
4	566.0
5	566.0
6	566.0
7	569.0
8	572.0
9	645.0
10	718.0
11	781.5
12	845.0
13	853.0
14	861.0
15	935.0
16	1009.0
17	1051.5
18	1094.0
19	1319.0
20	1544.0
21	1372.5
22	1201.0
23	2241.0
24	3281.0
25	3561.0
26	4698.5
27	5556.0
28	6234.0
29	6912.0
30	7999.0
31	9086.0
32	11528.5
33	13971.0
34	15218.0
35	16465.0
36	17848.5
37	19232.0
38	20438.0
39	21644.0
40	24212.5
41	26781.0
42	29166.5
43	31552.0
44	44205.0
45	56858.0
46	58564.0
47	60270.0
48	62967.0
49	65664.0
50	63277.5
51	60891.0
52	56267.5
53	51644.0
54	47481.5
55	43319.0
56	40625.5
57	37932.0
58	35958.5
59	33985.0
60	32693.5
61	31402.0
62	27926.0
63	24450.0
64	21737.0
65	19024.0
66	16230.5
67	13437.0
68	12180.5
69	10924.0
70	8610.5
71	6297.0
72	5597.0
73	4897.0
74	4614.5
75	3541.0
76	2750.0
77	2013.5
78	1277.0
79	1113.5
80	950.0
81	821.5
82	693.0
83	439.5
84	186.0
85	174.0
86	162.0
87	104.5
88	47.0
89	39.5
90	32.0
91	29.0
92	26.0
93	17.5
94	9.0
95	5.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	702572.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.79404227725932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.12430216121415	18.62635123834747
2	10.25553605547462	5.085523890707899
3	3.7978094215381097	2.8248914207576896
4	1.8411676724960906	1.8259995644556481
5	1.0364826385999706	1.2849297180546482
6	0.7180848035219878	1.068253498630897
7	0.5365118800526836	0.9311608765394682
8	0.40654523593718456	0.8063919813955935
9	0.32053950721784186	0.7152723084141936
>10	4.116113038770668	25.006860994830383
>50	1.4995507193315487	26.17059989394867
>100	0.33739891184959847	11.072210393765483
>500	0.005271857997649976	0.9370502703487138
>1k	0.0035145719984333175	2.0637762463817766
>5k	0.0011715239994777725	1.5807277034214817
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	5506	0.7836919205433749	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	5378	0.7654731472361551	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	4991	0.7103898248151079	RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	3725	0.5301947700733876	Illumina PCR Primer Index 7 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1848	0.26303353962298526	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	1349	0.19200879055812073	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	1262	0.1796257180758698	No Hit
AACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1035	0.14731586228884727	Illumina PCR Primer Index 7 (95% over 21bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	906	0.12895475481516486	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	849	0.12084170732679356	RNA PCR Primer, Index 14 (95% over 22bp)
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATATC	837	0.1191336973292417	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTAT	771	0.10973964234270651	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	711	0.10119959235494724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5996538433071629	0.0
2	0.0	0.0	0.0	0.8298081904772749	0.0
3	0.0	0.0	0.0	1.2233621607465142	0.0
4	0.0	0.0	0.0	2.9994078898675154	0.0
5	0.0	0.0	0.0	3.2455036636814447	0.0
6	0.0	0.0	0.0	4.464026462768229	0.0
7	0.0	0.0	0.0	5.4346031438770686	0.0
8	0.0	0.0	0.0	6.1707554528219175	0.0
9	0.0	0.0	0.0	7.75920475054514	0.0
10	0.0	0.0	0.0	9.111379331940356	0.0
11	0.0	0.0	0.0	10.99858804506869	0.0
12	0.0	0.0	0.0	11.592406187550884	0.0
13	0.0	0.0	0.0	11.888461253793206	0.0
14	0.0	0.0	0.0	12.277175862402714	0.0
15	0.0	0.0	0.0	12.537789721195836	0.0
16	0.0	0.0	0.0	13.017028859675593	0.0
17	0.0	0.0	0.0	13.560176038897081	0.0
18	0.0	0.0	0.0	14.517373308358431	0.0
19	0.0	0.0	0.0	14.84915425038288	0.0
20	0.0	0.0	0.0	15.23046748233633	0.0
21	0.0	0.0	0.0	15.633557841758567	0.0
22	0.0	0.0	0.0	16.03864087951128	0.0
23	0.0	0.0	0.0	16.472190750556525	0.0
24	0.0	0.0	0.0	16.796997318424303	0.0
25	0.0	0.0	0.0	17.0691402447009	0.0
26	0.0	0.0	0.0	17.32377606850259	0.0
27	0.0	0.0	0.0	17.623247154740014	0.0
28	0.0	0.0	0.0	17.904926470169606	0.0
29	0.0	0.0	0.0	18.20553622973873	0.0
30	0.0	0.0	0.0	18.642644454945543	0.0
31	0.0	0.0	0.0	18.927597456203777	0.0
32	0.0	0.0	0.0	19.22109050744977	0.0
33	0.0	0.0	0.0	19.534652676167	0.0
34	0.0	0.0	0.0	19.804945258279577	0.0
35	0.0	0.0	0.0	20.11551840950109	0.0
36	0.0	0.0	0.0	20.400044408259937	0.0
37	0.0	0.0	0.0	20.68442807285232	0.0
38	0.0	0.0	0.0	20.969381074110554	0.0
39	0.0	0.0	0.0	21.296464988641734	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGA	30	2.1642918E-6	45.000004	41
CCCTCGA	20	7.0314115E-4	45.000004	15
TTGGACG	20	7.0314115E-4	45.000004	1
CCTAGCG	20	7.0314115E-4	45.000004	1
CGTTGCC	35	1.2110286E-7	45.000004	30
TAGCGGA	40	6.8084773E-9	45.000004	3
TAGCGCG	30	2.1642918E-6	45.000004	1
TTCGTAC	30	2.1642918E-6	45.000004	33
TAGCCGT	30	2.1642918E-6	45.000004	44
CTAGTAA	20	7.0314115E-4	45.000004	14
AATTCGT	30	2.1642918E-6	45.000004	31
TCCGTGA	20	7.0314115E-4	45.000004	17
GCTAGCG	20	7.0314115E-4	45.000004	1
TATAGCG	30	2.1642918E-6	45.000004	1
ATCGGCG	20	7.0314115E-4	45.000004	11
ATACGCA	20	7.0314115E-4	45.000004	34
CGTCCTA	20	7.0314115E-4	45.000004	42
GATTACG	20	7.0314115E-4	45.000004	1
TATCGGG	30	2.1642918E-6	45.000004	3
CGATATG	40	6.8084773E-9	45.000004	1
>>END_MODULE