SRR3548449_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713510
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	6373	0.8931900043447183	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	5999	0.840773079564407	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	5606	0.7856932628834915	RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	3933	0.5512186234250396	Illumina PCR Primer Index 7 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1897	0.2658687334445208	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	1579	0.22130033216072653	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	1350	0.18920547714818292	No Hit
AACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1100	0.15416742582444534	Illumina PCR Primer Index 7 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	924	0.1295006376925341	RNA PCR Primer, Index 14 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	917	0.12851957225546945	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATATC	907	0.12711805020251993	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTAT	901	0.12627713697075024	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	720	0.10090958781236424	Illumina PCR Primer Index 7 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	718	0.10062928340177434	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	50	2.1827873E-11	45.000004	25
TAGCCGT	25	3.8893122E-5	45.000004	44
CCACTAG	35	1.211065E-7	45.000004	1
TACGAAG	35	1.211065E-7	45.000004	1
ACGCTAA	25	3.8893122E-5	45.000004	11
TTACGTT	30	2.164339E-6	45.000004	32
TATCGCG	85	0.0	45.000004	1
TAACTCG	25	3.8893122E-5	45.000004	37
TGATCGC	20	7.031486E-4	45.0	21
TCACGAG	20	7.031486E-4	45.0	1
AAATCGT	20	7.031486E-4	45.0	36
CGGCATA	20	7.031486E-4	45.0	1
TTCGTAC	40	6.8084773E-9	45.0	33
TCGATAA	20	7.031486E-4	45.0	31
CCTCGCG	20	7.031486E-4	45.0	1
CCGTCCA	20	7.031486E-4	45.0	30
ACGGCAC	20	7.031486E-4	45.0	5
CGCTAGC	20	7.031486E-4	45.0	13
TCGCTAA	20	7.031486E-4	45.0	44
AACGATA	20	7.031486E-4	45.0	37