##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713510
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.780716458073467	31.0	31.0	34.0	30.0	34.0
2	31.980968732043	33.0	31.0	34.0	30.0	34.0
3	32.117301789743664	34.0	31.0	34.0	30.0	34.0
4	35.775059915067764	37.0	35.0	37.0	35.0	37.0
5	35.54543033734636	37.0	35.0	37.0	33.0	37.0
6	35.56042802483497	37.0	35.0	37.0	33.0	37.0
7	35.60355145688217	37.0	35.0	37.0	33.0	37.0
8	35.68370590461241	37.0	35.0	37.0	35.0	37.0
9	37.384829925298874	39.0	37.0	39.0	35.0	39.0
10	36.94718223991254	39.0	37.0	39.0	33.0	39.0
11	36.95849812896806	39.0	37.0	39.0	33.0	39.0
12	36.82164790962986	39.0	35.0	39.0	33.0	39.0
13	36.80557385320458	39.0	35.0	39.0	33.0	39.0
14	37.94420120250592	40.0	37.0	41.0	33.0	41.0
15	38.002748384745836	40.0	37.0	41.0	33.0	41.0
16	37.84288937786436	40.0	37.0	41.0	33.0	41.0
17	37.80016397808019	40.0	37.0	41.0	33.0	41.0
18	37.7302196185057	40.0	36.0	41.0	33.0	41.0
19	37.74426006643215	40.0	36.0	41.0	33.0	41.0
20	37.722701854213675	40.0	36.0	41.0	33.0	41.0
21	37.72064021527379	40.0	36.0	41.0	33.0	41.0
22	37.700529775336015	39.0	36.0	41.0	33.0	41.0
23	37.59400008409132	39.0	36.0	41.0	32.0	41.0
24	37.450512256310354	39.0	36.0	41.0	32.0	41.0
25	37.499257193311934	39.0	36.0	41.0	32.0	41.0
26	37.45261173634567	39.0	36.0	41.0	32.0	41.0
27	37.38815854017463	39.0	36.0	41.0	32.0	41.0
28	37.29108912278735	39.0	36.0	41.0	32.0	41.0
29	37.16201034323275	39.0	35.0	41.0	32.0	41.0
30	37.214619276534314	39.0	36.0	41.0	32.0	41.0
31	37.16090173928887	39.0	35.0	41.0	32.0	41.0
32	37.11863043264986	39.0	35.0	41.0	31.0	41.0
33	37.03793639892924	39.0	35.0	41.0	31.0	41.0
34	36.969815419545625	39.0	35.0	41.0	31.0	41.0
35	36.891590867682304	39.0	35.0	41.0	31.0	41.0
36	36.86112177825118	39.0	35.0	41.0	31.0	41.0
37	36.77242084904206	39.0	35.0	41.0	31.0	41.0
38	36.696357444184386	39.0	35.0	40.0	31.0	41.0
39	36.641613992796174	39.0	35.0	40.0	31.0	41.0
40	36.45045619542824	39.0	35.0	40.0	30.0	41.0
41	36.44518366946504	39.0	35.0	40.0	30.0	41.0
42	36.37692253787613	39.0	35.0	40.0	30.0	41.0
43	36.31151630670909	38.0	35.0	40.0	30.0	41.0
44	36.242391837535564	38.0	35.0	40.0	30.0	41.0
45	36.02798699387535	38.0	35.0	40.0	30.0	41.0
46	35.86197110061527	38.0	35.0	40.0	29.0	41.0
47	35.7413631203487	38.0	34.0	40.0	29.0	41.0
48	35.654798110748274	38.0	34.0	40.0	28.0	41.0
49	35.4894731678603	38.0	34.0	40.0	28.0	41.0
50	35.41418340317585	37.0	34.0	40.0	28.0	41.0
51	34.001911676080226	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	6.0
12	6.0
13	8.0
14	21.0
15	31.0
16	78.0
17	120.0
18	260.0
19	512.0
20	798.0
21	1284.0
22	1866.0
23	2502.0
24	3147.0
25	4032.0
26	4742.0
27	5920.0
28	7661.0
29	9752.0
30	12897.0
31	16854.0
32	22500.0
33	30562.0
34	47760.0
35	58825.0
36	66388.0
37	95168.0
38	145309.0
39	174443.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.440652548667853	33.735616879931605	20.32697509495312	26.49675547644742
2	22.630797045591514	33.398410673991954	19.406175106165296	24.56461717425124
3	20.036860029992575	30.702863309554175	23.55397962186935	25.7062970385839
4	23.196731650572524	29.94590124875615	18.790066011688694	28.06730108898263
5	18.104861880001682	34.71065577216857	18.025675884010035	29.158806463819708
6	19.869938753486284	35.53671287017701	22.06556320163698	22.527785174699723
7	81.23375986321145	7.866883435410855	6.261580075962496	4.6377766254152
8	82.74011576572158	4.638337234236381	4.868887611946574	7.752659388095472
9	77.30178974366162	8.119157404941767	8.972824487393309	5.606228364003307
10	35.93138148028759	33.01621561015263	14.17289176045185	16.87951114910793
11	27.1469215568107	27.082591694580316	25.202730164959146	20.567756583649842
12	26.90922341663046	22.486860730753598	30.61554848565542	19.988367366960517
13	25.939370155989405	24.87014898179423	26.107552802343342	23.082928059873023
14	20.881277066894647	25.89802525542739	30.69277235077294	22.52792532690502
15	20.296562066404114	27.899679051449876	27.93709969026363	23.866659191882384
16	22.01076368936665	26.863113341088425	28.561898221468514	22.564224748076413
17	20.404198960070637	27.123656290731734	28.411795209597624	24.060349539600008
18	22.793653908144247	26.49423273675211	26.323807655113452	24.38830569999019
19	22.736752112794495	29.59355860464464	27.159254950876655	20.51043433168421
20	25.274488094070158	29.20561730038822	24.643102409216407	20.87679219632521
21	24.298187831985537	26.593600650306232	26.04462446216591	23.06358705554232
22	20.952614539389778	27.648105842945437	25.49298538212499	25.906294235539796
23	23.140110159633362	26.94720466426539	24.364900281705935	25.547784894395313
24	21.391991702989447	29.441493461899622	25.75759274572185	23.408922089389076
25	24.18480469790192	26.44139535535592	23.81928774649269	25.55451220024947
26	24.819133579066865	27.42512368432117	22.981037406623596	24.774705329988368
27	20.90678476825833	25.841684068898825	26.61476363330577	26.636767529537074
28	24.136732491485756	26.370198035066082	26.07251475101961	23.42055472242856
29	22.432201370688567	27.93808075570069	24.49650320247789	25.133214671132855
30	24.51430253255035	24.58998472340962	26.22696248125464	24.668750262785384
31	24.549620888284675	26.320584154391668	22.731146024582696	26.398648932740958
32	23.282084343597145	25.397401578113833	24.11066418130089	27.209849896988132
33	24.377513980182478	25.593614665526758	24.58199604770781	25.44687530658295
34	20.59172261075528	25.171476223178374	26.90922341663046	27.32757774943589
35	22.447898417681603	26.964583537721964	25.777634511079032	24.8098835335174
36	21.67776204958585	27.57536684839736	26.38183066810556	24.36504043391123
37	21.175736850219337	28.84038065338958	24.095387590923742	25.888494905467336
38	22.56198231279169	27.73051533965887	25.427534302252248	24.279968045297192
39	21.164244369385152	25.347647545234125	26.598505977491556	26.889602107889164
40	22.53367156732211	27.72911381760592	25.598940449327973	24.138274165743997
41	20.561309582206274	24.921444688932183	29.75879805468739	24.758447674174153
42	22.638505416882733	27.18812630516741	25.42375019270928	24.74961808524057
43	21.82548247396673	24.368123782427716	27.354206668441925	26.45218707516363
44	23.019999719695587	22.144188588807445	26.624013678855235	28.21179801264173
45	25.431738868411095	23.961822539277655	26.978879062662052	23.6275595296492
46	21.15317234516685	23.58565402026601	29.863772056453307	25.397401578113833
47	21.258006194727475	27.016439853681096	25.191097531919667	26.534456419671766
48	21.136354080531458	23.88950400134546	30.657313842833318	24.316828075289763
49	22.608793149360203	24.537988255245196	27.386721980070355	25.466496615324242
50	21.754285153676893	22.719092934927332	27.58307521968858	27.943546691707194
51	20.67497302070048	22.32134097630026	31.114770640916035	25.88891536208322
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2400.0
1	1956.5
2	1513.0
3	1043.0
4	573.0
5	578.5
6	584.0
7	627.5
8	671.0
9	703.0
10	735.0
11	779.5
12	824.0
13	803.5
14	783.0
15	895.5
16	1008.0
17	1020.5
18	1033.0
19	1333.5
20	1634.0
21	1414.0
22	1194.0
23	2185.5
24	3177.0
25	3498.5
26	4764.0
27	5708.0
28	6265.5
29	6823.0
30	7882.5
31	8942.0
32	11452.0
33	13962.0
34	15249.5
35	16537.0
36	18231.5
37	19926.0
38	20822.0
39	21718.0
40	24389.5
41	27061.0
42	29691.5
43	32322.0
44	45610.5
45	58899.0
46	61016.0
47	63133.0
48	65361.5
49	67590.0
50	64709.0
51	61828.0
52	57002.0
53	52176.0
54	48011.0
55	43846.0
56	40997.0
57	38148.0
58	36003.0
59	33858.0
60	32771.5
61	31685.0
62	28123.0
63	24561.0
64	21760.0
65	18959.0
66	16174.0
67	13389.0
68	12113.5
69	10838.0
70	8620.0
71	6402.0
72	5612.5
73	4823.0
74	4570.5
75	3534.0
76	2750.0
77	2028.5
78	1307.0
79	1115.5
80	924.0
81	795.5
82	667.0
83	431.0
84	195.0
85	171.0
86	147.0
87	95.0
88	43.0
89	38.5
90	34.0
91	32.5
92	31.0
93	19.5
94	8.0
95	4.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	713510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.738407592442716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.30422268126156	18.629065541211183
2	10.143241845886644	5.018553021845296
3	3.6904095145823907	2.7388456426430356
4	1.8713088145431853	1.8517280074200047
5	1.0736499674753592	1.328019525350915
6	0.7208139552632233	1.0699073654173434
7	0.5185420422239609	0.8979533076047789
8	0.41765000564892296	0.8265596856583247
9	0.3351760297342516	0.7462549114904353
>10	4.1014599440492105	24.78375764302998
>50	1.4590486713947646	25.501768172036076
>100	0.3547647709225327	11.706983048024917
>500	0.0051415184191671405	0.9665253095288626
>1k	0.0028563991217595225	1.3933284144823888
>5k	0.0017138394730557133	2.5407504042564546
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	6373	0.8931900043447183	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGT	5999	0.840773079564407	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	5606	0.7856932628834915	RNA PCR Primer, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGCT	3933	0.5512186234250396	Illumina PCR Primer Index 7 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1897	0.2658687334445208	No Hit
AATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	1579	0.22130033216072653	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	1350	0.18920547714818292	No Hit
AACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTG	1100	0.15416742582444534	Illumina PCR Primer Index 7 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	924	0.1295006376925341	RNA PCR Primer, Index 14 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	917	0.12851957225546945	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATATC	907	0.12711805020251993	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTAT	901	0.12627713697075024	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	720	0.10090958781236424	Illumina PCR Primer Index 7 (95% over 22bp)
AAAAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	718	0.10062928340177434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6360107076284846	0.0
2	0.0	0.0	0.0	0.8874437639276254	0.0
3	0.0	0.0	0.0	1.3100026628919006	0.0
4	0.0	0.0	0.0	3.2211181342938433	0.0
5	0.0	0.0	0.0	3.4902103684601475	0.0
6	0.0	0.0	0.0	4.79460694314025	0.0
7	0.0	0.0	0.0	5.798657341873274	0.0
8	0.0	0.0	0.0	6.549032249022439	0.0
9	0.0	0.0	0.0	8.27865061456742	0.0
10	0.0	0.0	0.0	9.682835559417528	0.0
11	0.0	0.0	0.0	11.715042536194307	0.0
12	0.0	0.0	0.0	12.335776653445642	0.0
13	0.0	0.0	0.0	12.645793331558073	0.0
14	0.0	0.0	0.0	13.052094574708134	0.0
15	0.0	0.0	0.0	13.304368544239043	0.0
16	0.0	0.0	0.0	13.816344550181498	0.0
17	0.0	0.0	0.0	14.40218076831439	0.0
18	0.0	0.0	0.0	15.401465992067385	0.0
19	0.0	0.0	0.0	15.729001695841685	0.0
20	0.0	0.0	0.0	16.129276394164062	0.0
21	0.0	0.0	0.0	16.54230494316828	0.0
22	0.0	0.0	0.0	16.955753948788384	0.0
23	0.0	0.0	0.0	17.395691721209232	0.0
24	0.0	0.0	0.0	17.721405446314698	0.0
25	0.0	0.0	0.0	17.99358102899749	0.0
26	0.0	0.0	0.0	18.24753682499194	0.0
27	0.0	0.0	0.0	18.55236787150846	0.0
28	0.0	0.0	0.0	18.837717761488978	0.0
29	0.0	0.0	0.0	19.151098092528485	0.0
30	0.0	0.0	0.0	19.593418452439348	0.0
31	1.401522052949503E-4	0.0	0.0	19.883113060784012	0.0
32	1.401522052949503E-4	0.0	0.0	20.19453126094939	0.0
33	1.401522052949503E-4	0.0	0.0	20.51127524491598	0.0
34	2.803044105899006E-4	0.0	0.0	20.785272806267606	0.0
35	2.803044105899006E-4	0.0	0.0	21.09991450715477	0.0
36	2.803044105899006E-4	0.0	0.0	21.403904640439517	0.0
37	2.803044105899006E-4	0.0	0.0	21.676921136354082	0.0
38	2.803044105899006E-4	0.0	0.0	21.982452943897073	0.0
39	2.803044105899006E-4	0.0	0.0	22.302981037406624	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAT	50	2.1827873E-11	45.000004	25
TAGCCGT	25	3.8893122E-5	45.000004	44
CCACTAG	35	1.211065E-7	45.000004	1
TACGAAG	35	1.211065E-7	45.000004	1
ACGCTAA	25	3.8893122E-5	45.000004	11
TTACGTT	30	2.164339E-6	45.000004	32
TATCGCG	85	0.0	45.000004	1
TAACTCG	25	3.8893122E-5	45.000004	37
TGATCGC	20	7.031486E-4	45.0	21
TCACGAG	20	7.031486E-4	45.0	1
AAATCGT	20	7.031486E-4	45.0	36
CGGCATA	20	7.031486E-4	45.0	1
TTCGTAC	40	6.8084773E-9	45.0	33
TCGATAA	20	7.031486E-4	45.0	31
CCTCGCG	20	7.031486E-4	45.0	1
CCGTCCA	20	7.031486E-4	45.0	30
ACGGCAC	20	7.031486E-4	45.0	5
CGCTAGC	20	7.031486E-4	45.0	13
TCGCTAA	20	7.031486E-4	45.0	44
AACGATA	20	7.031486E-4	45.0	37
>>END_MODULE